MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4817 1082.1389 4324.5265
4818 867.6634 4333.2806
4819 1084.3289 4333.2865
4820 1084.3289 4333.2865
4821 1085.5707 4338.2537
4822 868.8599 4339.2631
4823 869.4627 4342.2771
4824 869.4644 4342.2856
4825 870.2288 4346.1076
4826 1088.3239 4349.2665
4827 725.9047 4349.3845
4828 1089.5791 4354.2873
4829 1093.0905 4368.3329
4830 1093.5619 4370.2185
4831 875.4498 4372.2126
4832 876.4631 4377.2791
4833 1097.3134 4385.2245
4835 879.0605 4390.2661
4836 1098.5782 4390.2837
4837 1098.5802 4390.2917
4838 1098.5809 4390.2945
4839 1098.5815 4390.2969
4840 879.0668 4390.2976
4841 1098.5825 4390.3009
4842 1098.5833 4390.3041
4843 1098.5834 4390.3045
4844 879.0688 4390.3076
4845 879.0695 4390.3111
4846 1099.5482 4394.1637
4847 1102.5785 4406.2849
4848 1104.5620 4414.2189
4849 885.4349 4422.1381
4850 1107.5862 4426.3157
4851 886.4687 4427.3071
4852 739.0458 4428.2311
4853 1108.0892 4428.3277
4854 886.8545 4429.2361
4855 887.4487 4432.2071
4857 887.6174 4433.0506
4858 1109.5585 4434.2049
4859 1112.5884 4446.3245
4860 1112.5891 4446.3273
4861 1112.5935 4446.3449
4863 890.4620 4447.2736
4864 1112.8350 4447.3109
4865 890.4698 4447.3126
4866 890.4727 4447.3271
4867 1112.8391 4447.3273
4869 890.4736 4447.3316
4871 890.4760 4447.3436
4872 890.4794 4447.3606
4873 1113.0815 4448.2969
4874 1113.0833 4448.3041
4875 890.6699 4448.3131
4876 1113.3483 4449.3641
4878 1117.5870 4466.3189
4879 1121.6006 4482.3733
4885 901.8733 4504.3301
4886 901.8735 4504.3311
4887 902.4912 4507.4196
4888 904.4881 4517.4041
4889 753.9113 4517.4241
4890 904.6765 4518.3461
4891 1132.5004 4525.9725
4892 1138.6040 4550.3869
4895 1149.6013 4594.3761
4896 920.4630 4597.2786
4897 920.4777 4597.3521
4898 924.4696 4617.3116
4899 924.4758 4617.3426
4902 1160.5930 4638.3429
4903 928.6760 4638.3436
4904 929.4837 4642.3821
4905 930.5189 4647.5581
4906 930.6580 4648.2536
4907 930.7133 4648.5301
4908 1163.3881 4649.5233
4909 1165.0631 4656.2233
4912 940.4789 4697.3581
4913 1176.1060 4700.3949
4914 1177.6455 4706.5529
4915 942.4561 4707.2441
4916 1185.8799 4739.4905
4917 1190.1108 4756.4141
4919 958.8843 4789.3851
4920 959.1328 4790.6276
4924 967.4652 4832.2896
4925 808.7892 4846.6915
4926 808.9521 4847.6689
4927 809.2975 4849.7413
4928 979.9197 4894.5621
4929 981.7126 4903.5266
4930 981.7165 4903.5461
4932 818.4339 4904.5597
4933 818.4371 4904.5789
4934 820.9280 4919.5243
4935 985.9478 4924.7026
4936 707.3912 4944.6875
4937 825.1269 4944.7177
4938 990.5237 4947.5821
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