MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4517 1074.8842 3221.6308 3221.5669 19.8 1 4 0.64 +1Score > 36 indicates identity
Score > 14 indicates homology
NAEVQYAGPSGHIFDGLNFFILTDSNAPVK + Deamidated (NQ)
1968 708.4017 1414.7888 1414.7656 16.4 0 4 0.73 +1Score > 38 indicates identity
Score > 15 indicates homology
FHCVRPFLALR
3054 663.3523 1987.0351 1987.0738 -19.5 0 4 0.61 +1Score > 39 indicates identity
Score > 14 indicates homology
GVNALDAAILAYNNLSVLR + Deamidated (NQ)
1039 975.4948 974.4875 974.4920 -4.63 1 4 0.8 +1Score > 42 indicates identity
Score > 15 indicates homology
QVTGSLLEE
1352 1130.6194 1129.6121 1129.5992 11.4 1 4 0.58 +1Score > 41 indicates identity
Score > 14 indicates homology
GGFDINVPRR
2835 915.4763 1828.9380 1828.9539 -8.66 1 4 0.9 +1Score > 40 indicates identity
Score > 16 indicates homology
MQAGHITVKSMLNILR + 2 Deamidated (NQ); Oxidation (M)
3050 662.0555 1983.1447 1983.1193 12.8 1 4 1 +1Score > 30 indicates identity
Score > 16 indicates homology
VPWGIVLLLGGGFALAKGSE
4348 990.1824 2967.5254 2967.4914 11.4 0 4 0.57 +1Score > 36 indicates identity
Score > 14 indicates homology
YMHLNIPFYGVNLGSLGFLMNPLDIK + 2 Deamidated (NQ)
289 319.2114 636.4082 636.4071 1.77 0 4 0.54 +1Score > 27 indicates identity
Score > 13 indicates homology
IIVHR
2532 557.9250 1670.7532 1670.7821 -17.3 1 4 0.87 +1Score > 39 indicates identity
Score > 15 indicates homology
LQAEIDMQSGILHSE + Deamidated (NQ)
3238 526.2876 2101.1213 2101.1037 8.38 0 4 0.58 +1Score > 37 indicates identity
Score > 14 indicates homology
WLSPIIVTFFSPAHPNFK + Deamidated (NQ)
4579 836.4609 3341.8145 3341.8210 -1.93 1 4 0.65 +1Score > 31 indicates identity
Score > 14 indicates homology
LNHNHMLLWLLLIGTVPVGIAGLLFNKQAE + 2 Deamidated (NQ); Oxidation (M)
642 807.4404 806.4331 806.4286 5.55 0 4 0.93 +1Score > 42 indicates identity
Score > 16 indicates homology
GISFQVR + Deamidated (NQ)
2817 910.0097 1818.0048 1817.9808 13.2 1 4 0.88 +1Score > 36 indicates identity
Score > 15 indicates homology
TLQTLEIQLQSLMATK + Deamidated (NQ)
3627 771.4017 2311.1833 2311.2059 -9.80 1 4 0.79 +1Score > 38 indicates identity
Score > 15 indicates homology
KIINTANLNHLNIFDLITNE + 2 Deamidated (NQ)
4598 678.3455 3386.6911 3386.7180 -7.94 1 4 0.56 +1Score > 35 indicates identity
Score > 13 indicates homology
TNQGGLLQVRCDGVAVIFAHNQIFIGLSNIK + 5 Deamidated (NQ)
4074 904.1047 2709.2923 2709.2929 -0.22 1 3 1 +1Score > 38 indicates identity
Score > 16 indicates homology
ATHSTMEHQLGHLPPINAASSPQQR + 2 Deamidated (NQ)
4236 948.5493 2842.6261 2842.5891 13.0 1 3 1 +1Score > 26 indicates identity
Score > 16 indicates homology
IIITWSRGSTIIPIMIGHTIAIHNGK + Deamidated (NQ)
2382 402.7325 1606.9009 1606.9043 -2.11 1 3 1 +1Score > 35 indicates identity
Score > 16 indicates homology
RAGDVIPQVVQVVVE
3273 1061.0511 2120.0876 2120.0870 0.29 1 3 0.65 +1Score > 39 indicates identity
Score > 14 indicates homology
RGALQAIDQMSLFRPLCK + Deamidated (NQ); Oxidation (M)
4050 898.4625 2692.3657 2692.3604 1.96 1 3 1 +1Score > 37 indicates identity
Score > 16 indicates homology
VLIVNMWEQQIGLYQGNNMALLK + 2 Deamidated (NQ); Oxidation (M)
3337 717.0198 2148.0376 2148.0238 6.40 1 3 0.89 +1Score > 39 indicates identity
Score > 15 indicates homology
MAAPMNGQVCVVTGASRGIGR + Deamidated (NQ); Oxidation (M)
4244 951.1454 2850.4144 2850.3739 14.2 0 3 0.61 +1Score > 37 indicates identity
Score > 14 indicates homology
IVTGQAGDGITMQGSQLAQMLGLVQGSR + 3 Deamidated (NQ); 2 Oxidation (M)
4427 771.1393 3080.5281 3080.4972 10.0 1 3 0.79 +1Score > 36 indicates identity
Score > 15 indicates homology
SRYNVDVAQLPGADLSQLTSAQAASLMQK + 3 Deamidated (NQ); Oxidation (M)
4946 835.4465 5006.6353 5006.6535 -3.63 0 3 1 +1Score > 28 indicates identity
Score > 16 indicates homology
WTTINVPLVVSQTPGADGATVTMTVQNILDQLCPGLLALGLTLLMVR + Deamidated (NQ); Oxidation (M)
2106 741.9149 1481.8152 1481.7878 18.5 1 3 0.97 +1Score > 38 indicates identity
Score > 16 indicates homology
AIVKATAYFNDAAK
3244 1055.0200 2108.0254 2108.0459 -9.72 1 3 0.97 +1Score > 40 indicates identity
Score > 16 indicates homology
GGFQALKGIINSILAQSCTE + 2 Deamidated (NQ)
3442 733.3959 2197.1659 2197.1776 -5.36 1 3 0.77 +1Score > 37 indicates identity
Score > 15 indicates homology
SVGAAAVMPLGSPIGTNKGLQTK + Deamidated (NQ)
4795 1042.2515 4164.9769 4164.9457 7.49 1 3 0.68 +1Score > 33 indicates identity
Score > 14 indicates homology
LTIPPQNGDFAMEPNYLHIWPRDTFMMIALPNMNK + 2 Deamidated (NQ); 3 Oxidation (M)
3418 730.0238 2187.0496 2187.0483 0.56 1 3 0.82 +1Score > 39 indicates identity
Score > 15 indicates homology
YNNFFQKNNSNNLLNIVK + 4 Deamidated (NQ)
1694 640.8897 1279.7648 1279.7499 11.6 1 3 0.91 +1Score > 33 indicates identity
Score > 16 indicates homology
QLLTHALKLNK + 2 Deamidated (NQ)
3898 850.4295 2548.2667 2548.2896 -9.01 1 3 0.97 +1Score > 38 indicates identity
Score > 16 indicates homology
TQLQWLHYYLQAQKGMAVTPR + Deamidated (NQ); Oxidation (M)
4030 892.4861 2674.4365 2674.3887 17.8 1 3 1 +1Score > 34 indicates identity
Score > 16 indicates homology
MTLTTSDKAAVLADALPWLTALNDK + Oxidation (M)
4956 1009.2916 5041.4216 5041.3247 19.2 1 3 1 +1Score > 30 indicates identity
Score > 16 indicates homology
GGATGGGYAQVVPMEDINLHFTGDIHAVTTAHNLLAALMDNHLHQGNE + 3 Deamidated (NQ); 2 Oxidation (M)
2806 604.9639 1811.8699 1811.8764 -3.60 1 3 0.98 +1Score > 40 indicates identity
Score > 16 indicates homology
YELPPVSVQGSSVMFR + Deamidated (NQ); Oxidation (M)
2818 607.0099 1818.0079 1817.9743 18.5 1 3 1 +1Score > 35 indicates identity
Score > 16 indicates homology
MSKSLGNVLSVPNMLTK
2969 644.3627 1930.0663 1930.0623 2.07 1 3 0.67 +1Score > 35 indicates identity
Score > 14 indicates homology
VSTDAQLSGLTVVLTGKLE
3582 762.3812 2284.1218 2284.1450 -10.2 1 3 0.83 +1Score > 39 indicates identity
Score > 15 indicates homology
EPGLTFMVAKFDGILGLGFQE + Oxidation (M)
3886 632.8302 2527.2917 2527.2451 18.5 1 3 1 +1Score > 37 indicates identity
Score > 16 indicates homology
NGLIVSCQALEGEPLHSPFIMAK + Deamidated (NQ); Oxidation (M)
4684 915.7083 3658.8041 3658.8513 -12.9 1 3 0.52 +1Score > 35 indicates identity
Score > 13 indicates homology
ADYLITSGIINDNKIMLIKPYSFMNNSGIPVAK + 3 Deamidated (NQ); Oxidation (M)
3294 711.3267 2130.9583 2130.9833 -11.7 1 3 0.99 +1Score > 38 indicates identity
Score > 16 indicates homology
NLQTLQEMYNQWPFFR + Deamidated (NQ); Oxidation (M)
3501 748.4180 2242.2322 2242.2507 -8.28 1 3 1 +1Score > 34 indicates identity
Score > 16 indicates homology
KPVLMYSIGKDSSVLLHLAR + Oxidation (M)
3976 873.0865 2616.2377 2616.2402 -0.98 0 3 0.62 +1Score > 38 indicates identity
Score > 14 indicates homology
LSVQDQPSLSPTSLQNSSSHTTTAK + 3 Deamidated (NQ)
4445 1034.5160 3100.5262 3100.5508 -7.93 1 3 0.81 +1Score > 36 indicates identity
Score > 15 indicates homology
TAVNAVGNSLPLMAMPLGVQVHNIEMHPK + Deamidated (NQ); 2 Oxidation (M)
4209 705.6302 2818.4917 2818.4535 13.6 1 3 0.56 +1Score > 34 indicates identity
Score > 13 indicates homology
IHDALVKTSTNLNSDPVDMLLHVLR + 2 Deamidated (NQ); Oxidation (M)
3862 837.1085 2508.3037 2508.3298 -10.4 1 3 1 +1Score > 37 indicates identity
Score > 16 indicates homology
VVWLEINTQPGMTPTSLVPDIAK
4398 759.6555 3034.5929 3034.6491 -18.5 1 3 0.54 +1Score > 35 indicates identity
Score > 13 indicates homology
RYYLLDAAQPLAHVQQAIDALLPTLLE
3605 768.0687 2301.1843 2301.1448 17.2 1 3 0.55 +1Score > 38 indicates identity
Score > 13 indicates homology
AVLQAPTKGASTANTTAPNASGGSK + 2 Deamidated (NQ)
3650 776.0740 2325.2002 2325.1865 5.87 1 3 1 +1Score > 38 indicates identity
Score > 16 indicates homology
NLLYIYPQSLNFANRQGSAR + Deamidated (NQ)
2503 833.4670 1664.9194 1664.8958 14.2 1 3 0.99 +1Score > 36 indicates identity
Score > 16 indicates homology
ANARVAAAILNDPDVR
3147 680.6962 2039.0668 2039.0535 6.50 1 3 0.95 +1Score > 38 indicates identity
Score > 16 indicates homology
VRGTTSPNPPVGAVIISTSGE + Deamidated (NQ)
4097 683.8713 2731.4561 2731.4254 11.2 0 3 0.6 +1Score > 34 indicates identity
Score > 14 indicates homology
MGLYANLRPAILYPQLADASSLKPE + Deamidated (NQ)
4393 1009.5422 3025.6048 3025.5551 16.4 0 3 0.69 +1Score > 34 indicates identity
Score > 14 indicates homology
LAWFGFVMPALVLNYFGQGALLLIEPE + 2 Deamidated (NQ); Oxidation (M)
4834 878.8716 4389.3216 4389.3215 0.037 1 3 1 +1Score > 29 indicates identity
Score > 16 indicates homology
LYSNLSMLLSSNYSDDNLLIINLIQQLLMYTKLQLLR + Deamidated (NQ); 2 Oxidation (M)
1550 608.3835 1214.7524 1214.7387 11.3 1 3 1 +1Score > 28 indicates identity
Score > 16 indicates homology
ATGFPIAKIAVK
2391 806.4211 1610.8276 1610.8264 0.77 0 3 0.87 +1Score > 40 indicates identity
Score > 15 indicates homology
TDPGVSAVGNAVLAPSR + Deamidated (NQ)
3167 686.0126 2055.0160 2055.0128 1.52 1 3 0.74 +1Score > 40 indicates identity
Score > 15 indicates homology
AMRSMANAVILEPLHLQE + Deamidated (NQ); 2 Oxidation (M)
3396 727.0235 2178.0487 2178.0878 -18.0 1 3 0.93 +1Score > 40 indicates identity
Score > 15 indicates homology
DLIIQYGGSVSPSNIENLMK + Deamidated (NQ)
3065 664.3481 1990.0225 1990.0372 -7.38 0 3 0.73 +1Score > 40 indicates identity
Score > 14 indicates homology
IVSFSGSPVGSGTSISGGGPIK
3684 786.4159 2356.2259 2356.1984 11.6 1 3 0.75 +1Score > 37 indicates identity
Score > 15 indicates homology
QPAEVIPQQMIGFSDPAVSTLK + Deamidated (NQ)
2639 862.9893 1723.9640 1723.9369 15.7 1 3 0.51 +1Score > 36 indicates identity
Score > 13 indicates homology
VLISYYLRNSNNLR
2482 550.9849 1649.9329 1649.9464 -8.22 1 3 0.75 +1Score > 34 indicates identity
Score > 15 indicates homology
IDPNTPVKLLSIGQR
2507 556.3100 1665.9082 1665.8838 14.6 1 3 0.79 +1Score > 37 indicates identity
Score > 15 indicates homology
IGVHARPITAGVFEAE
4037 895.4534 2683.3384 2683.3202 6.79 1 3 0.57 +1Score > 37 indicates identity
Score > 13 indicates homology
VTNSSDDWTISGIPANQHRLTLTR + 2 Deamidated (NQ)
2860 927.9538 1853.8930 1853.8867 3.41 0 3 0.94 +1Score > 40 indicates identity
Score > 16 indicates homology
QAAVQAAVNSNHNSLATR + 3 Deamidated (NQ)
3131 1018.4969 2034.9792 2034.9424 18.1 1 3 0.63 +1Score > 40 indicates identity
Score > 14 indicates homology
AMINAMQPGDVIMLENVR + 2 Deamidated (NQ); 2 Oxidation (M)
4604 857.2204 3424.8525 3424.8615 -2.62 0 3 0.73 +1Score > 30 indicates identity
Score > 14 indicates homology
TQAGWLLIASLMTLGPAVVQSQLGCLLLLWR + Deamidated (NQ); Oxidation (M)
2504 833.4684 1664.9222 1664.9209 0.78 1 3 0.84 +1Score > 36 indicates identity
Score > 15 indicates homology
GAGAPVLELAGVAATAAAR
3451 735.4116 2203.2130 2203.1736 17.9 1 3 0.72 +1Score > 34 indicates identity
Score > 14 indicates homology
SLNFITLPLDKIQNQTLSR + 3 Deamidated (NQ)
603 790.4311 789.4238 789.4167 9.02 0 3 0.81 +1Score > 41 indicates identity
Score > 15 indicates homology
MAAAVGVR + Oxidation (M)
3007 651.3666 1951.0780 1951.0924 -7.41 1 3 0.51 +1Score > 35 indicates identity
Score > 13 indicates homology
VLLDPARAGAAGVMQQIIK + Deamidated (NQ)
3334 716.7234 2147.1484 2147.1408 3.52 1 3 0.95 +1Score > 36 indicates identity
Score > 16 indicates homology
QYQQQIVNNAKALAVALMK + Deamidated (NQ); Oxidation (M)
3400 727.0831 2178.2275 2178.2160 5.26 1 3 0.62 +1Score > 32 indicates identity
Score > 14 indicates homology
LAQVALNRYPAPRPAALLDK + 2 Deamidated (NQ)
725 848.4510 847.4437 847.4586 -17.5 1 3 1 +1Score > 42 indicates identity
Score > 16 indicates homology
MTPSLKR + Oxidation (M)
2799 604.3224 1809.9454 1809.9261 10.6 0 3 0.63 +1Score > 39 indicates identity
Score > 14 indicates homology
QLFGFVTVSIVSAGGGDR + Deamidated (NQ)
2522 835.4322 1668.8498 1668.8795 -17.7 1 3 0.8 +1Score > 40 indicates identity
Score > 15 indicates homology
LNRILNDQLDAVQR + 2 Deamidated (NQ)
665 411.7322 821.4498 821.4396 12.5 1 3 0.84 +1Score > 39 indicates identity
Score > 15 indicates homology
DTVKGFR
3578 761.3995 2281.1767 2281.1644 5.39 1 3 0.92 +1Score > 38 indicates identity
Score > 15 indicates homology
KAWAFGTNAHLPDDIAVAWAK
152 497.2848 496.2775 496.2758 3.53 0 3 1 +1Score > 34 indicates identity
Score > 16 indicates homology
AGPPR
2947 639.0052 1913.9938 1913.9694 12.7 1 3 0.6 +1Score > 39 indicates identity
Score > 13 indicates homology
GVTTGTPIALEIQNTDQR + Deamidated (NQ)
3123 1016.5172 2031.0198 2031.0021 8.74 1 3 0.74 +1Score > 39 indicates identity
Score > 14 indicates homology
QEILSSNIANADTPGFQAR
3219 697.7368 2090.1886 2090.1520 17.5 1 3 1 +1Score > 31 indicates identity
Score > 16 indicates homology
EQFLLSVGLLVCLVCLVK + Deamidated (NQ)
3339 717.0574 2148.1504 2148.1327 8.21 1 3 0.66 +1Score > 36 indicates identity
Score > 14 indicates homology
RLLSQGLPGLDGPPFPAQQR + 2 Deamidated (NQ)
3320 1070.5477 2139.0808 2139.0973 -7.71 1 3 0.78 +1Score > 39 indicates identity
Score > 15 indicates homology
ARPIWYVGRDPAAAPATGNR + Deamidated (NQ)
3352 719.0743 2154.2011 2154.1644 17.0 1 3 0.99 +1Score > 33 indicates identity
Score > 16 indicates homology
RGNQVIIAGDGQVSLGQTVIK + 2 Deamidated (NQ)
3745 800.7640 2399.2702 2399.2316 16.1 0 3 0.78 +1Score > 36 indicates identity
Score > 15 indicates homology
LPPVHMFGGINLSFGLMGPLMR + Oxidation (M)
4502 1063.8882 3188.6428 3188.6131 9.31 1 3 0.6 +1Score > 35 indicates identity
Score > 13 indicates homology
IVCLGSKGLMACQSIGLNVIASAVNLGDTPK + 3 Deamidated (NQ)
4580 1116.2395 3345.6967 3345.6434 15.9 1 3 0.6 +1Score > 35 indicates identity
Score > 13 indicates homology
IAVSQLIDLAMLPLDMFSMFSGIESTVNAAK + 2 Deamidated (NQ); 2 Oxidation (M)
3807 616.3383 2461.3241 2461.3176 2.63 1 3 0.55 +1Score > 35 indicates identity
Score > 13 indicates homology
ADPSLNNPVVIQATRLDASILPR + 2 Deamidated (NQ)
3459 737.0623 2208.1651 2208.1473 8.04 1 3 1 +1Score > 37 indicates identity
Score > 16 indicates homology
NLTYAKNNCIILHPGPINR + Deamidated (NQ)
3764 806.4564 2416.3474 2416.3036 18.1 1 3 0.56 +1Score > 32 indicates identity
Score > 13 indicates homology
VKNICIVLSGGNVDLTSLTWVK + Deamidated (NQ)
3849 624.5493 2494.1681 2494.1711 -1.19 1 3 0.72 +1Score > 38 indicates identity
Score > 14 indicates homology
TDSIYNQLTQAGLNVLLDDRNE + 3 Deamidated (NQ)
4503 799.4294 3193.6885 3193.6594 9.11 1 3 1 +1Score > 33 indicates identity
Score > 16 indicates homology
NTRMALAVVDTFVWQSLASVAIPGFTINR + Deamidated (NQ); Oxidation (M)
4003 662.5946 2646.3493 2646.3192 11.4 0 3 0.84 +1Score > 37 indicates identity
Score > 15 indicates homology
WWQVFYTVVDYINQIVQVMSK + Deamidated (NQ)
3523 751.3479 2251.0219 2251.0334 -5.14 1 3 0.59 +1Score > 38 indicates identity
Score > 13 indicates homology
NAGDFTAVDYSGKNFWFGVR + Deamidated (NQ)
3895 848.7717 2543.2933 2543.2770 6.38 0 3 0.87 +1Score > 38 indicates identity
Score > 15 indicates homology
FFGIYTATAVAMVASLTQVAFYR + Deamidated (NQ); Oxidation (M)
3814 822.4360 2464.2862 2464.2394 19.0 1 3 0.83 +1Score > 37 indicates identity
Score > 15 indicates homology
AVVIGHNHGFTLRGGNMTSPDIR + Oxidation (M)
3931 857.1519 2568.4339 2568.3985 13.8 1 3 1 +1Score > 30 indicates identity
Score > 16 indicates homology
AAAQGLMAGINAVLALKNQDPFILK + 2 Deamidated (NQ)
3977 873.7428 2618.2066 2618.2356 -11.1 1 3 0.68 +1Score > 37 indicates identity
Score > 14 indicates homology
IIVTNQTTMSQWDVQALMEHVR + 3 Deamidated (NQ); Oxidation (M)
4801 1047.5063 4185.9961 4185.9349 14.6 1 3 1 +1Score > 32 indicates identity
Score > 16 indicates homology
QPQSQSQSQQLQPQPQPQPQLQPQLQSQPQSQQLKR + 12 Deamidated (NQ)
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