| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 821.7771 | 2462.3095 | 2462.3203 | -4.40 | 1 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
NVQQVGGGLLLVSQFTLMAKTDK + Oxidation (M) | |
| 937.5167 | 2809.5283 | 2809.5702 | -14.9 | 1 | 0 | 1 | 1Score > 32 indicates identityScore > 13 indicates homology |
LSSAARVVPVSNVTSTNGWVNLIVITL | |
| 992.5422 | 2974.6048 | 2974.5539 | 17.1 | 1 | 0 | 1 | 1Score > 32 indicates identityScore > 13 indicates homology |
LVGYDQIPITVPPAPVEGLVGLTPDQQR + 3 Deamidated (NQ) | |
| 695.8704 | 2779.4525 | 2779.4109 | 15.0 | 1 | 0 | 0.97 | 1Score > 36 indicates identityScore > 13 indicates homology |
GGVHLSGMGVVNLPSGKPTMALTGGAAER + Oxidation (M) | |
| 662.3688 | 3306.8076 | 3306.7679 | 12.0 | 1 | 0 | 0.92 | 1Score > 30 indicates identityScore > 13 indicates homology |
INITNKITNVVLMGMGEPLLNLSNVVHALR + 2 Deamidated (NQ); 2 Oxidation (M) | |
| 907.4639 | 3625.8265 | 3625.8621 | -9.82 | 1 | 0 | 1 | 1Score > 34 indicates identityScore > 13 indicates homology |
MSLNQAASVNNLVLSPNTTLKDVALNALAGQVDAR + 2 Deamidated (NQ); Oxidation (M) | |
| 693.4153 | 2077.2241 | 2077.2074 | 8.01 | 1 | 0 | 1 | 1Score > 27 indicates identityScore > 13 indicates homology |
FINILSNLLQKGTLIFIK + 3 Deamidated (NQ) | |
| 1016.5309 | 4062.0945 | 4062.0143 | 19.7 | 1 | 0 | 1 | 1Score > 32 indicates identityScore > 13 indicates homology |
FSVRVSLASATAPAEPGQLLNMLFGNSSIQPDVTLADVE + 2 Deamidated (NQ); Oxidation (M) | |
| 554.9855 | 1661.9347 | 1661.9213 | 8.06 | 1 | 0 | 0.92 | 1Score > 35 indicates identityScore > 13 indicates homology |
SHPKPKNISLGDITR | |
| 767.4164 | 2299.2274 | 2299.2035 | 10.4 | 0 | 0 | 0.93 | 1Score > 36 indicates identityScore > 13 indicates homology |
VFLGLDTMHSILLFHDTGVGK | |
| 924.4809 | 923.4736 | 923.4786 | -5.43 | 0 | 0 | 1 | 1Score > 40 indicates identityScore > 13 indicates homology |
TPGVYLMK + Oxidation (M) | |
| 772.0873 | 2313.2401 | 2313.2328 | 3.12 | 1 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
SPPPGLQPNQKTLPTGSDPLLR + Deamidated (NQ) | |
| 591.3217 | 2361.2577 | 2361.2428 | 6.33 | 1 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
NVVIGNDKIVSANTVIDSGGYLI + Deamidated (NQ) | |
| 967.4516 | 4832.2216 | 4832.2706 | -10.1 | 1 | 0 | 1 | 1Score > 29 indicates identityScore > 13 indicates homology |
LAGDQLRMAAYSCDSISHMLQLYYQCTYHGSVTNAAATPLPLK + Deamidated (NQ); Oxidation (M) | |
| 600.9865 | 1799.9377 | 1799.9570 | -10.7 | 1 | 0 | 1 | 1Score > 39 indicates identityScore > 13 indicates homology |
DVVAAPAGVWKNFDALK | |
| 1080.0643 | 2158.1140 | 2158.0728 | 19.1 | 1 | 0 | 1 | 1Score > 38 indicates identityScore > 13 indicates homology |
AVADVYDLLHCIVSDLAER | |
| 809.7483 | 2426.2231 | 2426.2482 | -10.4 | 1 | 0 | 0.99 | 1Score > 38 indicates identityScore > 13 indicates homology |
LVDNGGHLVTVEFDGILPDLFR + Deamidated (NQ) | |
| 920.7717 | 2759.2933 | 2759.3033 | -3.64 | 1 | 0 | 1 | 1Score > 37 indicates identityScore > 13 indicates homology |
MGNSTSLFQRLVNTFIIAMQEPNK + 5 Deamidated (NQ); Oxidation (M) | |
| 943.1613 | 2826.4621 | 2826.4606 | 0.53 | 1 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
DYLVPSRVHHGQFYALPQSPQIFK | |
| 848.4469 | 4237.1981 | 4237.1552 | 10.1 | 1 | 0 | 1 | 1Score > 30 indicates identityScore > 13 indicates homology |
LQVCTVDVGDGAAPVQVVCGAPNARAGLVTVFAPPGTYIPAK + 2 Deamidated (NQ) | |
| 1126.5898 | 4502.3301 | 4502.3525 | -4.98 | 1 | 0 | 1 | 1Score > 30 indicates identityScore > 13 indicates homology |
LFLQNNLIRTLRPGTFGSNLLTLWLFSNNLSTIYPGTFR + 5 Deamidated (NQ) | |
| 803.4037 | 4814.3785 | 4814.2984 | 16.7 | 0 | 0 | 1 | 1Score > 31 indicates identityScore > 13 indicates homology |
QATANGPPMVINQLNLMQSAASGLGMGMGTGTGSGVSVTVTRPPPSPSR + 3 Deamidated (NQ); 2 Oxidation (M) | |
| 1122.5294 | 2243.0442 | 2243.0780 | -15.0 | 1 | 0 | 1 | 1Score > 38 indicates identityScore > 13 indicates homology |
STLNFAKATFDALNQVNVMR + 4 Deamidated (NQ) | |
| 930.8041 | 2789.3905 | 2789.3429 | 17.0 | 0 | 0 | 0.94 | 1Score > 37 indicates identityScore > 13 indicates homology |
QINLDSDLLGDARPFLQDGMTVQLE + 2 Deamidated (NQ) | |
| 1142.6279 | 3424.8619 | 3424.9268 | -19.0 | 1 | 0 | 1 | 1Score > 30 indicates identityScore > 13 indicates homology |
TGSGKTAAFALPILQALMNKPQSLFGLILAPTR | |
| 818.4286 | 4904.5279 | 4904.4753 | 10.7 | 0 | 0 | 1 | 1Score > 29 indicates identityScore > 13 indicates homology |
QLLAGGIINLGLSPANQMPAPQLLGQPTTVNPDFQAGVGIATNATSTAR + 5 Deamidated (NQ); Oxidation (M) | |
| 457.7895 | 913.5644 | 913.5597 | 5.24 | 0 | 0 | 1 | 1Score > 37 indicates identityScore > 13 indicates homology |
AVNIVGITK | |
| 744.3871 | 2973.5193 | 2973.4955 | 7.99 | 0 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
LIFLFVLGGMQGVLGWWMVMSGLTDR + 3 Oxidation (M) | |
| 441.2161 | 1760.8353 | 1760.8064 | 16.4 | 1 | 0 | 1 | 1Score > 40 indicates identityScore > 13 indicates homology |
ATNQQNDIGKQLNNAK + 5 Deamidated (NQ) | |
| 857.1411 | 2568.4015 | 2568.3985 | 1.16 | 1 | 0 | 0.99 | 1Score > 33 indicates identityScore > 13 indicates homology |
AAAQGLMAGINAVLALKNQDPFILK + 2 Deamidated (NQ) | |
| 778.3471 | 1554.6796 | 1554.6870 | -4.72 | 1 | 0 | 0.99 | 1Score > 38 indicates identityScore > 13 indicates homology |
SNNATSSTSQGTRSR + 2 Deamidated (NQ) | |
| 925.8446 | 2774.5120 | 2774.4888 | 8.35 | 1 | 0 | 1 | 1Score > 32 indicates identityScore > 13 indicates homology |
VLFVGMISNSKPVVSNILNRVTPGSK + 3 Deamidated (NQ); Oxidation (M) | |
| 927.4942 | 2779.4608 | 2779.4504 | 3.72 | 0 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
ALPPGQGLGPAEPPGLPQLQSLNLSGNR + 2 Deamidated (NQ) | |
| 641.5639 | 3202.7831 | 3202.8329 | -15.5 | 1 | 0 | 1 | 1Score > 29 indicates identityScore > 13 indicates homology |
LDAVLASGDAIRNHPLYGTVLVLVLLGALTK + Deamidated (NQ) | |
| 837.3953 | 2509.1641 | 2509.2053 | -16.4 | 1 | 0 | 1 | 1Score > 37 indicates identityScore > 13 indicates homology |
QCIDLPDNHPAMASKTVLQTNR + Deamidated (NQ) | |
| 699.3734 | 2095.0984 | 2095.0983 | 0.036 | 1 | 0 | 1 | 1Score > 38 indicates identityScore > 13 indicates homology |
ASVMSPAVNLSIQNVKVAHK + 3 Deamidated (NQ) | |
| 746.7250 | 2237.1532 | 2237.1369 | 7.29 | 1 | 0 | 1 | 1Score > 38 indicates identityScore > 13 indicates homology |
DFFALLEVDYDPQVVNVVR | |
| 775.9180 | 3099.6429 | 3099.6605 | -5.67 | 1 | 0 | 1 | 1Score > 33 indicates identityScore > 13 indicates homology |
VQLLDALDTQIATVGFDLVQWRTQLVR + 2 Deamidated (NQ) | |
| 757.6017 | 3782.9721 | 3783.0083 | -9.56 | 1 | 0 | 0.99 | 1Score > 32 indicates identityScore > 13 indicates homology |
IHNPAVFTWPPWQAGILARNLVPMVATVQGQNLK + Deamidated (NQ); Oxidation (M) | |
| 557.0806 | 2780.3666 | 2780.3756 | -3.22 | 1 | 0 | 1 | 1Score > 38 indicates identityScore > 13 indicates homology |
NRPDYVNIQLYLSQTDGIQKIIGE + 4 Deamidated (NQ) | |
| 916.4822 | 6408.3245 | 6408.2908 | 5.25 | 1 | 0 | 1 | 1Score > 26 indicates identityScore > 13 indicates homology |
VTQGIPLSSGMILLTWQKIAPLSVLYQISPSINTNLLMTMATMSVLIGGWGGLNQTQLR + 6 Deamidated (NQ); 3 Oxidation (M) | |
| 463.6542 | 2313.2346 | 2313.1901 | 19.2 | 1 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
FIKMGYPILGICNGFQVLVE + Oxidation (M) | |
| 604.8367 | 2415.3177 | 2415.2798 | 15.7 | 1 | 0 | 1 | 1Score > 34 indicates identityScore > 13 indicates homology |
VGNNLTAQLFWKPVVNKLTSGE + Deamidated (NQ) | |
| 683.8712 | 2731.4557 | 2731.4942 | -14.1 | 1 | 0 | 1 | 1Score > 34 indicates identityScore > 13 indicates homology |
QGLTVLAVMAGIYAVILGVTTAAESGAR | |
| 710.3782 | 2837.4837 | 2837.4964 | -4.46 | 1 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
TFQDRTPVTIKPYYVTLDLIDPSR | |
| 785.3925 | 3137.5409 | 3137.5215 | 6.17 | 1 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
AMGVSLVMHPHNPYVPTSHANVRFFIAE + Deamidated (NQ); Oxidation (M) | |
| 581.6678 | 1741.9816 | 1741.9938 | -7.01 | 1 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
QLAGLLLRITDGTVSGK + Deamidated (NQ) | |
| 820.4190 | 3277.6469 | 3277.6120 | 10.7 | 1 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
NYAYLGLKPVFIFFDEYVAFMDLLDMK + Oxidation (M) | |
| 770.8861 | 3079.5153 | 3079.5132 | 0.70 | 1 | 0 | 1 | 1Score > 37 indicates identityScore > 13 indicates homology |
SRYNVDVAQLPGADLSQLTSAQAASLMQK + 2 Deamidated (NQ); Oxidation (M) | |
| 1046.5634 | 3136.6684 | 3136.6334 | 11.2 | 1 | 0 | 1 | 1Score > 33 indicates identityScore > 13 indicates homology |
LGIMFNPMLASLAMSLSSVSVVLNAQRLR + 3 Deamidated (NQ); Oxidation (M) | |
| 1006.2841 | 4021.1073 | 4021.0526 | 13.6 | 1 | 0 | 1 | 1Score > 31 indicates identityScore > 13 indicates homology |
RSLQNVANYLIGSLAVTDLMVSVLVLPMAAQNQVLNK + 7 Deamidated (NQ); 2 Oxidation (M) | |
| 877.4585 | 2629.3537 | 2629.3567 | -1.16 | 1 | 0 | 1 | 1Score > 37 indicates identityScore > 13 indicates homology |
NVLAVAIGVADGMQLGMNARAGLITR + 3 Deamidated (NQ); Oxidation (M) | |
| 1008.8439 | 3023.5099 | 3023.4546 | 18.3 | 1 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
VASSIFIPFSVGGGINTVDDMRDALNAGAE + Deamidated (NQ) | |
| 1115.5793 | 4458.2881 | 4458.2127 | 16.9 | 1 | 0 | 1 | 1Score > 31 indicates identityScore > 13 indicates homology |
HSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLR + 3 Deamidated (NQ); Oxidation (M) | |
| 641.0667 | 2560.2377 | 2560.2243 | 5.24 | 1 | 0 | 1 | 1Score > 38 indicates identityScore > 13 indicates homology |
AADAFVLWGSNMVEMHPILWTR + Deamidated (NQ); Oxidation (M) | |
| 1030.8977 | 3089.6713 | 3089.6278 | 14.1 | 1 | 0 | 1 | 1Score > 32 indicates identityScore > 13 indicates homology |
TATMALSTLAALIQISGAKNITLSPDQTSR + Deamidated (NQ); Oxidation (M) | |
| 932.4977 | 3725.9617 | 3725.9702 | -2.29 | 1 | 0 | 1 | 1Score > 32 indicates identityScore > 13 indicates homology |
AMLNVDKYLDDAYIAGLTHVTIIHGVGTGVLSAGLK + Deamidated (NQ) | |
| 808.4605 | 2422.3597 | 2422.3229 | 15.2 | 1 | 0 | 1 | 1Score > 31 indicates identityScore > 13 indicates homology |
VLVHQRIAPVFLPMLAQAMQK + Deamidated (NQ); 2 Oxidation (M) | |
| 899.0507 | 4490.2171 | 4490.2146 | 0.55 | 1 | 0 | 1 | 1Score > 31 indicates identityScore > 13 indicates homology |
AQSFGPAVASGLMCGDGLWALPQAVLSLVNVNPPLCMKFLSR + 3 Deamidated (NQ); Oxidation (M) | |
| 793.4709 | 3169.8545 | 3169.8155 | 12.3 | 1 | 0 | 1 | 1Score > 23 indicates identityScore > 13 indicates homology |
LGTNIIAILLIIYGFSLVSGISVRQFFSK + Deamidated (NQ) | |
| 976.2560 | 3900.9949 | 3900.9574 | 9.60 | 0 | 0 | 1 | 1Score > 33 indicates identityScore > 13 indicates homology |
QVVQFVLADASSAGGPHVAFANGVFVDASLPLKPSFQE + 2 Deamidated (NQ) | |
| 678.3904 | 1354.7662 | 1354.7569 | 6.91 | 0 | 0 | 1 | 1Score > 37 indicates identityScore > 13 indicates homology |
VVAVVNNDVITGR | |
| 790.4397 | 1578.8648 | 1578.8405 | 15.4 | 1 | 0 | 1 | 1Score > 37 indicates identityScore > 13 indicates homology |
NALTYQYISLHKK + Deamidated (NQ) | |
| 793.0729 | 2376.1969 | 2376.2359 | -16.4 | 1 | 0 | 1 | 1Score > 39 indicates identityScore > 13 indicates homology |
RVMQLFGLVNTLLANDPTSLR + 3 Deamidated (NQ); Oxidation (M) | |
| 699.3913 | 2793.5361 | 2793.5323 | 1.35 | 1 | 0 | 1 | 1Score > 32 indicates identityScore > 13 indicates homology |
HAALIGIAQLFALIPGASRSGVTMTAAR + Deamidated (NQ) | |
| 983.8572 | 2948.5498 | 2948.5239 | 8.78 | 1 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
CSVNIEVDLIVGADMVVLIVLTQFQR + 2 Deamidated (NQ); Oxidation (M) | |
| 1168.2627 | 3501.7663 | 3501.7092 | 16.3 | 1 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
QFNQVPLYIRPQSFFQTNPQVAAELYATAR + 5 Deamidated (NQ) | |
| 555.0300 | 2216.0909 | 2216.1338 | -19.4 | 1 | 0 | 1 | 1Score > 39 indicates identityScore > 13 indicates homology |
WGTVAFASSLDQAGPIARDVR | |
| 1038.8549 | 3113.5429 | 3113.5063 | 11.8 | 0 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
GGHAAYPHLANDMVVAASAFVGQMQTIISR + 2 Deamidated (NQ) | |
| 1150.5856 | 3448.7350 | 3448.7613 | -7.64 | 1 | 0 | 1 | 1Score > 35 indicates identityScore > 13 indicates homology |
ILVSATNAVLSDGSSLPASVENGTIFAGFPSADLK + Deamidated (NQ) | |
| 474.2925 | 473.2852 | 473.2849 | 0.59 | 0 | 0 | 1 | 1Score > 43 indicates identityScore > 13 indicates homology |
LITGA | |
| 718.8904 | 2871.5325 | 2871.5719 | -13.7 | 1 | 0 | 1 | 1Score > 34 indicates identityScore > 13 indicates homology |
TPAVSLQLVGPGHYVARPLQDNALSRL | |
| 724.3775 | 2893.4809 | 2893.4506 | 10.5 | 1 | 0 | 1 | 1Score > 36 indicates identityScore > 13 indicates homology |
LAYCLQKCNVPLFVQHDAAQLYLK + 2 Deamidated (NQ) | |
| 303.1784 | 302.1711 | ||||||||
| 303.1787 | 302.1714 | ||||||||
| 303.1788 | 302.1715 | ||||||||
| 303.1789 | 302.1716 | ||||||||
| 303.1790 | 302.1717 | ||||||||
| 303.2039 | 302.1966 | ||||||||
| 304.1627 | 303.1554 | ||||||||
| 305.0120 | 304.0047 | ||||||||
| 305.1829 | 304.1756 | ||||||||
| 307.6800 | 306.6727 | ||||||||
| 309.0330 | 308.0257 | ||||||||
| 317.1947 | 316.1874 | ||||||||
| 317.2193 | 316.2120 | ||||||||
| 317.2194 | 316.2121 | ||||||||
| 317.2198 | 316.2125 | ||||||||
| 322.1883 | 321.1810 | ||||||||
| 322.1898 | 321.1825 | ||||||||
| 323.8394 | 322.8321 | ||||||||
| 331.2351 | 330.2278 | ||||||||
| 331.2352 | 330.2279 | ||||||||
| 333.1898 | 332.1825 | ||||||||
| 333.2144 | 332.2071 | ||||||||
| 337.2601 | 336.2528 | ||||||||
| 345.2258 | 344.2185 | ||||||||
| 345.2259 | 344.2186 | ||||||||
| 345.2259 | 344.2186 | ||||||||
| 345.2260 | 344.2187 | ||||||||
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