MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2044 485.6261 1453.8565 1453.8293 18.7 0 18 0.59 +1Score > 33 indicates identity
Score > 28 indicates homology		 SQLLNGLPPLVFR + Deamidated (NQ)
2927 951.0213 1900.0280 1899.9955 17.1 1 18 0.33 +1Score > 37 indicates identity
Score > 25 indicates homology		 TRWQLHQAQPLPSIPE
1668 633.8807 1265.7468 1265.7343 9.90 1 18 0.28 +1Score > 34 indicates identity
Score > 25 indicates homology		 LQLQELGPVIR + Deamidated (NQ)
372 344.1943 686.3740 686.3711 4.26 0 18 0.7 +1Score > 43 indicates identity
Score > 29 indicates homology		 VAQVNR + Deamidated (NQ)
1004 479.7856 957.5566 957.5495 7.50 1 18 0.51 +1Score > 40 indicates identity
Score > 27 indicates homology		 LSLRQLVE + Deamidated (NQ)
1686 426.5766 1276.7080 1276.7000 6.25 0 18 0.19 +1Score > 37 indicates identity
Score > 23 indicates homology		 IDIHRPAQTAR
561 387.2412 772.4678 772.4629 6.38 0 18 0.27 +1Score > 40 indicates identity
Score > 24 indicates homology		 CIVILR
1444 584.3156 1166.6166 1166.6084 7.06 0 18 0.51 +1Score > 39 indicates identity
Score > 27 indicates homology		 YFGVDALVAGR
2006 718.3651 1434.7156 1434.6991 11.6 1 18 0.39 +1Score > 41 indicates identity
Score > 26 indicates homology		 NKVDGVYTADPQK + Deamidated (NQ)
600 395.2076 788.4006 788.3963 5.53 0 18 0.17 +1Score > 44 indicates identity
Score > 22 indicates homology		 AVQNAMR
1230 531.7676 1061.5206 1061.5175 2.94 1 18 0.93 +1Score > 42 indicates identity
Score > 30 indicates homology		 NLMVEGDLR + Oxidation (M)
2747 889.4720 1776.9294 1776.9040 14.3 0 18 0.2 +1Score > 39 indicates identity
Score > 23 indicates homology		 DASLVAVSTGLLQNMSR + Oxidation (M)
2007 718.3746 1434.7346 1434.7354 -0.54 1 17 0.17 +1Score > 41 indicates identity
Score > 22 indicates homology		 LQNQVLDSKNFK + 2 Deamidated (NQ)
3190 688.0420 2061.1042 2061.0677 17.7 0 17 0.18 +1Score > 37 indicates identity
Score > 23 indicates homology		 NQTFIIVGAGQAGAMAAATLR + Deamidated (NQ)
993 319.8393 956.4961 956.5152 -20.0 1 17 0.85 +1Score > 40 indicates identity
Score > 29 indicates homology		 GRGIVNADR
2273 781.4025 1560.7904 1560.8035 -8.38 0 17 0.87 +1Score > 41 indicates identity
Score > 29 indicates homology		 LFILGGGSNILVNDE + Deamidated (NQ)
1286 548.7771 1095.5396 1095.5304 8.45 1 17 0.89 +1Score > 41 indicates identity
Score > 29 indicates homology		 MELLMNSVK + 2 Oxidation (M)
3103 673.7178 2018.1316 2018.1008 15.3 1 17 0.13 +1Score > 33 indicates identity
Score > 21 indicates homology		 ITKTANTITVSGTGNITGIR + Deamidated (NQ)
1830 672.8735 1343.7324 1343.7561 -17.6 0 17 0.93 +1Score > 40 indicates identity
Score > 30 indicates homology		 ANLAFVVSIASPR
998 479.2913 956.5680 956.5542 14.5 0 17 0.54 +1Score > 39 indicates identity
Score > 27 indicates homology		 IADAINVLK + Deamidated (NQ)
1063 497.2740 992.5334 992.5403 -6.90 1 17 0.88 +1Score > 40 indicates identity
Score > 29 indicates homology		 LHLPSEAAR
2704 585.9828 1754.9266 1754.9236 1.68 1 17 0.71 +1Score > 39 indicates identity
Score > 28 indicates homology		 NKTLDPLLMSEPKPR + Deamidated (NQ); Oxidation (M)
777 435.7521 869.4896 869.4970 -8.50 0 17 0.58 +1Score > 38 indicates identity
Score > 28 indicates homology		 KPAGADLAK
406 351.6939 701.3732 701.3820 -12.5 1 17 0.41 +1Score > 45 indicates identity
Score > 26 indicates homology		 IGKDNR
535 381.7305 761.4464 761.4469 -0.63 1 17 0.81 +1Score > 38 indicates identity
Score > 29 indicates homology		 MGALKVK + Oxidation (M)
818 444.2689 886.5232 886.5236 -0.40 0 17 0.64 +1Score > 40 indicates identity
Score > 28 indicates homology		 ITGILSQR
1130 508.2759 1014.5372 1014.5233 13.7 1 17 0.98 +1Score > 41 indicates identity
Score > 30 indicates homology		 LAAELLDAAE
1914 695.8281 1389.6416 1389.6372 3.21 1 17 0.71 +1Score > 40 indicates identity
Score > 28 indicates homology		 ADSLTAGGLDARDE
3669 782.0950 2343.2632 2343.2845 -9.10 1 17 0.076 +1Score > 35 indicates identity
Score > 19 indicates homology		 MQAQHLLQIQPIPGVGRTALR + Deamidated (NQ); Oxidation (M)
1387 381.5537 1141.6393 1141.6343 4.38 0 17 0.94 +1Score > 40 indicates identity
Score > 30 indicates homology		 LNGPTDIALTK
456 365.1993 728.3840 728.3817 3.22 0 17 0.72 +1Score > 39 indicates identity
Score > 28 indicates homology		 TGDPLAR
731 426.2124 850.4102 850.4072 3.55 1 17 0.78 +1Score > 42 indicates identity
Score > 29 indicates homology		 QLEFADK + Deamidated (NQ)
3176 687.3555 2059.0447 2059.0143 14.7 1 17 0.47 +1Score > 39 indicates identity
Score > 27 indicates homology		 TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
2388 537.3425 1609.0057 1608.9814 15.1 1 17 0.14 +1Score > 21 indicates identity LSPAEVALITTLLLR
985 317.8361 950.4865 950.4862 0.33 1 17 0.88 +1Score > 41 indicates identity
Score > 29 indicates homology		 AEWFTIGK
1356 566.2853 1130.5560 1130.5642 -7.18 0 17 0.93 +1Score > 41 indicates identity
Score > 29 indicates homology		 VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
1702 429.5513 1285.6321 1285.6336 -1.22 0 17 0.66 +1Score > 40 indicates identity
Score > 28 indicates homology		 GTVILDMAHGQK + Deamidated (NQ); Oxidation (M)
1351 565.7782 1129.5418 1129.5516 -8.64 1 17 0.4 +1Score > 40 indicates identity
Score > 26 indicates homology		 SPQWVRDNK + Deamidated (NQ)
404 351.6937 701.3728 701.3708 2.94 0 17 0.69 +1Score > 45 indicates identity
Score > 28 indicates homology		 VVNNVR + 2 Deamidated (NQ)
983 475.7736 949.5326 949.5233 9.88 0 17 0.41 +1Score > 37 indicates identity
Score > 26 indicates homology		 ISFNTVLR + Deamidated (NQ)
3177 1030.5336 2059.0526 2059.0884 -17.4 1 17 0.27 +1Score > 39 indicates identity
Score > 24 indicates homology		 ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
1206 524.7782 1047.5418 1047.5237 17.3 0 17 0.6 +1Score > 41 indicates identity
Score > 27 indicates homology		 IGSGFDLPDK
1221 353.5284 1057.5634 1057.5768 -12.7 1 17 0.35 +1Score > 42 indicates identity
Score > 25 indicates homology		 NTVDVEILR
469 366.7073 731.4000 731.4038 -5.16 1 17 0.53 +1Score > 41 indicates identity
Score > 27 indicates homology		 VGSRASR
416 352.2228 702.4310 702.4276 4.93 0 17 0.37 +1Score > 38 indicates identity
Score > 25 indicates homology		 VAATTIK
244 308.6881 615.3616 615.3704 -14.2 1 17 0.83 +1Score > 45 indicates identity
Score > 29 indicates homology		 AAAKGAK
1209 350.8747 1049.6023 1049.5982 3.92 1 17 0.87 +1Score > 36 indicates identity
Score > 29 indicates homology		 APHQTIVKR + Deamidated (NQ)
1957 705.3969 1408.7792 1408.8038 -17.4 1 17 0.55 +1Score > 37 indicates identity
Score > 27 indicates homology		 QIVKITQAGPTPR + Deamidated (NQ)
956 470.7973 939.5800 939.5865 -6.90 1 17 0.89 +1Score > 35 indicates identity
Score > 29 indicates homology		 IVRTLNPK
1983 474.6075 1420.8007 1420.7786 15.5 1 17 0.35 +1Score > 36 indicates identity
Score > 25 indicates homology		 ARIGPPINTQPTR + Deamidated (NQ)
2096 492.6427 1474.9063 1474.8984 5.35 0 17 0.38 +1Score > 25 indicates identity ITIHTARPGIVIGK
1931 700.3299 1398.6452 1398.6490 -2.65 1 17 0.85 +1Score > 40 indicates identity
Score > 29 indicates homology		 WYVIKGGMDVSQ + Deamidated (NQ); Oxidation (M)
1888 687.8895 1373.7644 1373.7554 6.55 1 17 0.54 +1Score > 38 indicates identity
Score > 27 indicates homology		 LFQLLDGQLAEK
2331 527.5970 1579.7692 1579.7664 1.72 1 17 0.46 +1Score > 41 indicates identity
Score > 26 indicates homology		 TWSMVSVLRQGATE + Oxidation (M)
479 369.1966 736.3786 736.3901 -15.6 1 17 0.64 +1Score > 42 indicates identity
Score > 27 indicates homology		 ISRMSK + Oxidation (M)
332 335.1912 668.3678 668.3646 4.89 0 17 0.85 +1Score > 34 indicates identity
Score > 29 indicates homology		 FYIAR
945 469.2419 936.4692 936.4665 2.94 1 17 0.48 +1Score > 41 indicates identity
Score > 26 indicates homology		 VISGEGYGR
1211 526.2475 1050.4804 1050.4764 3.86 1 17 0.5 +1Score > 41 indicates identity
Score > 26 indicates homology		 AISEMSGGQR + Oxidation (M)
703 421.2628 840.5110 840.5069 4.96 1 17 0.82 +1Score > 35 indicates identity
Score > 29 indicates homology		 QLKDIPK
1051 494.2661 986.5176 986.5033 14.5 0 17 0.49 +1Score > 42 indicates identity
Score > 26 indicates homology		 GGEPGVSGTVK
3376 724.3981 2170.1725 2170.1436 13.3 1 17 0.36 +1Score > 36 indicates identity
Score > 25 indicates homology		 LRHSSAAFLFGNLPDWIAR
2605 855.4578 1708.9010 1708.8672 19.8 1 17 0.33 +1Score > 39 indicates identity
Score > 25 indicates homology		 QLAGDIALELQAYFR + 2 Deamidated (NQ)
2578 567.9527 1700.8363 1700.8370 -0.40 0 17 0.096 +1Score > 40 indicates identity
Score > 19 indicates homology		 LGIDFQSDAQHVTIR + 2 Deamidated (NQ)
3075 667.0026 1997.9860 1997.9656 10.2 0 17 0.32 +1Score > 40 indicates identity
Score > 24 indicates homology		 LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
1291 549.8718 1097.7290 1097.7172 10.8 1 17 0.16 +1Score > 21 indicates identity LIAQLIKTAK
3076 667.0029 1997.9869 1997.9656 10.7 0 17 0.32 +1Score > 40 indicates identity
Score > 24 indicates homology		 LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
1833 673.3555 1344.6964 1344.6959 0.42 1 17 0.47 +1Score > 41 indicates identity
Score > 26 indicates homology		 AIDLLGSKTAPME
3171 687.0252 2058.0538 2058.0278 12.6 1 17 0.53 +1Score > 39 indicates identity
Score > 27 indicates homology		 VMFNTAFIRSNILMLNR + 3 Deamidated (NQ); Oxidation (M)
826 447.2432 892.4718 892.4767 -5.39 1 17 0.89 +1Score > 41 indicates identity
Score > 29 indicates homology		 DIFKNTR
2120 747.8835 1493.7524 1493.7435 5.96 1 17 0.99 +1Score > 41 indicates identity
Score > 29 indicates homology		 YIDNKITMAIPAE + Oxidation (M)
2875 466.0193 1860.0481 1860.0832 -18.9 1 17 0.52 +1Score > 33 indicates identity
Score > 26 indicates homology		 ARLPLAPLQPSSLQVLR + 2 Deamidated (NQ)
636 403.2322 804.4498 804.4606 -13.4 1 17 0.97 +1Score > 41 indicates identity
Score > 29 indicates homology		 FDVRLR
1292 550.3058 1098.5970 1098.5961 0.89 1 17 0.46 +1Score > 40 indicates identity
Score > 26 indicates homology		 LSEAIYLYK
230 304.1955 606.3764 606.3741 3.87 0 17 0.15 +1Score > 35 indicates identity
Score > 21 indicates homology		 VTFIK
269 630.4205 629.4132 629.4112 3.23 0 17 0.7 +1Score > 38 indicates identity
Score > 28 indicates homology		 AIVSIK
3180 1030.5355 2059.0564 2059.0321 11.8 1 17 0.41 +1Score > 39 indicates identity
Score > 25 indicates homology		 DTRISGDILLQAITQGINE + 3 Deamidated (NQ)
927 464.7800 927.5454 927.5389 7.06 1 17 0.59 +1Score > 40 indicates identity
Score > 27 indicates homology		 LNQELIAK
222 301.6983 601.3820 601.3799 3.55 0 17 0.79 +1Score > 41 indicates identity
Score > 28 indicates homology		 SVLVGK
566 388.2337 774.4528 774.4487 5.36 0 17 0.97 +1Score > 39 indicates identity
Score > 29 indicates homology		 ISLTVNK + Deamidated (NQ)
3169 1030.0300 2058.0454 2058.0051 19.6 1 17 0.32 +1Score > 39 indicates identity
Score > 24 indicates homology		 ALANAEVNGQAGGGLVQVTMR + 3 Deamidated (NQ)
3348 1077.5334 2153.0522 2153.0827 -14.1 0 17 0.36 +1Score > 39 indicates identity
Score > 25 indicates homology		 MVLYSDNLLTTIAWGLNGR + Deamidated (NQ); Oxidation (M)
466 366.2278 730.4410 730.4378 4.50 0 17 0.42 +1Score > 40 indicates identity
Score > 25 indicates homology		 VPPPPPK
2264 519.2995 1554.8767 1554.8478 18.6 1 17 0.7 +1Score > 36 indicates identity
Score > 28 indicates homology		 ALVSAQLAPSRSQAR + Deamidated (NQ)
539 382.2278 762.4410 762.4388 2.93 0 17 0.57 +1Score > 40 indicates identity
Score > 27 indicates homology		 LFSAVAR
622 400.7135 799.4124 799.4188 -7.92 1 17 0.38 +1Score > 38 indicates identity
Score > 25 indicates homology		 VNPRANK + 2 Deamidated (NQ)
3289 710.0443 2127.1111 2127.0960 7.07 1 17 0.66 +1Score > 38 indicates identity
Score > 27 indicates homology		 AADTAGDLAGLEIAGPGFINVR
1582 410.2285 1227.6637 1227.6823 -15.2 1 17 0.55 +1Score > 40 indicates identity
Score > 27 indicates homology		 QPDSQVTVKVK
1635 417.9057 1250.6953 1250.7095 -11.4 1 17 0.4 +1Score > 37 indicates identity
Score > 25 indicates homology		 KAGADGITIHLR
3077 667.0046 1997.9920 1997.9656 13.2 0 17 0.52 +1Score > 40 indicates identity
Score > 26 indicates homology		 LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
907 461.7548 921.4950 921.4920 3.35 1 17 0.26 +1Score > 40 indicates identity
Score > 23 indicates homology		 YLIETQR
1942 701.3977 1400.7808 1400.7664 10.3 1 17 0.59 +1Score > 38 indicates identity
Score > 27 indicates homology		 DVGWTANKIVLGK + Deamidated (NQ)
2142 751.9055 1501.7964 1501.8213 -16.5 1 17 0.53 +1Score > 40 indicates identity
Score > 26 indicates homology		 KIQTTATTQAVQGR
1119 506.3135 1010.6124 1010.6012 11.2 0 17 0.57 +1Score > 33 indicates identity
Score > 27 indicates homology		 INALQLIPK + 2 Deamidated (NQ)
2176 758.3661 1514.7176 1514.7293 -7.71 1 17 0.67 +1Score > 41 indicates identity
Score > 27 indicates homology		 VFSVFALFENDVE
2840 920.4573 1838.9000 1838.9163 -8.83 0 17 0.84 +1Score > 40 indicates identity
Score > 28 indicates homology		 QQVAAYGGGPVIVPDDPR + Deamidated (NQ)
774 435.2412 868.4678 868.4766 -10.1 0 17 0.94 +1Score > 38 indicates identity
Score > 29 indicates homology		 LPDNAAIR
1806 666.8453 1331.6760 1331.6768 -0.59 1 17 0.87 +1Score > 42 indicates identity
Score > 29 indicates homology		 CGLLPGFADARR
2124 748.3284 1494.6422 1494.6297 8.43 1 17 0.26 +1Score > 38 indicates identity
Score > 23 indicates homology		 AEPTEQSFMQPGR + 2 Deamidated (NQ); Oxidation (M)
3722 794.0999 2379.2779 2379.2885 -4.49 0 17 0.3 +1Score > 35 indicates identity
Score > 24 indicates homology		 FGIGADGVLFLAKPLHTSLHMR
686 417.2497 832.4848 832.4807 5.00 1 17 0.77 +1Score > 38 indicates identity
Score > 28 indicates homology		 IFGEVIR
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