MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
28 345.2270 344.2197
29 347.2305 346.2232
30 351.2393 350.2320
31 353.2546 352.2473
32 355.2100 354.2027
33 355.2708 354.2635
34 358.2457 357.2384
35 359.2202 358.2129
36 359.2414 358.2341
37 359.2415 358.2342
38 359.2416 358.2343
39 359.2419 358.2346
40 359.2419 358.2346
41 359.2425 358.2352
42 359.2665 358.2592
43 359.2668 358.2595
44 360.2261 359.2188
45 361.1842 360.1769
46 361.1986 360.1913
47 361.2002 360.1929
48 361.2209 360.2136
49 367.2343 366.2270
50 371.2419 370.2346
51 371.2542 370.2469
52 373.2198 372.2125
53 373.2833 372.2760
54 374.2299 373.2226
55 374.2411 373.2338
56 375.2256 374.2183
57 375.2373 374.2300
58 376.2643 375.2570
59 385.2576 384.2503
60 385.2593 384.2520
61 385.7490 384.7417
62 386.2404 385.2331
63 386.2415 385.2342
64 387.2729 386.2656
65 387.2732 386.2659
66 387.2740 386.2667
67 388.2567 387.2494
68 388.2570 387.2497
70 389.2394 388.2321
71 389.2400 388.2327
72 389.2531 388.2458
73 393.2487 392.2414
74 395.2359 394.2286
75 395.2640 394.2567
76 398.2463 397.2390
77 399.2307 398.2234
78 401.2884 400.2811
79 401.2890 400.2817
80 401.2892 400.2819
81 401.2899 400.2826
82 402.2475 401.2402
83 402.2479 401.2406
84 402.2482 401.2409
85 403.2316 402.2243
86 404.1904 403.1831
87 404.2517 403.2444
88 407.2278 406.2205
89 409.2195 408.2122
90 416.2633 415.2560
91 416.2635 415.2562
92 416.2883 415.2810
93 416.2883 415.2810
94 416.2884 415.2811
95 416.2903 415.2830
100 418.2668 417.2595
101 421.2587 420.2514
102 423.2946 422.2873
103 425.2389 424.2316
104 426.2477 425.2404
105 428.2895 427.2822
106 429.2465 428.2392
107 430.2790 429.2717
108 430.2791 429.2718
109 430.3041 429.2968
110 430.3045 429.2972
111 430.3047 429.2974
112 430.3053 429.2980
113 432.2590 431.2517
114 432.2592 431.2519
115 440.2636 439.2563
116 444.2950 443.2877
117 444.2952 443.2879
118 444.2961 443.2888
120 446.2996 445.2923
121 448.2573 447.2500
122 451.2691 450.2618
123 456.2947 455.2874
124 458.3106 457.3033
125 458.7524 457.7451
127 460.3147 459.3074
128 460.3156 459.3083
129 471.2370 470.2297
130 471.7737 470.7664
131 472.3522 471.3449
134 474.2686 473.2613
137 476.2115 475.2042
138 479.2634 478.2561
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