MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
432 358.2031 714.3916 714.4024 -15.1 0 15 0.52 +1Score > 42 indicates identity
Score > 25 indicates homology
VNNLQK
1751 436.9161 1307.7265 1307.7350 -6.51 0 15 0.46 +1Score > 37 indicates identity
Score > 24 indicates homology
IAGPGFINIHLR + Deamidated (NQ)
1184 518.2497 1034.4848 1034.4741 10.4 1 15 0.68 +1Score > 41 indicates identity
Score > 26 indicates homology
RADSSASQGR + Deamidated (NQ)
1770 660.3846 1318.7546 1318.7497 3.78 1 15 0.31 +1Score > 35 indicates identity
Score > 23 indicates homology
FLGTTKDPVTLK
3549 567.5179 2266.0425 2266.0585 -7.04 1 15 0.29 +1Score > 38 indicates identity
Score > 22 indicates homology
CQLQSLGFMKVFMYLNDR + Deamidated (NQ); Oxidation (M)
1648 630.3170 1258.6194 1258.6153 3.30 1 15 0.59 +1Score > 41 indicates identity
Score > 25 indicates homology
QASEIIADAANR + Deamidated (NQ)
737 427.7428 853.4710 853.4770 -6.96 1 15 0.36 +1Score > 37 indicates identity
Score > 23 indicates homology
TQPAPRGK
1295 551.2972 1100.5798 1100.5713 7.72 1 15 0.71 +1Score > 41 indicates identity
Score > 26 indicates homology
AVVVIGDENGK + Deamidated (NQ)
1464 586.8333 1171.6520 1171.6713 -16.4 0 15 0.68 +1Score > 40 indicates identity
Score > 26 indicates homology
NIALLPFASAR
1501 597.3433 1192.6720 1192.6928 -17.4 1 15 0.82 +1Score > 37 indicates identity
Score > 27 indicates homology
LPEVQRPVVR + Deamidated (NQ)
3105 673.7194 2018.1364 2018.1008 17.6 1 15 0.068 +1Score > 33 indicates identity
Score > 16 indicates homology
ITKTANTITVSGTGNITGIR + Deamidated (NQ)
3151 511.5270 2042.0789 2042.0585 10.0 1 15 0.37 +1Score > 38 indicates identity
Score > 23 indicates homology
SLADIQARYPHLPYQLR + 2 Deamidated (NQ)
1360 566.7972 1131.5798 1131.5659 12.3 1 15 0.43 +1Score > 42 indicates identity
Score > 24 indicates homology
ATVKQINNLE + 3 Deamidated (NQ)
3185 1031.5519 2061.0892 2061.0525 17.8 1 15 0.6 +1Score > 38 indicates identity
Score > 26 indicates homology
VSTANGRMVLTVTDDDLVR
536 382.1737 762.3328 762.3396 -8.80 0 15 0.77 +1Score > 40 indicates identity
Score > 27 indicates homology
TANDVDK + Deamidated (NQ)
828 447.7390 893.4634 893.4463 19.2 1 15 0.83 +1Score > 41 indicates identity
Score > 27 indicates homology
ASVKAMCK
2081 736.8672 1471.7198 1471.7381 -12.4 0 15 0.35 +1Score > 40 indicates identity
Score > 23 indicates homology
WALMIDPQGQALK + 2 Deamidated (NQ)
2437 543.6175 1627.8307 1627.8239 4.14 1 15 0.89 +1Score > 40 indicates identity
Score > 27 indicates homology
SRDLAIQLGMLDPAE
1290 366.9027 1097.6863 1097.6920 -5.26 1 15 0.51 +1Score > 33 indicates identity
Score > 25 indicates homology
KVLALALQSR
3039 990.4906 1978.9666 1978.9636 1.54 1 15 0.16 +1Score > 40 indicates identity
Score > 20 indicates homology
SVPYQGDPAAYSQELAVGK
2144 752.3774 1502.7402 1502.7576 -11.6 0 15 0.82 +1Score > 41 indicates identity
Score > 27 indicates homology
SQVGPVLSSNASTVR + 2 Deamidated (NQ)
3436 1099.0770 2196.1394 2196.1498 -4.73 0 15 0.81 +1Score > 38 indicates identity
Score > 27 indicates homology
GNNINPLVSASTVQTGINIAGR + Deamidated (NQ)
650 407.2292 812.4438 812.4504 -8.09 1 15 0.84 +1Score > 39 indicates identity
Score > 27 indicates homology
KAPASSPR
767 434.2059 866.3972 866.4134 -18.6 1 15 0.28 +1Score > 41 indicates identity
Score > 22 indicates homology
VENSVYR + Deamidated (NQ)
1155 1021.5498 1020.5425 1020.5240 18.2 1 15 0.4 +1Score > 42 indicates identity
Score > 24 indicates homology
YIQQALER + Deamidated (NQ)
1786 661.8786 1321.7426 1321.7354 5.49 0 15 0.43 +1Score > 36 indicates identity
Score > 24 indicates homology
QVASLPDPVGAIR
2847 922.9919 1843.9692 1843.9415 15.1 0 15 0.29 +1Score > 39 indicates identity
Score > 22 indicates homology
TANGATIDQIAQLIDGLK + 3 Deamidated (NQ)
3200 692.3538 2074.0396 2074.0252 6.92 1 15 0.88 +1Score > 39 indicates identity
Score > 27 indicates homology
LMINTSTNEPDILVDEVR + Oxidation (M)
285 319.1698 636.3250 636.3231 3.05 0 15 0.45 +1Score > 41 indicates identity
Score > 24 indicates homology
YIADR
3914 639.3309 2553.2945 2553.2534 16.1 0 15 0.24 +1Score > 38 indicates identity
Score > 21 indicates homology
GTLPVVQLMDFHDAIGLNGNGSGPK + Deamidated (NQ); Oxidation (M)
1413 578.2904 1154.5662 1154.5641 1.83 0 15 0.21 +1Score > 40 indicates identity
Score > 21 indicates homology
QMIGYNLATK + Deamidated (NQ); Oxidation (M)
2078 735.9210 1469.8274 1469.8388 -7.71 1 15 0.35 +1Score > 37 indicates identity
Score > 23 indicates homology
LTAPLAMKLGAAAAR + Oxidation (M)
445 361.2171 720.4196 720.4283 -11.9 1 15 0.44 +1Score > 38 indicates identity
Score > 24 indicates homology
VVYGKR
1401 575.7781 1149.5416 1149.5554 -11.9 1 15 0.63 +1Score > 41 indicates identity
Score > 26 indicates homology
EIVFSGQLQE + Deamidated (NQ)
1665 633.8361 1265.6576 1265.6503 5.81 1 15 0.29 +1Score > 40 indicates identity
Score > 22 indicates homology
ITYTLIGENNK + Deamidated (NQ)
1994 476.2631 1425.7675 1425.7650 1.77 0 15 0.16 +1Score > 39 indicates identity
Score > 19 indicates homology
ALVLRPNGKPMTE + Deamidated (NQ)
1625 415.5680 1243.6822 1243.6884 -5.03 1 15 0.81 +1Score > 40 indicates identity
Score > 27 indicates homology
ALRATTQDQLK
3997 881.4344 2641.2814 2641.2945 -4.97 1 15 0.12 +1Score > 38 indicates identity
Score > 18 indicates homology
DAMINAVLLGQQFNALLPQNEVPE + 2 Deamidated (NQ); Oxidation (M)
2019 720.8796 1439.7446 1439.7521 -5.18 1 15 0.79 +1Score > 40 indicates identity
Score > 26 indicates homology
GVFNTRLSYVQR + Deamidated (NQ)
3093 671.3507 2011.0303 2011.0262 2.03 1 15 0.36 +1Score > 39 indicates identity
Score > 23 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
4702 747.5993 3732.9601 3732.9985 -10.3 1 15 0.088 +1Score > 32 indicates identity
Score > 17 indicates homology
VGTDGVLLGAWASVQGAHRILDIGTGTGLVALMLAQR + Deamidated (NQ); Oxidation (M)
239 308.1959 614.3772 614.3752 3.41 0 15 0.3 +1Score > 42 indicates identity
Score > 22 indicates homology
QGVIAK
435 358.2156 714.4166 714.4024 19.9 1 15 0.48 +1Score > 44 indicates identity
Score > 24 indicates homology
EGLAAVR
3097 671.3542 2011.0408 2011.0262 7.25 1 15 0.52 +1Score > 39 indicates identity
Score > 25 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
1462 586.3317 1170.6488 1170.6357 11.3 1 15 0.25 +1Score > 40 indicates identity
Score > 22 indicates homology
TLANSLPNGKR + Deamidated (NQ)
2572 849.9767 1697.9388 1697.9312 4.51 0 15 0.59 +1Score > 37 indicates identity
Score > 25 indicates homology
QLTQQGLGILGGSLADK
1533 404.2052 1209.5938 1209.5989 -4.26 1 15 0.68 +1Score > 41 indicates identity
Score > 26 indicates homology
QASAASKYQQK + Deamidated (NQ)
291 321.2036 640.3926 640.3908 2.87 0 15 0.93 +1Score > 35 indicates identity
Score > 27 indicates homology
AVGPLGK
1497 594.8261 1187.6376 1187.6186 16.0 1 15 0.73 +1Score > 41 indicates identity
Score > 26 indicates homology
WNKAINLISE + Deamidated (NQ)
2345 792.9402 1583.8658 1583.8519 8.83 1 15 0.53 +1Score > 38 indicates identity
Score > 25 indicates homology
NEIQVLNVVTADLR + Deamidated (NQ)
1192 519.2742 1036.5338 1036.5189 14.4 0 15 0.44 +1Score > 40 indicates identity
Score > 24 indicates homology
IAQVSQYAR + 2 Deamidated (NQ)
2479 825.9271 1649.8396 1649.8624 -13.8 0 15 0.35 +1Score > 40 indicates identity
Score > 23 indicates homology
ITTVQAAIDFIQSSR + Deamidated (NQ)
2189 761.4135 1520.8124 1520.8086 2.51 0 15 0.74 +1Score > 39 indicates identity
Score > 26 indicates homology
QVAVVQYPTLSTSK + Deamidated (NQ)
1933 700.3318 1398.6490 1398.6514 -1.69 0 15 0.4 +1Score > 40 indicates identity
Score > 23 indicates homology
IIHQITDLNNSE + 3 Deamidated (NQ)
2845 615.3268 1842.9586 1842.9761 -9.50 1 15 0.94 +1Score > 39 indicates identity
Score > 27 indicates homology
GLTMLDLVQEGNLGLIR + 2 Deamidated (NQ)
1821 671.3770 1340.7394 1340.7551 -11.7 0 15 0.71 +1Score > 38 indicates identity
Score > 26 indicates homology
LNPSLVISLSQGL + Deamidated (NQ)
2341 528.2687 1581.7843 1581.8038 -12.4 1 15 0.27 +1Score > 41 indicates identity
Score > 22 indicates homology
AGPLPPLNQEAAFQK + 2 Deamidated (NQ)
906 461.7316 921.4486 921.4443 4.68 1 15 0.39 +1Score > 42 indicates identity
Score > 23 indicates homology
LQEFQQK + 2 Deamidated (NQ)
3288 1064.5620 2127.1094 2127.0749 16.2 1 15 0.49 +1Score > 38 indicates identity
Score > 24 indicates homology
IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
1429 580.3586 1158.7026 1158.7046 -1.68 1 15 0.22 +1Score > 31 indicates identity
Score > 21 indicates homology
SLPLLMITKK + Oxidation (M)
3172 687.0258 2058.0556 2058.0278 13.5 1 15 0.5 +1Score > 39 indicates identity
Score > 24 indicates homology
VMFNTAFIRSNILMLNR + 3 Deamidated (NQ); Oxidation (M)
3610 769.0341 2304.0805 2304.0586 9.48 1 15 0.41 +1Score > 38 indicates identity
Score > 23 indicates homology
NKIFQNFFPDLFSSLQNDE + 2 Deamidated (NQ)
446 361.2173 720.4200 720.4204 -0.46 1 15 0.54 +1Score > 38 indicates identity
Score > 25 indicates homology
MSKIVK + Oxidation (M)
1744 435.8965 1304.6677 1304.6468 16.0 0 15 0.46 +1Score > 41 indicates identity
Score > 24 indicates homology
LNALMTLQCPK + Deamidated (NQ); Oxidation (M)
1185 518.2501 1034.4856 1034.4815 4.02 1 15 0.97 +1Score > 41 indicates identity
Score > 27 indicates homology
CTADDLKGR
1707 644.3708 1286.7270 1286.7194 5.94 1 15 0.85 +1Score > 38 indicates identity
Score > 27 indicates homology
DRVAASVDLITK
2826 365.7979 1823.9531 1823.9460 3.90 1 15 0.26 +1Score > 39 indicates identity
Score > 21 indicates homology
FMKVDVLGLGMLGCLR + Oxidation (M)
1789 662.8445 1323.6744 1323.6935 -14.4 0 15 0.34 +1Score > 41 indicates identity
Score > 23 indicates homology
LPGNPNAVWTVR + Deamidated (NQ)
2608 856.4557 1710.8968 1710.8689 16.3 1 15 0.48 +1Score > 39 indicates identity
Score > 24 indicates homology
NPLNGVVAWDDAVKGR + Deamidated (NQ)
1756 656.3726 1310.7306 1310.7446 -10.6 1 15 0.96 +1Score > 38 indicates identity
Score > 27 indicates homology
LLGPNIVDLKTE
614 398.2043 794.3940 794.3922 2.27 1 15 0.17 +1Score > 41 indicates identity
Score > 20 indicates homology
LSQEYR
1408 576.3643 1150.7140 1150.7298 -13.7 1 15 0.25 +1Score > 29 indicates identity
Score > 21 indicates homology
AIIARKPNLR
1492 593.3295 1184.6444 1184.6401 3.70 1 15 0.91 +1Score > 40 indicates identity
Score > 27 indicates homology
IQELINAVQR + 2 Deamidated (NQ)
2104 740.3552 1478.6958 1478.7226 -18.1 0 15 0.57 +1Score > 41 indicates identity
Score > 25 indicates homology
AQQIVSTPGHNGNR + Deamidated (NQ)
1485 394.8890 1181.6452 1181.6629 -15.0 1 15 0.57 +1Score > 38 indicates identity
Score > 25 indicates homology
NPRNLAAGTIR
969 316.4920 946.4542 946.4355 19.7 1 15 0.54 +1Score > 42 indicates identity
Score > 24 indicates homology
NVAESAQAR + 2 Deamidated (NQ)
2352 529.6339 1585.8799 1585.8981 -11.5 0 15 0.94 +1Score > 37 indicates identity
Score > 27 indicates homology
IGVVFNIPQPFTVR
383 345.6884 689.3622 689.3530 13.4 0 15 0.63 +1Score > 43 indicates identity
Score > 25 indicates homology
VMDAVR
853 451.2344 900.4542 900.4665 -13.6 0 15 0.86 +1Score > 41 indicates identity
Score > 26 indicates homology
VIGDQGGVR + Deamidated (NQ)
1410 385.2409 1152.7009 1152.6866 12.4 1 15 0.71 +1Score > 33 indicates identity
Score > 26 indicates homology
AKGLNPALLQK + Deamidated (NQ)
2893 938.4532 1874.8918 1874.9110 -10.2 1 15 0.81 +1Score > 40 indicates identity
Score > 26 indicates homology
QIEVTTDGAGVSGTVVDVE
1871 457.2232 1368.6478 1368.6707 -16.8 1 15 0.54 +1Score > 41 indicates identity
Score > 24 indicates homology
AHGEMLALAGAIAE + Oxidation (M)
3096 671.3535 2011.0387 2011.0262 6.20 1 15 0.62 +1Score > 39 indicates identity
Score > 25 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
277 318.1806 634.3466 634.3438 4.42 1 14 0.7 +1Score > 41 indicates identity
Score > 25 indicates homology
FEAIR
2995 972.0267 1942.0388 1942.0346 2.18 0 14 0.19 +1Score > 37 indicates identity
Score > 20 indicates homology
VFNMNKPGALLNIVVGGAE
438 358.7018 715.3890 715.3864 3.64 1 14 0.55 +1Score > 44 indicates identity
Score > 24 indicates homology
GLKDAVN
2842 614.6725 1840.9957 1840.9604 19.1 1 14 0.16 +1Score > 37 indicates identity
Score > 19 indicates homology
TVGLNVPNMAKIGLGNIE + 2 Deamidated (NQ)
3175 1030.5294 2059.0442 2059.0222 10.7 1 14 0.89 +1Score > 39 indicates identity
Score > 26 indicates homology
QTAQLSQDAKLSANWVLGE + Deamidated (NQ)
564 388.1921 774.3696 774.3759 -8.09 1 14 0.69 +1Score > 42 indicates identity
Score > 25 indicates homology
IEDAQAK + Deamidated (NQ)
2850 923.4609 1844.9072 1844.8839 12.6 1 14 0.26 +1Score > 40 indicates identity
Score > 21 indicates homology
SHVTLPQIRGMFNGDR + 2 Deamidated (NQ); Oxidation (M)
1884 458.2287 1371.6643 1371.6526 8.48 1 14 0.58 +1Score > 40 indicates identity
Score > 25 indicates homology
MMYKDVTQALR + Deamidated (NQ); Oxidation (M)
1993 476.2303 1425.6691 1425.6810 -8.33 1 14 0.45 +1Score > 40 indicates identity
Score > 23 indicates homology
QMTEGYIDLLSR + Deamidated (NQ)
795 439.2633 876.5120 876.5102 2.06 1 14 0.42 +1Score > 38 indicates identity
Score > 23 indicates homology
ASMISKLK
1496 594.8204 1187.6262 1187.6397 -11.3 1 14 0.33 +1Score > 42 indicates identity
Score > 22 indicates homology
LAEALTISQNK + Deamidated (NQ)
2445 544.6376 1630.8910 1630.9116 -12.7 1 14 0.26 +1Score > 38 indicates identity
Score > 21 indicates homology
LFGLPMAVKDNLSVK
576 390.2289 778.4432 778.4337 12.2 1 14 0.49 +1Score > 37 indicates identity
Score > 24 indicates homology
NKATAFK
2455 817.9307 1633.8468 1633.8715 -15.1 0 14 0.54 +1Score > 40 indicates identity
Score > 24 indicates homology
DLLVSYDIAIPPYR
858 301.5164 901.5274 901.5345 -7.91 1 14 0.96 +1Score > 39 indicates identity
Score > 27 indicates homology
VISNSKVR
1474 589.2956 1176.5766 1176.5986 -18.7 1 14 0.65 +1Score > 41 indicates identity
Score > 25 indicates homology
AITVDIESSSR
693 419.2495 836.4844 836.4909 -7.67 0 14 0.66 +1Score > 36 indicates identity
Score > 25 indicates homology
VPHPLFK
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