MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4494 1061.5238 3181.5496
4496 1061.5244 3181.5514
4498 796.3969 3181.5585
4499 796.4320 3181.6989
4500 1061.5743 3181.7011
4504 1068.2662 3201.7768
4506 801.4561 3201.7953
4508 801.9290 3203.6869
4509 802.4207 3205.6537
4510 1070.5587 3208.6543
4513 803.6666 3210.6373
4514 1071.5564 3211.6474
4516 1074.5651 3220.6735
4518 806.6876 3222.7213
4519 1075.5321 3223.5745
4523 1078.1824 3231.5254
4524 808.9454 3231.7525
4526 1078.5283 3232.5631
4527 809.4202 3233.6517
4528 1079.2537 3234.7393
4529 1079.5723 3235.6951
4532 1080.6027 3238.7863
4533 1081.5358 3241.5856
4534 813.4563 3249.7961
4536 813.4584 3249.8045
4537 813.4587 3249.8057
4538 815.4377 3257.7217
4539 652.5520 3257.7236
4541 817.4544 3265.7885
4542 1091.6078 3271.8016
4544 820.4232 3277.6637
4546 820.9280 3279.6829
4547 657.1485 3280.7061
4548 657.5588 3282.7576
4549 821.9496 3283.7693
4550 1097.6155 3289.8247
4551 824.4541 3293.7873
4553 826.4202 3301.6517
4554 551.8077 3304.8025
4556 1103.2753 3306.8041
4557 827.7084 3306.8045
4558 827.7086 3306.8053
4561 1103.6122 3307.8148
4563 829.2028 3312.7821
4568 830.4446 3317.7493
4573 834.4474 3333.7605
4574 834.4562 3333.7957
4576 836.2197 3340.8497
4577 1114.6239 3340.8499
4581 1117.2673 3348.7801
4582 838.2239 3348.8665
4583 838.2263 3348.8761
4584 838.9846 3351.9093
4585 1118.3143 3351.9211
4586 840.4729 3357.8625
4587 840.7139 3358.8265
4588 1120.6191 3358.8355
4589 1120.9429 3359.8069
4590 1121.6216 3361.8430
4595 1127.6044 3379.7914
4596 678.1528 3385.7276
4597 847.4410 3385.7349
4599 1131.2576 3390.7510
4603 683.5920 3412.9236
4608 858.4698 3429.8501
4613 861.2027 3440.7817
4614 861.4493 3441.7681
4615 861.4528 3441.7821
4616 861.4580 3441.8029
4617 1148.6359 3442.8859
4619 862.2205 3444.8529
4620 862.2233 3444.8641
4621 1149.5768 3445.7086
4622 690.1765 3445.8461
4624 867.4646 3465.8293
4625 1157.3081 3468.9025
4627 872.2546 3484.9893
4628 872.4429 3485.7425
4630 873.2263 3488.8761
4631 1164.6367 3490.8883
4632 875.4866 3497.9173
4635 878.0026 3507.9813
4637 1173.6511 3517.9315
4638 882.4915 3525.9369
4640 884.7300 3534.8909
4642 1182.2462 3543.7168
4644 886.9482 3543.7637
4645 887.2283 3544.8841
4646 887.2325 3544.9009
4648 889.2791 3553.0873
4651 893.4894 3569.9285
4652 715.1987 3570.9571
4653 893.7499 3570.9705
4657 894.4937 3573.9457
4658 894.5040 3573.9869
4659 718.6016 3587.9716
4660 901.5082 3602.0037
4662 902.2413 3604.9361
4663 722.1958 3605.9426
4664 722.1989 3605.9581
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