MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4213 941.4705 2821.3897
4215 943.5004 2827.4794
4217 944.5137 2830.5193
4218 946.1550 2835.4432
4220 946.4887 2836.4443
4223 946.4993 2836.4761
4227 946.5018 2836.4836
4228 946.5087 2836.5043
4229 710.3765 2837.4769
4232 710.3784 2837.4845
4239 948.8672 2843.5798
4240 948.8768 2843.6086
4241 948.8840 2843.6302
4243 950.8459 2849.5159
4248 953.8038 2858.3896
4254 955.8657 2864.5753
4255 955.8831 2864.6275
4256 956.1673 2865.4801
4260 958.4924 2872.4554
4262 719.1607 2872.6137
4263 958.5460 2872.6162
4264 958.8708 2873.5906
4267 959.5084 2875.5034
4270 721.4045 2881.5889
4282 723.9235 2891.6649
4294 580.9010 2899.4686
4297 726.1575 2900.6009
4298 968.1857 2901.5353
4299 726.4087 2901.6057
4300 968.5158 2902.5256
4304 728.1307 2908.4937
4305 728.1317 2908.4977
4306 970.5071 2908.4995
4307 970.5071 2908.4995
4309 728.4069 2909.5985
4310 971.4373 2911.2901
4312 972.8105 2915.4097
4316 974.4818 2920.4236
4317 974.5169 2920.5289
4320 975.5309 2923.5709
4321 976.1884 2925.5434
4324 976.8326 2927.4760
4339 984.5159 2950.5259
4347 742.3834 2965.5045
4351 991.2386 2970.6940
4352 991.8511 2972.5315
4357 992.8692 2975.5858
4359 745.1429 2976.5425
4364 996.5579 2986.6519
4367 747.9186 2987.6453
4368 997.5282 2989.5628
4371 998.5724 2992.6954
4374 1002.8600 3005.5582
4375 1003.2233 3006.6481
4380 1005.8925 3014.6557
4381 1006.2347 3015.6823
4382 1006.5720 3016.6942
4385 756.0955 3020.3529
4387 756.4201 3021.6513
4388 1008.2449 3021.7129
4391 1008.8535 3023.5387
4396 1010.8572 3029.5498
4397 607.3135 3031.5311
4400 760.6560 3038.5949
4404 1016.5292 3046.5658
4408 763.4337 3049.7057
4411 1018.8339 3053.4799
4413 1021.5261 3061.5565
4414 1021.5261 3061.5565
4420 1024.2318 3069.6736
4421 768.4303 3069.6921
4422 769.8719 3075.4585
4426 770.8905 3079.5329
4430 1028.2550 3081.7432
4431 1028.5480 3082.6222
4432 771.6945 3082.7489
4433 1029.5071 3085.4995
4434 772.4223 3085.6601
4435 1029.9048 3086.6926
4444 1034.2384 3099.6934
4448 778.4427 3109.7417
4449 622.9563 3109.7451
4454 1038.9127 3113.7163
4456 1039.2504 3114.7294
4458 1041.5743 3121.7011
4461 1043.9291 3128.7655
4464 1044.5267 3130.5583
4465 1044.5505 3130.6297
4466 784.1738 3132.6661
4476 1049.8976 3146.6710
4477 787.6761 3146.6753
4478 1050.8744 3149.6014
4479 1051.5498 3151.6276
4480 1052.5468 3154.6186
4481 1052.9078 3155.7016
4482 1053.4990 3157.4752
4486 1057.2064 3168.5974
4488 1057.9570 3170.8492
4489 794.4125 3173.6209
4492 795.4323 3177.7001
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