MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
856 451.7460 901.4774 901.4756 2.01 1 26 0.32 +1Score > 43 indicates identity
Score > 34 indicates homology		 QVVNALEK + 2 Deamidated (NQ)
1928 699.8873 1397.7600 1397.7514 6.18 1 26 0.26 +1Score > 39 indicates identity
Score > 33 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
460 365.2303 728.4460 728.4432 3.90 0 26 0.58 +1Score > 42 indicates identity
Score > 36 indicates homology		 LAANLVK + Deamidated (NQ)
840 449.7790 897.5434 897.5396 4.31 0 26 0.15 +1Score > 36 indicates identity
Score > 30 indicates homology		 QILLGAQR
915 463.2811 924.5476 924.5392 9.09 0 26 0.53 +1Score > 36 indicates identity GLQIAAVPR + Deamidated (NQ)
1223 529.7945 1057.5744 1057.5590 14.6 0 26 0.28 +1Score > 42 indicates identity
Score > 33 indicates homology		 IAVSPMLGNR + Deamidated (NQ)
1479 1181.7217 1180.7144 1180.7067 6.52 1 26 0.25 +1Score > 32 indicates identity DVVPVLIEGLK
453 728.4332 727.4259 727.4229 4.22 0 26 0.99 +1Score > 39 indicates identity
Score > 38 indicates homology		 VGTPQVK
1580 614.8386 1227.6626 1227.6459 13.7 1 26 0.88 +1Score > 40 indicates identity
Score > 38 indicates homology		 QAQQLLELQR + 2 Deamidated (NQ)
240 615.3862 614.3789 614.3752 6.14 0 26 0.76 +1Score > 42 indicates identity
Score > 37 indicates homology		 QGVIAK
1929 699.8878 1397.7610 1397.7514 6.90 1 26 0.23 +1Score > 39 indicates identity
Score > 32 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
1699 642.8693 1283.7240 1283.7449 -16.2 0 26 0.66 +1Score > 39 indicates identity
Score > 36 indicates homology		 LVDTAALVVDLR
646 404.7156 807.4166 807.4126 4.96 0 26 0.17 +1Score > 42 indicates identity
Score > 30 indicates homology		 YAAVDAAK
2615 857.9727 1713.9308 1713.9553 -14.3 1 26 0.14 +1Score > 38 indicates identity
Score > 30 indicates homology		 EALADIDAILFVVPTK
896 459.7629 917.5112 917.4970 15.5 1 26 0.72 +1Score > 41 indicates identity
Score > 37 indicates homology		 VIAAYPER
327 332.1838 662.3530 662.3500 4.59 0 26 0.24 +1Score > 40 indicates identity
Score > 32 indicates homology		 FGNVAR
1403 575.7933 1149.5720 1149.5666 4.76 1 26 0.62 +1Score > 42 indicates identity
Score > 36 indicates homology		 AQIDFSAEIR + Deamidated (NQ)
182 558.3636 557.3563 557.3537 4.74 0 25 0.56 +1Score > 40 indicates identity
Score > 35 indicates homology		 AGAVLK
305 647.3509 646.3436 646.3398 5.87 0 25 0.98 +1Score > 41 indicates identity
Score > 38 indicates homology		 GTISNR
928 465.2236 928.4326 928.4290 3.91 1 25 0.085 +1Score > 39 indicates identity
Score > 27 indicates homology		 YDALYRE
511 377.2175 752.4204 752.4181 3.15 0 25 0.14 +1Score > 38 indicates identity
Score > 29 indicates homology		 ALDVHAK
1097 502.2783 1002.5420 1002.5346 7.47 1 25 0.3 +1Score > 42 indicates identity
Score > 33 indicates homology		 VDLSTLAER
344 336.7129 671.4112 671.4078 5.08 1 25 0.82 +1Score > 42 indicates identity
Score > 37 indicates homology		 RVQIR + Deamidated (NQ)
2351 793.9269 1585.8392 1585.8311 5.14 1 25 0.13 +1Score > 40 indicates identity
Score > 29 indicates homology		 QAITNPNTIQSIKR + 3 Deamidated (NQ)
273 316.7036 631.3926 631.3905 3.45 0 25 0.23 +1Score > 31 indicates identity ITGTIK
1930 699.8881 1397.7616 1397.7514 7.33 1 25 0.23 +1Score > 39 indicates identity
Score > 32 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
2158 755.9445 1509.8744 1509.8654 5.99 0 25 0.2 +1Score > 34 indicates identity
Score > 31 indicates homology		 LIVLTDTPGILLNE
1028 486.7738 971.5330 971.5400 -7.13 1 25 0.24 +1Score > 42 indicates identity
Score > 32 indicates homology		 LIQEVAGSR
873 454.7472 907.4798 907.4651 16.3 1 25 0.38 +1Score > 41 indicates identity
Score > 34 indicates homology		 QINEYIK + Deamidated (NQ)
1314 371.5471 1111.6195 1111.6138 5.09 0 25 0.78 +1Score > 38 indicates identity
Score > 37 indicates homology		 LADAHLFIGR
923 464.2749 926.5352 926.5185 18.1 0 25 0.95 +1Score > 38 indicates identity
Score > 37 indicates homology		 LASSPSPLR
1280 547.2819 1092.5492 1092.5451 3.76 0 25 0.25 +1Score > 42 indicates identity
Score > 32 indicates homology		 IGAGFDSVAQK + Deamidated (NQ)
500 747.4810 746.4737 746.4803 -8.77 1 25 0.3 +1Score > 32 indicates identity KFLAIR
679 416.2109 830.4072 830.4134 -7.38 1 25 0.52 +1Score > 42 indicates identity
Score > 35 indicates homology		 EIDQAAGK
1858 455.2234 1362.6484 1362.6449 2.55 1 25 0.18 +1Score > 41 indicates identity
Score > 30 indicates homology		 MLNQLDNLTER + Deamidated (NQ); Oxidation (M)
788 437.7504 873.4862 873.4920 -6.54 1 25 0.8 +1Score > 42 indicates identity
Score > 37 indicates homology		 AELTIATR
215 602.3527 601.3454 601.3435 3.19 0 25 0.31 +1Score > 45 indicates identity
Score > 32 indicates homology		 LSAQIA
1376 1139.6462 1138.6389 1138.6387 0.23 0 25 0.55 +1Score > 37 indicates identity
Score > 35 indicates homology		 FGAFVTILSGK
501 374.2442 746.4738 746.4691 6.42 0 25 0.13 +1Score > 32 indicates identity
Score > 29 indicates homology		 AGFVILK
494 374.2435 746.4724 746.4691 4.55 0 25 0.21 +1Score > 33 indicates identity
Score > 31 indicates homology		 AGFVILK
542 382.7336 763.4526 763.4480 6.13 0 25 0.56 +1Score > 35 indicates identity YIDIIK
144 488.2605 487.2532 487.2503 6.02 0 25 0.46 +1Score > 36 indicates identity
Score > 34 indicates homology		 AGQGR
491 373.7269 745.4392 745.4334 7.87 0 25 0.66 +1Score > 39 indicates identity
Score > 36 indicates homology		 QSASLLK
1935 350.9334 1399.7045 1399.7096 -3.63 1 25 0.43 +1Score > 41 indicates identity
Score > 34 indicates homology		 SLSTGNSKGFFVR + Deamidated (NQ)
1189 518.2986 1034.5826 1034.6012 -17.9 1 25 0.2 +1Score > 38 indicates identity
Score > 30 indicates homology		 IEIYIVGTK
520 379.2336 756.4526 756.4494 4.33 1 25 0.52 +1Score > 41 indicates identity
Score > 34 indicates homology		 AGEVILR
1916 695.8729 1389.7312 1389.7252 4.35 0 25 0.86 +1Score > 40 indicates identity
Score > 37 indicates homology		 QFLQQLSQLQR + 2 Deamidated (NQ)
314 329.6994 657.3842 657.3810 4.97 0 25 0.71 +1Score > 43 indicates identity
Score > 36 indicates homology		 LVVDGR
2599 854.9540 1707.8934 1707.8931 0.23 1 25 0.23 +1Score > 39 indicates identity
Score > 31 indicates homology		 LLLVDDALQELIHSE + Deamidated (NQ)
238 307.7162 613.4178 613.4163 2.56 0 25 0.22 +1Score > 36 indicates identity
Score > 30 indicates homology		 IIAGIK
555 386.7152 771.4158 771.4239 -10.4 0 25 0.21 +1Score > 41 indicates identity
Score > 30 indicates homology		 NVVDLGR
1771 660.8287 1319.6428 1319.6622 -14.7 1 25 0.37 +1Score > 41 indicates identity
Score > 33 indicates homology		 INQQAFQWKR + 2 Deamidated (NQ)
1536 606.3449 1210.6752 1210.6822 -5.75 1 25 0.61 +1Score > 38 indicates identity
Score > 35 indicates homology		 QSILLRYYR
1272 544.3104 1086.6062 1086.6073 -1.00 0 24 0.18 +1Score > 40 indicates identity
Score > 30 indicates homology		 VPIQIQNFK + Deamidated (NQ)
1529 604.2859 1206.5572 1206.5662 -7.45 1 24 0.15 +1Score > 41 indicates identity
Score > 29 indicates homology		 NLNGLRTMNR + 3 Deamidated (NQ); Oxidation (M)
759 431.2393 860.4640 860.4603 4.32 0 24 0.6 +1Score > 42 indicates identity
Score > 35 indicates homology		 TATIVNNK + Deamidated (NQ)
482 370.7257 739.4368 739.4341 3.76 0 24 0.57 +1Score > 35 indicates identity
Score > 34 indicates homology		 AVAVAGPR
635 403.2319 804.4492 804.4453 4.85 1 24 0.21 +1Score > 41 indicates identity
Score > 30 indicates homology		 KSQSSLR
1128 1014.5766 1013.5693 1013.5506 18.5 0 24 0.63 +1Score > 41 indicates identity
Score > 35 indicates homology		 DVVANLLDR
1210 525.8085 1049.6024 1049.6094 -6.60 1 24 0.58 +1Score > 36 indicates identity
Score > 34 indicates homology		 LHGNLLRAR + Deamidated (NQ)
1520 602.7968 1203.5790 1203.5771 1.58 1 24 0.86 +1Score > 41 indicates identity
Score > 36 indicates homology		 WLQEQGSDLK + Deamidated (NQ)
1562 610.3036 1218.5926 1218.5728 16.3 0 24 0.94 +1Score > 42 indicates identity
Score > 37 indicates homology		 ASNAQLLTNQR + 4 Deamidated (NQ)
649 405.6943 809.3740 809.3589 18.7 1 24 0.55 +1Score > 40 indicates identity
Score > 34 indicates homology		 DAMKNSK + Deamidated (NQ); Oxidation (M)
1581 614.8388 1227.6630 1227.6459 14.0 1 24 0.4 +1Score > 40 indicates identity
Score > 33 indicates homology		 QAQQLLELQR + 2 Deamidated (NQ)
2610 856.9712 1711.9278 1711.9581 -17.7 1 24 0.21 +1Score > 38 indicates identity
Score > 30 indicates homology		 DNITVTVLGVKGNQVR
133 473.3115 472.3042 472.3009 6.97 0 24 0.99 +1Score > 45 indicates identity
Score > 37 indicates homology		 IGGVK
1575 613.8488 1225.6830 1225.6779 4.22 1 24 0.31 +1Score > 38 indicates identity
Score > 32 indicates homology		 QLVAELQGNVR
1236 534.3287 1066.6428 1066.6499 -6.57 1 24 0.22 +1Score > 34 indicates identity
Score > 30 indicates homology		 LIAGLGNPGKK
1921 695.8881 1389.7616 1389.7616 0.048 1 24 0.32 +1Score > 39 indicates identity
Score > 32 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
451 364.2223 726.4300 726.4276 3.42 1 24 0.76 +1Score > 36 indicates identity
Score > 35 indicates homology		 IPQEIK
507 375.6945 749.3744 749.3629 15.4 1 24 0.89 +1Score > 42 indicates identity
Score > 36 indicates homology		 MEVDIK + Oxidation (M)
2247 387.9581 1547.8033 1547.8043 -0.62 0 24 0.59 +1Score > 40 indicates identity
Score > 34 indicates homology		 ITDTNITSIDSLVR + Deamidated (NQ)
413 351.7071 701.3996 701.4072 -10.7 0 24 0.57 +1Score > 43 indicates identity
Score > 34 indicates homology		 IISNVR + Deamidated (NQ)
1689 640.3150 1278.6154 1278.5979 13.7 1 24 0.093 +1Score > 41 indicates identity
Score > 26 indicates homology		 QLTIDELFNGE + Deamidated (NQ)
1463 586.8068 1171.5990 1171.6197 -17.6 0 24 0.6 +1Score > 41 indicates identity
Score > 34 indicates homology		 GSVGVGQPTLTR + Deamidated (NQ)
2183 506.9531 1517.8375 1517.8202 11.4 1 24 0.27 +1Score > 38 indicates identity
Score > 31 indicates homology		 VKFNVINTAVQGAR + 2 Deamidated (NQ)
533 381.2076 760.4006 760.3967 5.26 1 24 0.83 +1Score > 44 indicates identity
Score > 35 indicates homology		 AQTEALK + Deamidated (NQ)
1407 384.5414 1150.6024 1150.6169 -12.6 1 24 0.16 +1Score > 40 indicates identity
Score > 28 indicates homology		 LCLVSKQFR + Deamidated (NQ)
538 382.2099 762.4052 762.4024 3.71 0 24 0.22 +1Score > 43 indicates identity
Score > 30 indicates homology		 FANGLNK
1965 707.9152 1413.8158 1413.8132 1.85 1 24 0.26 +1Score > 35 indicates identity
Score > 30 indicates homology		 LLFLIPPEHAHK
638 403.2521 804.4896 804.4857 4.85 0 24 0.45 +1Score > 35 indicates identity
Score > 33 indicates homology		 YLQILR
890 458.7651 915.5156 915.5025 14.3 0 24 0.86 +1Score > 42 indicates identity
Score > 35 indicates homology		 AIQQASVAK + Deamidated (NQ)
1827 448.6021 1342.7845 1342.7820 1.84 1 24 0.51 +1Score > 35 indicates identity
Score > 33 indicates homology		 TSLLRAVQQVVK + 2 Deamidated (NQ)
587 394.2606 786.5066 786.5004 8.01 0 24 0.47 +1Score > 33 indicates identity FLPAVIK
712 422.7488 843.4830 843.4814 1.95 0 24 0.25 +1Score > 43 indicates identity
Score > 30 indicates homology		 IQSGAVIR + Deamidated (NQ)
2397 538.2916 1611.8530 1611.8580 -3.12 1 24 0.17 +1Score > 39 indicates identity
Score > 28 indicates homology		 LSARPGNEVNLLDSK
949 470.3009 938.5872 938.5913 -4.28 1 23 0.37 +1Score > 32 indicates identity
Score > 32 indicates homology		 LLARIEPK
2364 531.9357 1592.7853 1592.7617 14.8 1 23 0.22 +1Score > 41 indicates identity
Score > 29 indicates homology		 GTLSNWQKQMLDR + Deamidated (NQ); Oxidation (M)
3015 980.0213 1958.0280 1958.0223 2.92 1 23 0.35 +1Score > 39 indicates identity
Score > 31 indicates homology		 FLGIFNTGLKMFPDLTK + Deamidated (NQ); Oxidation (M)
3364 721.3816 2161.1230 2161.1406 -8.15 1 23 0.06 +1Score > 38 indicates identity
Score > 24 indicates homology		 FTVLDDVIEDVVTILNNLK + 2 Deamidated (NQ)
216 602.3533 601.3460 601.3435 4.16 0 23 0.76 +1Score > 45 indicates identity
Score > 35 indicates homology		 SITGPK
757 430.7481 859.4816 859.4949 -15.4 1 23 0.95 +1Score > 42 indicates identity
Score > 36 indicates homology		 LKMNLNK
1388 571.8279 1141.6412 1141.6343 6.08 0 23 0.94 +1Score > 40 indicates identity
Score > 36 indicates homology		 GLTGPTTVPATK
459 365.2232 728.4318 728.4181 18.9 0 23 0.79 +1Score > 44 indicates identity
Score > 35 indicates homology		 QDVIVR
2040 726.3389 1450.6632 1450.6861 -15.8 1 23 0.47 +1Score > 40 indicates identity
Score > 33 indicates homology		 TTAEVMVAVDVTSE
1008 480.7935 959.5724 959.5586 14.4 1 23 0.5 +1Score > 36 indicates identity
Score > 33 indicates homology		 IIVNAKMR + Oxidation (M)
1348 564.8069 1127.5992 1127.6186 -17.2 1 23 0.55 +1Score > 41 indicates identity
Score > 33 indicates homology		 LVELADQALR + Deamidated (NQ)
412 351.7065 701.3984 701.4072 -12.5 0 23 0.4 +1Score > 43 indicates identity
Score > 32 indicates homology		 VGAGATVK
641 404.2126 806.4106 806.3956 18.6 1 23 0.28 +1Score > 41 indicates identity
Score > 30 indicates homology		 MSELVGR + Oxidation (M)
775 435.2421 868.4696 868.4654 4.88 1 23 0.87 +1Score > 37 indicates identity
Score > 35 indicates homology		 GPASPLEAK
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