MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
776 435.2446 868.4746 868.4694 6.00 0 17 0.34 +1Score > 38 indicates identity
Score > 24 indicates homology
IYQFIGK + Deamidated (NQ)
1917 695.8804 1389.7462 1389.7503 -2.94 1 17 0.57 +1Score > 40 indicates identity
Score > 27 indicates homology
YIAKNIVAADLAE
3388 725.3666 2173.0780 2173.0611 7.78 1 17 0.31 +1Score > 39 indicates identity
Score > 24 indicates homology
QNTRPVSEVNASLDLNTASR + 2 Deamidated (NQ)
881 457.7727 913.5308 913.5233 8.30 1 17 0.38 +1Score > 41 indicates identity
Score > 25 indicates homology
QNKTVVPK + Deamidated (NQ)
3766 605.3205 2417.2529 2417.2660 -5.41 0 17 0.081 +1Score > 37 indicates identity
Score > 18 indicates homology
LSAMMVTSFILISVLALGLMYE + Oxidation (M)
1327 560.7922 1119.5698 1119.5772 -6.55 1 17 0.75 +1Score > 41 indicates identity
Score > 28 indicates homology
SSVTVSVKGQE
1662 632.8317 1263.6488 1263.6241 19.5 0 17 0.62 +1Score > 41 indicates identity
Score > 27 indicates homology
SSNTVVQITCR
1738 651.8568 1301.6990 1301.7191 -15.4 1 17 0.77 +1Score > 40 indicates identity
Score > 28 indicates homology
AVQDAIISTKQK + Deamidated (NQ)
486 372.2257 742.4368 742.4337 4.20 0 17 0.84 +1Score > 42 indicates identity
Score > 28 indicates homology
VVAQAVR + Deamidated (NQ)
1530 604.3264 1206.6382 1206.6244 11.5 0 16 0.95 +1Score > 41 indicates identity
Score > 29 indicates homology
IAQHPISLQAE + Deamidated (NQ)
1525 602.8416 1203.6686 1203.6724 -3.11 1 16 0.76 +1Score > 39 indicates identity
Score > 28 indicates homology
LITRAFGNQGK
2121 747.8843 1493.7540 1493.7435 7.03 1 16 0.41 +1Score > 41 indicates identity
Score > 25 indicates homology
YIDNKITMAIPAE + Oxidation (M)
243 308.6877 615.3608 615.3704 -15.5 0 16 0.96 +1Score > 45 indicates identity
Score > 29 indicates homology
AISAVR
2841 613.9763 1838.9071 1838.9414 -18.7 0 16 0.5 +1Score > 40 indicates identity
Score > 26 indicates homology
GLPADTFLNVNVPDLPR + 2 Deamidated (NQ)
950 470.3025 938.5904 938.5913 -0.88 1 16 0.56 +1Score > 32 indicates identity
Score > 26 indicates homology
LVLNPRVK + Deamidated (NQ)
287 319.1700 636.3254 636.3231 3.66 0 16 0.14 +1Score > 41 indicates identity
Score > 20 indicates homology
ISDFR
2670 871.9612 1741.9078 1741.8999 4.59 1 16 0.38 +1Score > 39 indicates identity
Score > 25 indicates homology
KLVNNADIYHDAIVR + 2 Deamidated (NQ)
1683 638.8483 1275.6820 1275.6935 -9.01 0 16 0.42 +1Score > 41 indicates identity
Score > 25 indicates homology
SVLFAAVGTAGQR
2241 515.6041 1543.7905 1543.8028 -7.99 1 16 0.85 +1Score > 41 indicates identity
Score > 28 indicates homology
VEQVLASQCLLQR + Deamidated (NQ)
1139 339.4993 1015.4761 1015.4934 -17.1 1 16 0.87 +1Score > 40 indicates identity
Score > 28 indicates homology
QVEDALQGR + Deamidated (NQ)
303 324.1680 646.3214 646.3187 4.26 0 16 0.78 +1Score > 44 indicates identity
Score > 28 indicates homology
SWQAR
2709 586.9617 1757.8633 1757.8360 15.5 0 16 0.21 +1Score > 40 indicates identity
Score > 22 indicates homology
DTFYQTALDDAISLGK + Deamidated (NQ)
3153 682.3588 2044.0546 2044.0701 -7.61 1 16 0.29 +1Score > 39 indicates identity
Score > 23 indicates homology
SDFGLLRSPLTPSNASINR
1171 343.5323 1027.5751 1027.5662 8.64 0 16 0.53 +1Score > 40 indicates identity
Score > 26 indicates homology
SQLSPQIVR + Deamidated (NQ)
3537 754.0679 2259.1819 2259.1575 10.8 1 16 0.15 +1Score > 37 indicates identity
Score > 20 indicates homology
NLLSFLRGTFLNNYVPFDK + 2 Deamidated (NQ)
1848 452.9254 1355.7544 1355.7296 18.3 1 16 0.44 +1Score > 39 indicates identity
Score > 25 indicates homology
ARLLQVLQQVGE + 3 Deamidated (NQ)
773 434.2941 866.5736 866.5701 4.04 0 16 0.63 +1Score > 28 indicates identity
Score > 27 indicates homology
LGLKPLAR
1731 649.8433 1297.6720 1297.6738 -1.37 1 16 0.47 +1Score > 40 indicates identity
Score > 25 indicates homology
NVTPNNNIRQK + Deamidated (NQ)
3106 1010.5099 2019.0052 2019.0056 -0.20 1 16 0.66 +1Score > 40 indicates identity
Score > 27 indicates homology
LESIVMNWLGQMLNLPK + 2 Deamidated (NQ); 2 Oxidation (M)
462 365.6976 729.3806 729.3770 5.07 0 16 0.4 +1Score > 41 indicates identity
Score > 25 indicates homology
VGGIDNR
2342 791.9014 1581.7882 1581.7998 -7.33 1 16 0.28 +1Score > 41 indicates identity
Score > 23 indicates homology
HVDDILESLQGLSR + Deamidated (NQ)
1971 708.8954 1415.7762 1415.7620 10.0 1 16 0.33 +1Score > 39 indicates identity
Score > 24 indicates homology
DVGVTVKTLDDVR
2211 765.4366 1528.8586 1528.8712 -8.21 1 16 0.68 +1Score > 37 indicates identity
Score > 27 indicates homology
LQTAITLIDELTAK
1456 586.2852 1170.5558 1170.5418 12.0 0 16 0.39 +1Score > 40 indicates identity
Score > 25 indicates homology
DGGPAIYGHQR + Deamidated (NQ)
3120 677.3900 2029.1482 2029.1248 11.5 1 16 0.27 +1Score > 32 indicates identity
Score > 23 indicates homology
FIDVLNLDFILIENVPR
473 367.7153 733.4160 733.4123 5.16 0 16 0.45 +1Score > 39 indicates identity
Score > 25 indicates homology
FADILR
2238 772.4305 1542.8464 1542.8439 1.64 0 16 0.38 +1Score > 38 indicates identity
Score > 24 indicates homology
NMVVSNILLNNIAK + Deamidated (NQ)
430 714.4028 713.3955 713.3960 -0.62 0 16 0.71 +1Score > 39 indicates identity
Score > 27 indicates homology
TVGIPVE
2047 485.9584 1454.8534 1454.8708 -12.0 1 16 0.81 +1Score > 34 indicates identity
Score > 28 indicates homology
LLDTKLAALQIQK + Deamidated (NQ)
1109 504.7555 1007.4964 1007.4811 15.2 0 16 0.32 +1Score > 42 indicates identity
Score > 24 indicates homology
IADYVQGIE + Deamidated (NQ)
2140 751.4063 1500.7980 1500.7797 12.2 0 16 0.93 +1Score > 40 indicates identity
Score > 28 indicates homology
GGGGTDLGPPAPPRPR
1081 332.8552 995.5438 995.5400 3.80 1 16 0.76 +1Score > 37 indicates identity
Score > 27 indicates homology
TVNVVEAHK
1470 391.8825 1172.6257 1172.6037 18.7 0 16 0.49 +1Score > 41 indicates identity
Score > 25 indicates homology
AGDVLVLNDTR + Deamidated (NQ)
2265 519.2999 1554.8779 1554.8478 19.4 1 16 0.47 +1Score > 36 indicates identity
Score > 25 indicates homology
ALVSAQLAPSRSQAR + Deamidated (NQ)
2151 753.3795 1504.7444 1504.7144 20.0 0 16 0.22 +1Score > 41 indicates identity
Score > 22 indicates homology
SINSSNTINIPITE + 3 Deamidated (NQ)
1339 562.2764 1122.5382 1122.5478 -8.53 0 16 0.11 +1Score > 41 indicates identity
Score > 19 indicates homology
LMIQSIVSSE + Deamidated (NQ); Oxidation (M)
286 637.3327 636.3254 636.3231 3.64 0 16 0.51 +1Score > 41 indicates identity
Score > 26 indicates homology
YIADR
808 442.2680 882.5214 882.5287 -8.20 0 16 0.73 +1Score > 36 indicates identity
Score > 27 indicates homology
LNTPIIGR
2354 794.4089 1586.8032 1586.7940 5.81 1 16 0.57 +1Score > 41 indicates identity
Score > 26 indicates homology
NYNDVVKTPAPAGLE
2606 570.6516 1708.9330 1708.9182 8.66 1 16 0.058 +1Score > 37 indicates identity
Score > 16 indicates homology
VTNYMALDGLLSKLR + Oxidation (M)
999 319.8681 956.5825 956.5807 1.83 1 16 0.48 +1Score > 38 indicates identity
Score > 25 indicates homology
HGIKLYVK
343 336.7124 671.4102 671.4078 3.59 1 16 0.61 +1Score > 42 indicates identity
Score > 26 indicates homology
AGGKALR
2744 592.3207 1773.9403 1773.9665 -14.8 1 16 0.1 +1Score > 39 indicates identity
Score > 19 indicates homology
LNLVVQDFIFKHTAK + 2 Deamidated (NQ)
2320 788.9147 1575.8148 1575.8257 -6.87 1 16 0.22 +1Score > 40 indicates identity
Score > 22 indicates homology
ETALFGGISGAVDLAR
2130 748.9009 1495.7872 1495.8021 -9.94 1 16 0.93 +1Score > 39 indicates identity
Score > 28 indicates homology
IPIELLQVNDIVE + 2 Deamidated (NQ)
1577 410.2086 1227.6040 1227.5996 3.53 0 16 0.43 +1Score > 40 indicates identity
Score > 25 indicates homology
QVQFDIQGHR + Deamidated (NQ)
721 423.7285 845.4424 845.4317 12.7 1 16 0.98 +1Score > 42 indicates identity
Score > 28 indicates homology
IPDDKMK
1085 499.8002 997.5858 997.5808 5.10 0 16 0.97 +1Score > 38 indicates identity
Score > 28 indicates homology
VSAIQPAIAK + Deamidated (NQ)
2365 797.4007 1592.7868 1592.8080 -13.3 1 16 0.88 +1Score > 41 indicates identity
Score > 28 indicates homology
LSKSNISMIGPTGSGK + Deamidated (NQ); Oxidation (M)
2614 571.9818 1712.9236 1712.9131 6.10 1 16 0.47 +1Score > 38 indicates identity
Score > 25 indicates homology
MIVLTGHTLTIDEVR + Oxidation (M)
1381 380.5540 1138.6402 1138.6460 -5.14 1 16 0.27 +1Score > 36 indicates identity
Score > 23 indicates homology
KFVFLMIIE
2289 523.6231 1567.8475 1567.8570 -6.05 0 16 0.3 +1Score > 38 indicates identity
Score > 23 indicates homology
AIIQPGGSINDGVSIK
1099 502.7754 1003.5362 1003.5338 2.41 0 16 0.57 +1Score > 41 indicates identity
Score > 26 indicates homology
YVIGQEPAK
1826 672.3572 1342.6998 1342.7108 -8.12 1 16 0.99 +1Score > 41 indicates identity
Score > 28 indicates homology
IFQFWKMISK + Oxidation (M)
1850 679.3246 1356.6346 1356.6534 -13.8 1 16 0.99 +1Score > 40 indicates identity
Score > 28 indicates homology
NPDNATWNRIR + Deamidated (NQ)
1823 671.3795 1340.7444 1340.7551 -7.95 0 16 0.38 +1Score > 39 indicates identity
Score > 24 indicates homology
LNPSLVISLSQGL + Deamidated (NQ)
2783 601.6655 1801.9747 1801.9825 -4.36 1 16 0.65 +1Score > 38 indicates identity
Score > 26 indicates homology
NIVNLNSGQLFEILVK + 2 Deamidated (NQ)
2194 381.4390 1521.7269 1521.7198 4.64 0 16 0.51 +1Score > 41 indicates identity
Score > 25 indicates homology
SQQLFNSLGNLGIE + 3 Deamidated (NQ)
2708 586.6514 1756.9324 1756.9393 -3.93 0 16 0.37 +1Score > 39 indicates identity
Score > 24 indicates homology
QLAQAMQALLPSLTVR + 2 Deamidated (NQ); Oxidation (M)
403 351.2328 700.4510 700.4483 3.92 0 16 0.39 +1Score > 38 indicates identity
Score > 24 indicates homology
LSGIIAK
728 425.7195 849.4244 849.4232 1.47 1 16 0.62 +1Score > 42 indicates identity
Score > 26 indicates homology
FLEQNAK + Deamidated (NQ)
1675 424.5707 1270.6903 1270.6670 18.3 1 16 0.38 +1Score > 40 indicates identity
Score > 24 indicates homology
ALEQPPYGVAAR
1620 621.8223 1241.6300 1241.6114 15.0 1 16 0.64 +1Score > 40 indicates identity
Score > 26 indicates homology
YGANMLIFGEK
1782 661.7993 1321.5840 1321.5898 -4.37 0 16 0.74 +1Score > 39 indicates identity
Score > 27 indicates homology
NSNGDIFVNNAR + 2 Deamidated (NQ)
1973 472.9476 1415.8210 1415.8024 13.1 1 16 0.98 +1Score > 34 indicates identity
Score > 28 indicates homology
LYIKGNGVVDLPK + Deamidated (NQ)
2138 750.4471 1498.8796 1498.8541 17.0 1 16 0.51 +1Score > 32 indicates identity
Score > 25 indicates homology
NKLLIIDGMNLVR + Deamidated (NQ)
4459 1042.5037 3124.4893 3124.5160 -8.55 1 16 0.056 +1Score > 36 indicates identity
Score > 16 indicates homology
TNSTTTIINNNTQNNFTNITNIIVSKNE + 2 Deamidated (NQ)
2225 769.3880 1536.7614 1536.7460 10.1 1 16 0.76 +1Score > 41 indicates identity
Score > 27 indicates homology
YLEVSDIVQQWR + 2 Deamidated (NQ)
1504 598.8383 1195.6620 1195.6448 14.4 1 16 0.81 +1Score > 38 indicates identity
Score > 27 indicates homology
KSITAQYLSGK + Deamidated (NQ)
2418 808.4308 1614.8470 1614.8729 -16.0 1 16 0.35 +1Score > 40 indicates identity
Score > 23 indicates homology
RNFLNQAASLLLPR + 3 Deamidated (NQ)
1857 455.2218 1362.6436 1362.6627 -14.0 0 15 0.89 +1Score > 41 indicates identity
Score > 28 indicates homology
TGPSSSSASLPSTGK
2035 725.3731 1448.7316 1448.7147 11.7 0 15 0.66 +1Score > 41 indicates identity
Score > 26 indicates homology
VLNQFDDAGIVTR + 2 Deamidated (NQ)
1977 711.3486 1420.6826 1420.7054 -16.0 1 15 0.92 +1Score > 41 indicates identity
Score > 28 indicates homology
INEAQLMQLMAK + 2 Oxidation (M)
2244 516.2690 1545.7852 1545.7787 4.19 1 15 0.43 +1Score > 41 indicates identity
Score > 24 indicates homology
GTPFAAQTAAENVIR + Deamidated (NQ)
2378 535.2700 1602.7882 1602.7638 15.2 0 15 0.26 +1Score > 41 indicates identity
Score > 22 indicates homology
NGYSPQDAVQASLPR + Deamidated (NQ)
2702 877.9653 1753.9160 1753.9144 0.91 1 15 0.61 +1Score > 39 indicates identity
Score > 26 indicates homology
NLPPNARNMSLQILR + 2 Deamidated (NQ); Oxidation (M)
3461 1105.1326 2208.2506 2208.2531 -1.11 1 15 0.47 +1Score > 31 indicates identity
Score > 25 indicates homology
NIINLEPVLNVLRWHLHK + Deamidated (NQ)
852 301.1581 900.4525 900.4665 -15.6 0 15 0.7 +1Score > 41 indicates identity
Score > 26 indicates homology
GAGLDNAVGK
477 368.7046 735.3946 735.3915 4.22 0 15 0.28 +1Score > 41 indicates identity
Score > 22 indicates homology
VNFTQK
1370 379.8747 1136.6023 1136.6012 0.96 1 15 0.72 +1Score > 40 indicates identity
Score > 27 indicates homology
KFMAALAAGNK + Oxidation (M)
1902 693.8905 1385.7664 1385.7402 19.0 1 15 0.63 +1Score > 39 indicates identity
Score > 26 indicates homology
IIIASDEQLDLR + Deamidated (NQ)
3272 707.6832 2120.0278 2120.0273 0.20 0 15 0.32 +1Score > 40 indicates identity
Score > 23 indicates homology
QVLTTPFVANDLSLDDNTR + 2 Deamidated (NQ)
1127 507.7913 1013.5680 1013.5505 17.3 1 15 0.66 +1Score > 41 indicates identity
Score > 26 indicates homology
QVAAELLDR
884 305.8167 914.4283 914.4385 -11.2 1 15 0.71 +1Score > 39 indicates identity
Score > 26 indicates homology
TFYNIEK + Deamidated (NQ)
2839 919.9526 1837.8906 1837.8767 7.57 1 15 0.15 +1Score > 40 indicates identity
Score > 20 indicates homology
GNEYTPQQISAMILQK + 2 Deamidated (NQ); Oxidation (M)
3104 1010.0752 2018.1358 2018.1008 17.4 1 15 0.12 +1Score > 33 indicates identity
Score > 19 indicates homology
ITKTANTITVSGTGNITGIR + Deamidated (NQ)
613 398.1992 794.3838 794.3810 3.57 1 15 0.72 +1Score > 41 indicates identity
Score > 26 indicates homology
AVFENSK + Deamidated (NQ)
820 444.7206 887.4266 887.4249 1.92 0 15 0.36 +1Score > 41 indicates identity
Score > 23 indicates homology
HNNSLFR + Deamidated (NQ)
2961 642.3190 1923.9352 1923.9361 -0.46 1 15 0.26 +1Score > 40 indicates identity
Score > 22 indicates homology
DGTVRYDMTDLPVTAVR + Oxidation (M)
156 501.3176 500.3103 500.3071 6.48 0 15 0.14 +1Score > 43 indicates identity
Score > 19 indicates homology
AVVGR
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