MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1828 448.9175 1343.7307 1343.7483 -13.1 0 19 0.59 +1Score > 40 indicates identity
Score > 29 indicates homology		 GLQMSLDVAGLLK
1305 369.8680 1106.5822 1106.5641 16.3 1 19 0.076 +1Score > 41 indicates identity
Score > 21 indicates homology		 KMNSAVILSE + Oxidation (M)
2338 791.3873 1580.7600 1580.7769 -10.7 1 19 0.66 +1Score > 41 indicates identity
Score > 30 indicates homology		 QDIQKLFSTWCR
261 315.1858 628.3570 628.3544 4.19 0 19 0.71 +1Score > 37 indicates identity
Score > 30 indicates homology		 AVNTPK
2458 545.6293 1633.8661 1633.8536 7.65 1 19 0.98 +1Score > 39 indicates identity
Score > 32 indicates homology		 TGRSAQLLSQYRPR + 2 Deamidated (NQ)
2612 571.6663 1711.9771 1711.9608 9.53 1 19 0.14 +1Score > 34 indicates identity
Score > 23 indicates homology		 QQPITTTLELVDIIK + Deamidated (NQ)
1415 1155.5958 1154.5885 1154.6084 -17.2 1 19 0.37 +1Score > 40 indicates identity
Score > 27 indicates homology		 YNLAGFSAGKK
966 473.2497 944.4848 944.4814 3.60 1 19 0.69 +1Score > 42 indicates identity
Score > 30 indicates homology		 LDQLAAEGK + Deamidated (NQ)
1601 411.8962 1232.6668 1232.6652 1.26 0 19 0.76 +1Score > 40 indicates identity
Score > 30 indicates homology		 TLNLLFALAQE + Deamidated (NQ)
528 380.2540 758.4934 758.4902 4.32 0 19 0.66 +1Score > 30 indicates identity VISILSK
2866 619.6620 1855.9642 1855.9891 -13.4 0 19 0.66 +1Score > 39 indicates identity
Score > 30 indicates homology		 VLTSDSNIVPVDISGIAR + Deamidated (NQ)
965 472.7957 943.5768 943.5702 7.02 1 19 0.63 +1Score > 36 indicates identity
Score > 30 indicates homology		 TITVLIER
4056 900.4854 2698.4344 2698.4516 -6.40 0 19 0.16 +1Score > 35 indicates identity
Score > 23 indicates homology		 AVLQPLPPTSPALLITQHMPPGFTR + Deamidated (NQ); Oxidation (M)
1690 640.8522 1279.6898 1279.6996 -7.64 1 19 0.96 +1Score > 39 indicates identity
Score > 31 indicates homology		 QARINAHLSIR + 2 Deamidated (NQ)
1565 610.8362 1219.6578 1219.6561 1.46 0 19 0.48 +1Score > 40 indicates identity
Score > 28 indicates homology		 ISLGLFSNVNR + Deamidated (NQ)
912 925.4407 924.4334 924.4413 -8.54 1 19 0.61 +1Score > 40 indicates identity
Score > 29 indicates homology		 ASHPSAAER
3092 1006.4962 2010.9778 2010.9660 5.88 0 19 0.55 +1Score > 40 indicates identity
Score > 29 indicates homology		 QLTHQQIYPHFADGSNR
1331 560.8061 1119.5976 1119.5924 4.68 0 19 0.5 +1Score > 41 indicates identity
Score > 28 indicates homology		 GAVVAYANDLK
1856 682.3283 1362.6420 1362.6636 -15.8 0 19 0.53 +1Score > 41 indicates identity
Score > 29 indicates homology		 AMQSVMDAGAVGVK
3860 837.0757 2508.2053 2508.1703 13.9 0 19 0.77 +1Score > 38 indicates identity
Score > 30 indicates homology		 ATCTTHHDSPDADLITANLLWR + Deamidated (NQ)
2132 749.9357 1497.8568 1497.8515 3.58 1 19 0.74 +1Score > 35 indicates identity
Score > 30 indicates homology		 TVLKRPVDLTNDK
221 301.6865 601.3584 601.3588 -0.55 0 19 0.96 +1Score > 43 indicates identity
Score > 31 indicates homology		 WIVGK
2135 749.9392 1497.8638 1497.8515 8.25 1 19 0.74 +1Score > 35 indicates identity
Score > 30 indicates homology		 TVLKRPVDLTNDK
716 423.2499 844.4852 844.5018 -19.6 0 19 0.73 +1Score > 40 indicates identity
Score > 30 indicates homology		 IISLGAGSK
1619 621.4024 1240.7902 1240.8118 -17.4 1 19 0.2 +1Score > 25 indicates identity
Score > 24 indicates homology		 KISISLIGGLLK
1754 437.2231 1308.6475 1308.6561 -6.61 1 19 0.17 +1Score > 41 indicates identity
Score > 24 indicates homology		 QNITETSFVLR + 2 Deamidated (NQ)
1442 583.7873 1165.5600 1165.5417 15.7 1 19 0.47 +1Score > 41 indicates identity
Score > 28 indicates homology		 WFSDHRYR
1777 661.3375 1320.6604 1320.6786 -13.7 1 19 0.94 +1Score > 42 indicates identity
Score > 31 indicates homology		 DAFRAVTQSAVR + Deamidated (NQ)
2062 366.4662 1461.8357 1461.8113 16.7 1 19 0.067 +1Score > 35 indicates identity
Score > 19 indicates homology		 MTVLTQEAIITVK + Oxidation (M)
895 459.7425 917.4704 917.4818 -12.4 1 19 0.16 +1Score > 43 indicates identity
Score > 23 indicates homology		 IVLENSSR + Deamidated (NQ)
1515 601.3416 1200.6686 1200.6601 7.09 1 19 0.75 +1Score > 40 indicates identity
Score > 30 indicates homology		 DLVQQKDILK + 2 Deamidated (NQ)
2326 790.4491 1578.8836 1578.8729 6.79 1 19 0.38 +1Score > 36 indicates identity
Score > 27 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
2235 772.4037 1542.7928 1542.8140 -13.7 0 19 0.18 +1Score > 40 indicates identity
Score > 24 indicates homology		 LAIQNSQIASAIALE + 2 Deamidated (NQ)
2598 854.9482 1707.8818 1707.8679 8.17 1 19 0.74 +1Score > 40 indicates identity
Score > 30 indicates homology		 QLQLVKDVLNHAAQE + 3 Deamidated (NQ)
3178 687.3588 2059.0546 2059.0143 19.6 1 19 0.46 +1Score > 39 indicates identity
Score > 28 indicates homology		 TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
336 336.1565 670.2984 670.2922 9.31 0 19 0.33 +1Score > 37 indicates identity
Score > 26 indicates homology		 ASGPPNE
2261 777.4089 1552.8032 1552.8177 -9.31 1 19 0.23 +1Score > 40 indicates identity
Score > 25 indicates homology		 VFLLKYYPGINPE + Deamidated (NQ)
1808 667.3389 1332.6632 1332.6595 2.81 1 18 0.66 +1Score > 42 indicates identity
Score > 29 indicates homology		 LQKQVSQLVCE + 2 Deamidated (NQ)
586 394.2600 786.5054 786.5004 6.48 0 18 0.88 +1Score > 33 indicates identity
Score > 30 indicates homology		 APFVLIK
2012 718.9128 1435.8110 1435.7922 13.1 1 18 0.9 +1Score > 35 indicates identity
Score > 31 indicates homology		 AISKILDAISDYK
1583 615.3333 1228.6520 1228.6663 -11.6 1 18 0.61 +1Score > 41 indicates identity
Score > 29 indicates homology		 TGVSLQPETVAK
1793 663.4137 1324.8128 1324.8078 3.80 1 18 0.41 +1Score > 30 indicates identity
Score > 27 indicates homology		 NVVVVAANGKILK + Deamidated (NQ)
1253 539.7819 1077.5492 1077.5641 -13.8 1 18 0.69 +1Score > 42 indicates identity
Score > 29 indicates homology		 VLFNGRLME
1932 700.3311 1398.6476 1398.6490 -0.94 1 18 0.74 +1Score > 40 indicates identity
Score > 30 indicates homology		 WYVIKGGMDVSQ + Deamidated (NQ); Oxidation (M)
1982 474.5826 1420.7260 1420.7061 14.0 0 18 0.75 +1Score > 41 indicates identity
Score > 30 indicates homology		 QLQWMPFVNIK + 2 Deamidated (NQ); Oxidation (M)
3774 606.5732 2422.2637 2422.2605 1.33 1 18 0.35 +1Score > 37 indicates identity
Score > 26 indicates homology		 QSIIAAISPDKDVDGFHVINAGR
574 779.3759 778.3686 778.3709 -2.88 0 18 0.55 +1Score > 41 indicates identity
Score > 28 indicates homology		 ITDDSTK
489 372.7069 743.3992 743.4038 -6.16 1 18 0.91 +1Score > 41 indicates identity
Score > 30 indicates homology		 LGDGARR
1901 462.9198 1385.7376 1385.7337 2.82 1 18 0.4 +1Score > 40 indicates identity
Score > 27 indicates homology		 RVAIASVLAMNPE + Oxidation (M)
362 341.6754 681.3362 681.3333 4.29 0 18 0.73 +1Score > 38 indicates identity
Score > 29 indicates homology		 NAYSVK + Deamidated (NQ)
797 440.2522 878.4898 878.4974 -8.59 1 18 0.38 +1Score > 38 indicates identity
Score > 27 indicates homology		 AKFSTGIR
1608 619.3346 1236.6546 1236.6390 12.6 1 18 0.22 +1Score > 40 indicates identity
Score > 24 indicates homology		 GAEYVVDFLPK
1337 374.5508 1120.6306 1120.6240 5.83 1 18 0.49 +1Score > 38 indicates identity
Score > 28 indicates homology		 LLGSTSKYPR
3379 724.4376 2170.2910 2170.2911 -0.076 0 18 0.26 +1Score > 25 indicates identity LIIFAMNGVLVAIAGLILSAR + Oxidation (M)
236 307.1815 612.3484 612.3595 -18.0 0 18 0.17 +1Score > 38 indicates identity
Score > 23 indicates homology		 PNIIR + Deamidated (NQ)
302 323.7007 645.3868 645.3850 2.90 0 18 0.6 +1Score > 39 indicates identity
Score > 28 indicates homology		 AFLPAK
1949 468.9143 1403.7211 1403.6946 18.9 0 18 0.3 +1Score > 41 indicates identity
Score > 25 indicates homology		 NFHQHQITHLK + 2 Deamidated (NQ)
1563 610.3291 1218.6436 1218.6390 3.81 1 18 0.89 +1Score > 41 indicates identity
Score > 30 indicates homology		 AALASMQRLNK + Deamidated (NQ); Oxidation (M)
417 352.2228 702.4310 702.4276 4.93 0 18 0.99 +1Score > 38 indicates identity
Score > 31 indicates homology		 VAATTIK
1606 413.2127 1236.6163 1236.6238 -6.06 0 18 0.6 +1Score > 41 indicates identity
Score > 28 indicates homology		 SLFAQTVLAATN + 2 Deamidated (NQ)
1187 1035.5885 1034.5812 1034.5873 -5.84 1 18 0.27 +1Score > 38 indicates identity
Score > 25 indicates homology		 KQFLLSSGR
1345 375.8660 1124.5762 1124.5866 -9.25 0 18 0.16 +1Score > 40 indicates identity
Score > 23 indicates homology		 IAVNYQLFR + 2 Deamidated (NQ)
2765 599.3325 1794.9757 1794.9727 1.67 1 18 0.94 +1Score > 37 indicates identity
Score > 30 indicates homology		 AIIGQQVSLLANLAPRE + 3 Deamidated (NQ)
3741 799.4200 2395.2382 2395.2707 -13.6 1 18 0.78 +1Score > 38 indicates identity
Score > 30 indicates homology		 LQDLLQQNNLDSIAKQAVLDR + Deamidated (NQ)
532 381.2074 760.4002 760.3967 4.71 1 18 0.35 +1Score > 44 indicates identity
Score > 26 indicates homology		 TVNELGK + Deamidated (NQ)
388 346.7118 691.4090 691.4057 4.80 0 18 0.14 +1Score > 37 indicates identity
Score > 22 indicates homology		 VWIFK
1297 551.3021 1100.5896 1100.5965 -6.22 1 18 0.94 +1Score > 41 indicates identity
Score > 30 indicates homology		 LVLTDELVAE
1457 586.2855 1170.5564 1170.5418 12.5 0 18 0.64 +1Score > 40 indicates identity
Score > 29 indicates homology		 DGGPAIYGHQR + Deamidated (NQ)
1967 472.5831 1414.7275 1414.7416 -9.97 1 18 0.86 +1Score > 41 indicates identity
Score > 30 indicates homology		 ARASATVAPTNDLK + Deamidated (NQ)
1528 403.1927 1206.5563 1206.5703 -11.6 0 18 0.85 +1Score > 41 indicates identity
Score > 30 indicates homology		 DLDMSFNPLR
1769 660.3083 1318.6020 1318.6153 -10.1 0 18 0.6 +1Score > 40 indicates identity
Score > 28 indicates homology		 TLDISDADWQR
2353 794.4070 1586.7994 1586.8192 -12.4 1 18 0.86 +1Score > 41 indicates identity
Score > 30 indicates homology		 FTGVQQTAVPAPIEK + 2 Deamidated (NQ)
714 423.2295 844.4444 844.4515 -8.34 1 18 0.85 +1Score > 43 indicates identity
Score > 30 indicates homology		 LAGSADRR
1007 480.7739 959.5332 959.5287 4.71 1 18 0.87 +1Score > 41 indicates identity
Score > 30 indicates homology		 ILTGNKADK + Deamidated (NQ)
661 409.7181 817.4216 817.4181 4.31 0 18 0.82 +1Score > 44 indicates identity
Score > 30 indicates homology		 VISNADAK + Deamidated (NQ)
1652 421.2314 1260.6724 1260.6748 -1.89 1 18 0.71 +1Score > 40 indicates identity
Score > 29 indicates homology		 MVVSIAEDLLR + Oxidation (M)
1176 1031.5151 1030.5078 1030.5043 3.39 1 18 0.86 +1Score > 41 indicates identity
Score > 30 indicates homology		 TVQESPQSR
1358 566.3383 1130.6620 1130.6448 15.3 0 18 0.47 +1Score > 36 indicates identity
Score > 27 indicates homology		 LIFGIQAVDR
1037 487.3124 972.6102 972.5968 13.9 1 18 0.21 +1Score > 30 indicates identity
Score > 24 indicates homology		 ILKTGISNK
1458 1171.5648 1170.5575 1170.5418 13.5 0 18 0.93 +1Score > 40 indicates identity
Score > 30 indicates homology		 DGGPAIYGHQR + Deamidated (NQ)
1275 545.2817 1088.5488 1088.5648 -14.6 1 18 0.7 +1Score > 42 indicates identity
Score > 29 indicates homology		 NICANLVKR + 2 Deamidated (NQ)
1379 570.3272 1138.6398 1138.6458 -5.25 1 18 0.34 +1Score > 36 indicates identity
Score > 26 indicates homology		 IHAQLATRTK + Deamidated (NQ)
1766 440.2207 1317.6403 1317.6486 -6.30 1 18 0.69 +1Score > 41 indicates identity
Score > 29 indicates homology		 NLMEDILNDLK + Deamidated (NQ)
458 365.2205 728.4264 728.4221 5.96 0 18 0.27 +1Score > 42 indicates identity
Score > 25 indicates homology		 SPVWLK
1757 328.6910 1310.7349 1310.7558 -15.9 0 18 0.45 +1Score > 37 indicates identity
Score > 27 indicates homology		 RPNILASGIQIK + 2 Deamidated (NQ)
2396 806.9106 1611.8066 1611.8243 -11.0 1 18 0.57 +1Score > 40 indicates identity
Score > 28 indicates homology		 ISADEVQDPILQAVL + 2 Deamidated (NQ)
970 474.2365 946.4584 946.4720 -14.3 0 18 0.89 +1Score > 42 indicates identity
Score > 30 indicates homology		 VSTLQNQR + 2 Deamidated (NQ)
2192 508.2483 1521.7231 1521.7419 -12.3 0 18 0.95 +1Score > 41 indicates identity
Score > 30 indicates homology		 LAVMDITAASLCLE + Oxidation (M)
2018 480.9214 1439.7424 1439.7296 8.85 1 18 0.99 +1Score > 40 indicates identity
Score > 30 indicates homology		 ISDFPTIYSLRE
630 401.2497 800.4848 800.4909 -7.51 0 18 0.44 +1Score > 40 indicates identity
Score > 27 indicates homology		 VVLAFPR
819 444.7151 887.4156 887.4236 -8.94 1 18 0.53 +1Score > 39 indicates identity
Score > 28 indicates homology		 LNESQAPK + 2 Deamidated (NQ)
2328 790.4498 1578.8850 1578.8729 7.68 1 18 0.17 +1Score > 36 indicates identity
Score > 22 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
2632 574.9855 1721.9347 1721.9451 -6.07 0 18 0.53 +1Score > 37 indicates identity
Score > 27 indicates homology		 DAVTTIISDLLSVTFK
2375 534.2865 1599.8377 1599.8403 -1.62 1 18 0.51 +1Score > 40 indicates identity
Score > 27 indicates homology		 AIVSSMIQDLSKHR + Oxidation (M)
1494 593.3487 1184.6828 1184.6951 -10.4 0 18 0.52 +1Score > 38 indicates identity
Score > 27 indicates homology		 VVAGLDIMIVR
1718 647.3259 1292.6372 1292.6500 -9.84 1 18 0.13 +1Score > 42 indicates identity
Score > 21 indicates homology		 DAILVNVYQEK + 2 Deamidated (NQ)
3817 617.5766 2466.2773 2466.2312 18.7 0 18 0.31 +1Score > 37 indicates identity
Score > 25 indicates homology		 DPSIIVIDANQSMLDVHQSILR + 3 Deamidated (NQ)
322 330.7079 659.4012 659.3966 7.02 0 18 0.94 +1Score > 37 indicates identity
Score > 30 indicates homology		 ISVSVR
2191 507.9491 1520.8255 1520.8450 -12.8 1 18 0.25 +1Score > 38 indicates identity
Score > 24 indicates homology		 AISAFTLAVETALSK
1558 609.8077 1217.6008 1217.5962 3.85 0 18 0.68 +1Score > 42 indicates identity
Score > 28 indicates homology		 QGLANLMDDLK + Deamidated (NQ)
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