MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4665 902.4985 3605.9649
4666 722.2003 3605.9651
4667 722.2007 3605.9671
4668 902.5026 3605.9813
4669 903.4755 3609.8729
4670 903.4853 3609.9121
4671 905.9495 3619.7689
4672 906.4987 3621.9657
4678 910.5257 3638.0737
4681 914.7234 3654.8645
4682 914.9742 3655.8677
4683 914.9744 3655.8685
4686 916.5026 3661.9813
4687 733.4045 3661.9861
4689 916.9938 3663.9461
4690 917.4568 3665.7981
4691 919.0139 3672.0265
4692 921.0052 3679.9917
4694 737.3791 3681.8591
4695 737.3876 3681.9016
4696 738.3415 3686.6711
4698 931.8108 3723.2141
4699 932.0137 3724.0257
4701 932.5025 3725.9809
4703 936.9989 3743.9665
4704 937.5184 3746.0445
4705 938.7697 3751.0497
4706 940.5153 3758.0321
4707 942.4913 3765.9361
4708 943.5045 3769.9889
4711 947.5552 3786.1917
4712 947.7747 3787.0697
4715 952.4716 3805.8573
4717 952.7798 3807.0901
4718 953.4408 3809.7341
4719 957.5159 3826.0345
4720 957.7615 3827.0169
4721 958.5206 3830.0533
4722 768.6245 3838.0861
4723 961.0276 3840.0813
4726 967.5371 3866.1193
4728 777.2009 3880.9681
4729 973.5034 3889.9845
4730 973.5051 3889.9913
4731 650.3575 3896.1013
4732 780.4299 3897.1131
4733 975.4842 3897.9077
4734 975.5306 3898.0933
4735 976.0134 3900.0245
4737 781.2087 3901.0071
4739 977.7873 3907.1201
4740 980.0342 3916.1077
4742 981.5250 3922.0709
4744 989.7831 3955.1033
4746 990.7968 3959.1581
4747 991.0410 3960.1349
4750 1000.5179 3998.0425
4752 1002.0404 4004.1325
4753 1002.2571 4004.9993
4754 1002.2599 4005.0105
4755 1002.2802 4005.0917
4756 802.0309 4005.1181
4757 1002.3084 4005.2045
4758 1003.5305 4010.0929
4760 1003.9926 4011.9413
4762 804.4424 4017.1756
4763 805.2244 4021.0856
4765 1007.8102 4027.2117
4770 1015.5555 4058.1929
4771 1016.2642 4061.0277
4772 1016.5025 4061.9809
4774 1016.5402 4062.1317
4775 1016.5447 4062.1497
4776 815.6153 4073.0401
4777 1020.5328 4078.1021
4778 818.0462 4085.1946
4779 1022.3143 4085.2281
4780 1022.5588 4086.2061
4782 585.7269 4093.0374
4783 683.1819 4093.0477
4787 1031.3187 4121.2457
4788 1032.7825 4127.1009
4790 826.8293 4129.1101
4792 691.1833 4141.0561
4793 829.2200 4141.0636
4794 1041.0516 4160.1773
4797 1045.5679 4178.2425
4798 1046.5317 4182.0977
4799 1046.8406 4183.3333
4800 1047.2725 4185.0609
4802 1050.5206 4198.0533
4803 840.6210 4198.0686
4805 700.8532 4199.0755
4807 848.4410 4237.1686
4808 1060.3002 4237.1717
4809 848.4424 4237.1756
4811 848.4476 4237.2016
4812 1064.5603 4254.2121
4813 1074.0437 4292.1457
4814 862.0269 4305.0981
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