MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
139 484.3275 483.3202
141 486.3422 485.3349
149 493.2788 492.2715
150 494.2386 493.2313
151 496.2851 495.2778
161 506.3115 505.3042
164 515.7856 514.7783
167 522.2698 521.2625
168 522.2709 521.2636
174 533.1218 532.1145
175 536.3151 535.3078
176 539.3035 538.2962
179 550.8055 549.7982
180 555.1063 554.0990
181 557.0807 556.0734
187 561.8327 560.8254
188 564.3240 563.3167
189 565.2903 564.2830
191 571.0707 570.0634
194 573.8176 572.8103
198 585.8448 584.8375
204 591.8273 590.8200
207 596.3070 595.2997
208 600.0102 599.0029
213 601.6570 600.6497
214 601.9919 600.9846
254 623.3612 622.3539
257 626.3236 625.3163
290 637.8116 636.8043
368 683.8586 682.8513
370 686.8845 685.8772
373 687.8848 686.8775
389 693.8912 692.8839
392 695.3519 694.3446
448 724.3791 723.3718
484 743.3602 742.3529
488 743.8602 742.8529
502 747.9153 746.9080
503 748.8701 747.8628
509 750.4445 749.4372
515 756.3997 755.3924
546 766.4351 765.4278
563 773.9288 772.9215
607 792.4271 791.4198
610 793.4102 792.4029
730 850.9648 849.9575
784 872.9385 871.9312
792 876.4537 875.4464
838 898.4568 897.4495
870 907.1443 906.1370
910 924.4773 923.4700
919 926.0331 925.0258
938 933.4922 932.4849
952 939.9873 938.9800
992 957.5032 956.4959
1057 990.5404 989.5331
1104 1005.9081 1004.9008
1217 1056.5386 1055.5313
1228 1061.2295 1060.2222
1237 1068.2784 1067.2711
1436 582.0435 1162.0724
1716 430.9364 1289.7874
1886 687.1306 1372.2466
1898 692.8215 1383.6284
1913 463.9593 1388.8561
2029 722.4418 1442.8690
2098 738.9452 1475.8758
2215 511.6228 1531.8466
2272 520.6500 1558.9282
2295 786.4019 1570.7892
2307 394.2339 1572.9065
2383 804.4985 1606.9824
2386 805.4742 1608.9338
2389 805.5104 1609.0062
2447 408.7472 1630.9597
2460 818.9893 1635.9640
2468 821.4640 1640.9134
2537 335.4093 1672.0101
2538 419.0099 1672.0105
2548 838.1965 1674.3784
2555 843.9440 1685.8734
2556 562.9909 1685.9509
2581 851.9675 1701.9204
2661 579.0267 1734.0583
2689 581.9797 1742.9173
2693 872.4694 1742.9242
2694 872.4703 1742.9260
2715 587.6906 1760.0500
2750 891.2111 1780.4076
2754 595.6603 1783.9591
2795 905.0214 1808.0282
2796 905.0267 1808.0388
2814 606.6755 1817.0047
2827 456.9959 1823.9545
2867 928.9930 1855.9714
2891 624.3647 1870.0723
2923 631.7158 1892.1256
2928 634.3503 1900.0291
2931 635.3677 1903.0813
2959 641.9727 1922.8963
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