MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2322 789.4289 1576.8432 1576.8395 2.37 1 23 0.14 +1Score > 39 indicates identity
Score > 27 indicates homology		 FLGNLMQLLNNKR + Deamidated (NQ); Oxidation (M)
684 416.7523 831.4900 831.4888 1.50 0 23 0.61 +1Score > 38 indicates identity
Score > 34 indicates homology		 TLGLMLGK
2564 564.6458 1690.9156 1690.8890 15.7 1 23 0.11 +1Score > 37 indicates identity
Score > 26 indicates homology		 IDGVVYKVNSLAQQR + 2 Deamidated (NQ)
1186 518.2793 1034.5440 1034.5284 15.1 1 23 0.85 +1Score > 41 indicates identity
Score > 35 indicates homology		 AIIQGKQFE + 2 Deamidated (NQ)
1013 481.7349 961.4552 961.4505 4.94 0 23 0.32 +1Score > 41 indicates identity
Score > 31 indicates homology		 NWSNDVVK + Deamidated (NQ)
190 569.3065 568.2992 568.2969 4.08 0 23 0.26 +1Score > 38 indicates identity
Score > 30 indicates homology		 DVHAK
1495 594.3203 1186.6260 1186.6346 -7.19 1 23 0.8 +1Score > 41 indicates identity
Score > 35 indicates homology		 AKASLIAQAWE
1723 432.2300 1293.6682 1293.6677 0.36 0 23 0.16 +1Score > 41 indicates identity
Score > 28 indicates homology		 ADHQGGLDGILAK
1143 508.7752 1015.5358 1015.5550 -18.8 0 23 0.22 +1Score > 42 indicates identity
Score > 29 indicates homology		 AGDVVQIVSK + Deamidated (NQ)
1540 606.3575 1210.7004 1210.6809 16.2 1 23 0.25 +1Score > 36 indicates identity
Score > 29 indicates homology		 VIVPEAITIQE
984 317.8357 950.4853 950.4821 3.30 1 23 0.28 +1Score > 41 indicates identity
Score > 30 indicates homology		 VNKNSGFGK + Deamidated (NQ)
655 407.7574 813.5002 813.4960 5.22 1 23 0.61 +1Score > 39 indicates identity
Score > 33 indicates homology		 LGAEVVVK
1469 587.3119 1172.6092 1172.6302 -17.9 0 23 0.18 +1Score > 42 indicates identity
Score > 28 indicates homology		 AALVSYLSGHR
1537 606.3483 1210.6820 1210.6822 -0.13 1 23 0.48 +1Score > 37 indicates identity
Score > 32 indicates homology		 QSILLRYYR
1021 483.7499 965.4852 965.4818 3.58 1 23 0.47 +1Score > 42 indicates identity
Score > 32 indicates homology		 KFQATDQK + Deamidated (NQ)
1734 434.2466 1299.7180 1299.7034 11.2 1 23 0.26 +1Score > 40 indicates identity
Score > 30 indicates homology		 QVGGLDEIILSR + Deamidated (NQ)
1875 685.3346 1368.6546 1368.6456 6.61 0 23 0.23 +1Score > 41 indicates identity
Score > 29 indicates homology		 HADQVLVMDQGR + Deamidated (NQ)
770 434.2590 866.5034 866.4974 7.01 1 23 0.8 +1Score > 36 indicates identity
Score > 34 indicates homology		 HEILISR
197 584.3428 583.3355 583.3330 4.39 0 23 0.097 +1Score > 32 indicates identity
Score > 25 indicates homology		 QLGVAP
1046 491.7418 981.4690 981.4556 13.7 1 23 0.1 +1Score > 41 indicates identity
Score > 26 indicates homology		 WEQYVTR + Deamidated (NQ)
1212 526.2579 1050.5012 1050.4982 2.95 1 23 0.56 +1Score > 41 indicates identity
Score > 33 indicates homology		 AGIYNEQVR + 2 Deamidated (NQ)
1329 560.7951 1119.5756 1119.5958 -18.0 1 23 0.33 +1Score > 42 indicates identity
Score > 31 indicates homology		 KIMQITNQK + Deamidated (NQ); Oxidation (M)
443 360.7071 719.3996 719.3966 4.21 1 23 0.25 +1Score > 41 indicates identity
Score > 29 indicates homology		 FGEVIR
668 412.7369 823.4592 823.4552 4.92 0 23 0.4 +1Score > 38 indicates identity
Score > 31 indicates homology		 HGITPTAK
1976 710.8431 1419.6716 1419.6729 -0.88 1 23 0.61 +1Score > 41 indicates identity
Score > 33 indicates homology		 ASTKDQLPTTNDK + 2 Deamidated (NQ)
1330 560.8054 1119.5962 1119.5924 3.44 1 23 0.49 +1Score > 41 indicates identity
Score > 32 indicates homology		 VAQLYLEQR + Deamidated (NQ)
2031 722.8694 1443.7242 1443.7358 -7.99 1 23 0.38 +1Score > 41 indicates identity
Score > 31 indicates homology		 WDVNIGELAGITR + Deamidated (NQ)
1244 536.7874 1071.5602 1071.5673 -6.54 0 23 0.48 +1Score > 42 indicates identity
Score > 32 indicates homology		 QTVQQAAQAK
720 846.4490 845.4417 845.4429 -1.39 0 23 0.97 +1Score > 42 indicates identity
Score > 35 indicates homology		 LGGQIMAR + Deamidated (NQ)
675 415.2444 828.4742 828.4593 18.1 0 23 0.46 +1Score > 42 indicates identity
Score > 32 indicates homology		 VVDAALIE
1108 504.7546 1007.4946 1007.4924 2.28 1 23 0.38 +1Score > 42 indicates identity
Score > 31 indicates homology		 DAFQAELSK
2271 520.6166 1558.8280 1558.8202 4.96 1 23 0.74 +1Score > 39 indicates identity
Score > 34 indicates homology		 SNVGTLGQILGETIR + 2 Deamidated (NQ)
934 465.7868 929.5590 929.5545 4.86 1 23 0.49 +1Score > 38 indicates identity
Score > 32 indicates homology		 KLSAALQAK + Deamidated (NQ)
1397 574.3145 1146.6144 1146.6365 -19.2 1 23 0.52 +1Score > 41 indicates identity
Score > 32 indicates homology		 SLLMRKPMR + Oxidation (M)
394 697.3371 696.3298 696.3265 4.83 0 23 0.73 +1Score > 37 indicates identity
Score > 34 indicates homology		 MANAYK
1299 551.8193 1101.6240 1101.6182 5.29 1 23 0.2 +1Score > 40 indicates identity
Score > 28 indicates homology		 AKFTSNLPPK
1439 583.2762 1164.5378 1164.5550 -14.7 1 23 0.21 +1Score > 41 indicates identity
Score > 28 indicates homology		 LYQALLEGQE + 2 Deamidated (NQ)
1440 389.2168 1164.6286 1164.6503 -18.6 1 23 0.96 +1Score > 39 indicates identity
Score > 35 indicates homology		 HADQVLLLEK
2385 805.3812 1608.7478 1608.7600 -7.53 1 22 0.15 +1Score > 40 indicates identity
Score > 27 indicates homology		 KMGMNIINDDITGR + 2 Oxidation (M)
1251 359.5337 1075.5793 1075.5761 2.97 0 22 0.66 +1Score > 41 indicates identity
Score > 33 indicates homology		 NISSSIDIVK + Deamidated (NQ)
2195 761.8715 1521.7284 1521.7419 -8.81 0 22 0.12 +1Score > 41 indicates identity
Score > 26 indicates homology		 LAVMDITAASLCLE + Oxidation (M)
1500 398.2024 1191.5854 1191.5666 15.7 1 22 0.98 +1Score > 41 indicates identity
Score > 35 indicates homology		 VQDRMSNGLR + Deamidated (NQ); Oxidation (M)
2065 731.9294 1461.8442 1461.8303 9.53 1 22 0.77 +1Score > 34 indicates identity SYRQQINSILLK
1646 629.8571 1257.6996 1257.6816 14.4 0 22 0.5 +1Score > 39 indicates identity
Score > 32 indicates homology		 LVIAVANDSNIK + 2 Deamidated (NQ)
1726 648.8696 1295.7246 1295.7085 12.5 1 22 0.52 +1Score > 37 indicates identity
Score > 32 indicates homology		 QPVLQVDEQLK
1873 685.3315 1368.6484 1368.6456 2.08 0 22 0.29 +1Score > 40 indicates identity
Score > 29 indicates homology		 HADQVLVMDQGR + Deamidated (NQ)
2274 781.4211 1560.8276 1560.8010 17.1 0 22 0.12 +1Score > 40 indicates identity
Score > 26 indicates homology		 IFIQQLNLWCPK + 2 Deamidated (NQ)
691 419.1997 836.3848 836.3698 18.0 0 22 0.079 +1Score > 41 indicates identity
Score > 24 indicates homology		 NAMDTAAK + Oxidation (M)
206 595.3115 594.3042 594.3047 -0.81 0 22 0.4 +1Score > 40 indicates identity
Score > 31 indicates homology		 TVMTK + Oxidation (M)
522 379.7272 757.4398 757.4446 -6.27 1 22 0.46 +1Score > 42 indicates identity
Score > 31 indicates homology		 AARIQAK + Deamidated (NQ)
183 559.3230 558.3157 558.3125 5.72 0 22 0.5 +1Score > 45 indicates identity
Score > 32 indicates homology		 AGAAAAK
428 357.2204 712.4262 712.4232 4.34 0 22 0.12 +1Score > 34 indicates identity
Score > 25 indicates homology		 SPIIQR
937 466.7660 931.5174 931.5161 1.48 0 22 0.3 +1Score > 40 indicates identity
Score > 29 indicates homology		 MQLIATVR + Deamidated (NQ)
371 344.1938 686.3730 686.3711 2.80 0 22 0.67 +1Score > 44 indicates identity
Score > 33 indicates homology		 VAASSPR
1535 606.3438 1210.6730 1210.6822 -7.56 1 22 0.67 +1Score > 38 indicates identity
Score > 33 indicates homology		 QSILLRYYR
1131 508.2882 1014.5618 1014.5611 0.79 1 22 0.25 +1Score > 41 indicates identity
Score > 29 indicates homology		 AAGVKWDLR
284 637.3320 636.3247 636.3231 2.52 0 22 0.76 +1Score > 41 indicates identity
Score > 33 indicates homology		 YPTTR
347 673.4021 672.3948 672.3918 4.43 1 22 0.91 +1Score > 41 indicates identity
Score > 34 indicates homology		 KQIQR + Deamidated (NQ)
594 788.4244 787.4171 787.4188 -2.16 0 22 0.96 +1Score > 43 indicates identity
Score > 34 indicates homology		 QGVLTDR
1698 642.8288 1283.6430 1283.6357 5.73 1 22 0.35 +1Score > 40 indicates identity
Score > 30 indicates homology		 NIPADLENLQR + 2 Deamidated (NQ)
961 472.2477 942.4808 942.4770 4.04 1 22 0.24 +1Score > 39 indicates identity
Score > 28 indicates homology		 NLLEDPSR
972 316.5041 946.4905 946.4872 3.43 1 22 0.16 +1Score > 42 indicates identity
Score > 27 indicates homology		 FGIDPDKR
293 322.2109 642.4072 642.4177 -16.2 1 22 0.21 +1Score > 42 indicates identity
Score > 28 indicates homology		 KGAVIR
2113 744.9059 1487.7972 1487.7694 18.7 1 22 0.41 +1Score > 40 indicates identity
Score > 30 indicates homology		 MSAVKVQLVPWTE + Deamidated (NQ)
266 630.3959 629.3886 629.3860 4.09 0 22 0.91 +1Score > 42 indicates identity
Score > 34 indicates homology		 AGILTR
1054 495.2820 988.5494 988.5342 15.4 0 22 0.36 +1Score > 40 indicates identity
Score > 30 indicates homology		 LYGDPLGVR
982 475.7532 949.4918 949.4869 5.25 1 22 0.15 +1Score > 42 indicates identity
Score > 26 indicates homology		 AYLDEAIR
3184 687.7038 2060.0896 2060.1001 -5.12 0 22 0.54 +1Score > 38 indicates identity
Score > 32 indicates homology		 QISLLGSTGSIGTQTLDVIR + 2 Deamidated (NQ)
298 322.7025 643.3904 643.4017 -17.5 0 22 0.54 +1Score > 43 indicates identity
Score > 32 indicates homology		 IAAITR
295 322.2119 642.4092 642.4064 4.38 0 22 0.77 +1Score > 41 indicates identity
Score > 33 indicates homology		 AIAGLAK
922 464.2638 926.5130 926.5073 6.24 1 22 0.22 +1Score > 39 indicates identity
Score > 28 indicates homology		 TKLQNPPK + 2 Deamidated (NQ)
379 345.2034 688.3922 688.3908 2.09 0 22 0.74 +1Score > 41 indicates identity
Score > 33 indicates homology		 VAFPAGK
2924 631.9724 1892.8954 1892.8839 6.05 0 22 0.15 +1Score > 40 indicates identity
Score > 26 indicates homology		 DCAGFRPAADIDPVYAR
794 877.5164 876.5091 876.4925 19.0 1 22 0.56 +1Score > 39 indicates identity
Score > 32 indicates homology		 KMGMLVAK
1880 686.3412 1370.6678 1370.6718 -2.86 1 22 0.83 +1Score > 40 indicates identity
Score > 33 indicates homology		 LNLNSFFESTAK + Deamidated (NQ)
1241 536.3129 1070.6112 1070.5972 13.2 1 22 0.78 +1Score > 39 indicates identity
Score > 33 indicates homology		 VLADLAVNEK
843 450.2371 898.4596 898.4436 17.9 1 22 0.51 +1Score > 39 indicates identity
Score > 31 indicates homology		 AYKFLQE + Deamidated (NQ)
316 329.7113 657.4080 657.4061 2.94 0 22 0.8 +1Score > 41 indicates identity
Score > 33 indicates homology		 ADIVIK
242 308.6829 615.3512 615.3592 -12.9 1 22 0.5 +1Score > 45 indicates identity
Score > 31 indicates homology		 VVAAEK
634 402.7549 803.4952 803.4905 5.88 0 22 0.21 +1Score > 34 indicates identity
Score > 27 indicates homology		 QFVLLGK
1125 507.3117 1012.6088 1012.6029 5.84 0 22 0.24 +1Score > 38 indicates identity
Score > 28 indicates homology		 IVVVSGGNLR
588 394.2607 786.5068 786.4963 13.4 1 22 0.49 +1Score > 33 indicates identity
Score > 31 indicates homology		 KGTALGLK
1152 510.7570 1019.4994 1019.5036 -4.07 0 22 0.61 +1Score > 42 indicates identity
Score > 32 indicates homology		 NGLTEPFSR
785 437.2460 872.4774 872.4603 19.6 1 22 0.93 +1Score > 43 indicates identity
Score > 34 indicates homology		 INAIEQGK + Deamidated (NQ)
2367 798.4595 1594.9044 1594.9229 -11.5 1 22 0.16 +1Score > 35 indicates identity
Score > 26 indicates homology		 LGMAGLRLGLLAGKPE
1514 601.3385 1200.6624 1200.6437 15.6 0 22 0.51 +1Score > 40 indicates identity
Score > 31 indicates homology		 MLNVVWNAVR
376 688.4274 687.4201 687.4167 5.00 0 21 0.84 +1Score > 40 indicates identity
Score > 33 indicates homology		 DVTLLK
1255 540.3027 1078.5908 1078.6022 -10.6 1 21 0.72 +1Score > 38 indicates identity
Score > 33 indicates homology		 IISQQFKSK + Deamidated (NQ)
565 388.2336 774.4526 774.4487 5.10 0 21 0.81 +1Score > 39 indicates identity
Score > 33 indicates homology		 ISDSILK
2746 889.4591 1776.9036 1776.8716 18.0 0 21 0.071 +1Score > 40 indicates identity
Score > 22 indicates homology		 MVASNNITGATVFVPQE
1728 432.9158 1295.7256 1295.7197 4.51 1 21 0.82 +1Score > 37 indicates identity
Score > 33 indicates homology		 QGKAGGAEPLLGAK
1486 592.3491 1182.6836 1182.6721 9.80 1 21 0.1 +1Score > 38 indicates identity
Score > 24 indicates homology		 QLVGQVAAEIR
1784 661.8290 1321.6434 1321.6370 4.90 1 21 0.22 +1Score > 41 indicates identity
Score > 27 indicates homology		 MNIGNMKATDVK + Deamidated (NQ)
524 380.2061 758.3976 758.3923 7.11 1 21 0.97 +1Score > 42 indicates identity
Score > 34 indicates homology		 QAGEVQK
3174 1030.5293 2059.0440 2059.0375 3.20 1 21 0.085 +1Score > 39 indicates identity
Score > 23 indicates homology		 GDKVQGDGDWKPIVQFLR + 2 Deamidated (NQ)
2344 528.9408 1583.8006 1583.7791 13.6 1 21 0.29 +1Score > 40 indicates identity
Score > 28 indicates homology		 RLQPTLNQALDANE + 2 Deamidated (NQ)
1927 466.5792 1396.7158 1396.6908 17.9 1 21 0.34 +1Score > 40 indicates identity
Score > 29 indicates homology		 KTLADLIYQMGE + Oxidation (M)
2269 779.9182 1557.8218 1557.8402 -11.8 1 21 0.17 +1Score > 40 indicates identity
Score > 26 indicates homology		 QVAALLKVTGDWQK + 2 Deamidated (NQ)
1538 404.5730 1210.6972 1210.7186 -17.7 1 21 0.88 +1Score > 36 indicates identity
Score > 33 indicates homology		 KWVGRPLIDK
1473 588.8194 1175.6242 1175.6411 -14.3 1 21 0.34 +1Score > 41 indicates identity
Score > 29 indicates homology		 AFAQVTVAGRR + Deamidated (NQ)
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