MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3869 837.8306 2510.4700
3872 838.4853 2512.4341
3874 839.1411 2514.4015
3884 843.4360 2527.2862
3885 843.4376 2527.2910
3887 632.8304 2527.2925
3888 843.4396 2527.2970
3889 843.4413 2527.3021
3890 844.4356 2530.2850
3901 511.0789 2550.3581
3902 638.5990 2550.3669
3903 851.1508 2550.4306
3916 852.4412 2554.3018
3917 852.4423 2554.3051
3919 639.5900 2554.3309
3920 852.4595 2554.3567
3928 641.8584 2563.4045
3935 858.4822 2572.4248
3936 859.3960 2575.1662
3938 645.0854 2576.3125
3941 861.1349 2580.3829
3942 861.1417 2580.4033
3947 862.4418 2584.3036
3948 647.0856 2584.3133
3951 647.3414 2585.3365
3954 863.4456 2587.3150
3959 650.3461 2597.3553
3963 867.4707 2599.3903
3965 870.1299 2607.3679
3970 872.1154 2613.3244
3971 872.4324 2614.2754
3972 872.4546 2614.3420
3979 875.4972 2623.4698
3981 657.3501 2625.3713
3986 877.8599 2630.5579
3992 879.4175 2635.2307
3993 879.4182 2635.2328
3999 882.0930 2643.2572
4009 886.1337 2655.3793
4011 664.8536 2655.3853
4013 532.2842 2656.3846
4018 887.4752 2659.4038
4023 891.4565 2671.3477
4033 671.1386 2680.5253
4034 894.8116 2681.4130
4035 895.1874 2682.5404
4038 671.8568 2683.3981
4046 898.4409 2692.3009
4052 899.4938 2695.4596
4058 901.4725 2701.3957
4064 902.4719 2704.3939
4067 903.4843 2707.4311
4070 903.5051 2707.4935
4071 678.1144 2708.4285
4078 907.8215 2720.4427
4080 908.0770 2721.2092
4082 908.4709 2722.3909
4083 908.5213 2722.5421
4084 909.1349 2724.3829
4086 682.8776 2727.4813
4089 546.7038 2728.4826
4091 910.8583 2729.5531
4092 910.8599 2729.5579
4093 910.8623 2729.5651
4094 911.4570 2731.3492
4100 913.5143 2737.5211
4102 916.5322 2746.5748
4103 916.5357 2746.5853
4105 917.5208 2749.5406
4106 917.8493 2750.5261
4107 688.6401 2750.5313
4110 918.1821 2751.5245
4111 689.1384 2752.5245
4116 920.1768 2757.5086
4117 690.3879 2757.5225
4118 920.5322 2758.5748
4121 922.8387 2765.4943
4125 924.4811 2770.4215
4126 693.6141 2770.4273
4128 924.8138 2771.4196
4133 925.5164 2773.5274
4134 925.7965 2774.3677
4138 925.8489 2774.5249
4139 926.1718 2775.4936
4140 926.5056 2776.4950
4146 927.4912 2779.4518
4152 927.4991 2779.4755
4154 927.5002 2779.4788
4155 927.5003 2779.4791
4169 931.5260 2791.5562
4170 931.5325 2791.5757
4173 559.8964 2794.4456
4178 933.8735 2798.5987
4180 934.8436 2801.5090
4181 935.4575 2803.3507
4182 935.4586 2803.3540
4186 936.8434 2807.5084
4195 937.4868 2809.4386
4211 941.1386 2820.3940
4212 941.2029 2820.5869
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