MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2966 643.9722 1928.8948
2973 644.3708 1930.0906
3003 974.0720 1946.1294
3019 984.0653 1966.1160
3021 656.3808 1966.1206
3032 658.0489 1971.1249
3041 990.5398 1979.0650
3044 991.1247 1980.2348
3051 992.5821 1983.1496
3055 663.3553 1987.0441
3057 663.3741 1987.1005
3086 669.7292 2006.1658
3089 503.0507 2008.1737
3091 1005.5621 2009.1096
3108 674.0521 2019.1345
3116 677.0592 2028.1558
3117 677.0602 2028.1588
3121 677.3940 2029.1602
3130 679.0313 2034.0721
3149 681.0670 2040.1792
3158 683.0446 2046.1120
3191 689.3912 2065.1518
3199 1036.6105 2071.2064
3204 692.7225 2075.1457
3225 699.3713 2095.0921
3282 708.4047 2122.1923
3293 710.4197 2128.2373
3299 712.0617 2133.1633
3356 720.7386 2159.1940
3386 725.3568 2173.0486
3391 1088.6158 2175.2170
3394 726.6982 2177.0728
3399 727.0791 2178.2155
3410 728.0617 2181.1633
3411 1091.6044 2181.1942
3412 728.3796 2182.1170
3429 1097.0795 2192.1444
3453 736.0974 2205.2704
3457 737.0519 2208.1339
3466 738.0967 2211.2683
3467 553.8245 2211.2689
3469 555.2928 2217.1421
3480 743.3608 2227.0606
3481 558.3047 2229.1897
3487 744.7479 2231.2219
3499 747.0520 2238.1342
3515 749.0788 2244.2146
3517 1124.1318 2246.2490
3520 1124.6407 2247.2668
3521 750.3870 2248.1392
3532 564.3027 2253.1817
3559 758.4012 2272.1818
3561 758.7462 2273.2168
3580 761.4119 2281.2139
3581 762.1024 2283.2854
3583 762.7798 2285.3176
3584 1144.1478 2286.2810
3588 765.4260 2293.2562
3603 768.0363 2301.0871
3608 576.3133 2301.2241
3643 581.8117 2323.2177
3647 582.0459 2324.1545
3656 777.7271 2330.1595
3659 584.5823 2334.3001
3667 780.8079 2339.4019
3671 1173.6876 2345.3606
3672 782.7953 2345.3641
3673 782.7965 2345.3677
3674 782.7978 2345.3716
3679 784.4668 2350.3786
3681 589.0711 2352.2553
3683 786.3779 2356.1119
3696 787.4102 2359.2088
3704 788.0912 2361.2518
3716 793.7960 2378.3662
3731 795.8058 2384.3956
3734 796.7706 2387.2900
3735 1195.2106 2388.4066
3742 799.4228 2395.2466
3744 600.5979 2398.3625
3752 804.1060 2409.2962
3757 805.8039 2414.3899
3760 806.1154 2415.3244
3775 808.4297 2422.2673
3782 608.5802 2430.2917
3785 813.4455 2437.3147
3788 813.7977 2438.3713
3789 814.3857 2440.1353
3791 611.3711 2441.4553
3798 818.1523 2451.4351
3800 818.7756 2453.3050
3805 820.4162 2458.2268
3818 823.1357 2466.3853
3833 826.4403 2476.2991
3837 827.7963 2480.3671
3838 828.0778 2481.2116
3858 835.4495 2503.3267
3859 836.7528 2507.2366
3865 628.3480 2509.3629
3868 837.8229 2510.4469
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