MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1740 434.9125 1301.7157 1301.7377 -16.9 1 4 0.61 +1Score > 40 indicates identity
Score > 15 indicates homology
KIIINMGLGQAK + Deamidated (NQ); Oxidation (M)
3855 834.4536 2500.3390 2500.3094 11.8 1 4 1 +1Score > 35 indicates identity
Score > 17 indicates homology
TSVVLQLDNVPGALVSAMTELAVR + 2 Deamidated (NQ); Oxidation (M)
2530 557.6375 1669.8907 1669.8749 9.44 0 4 0.41 +1Score > 39 indicates identity
Score > 13 indicates homology
MLITQGYAVALFGSAK + Deamidated (NQ)
4399 1013.5150 3037.5232 3037.5066 5.45 0 4 0.67 +1Score > 36 indicates identity
Score > 15 indicates homology
GTAIYDLPATNGVPIVADMSSNILAAQYR + Deamidated (NQ); Oxidation (M)
255 312.1871 622.3596 622.3551 7.33 0 4 0.56 +1Score > 33 indicates identity
Score > 14 indicates homology
TPIHR
2935 636.6600 1906.9582 1906.9360 11.6 0 4 0.8 +1Score > 40 indicates identity
Score > 16 indicates homology
VAIDIEPSVHAGMPYHR + Oxidation (M)
200 586.3876 585.3803 585.3737 11.2 0 4 0.48 +1Score > 39 indicates identity
Score > 14 indicates homology
ILDIL
2213 511.2888 1530.8446 1530.8592 -9.56 1 4 0.97 +1Score > 38 indicates identity
Score > 17 indicates homology
FSNQKVPMIAVIGK
2820 911.9158 1821.8170 1821.7985 10.2 1 4 0.65 +1Score > 38 indicates identity
Score > 15 indicates homology
NAKLDMNGNCLNAVDR + 2 Deamidated (NQ); Oxidation (M)
3494 1119.5756 2237.1366 2237.1362 0.21 0 4 0.68 +1Score > 39 indicates identity
Score > 15 indicates homology
TAQAIAVSLQSLMGVFTQAQR + 2 Deamidated (NQ); Oxidation (M)
4417 1021.8837 3062.6293 3062.6037 8.36 0 4 0.95 +1Score > 33 indicates identity
Score > 17 indicates homology
HYGVGGDGLLTAVHLATLVQDSGLSLAQLR + 2 Deamidated (NQ)
3790 611.2933 2441.1441 2441.1209 9.52 1 4 0.41 +1Score > 38 indicates identity
Score > 13 indicates homology
YSPDAWMLNYSNPAAIVAEATR + 2 Deamidated (NQ)
4360 993.8671 2978.5795 2978.6116 -10.8 1 4 0.45 +1Score > 34 indicates identity
Score > 13 indicates homology
NYSSNTQTIIKPYVIDLINNKLIWK + Deamidated (NQ)
3168 686.7200 2057.1382 2057.1330 2.53 1 4 1 +1Score > 34 indicates identity
Score > 17 indicates homology
IEIPLTLSIIQSMLVATSE
4015 887.4412 2659.3018 2659.3011 0.26 1 4 0.49 +1Score > 38 indicates identity
Score > 14 indicates homology
MGNGLSLDEAGNLTSQNVTTVSPPLK + Deamidated (NQ); Oxidation (M)
4322 586.3043 2926.4851 2926.4344 17.3 1 4 0.83 +1Score > 37 indicates identity
Score > 16 indicates homology
QKITNIVFMGMGEPLLNTLNVFDAVE + 2 Deamidated (NQ); 2 Oxidation (M)
4428 771.3948 3081.5501 3081.5474 0.86 1 4 0.76 +1Score > 36 indicates identity
Score > 16 indicates homology
VNLNGGAIALGHPLGCSGARITTTLLNLME + 3 Deamidated (NQ); Oxidation (M)
3793 816.0829 2445.2269 2445.2420 -6.21 0 4 0.65 +1Score > 38 indicates identity
Score > 15 indicates homology
LTLMIAGNIATNVLNQQVAASQR + 4 Deamidated (NQ); Oxidation (M)
2227 769.8933 1537.7720 1537.8028 -20.0 1 4 0.58 +1Score > 40 indicates identity
Score > 14 indicates homology
APELIFGAINYTTK + Deamidated (NQ)
2048 728.8807 1455.7468 1455.7583 -7.84 1 4 0.51 +1Score > 40 indicates identity
Score > 14 indicates homology
GKNVFVGAHGNSIR + Deamidated (NQ)
2593 853.9846 1705.9546 1705.9363 10.8 1 4 0.78 +1Score > 35 indicates identity
Score > 16 indicates homology
SPIVEPGLEALLQQGR
3413 729.3465 2185.0177 2184.9786 17.9 1 4 0.94 +1Score > 39 indicates identity
Score > 16 indicates homology
YYAMLSKTGVHHYSGNNIE + 2 Deamidated (NQ)
2573 567.3189 1698.9349 1698.9264 4.97 1 4 0.84 +1Score > 37 indicates identity
Score > 16 indicates homology
KPDVLGQKTATNAISR + Deamidated (NQ)
4044 897.4612 2689.3618 2689.4010 -14.6 0 4 0.83 +1Score > 37 indicates identity
Score > 16 indicates homology
HVHTLVMAAGYAVANLDATVIAQAPR + Deamidated (NQ)
2518 834.4698 1666.9250 1666.9253 -0.17 1 4 0.74 +1Score > 36 indicates identity
Score > 15 indicates homology
KPLIATTNEISINPR + Deamidated (NQ)
3146 680.3687 2038.0843 2038.0833 0.46 1 4 0.68 +1Score > 37 indicates identity
Score > 15 indicates homology
VNIATLPLQVNINVQRLE + 5 Deamidated (NQ)
4991 1165.6248 5823.0876 5823.0366 8.76 1 4 1 +1Score > 25 indicates identity
Score > 17 indicates homology
SPVVDSAVATLSSPSIAVFGGVSFKTNGGLPSGMPLTSILNSLNHCLLVGSAIIQVLE + 2 Deamidated (NQ)
3796 816.4345 2446.2817 2446.2334 19.7 0 4 0.99 +1Score > 37 indicates identity
Score > 17 indicates homology
QILVQSGTAMLAQANMKPNSVLK + 5 Deamidated (NQ)
4120 922.1459 2763.4159 2763.4218 -2.15 1 4 0.69 +1Score > 37 indicates identity
Score > 15 indicates homology
NSEPTDLIIWSEAALIVPDDIPQVK + Deamidated (NQ)
2519 834.9249 1667.8352 1667.8660 -18.4 1 4 0.58 +1Score > 40 indicates identity
Score > 14 indicates homology
AKMMAALTMLLDALK + 3 Oxidation (M)
3955 863.7831 2588.3275 2588.3494 -8.48 1 4 0.92 +1Score > 37 indicates identity
Score > 16 indicates homology
ALVLPAYDMVLKASHLFNLMNAR + 2 Deamidated (NQ)
4804 600.7318 4198.0717 4198.0802 -2.04 1 4 0.43 +1Score > 33 indicates identity
Score > 13 indicates homology
DFTPLLPGCVSIYLCGATVQAAPHIGHIRSGLNFDIMR + 2 Deamidated (NQ)
3663 780.0694 2337.1864 2337.2216 -15.1 1 4 0.72 +1Score > 38 indicates identity
Score > 15 indicates homology
VYPLASYVTVNISSPNTKNLR + 2 Deamidated (NQ)
3201 692.3843 2074.1311 2074.1324 -0.62 1 4 1 +1Score > 35 indicates identity
Score > 17 indicates homology
QITPKPIGVGFGIASPEHAR
3934 858.1462 2571.4168 2571.3988 6.97 1 4 0.8 +1Score > 32 indicates identity
Score > 16 indicates homology
LWYGNAGLLVTEVFLPPLLQSLE
4870 890.4750 4447.3386 4447.3501 -2.58 1 4 1 +1Score > 29 indicates identity
Score > 17 indicates homology
VAINSIATMNLPLFDTRLNNIGSLTLDYQIIFPYPGNPLK + Deamidated (NQ)
3973 872.4565 2614.3477 2614.3829 -13.5 1 4 0.45 +1Score > 37 indicates identity
Score > 13 indicates homology
MVELNVYNGIPHLLIPVVTNPHK + 2 Deamidated (NQ); Oxidation (M)
4716 762.4029 3806.9781 3806.9691 2.37 1 4 1 +1Score > 32 indicates identity
Score > 17 indicates homology
GVLTFPKSDNVNVVVDGDILTLSANDPSHVSLAGTVR + Deamidated (NQ)
3164 685.0483 2052.1231 2052.1215 0.77 0 4 0.96 +1Score > 35 indicates identity
Score > 16 indicates homology
IDLTANGTLPGIIQANATLR + Deamidated (NQ)
1364 378.8714 1133.5924 1133.6054 -11.5 0 4 0.96 +1Score > 42 indicates identity
Score > 16 indicates homology
AHSSLGPGRPR
3368 1083.5310 2165.0474 2165.0510 -1.65 0 4 0.94 +1Score > 39 indicates identity
Score > 16 indicates homology
QIRPDFVMAVMSGNWVQR + 2 Oxidation (M)
3657 777.7544 2330.2414 2330.2093 13.8 1 4 0.65 +1Score > 36 indicates identity
Score > 15 indicates homology
IGFNAKFQNVIYASMITALGR + Deamidated (NQ); Oxidation (M)
3767 806.7602 2417.2588 2417.2672 -3.47 1 4 0.59 +1Score > 37 indicates identity
Score > 14 indicates homology
SLHPHVPGVTNLQVMRAMASLK + 2 Oxidation (M)
4748 797.8221 3984.0741 3984.0096 16.2 1 4 0.46 +1Score > 32 indicates identity
Score > 13 indicates homology
MPPGIGNTFLIVMMGEFIIAMLGNGFIVLVNCIDVR + Deamidated (NQ); 2 Oxidation (M)
658 816.4153 815.4080 815.4137 -6.99 0 4 0.51 +1Score > 41 indicates identity
Score > 14 indicates homology
DDGQLLR
3694 590.5585 2358.2049 2358.1676 15.8 1 4 0.77 +1Score > 38 indicates identity
Score > 15 indicates homology
AGAATPRTAPGHSTPAVTGANQPAR + 2 Deamidated (NQ)
3718 794.0501 2379.1285 2379.0900 16.2 1 4 0.88 +1Score > 39 indicates identity
Score > 16 indicates homology
IATTRPETMFGDTAIAVNPNDE + Deamidated (NQ); Oxidation (M)
3226 699.3721 2095.0945 2095.0660 13.6 1 4 1 +1Score > 38 indicates identity
Score > 16 indicates homology
VQDSLFKPSSATWKLIME + Oxidation (M)
3606 1151.5996 2301.1846 2301.1470 16.3 0 4 0.83 +1Score > 38 indicates identity
Score > 16 indicates homology
QSVLNLMTRPSVHQGQGMYR
3875 840.4285 2518.2637 2518.2236 15.9 1 4 0.56 +1Score > 38 indicates identity
Score > 14 indicates homology
LQYYLSLMNQQLPIESQFMR + Deamidated (NQ); Oxidation (M)
4252 955.2134 2862.6184 2862.5677 17.7 1 4 1 +1Score > 28 indicates identity
Score > 16 indicates homology
LPLDSQYLDVLGLPIHMVKIQVAAGR + Deamidated (NQ); Oxidation (M)
3449 735.3558 2203.0456 2203.0566 -5.00 1 4 0.77 +1Score > 39 indicates identity
Score > 15 indicates homology
EGVSGQVLVDSNDLLTMPLSE + Deamidated (NQ)
4743 989.5385 3954.1249 3954.1142 2.69 1 4 0.7 +1Score > 29 indicates identity
Score > 15 indicates homology
NLVAALGGSGLIVLFPNSPSGLLGAHYTKTPQGSLIFDK + 2 Deamidated (NQ)
2316 788.4145 1574.8144 1574.7908 15.0 1 4 0.71 +1Score > 40 indicates identity
Score > 15 indicates homology
GLKNVAAGANPMIMR + Deamidated (NQ); 2 Oxidation (M)
2317 525.9590 1574.8552 1574.8239 19.9 1 4 0.92 +1Score > 38 indicates identity
Score > 16 indicates homology
MGLHTKISNIFAAR + Deamidated (NQ); Oxidation (M)
2553 561.9845 1682.9317 1682.8991 19.3 1 4 0.69 +1Score > 37 indicates identity
Score > 15 indicates homology
YAARVNSLDYLAVTK
2965 483.0180 1928.0429 1928.0116 16.3 1 4 0.6 +1Score > 37 indicates identity
Score > 14 indicates homology
TQPPAPPGVSPQALPSRAR + 2 Deamidated (NQ)
4995 999.0188 5988.0691 5987.9886 13.4 1 4 1 +1Score > 28 indicates identity
Score > 16 indicates homology
ATMMAIVQTIRDVVQFNQLFGQQINLLLHPSQNVIDNPVYLCDLVATLVQSAE + 5 Deamidated (NQ); Oxidation (M)
4412 1021.5253 3061.5541 3061.6079 -17.6 1 4 0.9 +1Score > 36 indicates identity
Score > 16 indicates homology
IVKVLFNMGVMATINQSLDQDTAVLIVE + Deamidated (NQ)
4565 829.4272 3313.6797 3313.6136 19.9 1 4 1 +1Score > 35 indicates identity
Score > 16 indicates homology
NTVTQMVYKHAISTVVPARPVNIQQQDSGE + 4 Deamidated (NQ)
4766 1012.7324 4046.9005 4046.8792 5.27 1 4 0.53 +1Score > 33 indicates identity
Score > 14 indicates homology
LQHQWDAISGMPTNLYGQNDNFHPENSHVLPALMR + Deamidated (NQ); Oxidation (M)
3843 830.7985 2489.3737 2489.3781 -1.78 1 4 0.99 +1Score > 32 indicates identity
Score > 16 indicates homology
LILASVLQGFFDAVALLLRSNVE + 2 Deamidated (NQ)
4276 963.1356 2886.3850 2886.3521 11.4 1 4 0.74 +1Score > 37 indicates identity
Score > 15 indicates homology
LNTSAIEQVANFLAYFMILHDVQTE + 4 Deamidated (NQ); Oxidation (M)
2071 489.6344 1465.8814 1465.8617 13.4 1 4 1 +1Score > 30 indicates identity
Score > 16 indicates homology
IIVVVGAPGDKGVSR
3232 1050.5425 2099.0704 2099.0932 -10.8 1 4 1 +1Score > 39 indicates identity
Score > 16 indicates homology
MNLTKIQNLTIHNITGIR + 4 Deamidated (NQ); Oxidation (M)
2718 882.4892 1762.9638 1762.9287 19.9 1 4 0.58 +1Score > 37 indicates identity
Score > 14 indicates homology
YMGPAGAKNLQSVVLSK + Deamidated (NQ)
4165 930.8046 2789.3920 2789.3468 16.2 1 4 0.66 +1Score > 37 indicates identity
Score > 15 indicates homology
RNNAFGGADLVISGNVPQGAGLSSSASLE + 2 Deamidated (NQ)
651 407.2298 812.4450 812.4392 7.21 1 4 0.99 +1Score > 38 indicates identity
Score > 16 indicates homology
AGPEQALK
3689 1180.1052 2358.1958 2358.2154 -8.31 1 4 0.78 +1Score > 38 indicates identity
Score > 15 indicates homology
APYVDIVLGPQTYHRLGGMVR + Deamidated (NQ); Oxidation (M)
3839 828.1102 2481.3088 2481.2785 12.2 0 4 0.84 +1Score > 36 indicates identity
Score > 16 indicates homology
SIPVGTDLPQTIDNGLSQLIPMR + Deamidated (NQ); Oxidation (M)
3374 1085.5936 2169.1726 2169.1793 -3.07 1 4 0.51 +1Score > 36 indicates identity
Score > 13 indicates homology
LQNLKLNVLNLSYNHLSGK + 2 Deamidated (NQ)
3444 734.0667 2199.1783 2199.1762 0.95 0 4 0.74 +1Score > 36 indicates identity
Score > 15 indicates homology
YPVVPIMINAVAPPLASFQR + Deamidated (NQ); Oxidation (M)
3871 838.4338 2512.2796 2512.2420 14.9 1 4 0.76 +1Score > 38 indicates identity
Score > 15 indicates homology
FEIVLYGATGFSGMLTGQHLAQR + Deamidated (NQ); Oxidation (M)
4019 666.8436 2663.3453 2663.3472 -0.70 1 4 0.76 +1Score > 37 indicates identity
Score > 15 indicates homology
IASLDEIDMVVTSVVGMIGLEPTMK + Oxidation (M)
4069 903.4931 2707.4575 2707.4466 4.03 1 4 1 +1Score > 34 indicates identity
Score > 16 indicates homology
QMGAIITNSLSLKTNYLIVGINAGSK + 2 Deamidated (NQ)
4230 710.3771 2837.4793 2837.4256 18.9 0 4 0.79 +1Score > 35 indicates identity
Score > 15 indicates homology
NLNFLTDTLSQLSQTLAAGDVILMGAK + 4 Deamidated (NQ)
3644 581.8121 2323.2193 2323.2206 -0.54 1 4 0.54 +1Score > 37 indicates identity
Score > 14 indicates homology
ADAKGGLDGLLAQSLSGLNAMPPK
3799 818.4568 2452.3486 2452.3584 -4.01 1 4 0.64 +1Score > 33 indicates identity
Score > 14 indicates homology
NSMVISTPAGQIGVVQIAGLVARR + Oxidation (M)
3389 725.3679 2173.0819 2173.0750 3.16 1 4 0.97 +1Score > 39 indicates identity
Score > 16 indicates homology
EPSSAQLLLTQSEGIDLTDR + Deamidated (NQ)
3633 772.7169 2315.1289 2315.1216 3.15 1 4 0.73 +1Score > 39 indicates identity
Score > 15 indicates homology
MAQSTTYSRVLGTGSYLPPNR + Deamidated (NQ); Oxidation (M)
3639 774.3657 2320.0753 2320.0971 -9.42 1 4 0.71 +1Score > 38 indicates identity
Score > 15 indicates homology
NLLDTQWRDSLLNPSFDQR + 3 Deamidated (NQ)
4311 971.5081 2911.5025 2911.5080 -1.89 1 4 0.47 +1Score > 36 indicates identity
Score > 13 indicates homology
ILDGGNPFLDVPQALNATTYKHGVLTR + 2 Deamidated (NQ)
4749 998.4888 3989.9261 3989.8576 17.2 1 4 0.95 +1Score > 34 indicates identity
Score > 16 indicates homology
TGDTNILTIAFVSTDGSMDKQDIADYVASNIQDPLSR + 3 Deamidated (NQ); Oxidation (M)
310 652.3691 651.3618 651.3704 -13.2 1 4 0.53 +1Score > 36 indicates identity
Score > 13 indicates homology
TPVAKH
3292 710.4166 2128.2280 2128.2183 4.53 1 4 1 +1Score > 29 indicates identity
Score > 16 indicates homology
ASGAYPALLLVLLAFALPESL
3815 822.7813 2465.3221 2465.3125 3.86 1 4 0.83 +1Score > 35 indicates identity
Score > 15 indicates homology
IESAVADGPQSVILNQVTNGIAIR + Deamidated (NQ)
4609 1144.6001 3430.7785 3430.7377 11.9 1 4 1 +1Score > 34 indicates identity
Score > 16 indicates homology
LIVILNDNDMSIAPPVGAMSHYFARLVSSQR + Deamidated (NQ); Oxidation (M)
3136 1019.5200 2037.0254 2037.0201 2.65 1 4 0.71 +1Score > 39 indicates identity
Score > 15 indicates homology
SLGSANPYNMIRATIDGLK + Deamidated (NQ); Oxidation (M)
3302 712.3611 2134.0615 2134.0556 2.76 1 4 0.79 +1Score > 39 indicates identity
Score > 15 indicates homology
GVVRFQGGHNAGHTLVIGANE + 2 Deamidated (NQ)
3095 1006.5262 2011.0378 2011.0738 -17.9 1 4 0.89 +1Score > 39 indicates identity
Score > 16 indicates homology
LGITQNIVAIVNEYAHGAK + Deamidated (NQ)
3592 766.7657 2297.2753 2297.2420 14.5 1 4 1 +1Score > 33 indicates identity
Score > 16 indicates homology
LTARLAGYSGSIQFIQVPFTK + Deamidated (NQ)
3703 787.7577 2360.2513 2360.2124 16.4 1 4 0.99 +1Score > 36 indicates identity
Score > 16 indicates homology
THQISAAPTSSAGDGFKANLIFK
3989 878.4548 2632.3426 2632.3067 13.6 1 4 0.72 +1Score > 37 indicates identity
Score > 15 indicates homology
SGMNNIFRVGGAHDSLSPLQYLVR + 2 Deamidated (NQ)
3846 832.3939 2494.1599 2494.1984 -15.5 1 4 0.87 +1Score > 38 indicates identity
Score > 15 indicates homology
ILDSSCPQTMHLKFAAAYNLGR + 2 Deamidated (NQ)
4104 917.1326 2748.3760 2748.3251 18.5 1 4 0.77 +1Score > 38 indicates identity
Score > 15 indicates homology
DYPDVVLSHMYVDNAAMQLVRAPK + Deamidated (NQ); Oxidation (M)
865 906.4749 905.4676 905.4719 -4.73 1 4 0.79 +1Score > 41 indicates identity
Score > 15 indicates homology
HLVHEGSK
3229 699.6924 2096.0554 2096.0195 17.1 0 4 0.56 +1Score > 39 indicates identity
Score > 14 indicates homology
VSAITLVSATSTTANMTVSPE + Deamidated (NQ); Oxidation (M)
4161 929.5134 2785.5184 2785.5059 4.49 0 4 1 +1Score > 32 indicates identity
Score > 16 indicates homology
MLPVLFVTFVPSMMLAQIMTLFIK + Oxidation (M)
3768 806.7606 2417.2600 2417.2836 -9.76 1 4 0.63 +1Score > 37 indicates identity
Score > 14 indicates homology
QTLITNLQALDCATSLGTKLQK + Deamidated (NQ)
4233 947.4940 2839.4602 2839.4493 3.83 1 4 0.52 +1Score > 36 indicates identity
Score > 13 indicates homology
TVFHHLARYLFHPTNQVWGMITR + Oxidation (M)
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