MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1201 348.5515 1042.6327 1042.6135 18.4 1 21 0.47 +1Score > 36 indicates identity
Score > 30 indicates homology		 LAKLQSQVR + Deamidated (NQ)
419 352.6953 703.3760 703.3752 1.21 1 21 0.73 +1Score > 43 indicates identity
Score > 32 indicates homology		 VEQISK + Deamidated (NQ)
1141 339.5154 1015.5244 1015.5298 -5.33 1 21 0.82 +1Score > 42 indicates identity
Score > 33 indicates homology		 AAAATLNEVR + Deamidated (NQ)
1378 570.3270 1138.6394 1138.6346 4.27 1 21 0.65 +1Score > 37 indicates identity
Score > 32 indicates homology		 IASLEAHLASK
2363 797.3994 1592.7842 1592.8046 -12.8 1 21 0.088 +1Score > 41 indicates identity
Score > 23 indicates homology		 AIANAITLEFTDGTR + Deamidated (NQ)
1380 570.3273 1138.6400 1138.6346 4.80 1 21 0.65 +1Score > 36 indicates identity
Score > 32 indicates homology		 IASLEAHLASK
1715 645.8604 1289.7062 1289.7013 3.84 1 21 0.88 +1Score > 39 indicates identity
Score > 33 indicates homology		 ALMGLIEATSLR + Oxidation (M)
2280 781.9553 1561.8960 1561.8715 15.7 1 21 0.53 +1Score > 34 indicates identity
Score > 31 indicates homology		 LFSIVALAASTNVEK
1978 474.5750 1420.7032 1420.7310 -19.6 1 21 0.56 +1Score > 41 indicates identity
Score > 31 indicates homology		 SSELNTIVQQFR
771 434.2594 866.5042 866.4974 7.93 1 21 0.77 +1Score > 35 indicates identity
Score > 32 indicates homology		 HEILSLR
1222 529.7927 1057.5708 1057.5655 5.04 1 21 0.36 +1Score > 42 indicates identity
Score > 29 indicates homology		 IESVGSLKPE
954 470.7844 939.5542 939.5502 4.36 0 21 0.14 +1Score > 36 indicates identity
Score > 25 indicates homology		 PTINQLVR
1088 500.2740 998.5334 998.5185 15.0 1 21 0.45 +1Score > 40 indicates identity
Score > 30 indicates homology		 WQIDRLAP + Deamidated (NQ)
282 319.1695 636.3244 636.3231 2.11 0 21 0.17 +1Score > 43 indicates identity
Score > 26 indicates homology		 YIADR
2266 778.4466 1554.8786 1554.8729 3.68 1 21 0.96 +1Score > 37 indicates identity
Score > 33 indicates homology		 RTGQIVLVNNIQAK + 2 Deamidated (NQ)
2305 525.2953 1572.8641 1572.8875 -14.9 0 21 0.42 +1Score > 38 indicates identity
Score > 30 indicates homology		 LPITTFGAGSNIIIR + Deamidated (NQ)
2506 555.9943 1664.9611 1664.9535 4.55 0 21 0.16 +1Score > 33 indicates identity
Score > 25 indicates homology		 SMPPAVLAGQIIDLLK
1233 532.2772 1062.5398 1062.5379 1.80 1 21 0.73 +1Score > 42 indicates identity
Score > 32 indicates homology		 AIMKGTGQLE + Oxidation (M)
815 444.2509 886.4872 886.4872 0.023 1 21 0.9 +1Score > 42 indicates identity
Score > 33 indicates homology		 LEQGSVVR
1277 545.2833 1088.5520 1088.5614 -8.62 0 21 0.73 +1Score > 42 indicates identity
Score > 32 indicates homology		 NNGSILAFPR + Deamidated (NQ)
1759 657.3462 1312.6778 1312.6623 11.9 1 21 0.82 +1Score > 40 indicates identity
Score > 32 indicates homology		 QGLARDGKPIQE + 2 Deamidated (NQ)
508 375.7249 749.4352 749.4323 3.90 1 21 0.45 +1Score > 36 indicates identity
Score > 30 indicates homology		 KFLTIE
409 351.7015 701.3884 701.3820 9.14 0 21 0.43 +1Score > 42 indicates identity
Score > 30 indicates homology		 VSNVQR
552 385.7623 769.5100 769.5061 5.09 0 21 0.89 +1Score > 35 indicates identity
Score > 33 indicates homology		 LAAAIIAK
2653 866.9454 1731.8762 1731.8890 -7.38 0 21 0.64 +1Score > 41 indicates identity
Score > 31 indicates homology		 LVQAVIAGGSSSLINSSE + Deamidated (NQ)
1145 508.7932 1015.5718 1015.5550 16.6 0 21 0.83 +1Score > 41 indicates identity
Score > 32 indicates homology		 ISNQLDVVK + Deamidated (NQ)
1658 631.8246 1261.6346 1261.6489 -11.3 1 21 0.18 +1Score > 42 indicates identity
Score > 26 indicates homology		 EPQTPLYKMR
2036 725.4097 1448.8048 1448.7974 5.16 1 21 0.45 +1Score > 37 indicates identity
Score > 30 indicates homology		 TTTTTIISKILQE + Deamidated (NQ)
1887 687.3571 1372.6996 1372.6987 0.72 1 21 0.98 +1Score > 41 indicates identity
Score > 33 indicates homology		 WITDINNQKIE
241 616.3433 615.3360 615.3452 -15.0 1 21 0.87 +1Score > 44 indicates identity
Score > 32 indicates homology		 KGNAAR
2020 721.3383 1440.6620 1440.6820 -13.8 1 21 0.52 +1Score > 40 indicates identity
Score > 30 indicates homology		 GAGMTPFSRFADGK
1631 624.8390 1247.6634 1247.6510 9.99 1 20 0.13 +1Score > 41 indicates identity
Score > 24 indicates homology		 IDKNDLDIFR
1549 304.6742 1214.6677 1214.6619 4.80 1 20 0.37 +1Score > 40 indicates identity
Score > 29 indicates homology		 ALIQSAIGSAER
252 311.1907 620.3668 620.3646 3.63 0 20 0.53 +1Score > 36 indicates identity
Score > 30 indicates homology		 VFSIR
1179 345.5027 1033.4863 1033.4862 0.034 1 20 0.94 +1Score > 41 indicates identity
Score > 33 indicates homology		 IDAMGGEIGR + Oxidation (M)
1969 708.4018 1414.7890 1414.7667 15.8 0 20 0.23 +1Score > 38 indicates identity
Score > 27 indicates homology		 LLQDIDTLLTNR + Deamidated (NQ)
787 437.7234 873.4322 873.4304 2.09 1 20 0.72 +1Score > 43 indicates identity
Score > 31 indicates homology		 QSENIGAR
857 451.7649 901.5152 901.5120 3.56 1 20 0.58 +1Score > 41 indicates identity
Score > 30 indicates homology		 VSVVKQIE + Deamidated (NQ)
2623 574.2999 1719.8779 1719.9018 -13.9 1 20 0.26 +1Score > 40 indicates identity
Score > 27 indicates homology		 LIGYMLTHLEFINR + Deamidated (NQ)
211 301.1880 600.3614 600.3707 -15.5 1 20 0.65 +1Score > 44 indicates identity
Score > 31 indicates homology		 IAGGKR
1919 695.8873 1389.7600 1389.7616 -1.10 1 20 0.57 +1Score > 39 indicates identity
Score > 30 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
2327 790.4493 1578.8840 1578.8729 7.04 1 20 0.39 +1Score > 36 indicates identity
Score > 29 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
1704 643.8739 1285.7332 1285.7242 7.06 0 20 0.77 +1Score > 37 indicates identity
Score > 32 indicates homology		 LLTTSGLITDPR
926 464.7277 927.4408 927.4549 -15.2 1 20 0.087 +1Score > 40 indicates identity
Score > 22 indicates homology		 TLSYETSK
1180 517.7512 1033.4878 1033.4750 12.4 1 20 0.21 +1Score > 41 indicates identity
Score > 26 indicates homology		 NVEMALNVE + Oxidation (M)
1389 571.8604 1141.7062 1141.6958 9.16 1 20 0.67 +1Score > 31 indicates identity SLIIVNEILK + Deamidated (NQ)
1120 506.3227 1010.6308 1010.6488 -17.8 0 20 0.9 +1Score > 33 indicates identity
Score > 32 indicates homology		 VILTSPLIR
1435 388.2092 1161.6058 1161.6281 -19.2 1 20 0.46 +1Score > 41 indicates identity
Score > 29 indicates homology		 FLQLAVDKVE + Deamidated (NQ)
997 479.2745 956.5344 956.5291 5.59 1 20 0.9 +1Score > 41 indicates identity
Score > 32 indicates homology		 LGDVVEAVR
1822 447.9218 1340.7436 1340.7664 -17.0 0 20 0.41 +1Score > 39 indicates identity
Score > 29 indicates homology		 VTVPDLSALAVTR
231 305.1721 608.3296 608.3282 2.38 0 20 0.96 +1Score > 38 indicates identity
Score > 32 indicates homology		 YGTIR
2350 793.4348 1584.8550 1584.8246 19.2 1 20 0.29 +1Score > 39 indicates identity
Score > 27 indicates homology		 DKGALDNLLAQLIAE + 2 Deamidated (NQ)
1391 571.8618 1141.7090 1141.6958 11.6 1 20 0.26 +1Score > 30 indicates identity
Score > 27 indicates homology		 SLIIVNEILK + Deamidated (NQ)
861 452.2500 902.4854 902.4934 -8.77 1 20 0.96 +1Score > 42 indicates identity
Score > 32 indicates homology		 TARDAITR
498 374.2439 746.4732 746.4691 5.62 0 20 0.39 +1Score > 32 indicates identity
Score > 28 indicates homology		 AGFVILK
2061 731.8695 1461.7244 1461.7245 -0.048 1 20 0.77 +1Score > 41 indicates identity
Score > 31 indicates homology		 LNLQKMSNNNLR + 2 Deamidated (NQ); Oxidation (M)
1020 322.5321 964.5745 964.5593 15.7 0 20 0.24 +1Score > 34 indicates identity
Score > 26 indicates homology		 SSLFIIASK
312 328.6912 655.3678 655.3653 3.85 0 20 0.45 +1Score > 37 indicates identity
Score > 29 indicates homology		 IGDPVR
1162 512.2612 1022.5078 1022.4920 15.5 1 20 0.95 +1Score > 41 indicates identity
Score > 32 indicates homology		 AISEVFNNK + 2 Deamidated (NQ)
1357 566.2866 1130.5586 1130.5567 1.68 1 20 0.78 +1Score > 41 indicates identity
Score > 31 indicates homology		 VVELNASDQR + Deamidated (NQ)
408 702.3939 701.3866 701.3820 6.59 1 20 0.89 +1Score > 42 indicates identity
Score > 32 indicates homology		 KADAAAR
1335 374.5499 1120.6279 1120.6393 -10.2 0 20 0.85 +1Score > 38 indicates identity
Score > 32 indicates homology		 FLLAQNFIR
457 365.2140 728.4134 728.4181 -6.35 0 20 0.61 +1Score > 42 indicates identity
Score > 30 indicates homology		 IISGSPR
1140 508.7685 1015.5224 1015.5298 -7.26 0 20 0.4 +1Score > 42 indicates identity
Score > 28 indicates homology		 LGLSADDVAR
1617 414.2380 1239.6922 1239.6935 -1.08 1 20 0.93 +1Score > 38 indicates identity
Score > 32 indicates homology		 QKAVVAGRPDAK + Deamidated (NQ)
1289 549.2988 1096.5830 1096.5625 18.7 1 20 0.21 +1Score > 39 indicates identity
Score > 26 indicates homology		 VVSHVRSQGE
1866 683.9116 1365.8086 1365.7868 16.0 0 20 0.06 +1Score > 32 indicates identity
Score > 20 indicates homology		 GLVSTPAGPVNLLK + Deamidated (NQ)
434 358.2061 714.3976 714.4024 -6.68 1 20 0.75 +1Score > 42 indicates identity
Score > 31 indicates homology		 AIGGEIR
2066 488.2890 1461.8452 1461.8555 -7.04 1 20 0.22 +1Score > 34 indicates identity
Score > 26 indicates homology		 GINLSPLHQKLLK + 2 Deamidated (NQ)
2161 504.6069 1510.7989 1510.7932 3.73 1 20 0.12 +1Score > 39 indicates identity
Score > 23 indicates homology		 VDFPFVQRYLAR + Deamidated (NQ)
1174 515.3153 1028.6160 1028.6019 13.8 1 20 0.65 +1Score > 37 indicates identity
Score > 30 indicates homology		 FVELPLVGR
845 899.5085 898.5012 898.5058 -5.13 0 20 0.88 +1Score > 39 indicates identity
Score > 32 indicates homology		 LSAMKPPR
2625 574.3008 1719.8806 1719.9018 -12.3 1 20 0.97 +1Score > 40 indicates identity
Score > 32 indicates homology		 LIGYMLTHLEFINR + Deamidated (NQ)
1590 615.8381 1229.6616 1229.6476 11.4 1 20 0.24 +1Score > 41 indicates identity
Score > 26 indicates homology		 AIGRTDLAGGSGR
2042 484.5854 1450.7344 1450.7589 -16.9 1 20 0.35 +1Score > 41 indicates identity
Score > 28 indicates homology		 LSTMLTTNAILKE + Deamidated (NQ); Oxidation (M)
1881 686.3680 1370.7214 1370.7154 4.43 0 20 0.66 +1Score > 40 indicates identity
Score > 30 indicates homology		 TPQSDASLVANLR
1455 585.8308 1169.6470 1169.6655 -15.8 1 20 0.27 +1Score > 40 indicates identity
Score > 26 indicates homology		 NIPALSQKLGK + 2 Deamidated (NQ)
1183 1035.4921 1034.4848 1034.4815 3.22 1 20 0.99 +1Score > 41 indicates identity
Score > 32 indicates homology		 CTADDLKGR
3008 652.7150 1955.1232 1955.1237 -0.28 1 20 0.36 +1Score > 31 indicates identity
Score > 28 indicates homology		 DMAGTLKPAAAKLLISSIR
1247 536.7958 1071.5770 1071.5924 -14.3 1 20 0.81 +1Score > 42 indicates identity
Score > 31 indicates homology		 IVNESLGALR + Deamidated (NQ)
3709 789.4012 2365.1818 2365.1584 9.89 1 20 0.19 +1Score > 38 indicates identity
Score > 25 indicates homology		 DGLPMTLPVRLGADADSANPGGPK + Deamidated (NQ); Oxidation (M)
1819 669.8264 1337.6382 1337.6649 -19.9 1 20 0.89 +1Score > 41 indicates identity
Score > 32 indicates homology		 MEFQTTLPSIR + Oxidation (M)
625 401.2468 800.4790 800.4868 -9.69 1 20 0.69 +1Score > 42 indicates identity
Score > 31 indicates homology		 ISNALKR
2179 506.2686 1515.7840 1515.7868 -1.85 1 20 0.48 +1Score > 41 indicates identity
Score > 29 indicates homology		 WGTPITMDAAVKAR
1661 632.8149 1263.6152 1263.6221 -5.42 1 20 0.85 +1Score > 41 indicates identity
Score > 31 indicates homology		 FGHRGSNHPVR + Deamidated (NQ)
590 394.2611 786.5076 786.5004 9.28 0 20 0.91 +1Score > 33 indicates identity
Score > 32 indicates homology		 APFVLIK
529 380.2544 758.4942 758.4902 5.37 0 19 0.36 +1Score > 30 indicates identity
Score > 28 indicates homology		 SVLSILK
1070 497.2983 992.5820 992.5767 5.40 0 19 0.92 +1Score > 35 indicates identity
Score > 32 indicates homology		 LHLNQLVR + Deamidated (NQ)
3626 1156.5933 2311.1720 2311.1842 -5.25 1 19 0.1 +1Score > 38 indicates identity
Score > 22 indicates homology		 LLEPNAMVLSTVNEVNQPSTR
209 300.7086 599.4026 599.4006 3.36 0 19 0.95 +1Score > 40 indicates identity
Score > 32 indicates homology		 ALGVLK
319 330.1904 658.3662 658.3762 -15.1 1 19 0.65 +1Score > 45 indicates identity
Score > 30 indicates homology		 AVKQGR + Deamidated (NQ)
1320 558.3375 1114.6604 1114.6598 0.62 1 19 0.12 +1Score > 36 indicates identity
Score > 23 indicates homology		 ALDVLTDLKK
1526 602.8558 1203.6970 1203.6863 8.92 1 19 0.36 +1Score > 35 indicates identity
Score > 27 indicates homology		 LSIQIFKGGIE
736 854.4769 853.4696 853.4657 4.56 0 19 0.27 +1Score > 37 indicates identity
Score > 26 indicates homology		 APAAAPAASK
2262 518.6089 1552.8049 1552.7742 19.8 1 19 0.12 +1Score > 40 indicates identity
Score > 23 indicates homology		 MVSGMVKHITGSYK + Oxidation (M)
930 465.2791 928.5436 928.5342 10.2 1 19 0.98 +1Score > 40 indicates identity
Score > 32 indicates homology		 GAVLTEALR
1805 444.5923 1330.7551 1330.7344 15.5 1 19 0.18 +1Score > 36 indicates identity
Score > 24 indicates homology		 TTVQIKGDLLDK + Deamidated (NQ)
264 315.2035 628.3924 628.3908 2.63 0 19 0.87 +1Score > 42 indicates identity
Score > 31 indicates homology		 AAGLIGK
1003 320.1921 957.5545 957.5607 -6.52 1 19 0.71 +1Score > 41 indicates identity
Score > 30 indicates homology		 KIVSIDAGR
1258 541.7670 1081.5194 1081.5404 -19.3 1 19 0.87 +1Score > 40 indicates identity
Score > 31 indicates homology		 KVDLDSYSR
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