MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3556 1136.0703 2270.1260 2270.1028 10.3 1 5 0.83 +1Score > 39 indicates identity
Score > 17 indicates homology
STIAQYLTQKYAPSLNMPLE + 3 Deamidated (NQ)
1142 1016.5383 1015.5310 1015.5410 -9.87 1 5 0.66 +1Score > 42 indicates identity
Score > 16 indicates homology
GRVGQLTQR + 2 Deamidated (NQ)
2926 633.6575 1897.9507 1897.9269 12.5 0 5 0.37 +1Score > 39 indicates identity
Score > 13 indicates homology
LDDVNDVDQQALLPSVR + 2 Deamidated (NQ)
3596 1150.6130 2299.2114 2299.2212 -4.26 1 5 0.5 +1Score > 37 indicates identity
Score > 14 indicates homology
LKFFSIGTNDLTQYVLAVDR
3823 824.4381 2470.2925 2470.3319 -16.0 1 5 0.97 +1Score > 37 indicates identity
Score > 17 indicates homology
NITVLEPVTQPFLQVTNTTVKE
1460 586.3005 1170.5864 1170.6067 -17.3 0 5 0.68 +1Score > 40 indicates identity
Score > 16 indicates homology
VPDALDILCR
3730 795.7641 2384.2705 2384.3176 -19.8 1 5 0.7 +1Score > 36 indicates identity
Score > 16 indicates homology
IQVQNFRAGLSNILVVTDVAAR + Deamidated (NQ)
2357 795.4269 1588.8392 1588.8243 9.43 1 5 0.93 +1Score > 40 indicates identity
Score > 17 indicates homology
LATRGVDAATLNQMK + Deamidated (NQ)
4049 898.4613 2692.3621 2692.3391 8.53 1 5 0.64 +1Score > 37 indicates identity
Score > 15 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
1795 442.8989 1325.6749 1325.6840 -6.91 0 5 0.89 +1Score > 41 indicates identity
Score > 17 indicates homology
SGLQHVATFNPR
3268 707.0518 2118.1336 2118.1395 -2.78 1 5 0.89 +1Score > 37 indicates identity
Score > 17 indicates homology
MDLFLVQAVLQQLSLKDR + 2 Deamidated (NQ)
3842 830.7659 2489.2759 2489.2480 11.2 1 5 0.79 +1Score > 37 indicates identity
Score > 16 indicates homology
LPGNGWAMQLLAVTCLSLAAKME + Oxidation (M)
2974 644.6995 1931.0767 1931.0476 15.0 1 5 1 +1Score > 34 indicates identity
Score > 17 indicates homology
VLLSVTGEQYGRPISGQK
3215 1044.0522 2086.0898 2086.0782 5.57 1 5 0.58 +1Score > 38 indicates identity
Score > 15 indicates homology
VFRAPYVGAVVMPGGADQPR
4273 722.3857 2885.5137 2885.5298 -5.58 0 5 0.41 +1Score > 35 indicates identity
Score > 13 indicates homology
IIYLMFFMVITMAFIGSVIFGVTTR + Oxidation (M)
4455 779.6852 3114.7117 3114.6563 17.8 1 5 0.52 +1Score > 30 indicates identity
Score > 15 indicates homology
VVIKPLGAMAGESIFYLTVNDPNIPVVIE + Deamidated (NQ); Oxidation (M)
4600 849.9249 3395.6705 3395.6721 -0.46 1 5 0.87 +1Score > 36 indicates identity
Score > 17 indicates homology
YDVSGIQIHDMGLAKYINLNSYFNLHTGGR
1999 477.2704 1428.7894 1428.7712 12.7 1 5 0.7 +1Score > 39 indicates identity
Score > 16 indicates homology
LNITVELLAVTDE
2789 452.7334 1806.9045 1806.9363 -17.6 1 5 0.82 +1Score > 40 indicates identity
Score > 16 indicates homology
IIAGLEAADSGQILLHGE + Deamidated (NQ)
3100 672.7099 2015.1079 2015.0938 6.96 1 5 0.7 +1Score > 35 indicates identity
Score > 16 indicates homology
IINEPTAAALAYGLDKNLK + Deamidated (NQ)
4172 932.4829 2794.4269 2794.3994 9.85 0 5 0.7 +1Score > 36 indicates identity
Score > 16 indicates homology
GDVVLPGDHNVDNVLAAMSAALLMGATK + Oxidation (M)
2962 963.4904 1924.9662 1924.9451 11.0 1 5 1 +1Score > 39 indicates identity
Score > 17 indicates homology
LHNISLALCDVALENLE + 2 Deamidated (NQ)
3161 684.3807 2050.1203 2050.0834 18.0 0 5 0.79 +1Score > 35 indicates identity
Score > 16 indicates homology
IINTNSTITFNQIVQLTK + 3 Deamidated (NQ)
3816 823.0609 2466.1609 2466.1333 11.2 1 5 0.47 +1Score > 38 indicates identity
Score > 14 indicates homology
VDASMVAVNASSRFNDGGQLGLGAE + 2 Deamidated (NQ)
872 908.4785 907.4712 907.4619 10.2 1 5 0.71 +1Score > 41 indicates identity
Score > 16 indicates homology
KVMMDLR + Oxidation (M)
3654 777.4374 2329.2904 2329.2563 14.6 0 5 0.93 +1Score > 33 indicates identity
Score > 17 indicates homology
TIGDLSQIQSKPVLIVSMDIR + Deamidated (NQ); Oxidation (M)
3773 606.5455 2422.1529 2422.1620 -3.77 1 5 1 +1Score > 39 indicates identity
Score > 17 indicates homology
TQNMAWSKPRNVQILNMVTR + 4 Deamidated (NQ); 2 Oxidation (M)
3878 841.7974 2522.3704 2522.3268 17.3 1 5 0.52 +1Score > 34 indicates identity
Score > 14 indicates homology
FLGVDEQVQLADLLPRPALAQVE + 2 Deamidated (NQ)
4101 913.8388 2738.4946 2738.4491 16.6 1 5 1 +1Score > 33 indicates identity
Score > 17 indicates homology
VVDAILAKGGYAIITADHGNADVLITE
2459 545.6317 1633.8733 1633.8716 1.04 0 5 0.67 +1Score > 39 indicates identity
Score > 15 indicates homology
FILPNVSTPVSDAFK
4308 970.5363 2908.5871 2908.5741 4.46 1 5 0.85 +1Score > 32 indicates identity
Score > 16 indicates homology
GPIGIGTLLTVCLGGMLLHVFMPIVER + Oxidation (M)
666 823.4019 822.3946 822.3905 4.96 0 5 0.67 +1Score > 41 indicates identity
Score > 15 indicates homology
VSQMVSR + Deamidated (NQ); Oxidation (M)
3275 1061.0759 2120.1372 2120.1663 -13.7 1 5 0.87 +1Score > 36 indicates identity
Score > 17 indicates homology
LLSKILMAQNVPHNVLNAK + 2 Deamidated (NQ); Oxidation (M)
4048 898.4581 2692.3525 2692.3314 7.82 1 5 0.92 +1Score > 37 indicates identity
Score > 17 indicates homology
NKFAVWLMTIIVTAAGLYAGMNMK + 2 Deamidated (NQ); 3 Oxidation (M)
4051 898.7852 2693.3338 2693.3332 0.23 0 5 0.7 +1Score > 38 indicates identity
Score > 16 indicates homology
NNNITLATLMAITALLNLFFYMR + 4 Deamidated (NQ); 2 Oxidation (M)
4377 1004.5199 3010.5379 3010.5045 11.1 1 5 0.5 +1Score > 36 indicates identity
Score > 14 indicates homology
MNHGGKIAMLGIPPQDVAVDWNQVIFK + Deamidated (NQ); 2 Oxidation (M)
1430 580.3622 1158.7098 1158.7046 4.54 1 5 0.65 +1Score > 31 indicates identity
Score > 15 indicates homology
SLPLLMITKK + Oxidation (M)
1984 711.8276 1421.6406 1421.6344 4.40 1 5 0.77 +1Score > 40 indicates identity
Score > 16 indicates homology
QAVNDAELMADTK + Deamidated (NQ); Oxidation (M)
2823 608.3158 1821.9256 1821.9077 9.82 1 5 1 +1Score > 40 indicates identity
Score > 17 indicates homology
ALTMSDRIAVMSDGAIR + Oxidation (M)
2603 855.4288 1708.8430 1708.8342 5.20 0 5 0.98 +1Score > 41 indicates identity
Score > 17 indicates homology
FANAGVQAIICTDISK + 2 Deamidated (NQ)
3761 604.8404 2415.3325 2415.2944 15.8 1 5 0.45 +1Score > 33 indicates identity
Score > 14 indicates homology
ANMIIPQIADNLTRSGHLPIPK + Deamidated (NQ); Oxidation (M)
3802 819.0569 2454.1489 2454.1656 -6.82 1 5 0.9 +1Score > 38 indicates identity
Score > 17 indicates homology
RNSNNGVTTSSNTISTSAMIAGIR + 3 Deamidated (NQ)
4055 900.4827 2698.4263 2698.4707 -16.5 1 5 0.6 +1Score > 35 indicates identity
Score > 15 indicates homology
KLALFGGSYDPVHLGHLLLADAVHR
4287 724.3702 2893.4517 2893.4506 0.37 1 5 1 +1Score > 37 indicates identity
Score > 17 indicates homology
LAYCLQKCNVPLFVQHDAAQLYLK + 2 Deamidated (NQ)
3372 1084.5723 2167.1300 2167.1412 -5.16 1 5 1 +1Score > 38 indicates identity
Score > 17 indicates homology
VIDFLKSYNLVNDSVLAQK + 2 Deamidated (NQ)
3409 728.0587 2181.1543 2181.1641 -4.50 1 5 0.38 +1Score > 37 indicates identity
Score > 13 indicates homology
ATTPLTAATSNPSTPAKPAKQK + Deamidated (NQ)
4362 747.6663 2986.6361 2986.6170 6.40 0 5 1 +1Score > 31 indicates identity
Score > 17 indicates homology
MRPILVATTLMLLHNLQFLTSAIMAR + Deamidated (NQ); 2 Oxidation (M)
971 947.4949 946.4876 946.4720 16.5 1 5 0.8 +1Score > 42 indicates identity
Score > 16 indicates homology
SGDIETIGR
3045 991.4655 1980.9164 1980.9164 0.026 1 5 0.78 +1Score > 39 indicates identity
Score > 16 indicates homology
VQNIGKTSIDDVNVSDFE + 2 Deamidated (NQ)
3407 546.2838 2181.1061 2181.1277 -9.91 1 5 0.38 +1Score > 39 indicates identity
Score > 13 indicates homology
LTNLGLVTVSLASGLGQHRDE + 2 Deamidated (NQ)
3821 824.4312 2470.2718 2470.2380 13.7 0 5 0.93 +1Score > 37 indicates identity
Score > 17 indicates homology
LSIPTQDNFYIDLIDDPHVVR + Deamidated (NQ)
1034 973.5053 972.4980 972.5062 -8.43 1 5 0.88 +1Score > 42 indicates identity
Score > 17 indicates homology
KNVMQVPR + 2 Deamidated (NQ)
2943 957.9789 1913.9432 1913.9285 7.71 0 5 0.82 +1Score > 40 indicates identity
Score > 16 indicates homology
NHPFFGAINWNSIQNR
3222 698.3821 2092.1245 2092.1528 -13.5 1 5 0.6 +1Score > 36 indicates identity
Score > 15 indicates homology
DGLITPTIAPNGAQVLQVKR + 2 Deamidated (NQ)
4560 662.5681 3307.8041 3307.7964 2.33 1 5 1 +1Score > 31 indicates identity
Score > 17 indicates homology
VLYILLLVVGFSLMCLELFHSSNVIDGLK + Oxidation (M)
3377 543.5506 2170.1733 2170.1676 2.62 0 5 0.64 +1Score > 36 indicates identity
Score > 15 indicates homology
LLGTAMIMMPVTTAGINALPR
3445 734.7084 2201.1034 2201.0925 4.92 0 5 0.79 +1Score > 39 indicates identity
Score > 16 indicates homology
LSPGGSADLLAITWLLAQMNE + 2 Deamidated (NQ)
3634 773.3961 2317.1665 2317.2127 -19.9 0 5 0.55 +1Score > 38 indicates identity
Score > 14 indicates homology
IAPSDILVIYDDMDLPIGTIK + Oxidation (M)
1482 591.3671 1180.7196 1180.7179 1.46 1 5 0.56 +1Score > 32 indicates identity
Score > 15 indicates homology
KIPNLLDLQK
3406 1091.5591 2181.1036 2181.0774 12.0 1 5 0.94 +1Score > 39 indicates identity
Score > 17 indicates homology
VNEPTAVNVAAVDGRGRPNSR + 3 Deamidated (NQ)
3450 735.3850 2203.1332 2203.1194 6.23 1 5 0.6 +1Score > 38 indicates identity
Score > 15 indicates homology
ASPAQIAAFAVAMQVKVPTSAE + Deamidated (NQ); Oxidation (M)
2173 505.6086 1513.8040 1513.7848 12.6 1 5 0.9 +1Score > 40 indicates identity
Score > 17 indicates homology
GNTVQAKNANAGVLR + 2 Deamidated (NQ)
3812 822.1146 2463.3220 2463.3009 8.55 0 5 0.62 +1Score > 35 indicates identity
Score > 15 indicates homology
IAVAHYAAQLSTLDINPQPLSLE
4191 703.1405 2808.5329 2808.5421 -3.27 0 5 0.74 +1Score > 32 indicates identity
Score > 16 indicates homology
SLGQFLLQILGMLSGVGIMLLIALYE + Deamidated (NQ); Oxidation (M)
2302 524.6437 1570.9093 1570.8831 16.6 0 5 1 +1Score > 33 indicates identity
Score > 17 indicates homology
NLLAGGLGIDNLFVR
3724 794.7590 2381.2552 2381.2083 19.7 1 5 0.67 +1Score > 36 indicates identity
Score > 15 indicates homology
DTALVVHLGMSGQMLLGAVPRAE + Deamidated (NQ); Oxidation (M)
4571 1110.5620 3328.6642 3328.6301 10.2 1 5 0.42 +1Score > 35 indicates identity
Score > 13 indicates homology
VLSPKPGFNRWVATLDFNALYPTIMMWE + Deamidated (NQ); 2 Oxidation (M)
3522 751.0879 2250.2419 2250.2008 18.2 0 5 1 +1Score > 33 indicates identity
Score > 17 indicates homology
GAPAVPANFLRPIIQADLDSGK + Deamidated (NQ)
3756 805.1288 2412.3646 2412.3952 -12.7 1 5 0.68 +1Score > 30 indicates identity
Score > 15 indicates homology
IVSIVGTSGAGKTTLLQILGTLDR
3162 684.3934 2050.1584 2050.1323 12.7 1 5 1 +1Score > 32 indicates identity
Score > 17 indicates homology
RDWPGVSAVLAAIALDLQR
3787 813.7906 2438.3500 2438.3169 13.6 1 5 0.85 +1Score > 32 indicates identity
Score > 16 indicates homology
ITVDVDLGDRAYPIHIGSGLLSK
3531 564.3009 2253.1745 2253.1364 16.9 1 5 1 +1Score > 38 indicates identity
Score > 17 indicates homology
LAGMIGGQAIRYDVWGNYLR + Deamidated (NQ)
4063 676.8464 2703.3565 2703.3399 6.15 1 5 0.8 +1Score > 37 indicates identity
Score > 16 indicates homology
GFGPNGAMVIVDALTNNVNRTASDVR + Oxidation (M)
1664 633.8117 1265.6088 1265.6112 -1.89 1 4 0.61 +1Score > 41 indicates identity
Score > 15 indicates homology
EQAVAAAQQGHR + Deamidated (NQ)
3563 759.0776 2274.2110 2274.2107 0.14 1 4 0.85 +1Score > 36 indicates identity
Score > 16 indicates homology
KISDIPAYNSINGTALIQNLK + 2 Deamidated (NQ)
1467 586.8536 1171.6926 1171.6826 8.60 1 4 1 +1Score > 36 indicates identity
Score > 17 indicates homology
YVNVRGVPLR
1924 697.8881 1393.7616 1393.7453 11.8 1 4 0.94 +1Score > 37 indicates identity
Score > 17 indicates homology
VAPLIEPLRQQE + 2 Deamidated (NQ)
3254 529.5413 2114.1361 2114.1120 11.4 1 4 0.74 +1Score > 37 indicates identity
Score > 16 indicates homology
QDKQNLFAIIQGGLNADLR + Deamidated (NQ)
3373 723.7325 2168.1757 2168.1657 4.62 0 4 1 +1Score > 35 indicates identity
Score > 17 indicates homology
DYLSLTIDLPLIFGFSLIE
4006 885.4437 2653.3093 2653.2792 11.3 1 4 0.55 +1Score > 38 indicates identity
Score > 14 indicates homology
MSQAQINTLASLFDSAIAVLKNDGE + 2 Deamidated (NQ); Oxidation (M)
2700 877.4647 1752.9148 1752.8934 12.2 1 4 0.78 +1Score > 39 indicates identity
Score > 16 indicates homology
LSPETLYLAVNYVDR + Deamidated (NQ)
3727 795.7154 2384.1244 2384.1583 -14.2 1 4 0.51 +1Score > 38 indicates identity
Score > 14 indicates homology
TMVVSYRVPHNAAVQVYDYR + Deamidated (NQ); Oxidation (M)
2862 928.4910 1854.9674 1854.9475 10.7 1 4 1 +1Score > 39 indicates identity
Score > 17 indicates homology
QLLADGGHAILAEVYQR + 2 Deamidated (NQ)
2934 636.2834 1905.8284 1905.8488 -10.7 0 4 0.4 +1Score > 36 indicates identity
Score > 13 indicates homology
TLQDMMNIIDYNQYK + Deamidated (NQ); Oxidation (M)
3350 718.7476 2153.2210 2153.1804 18.8 1 4 1 +1Score > 31 indicates identity
Score > 17 indicates homology
RGNQVIIAGDGQVSLGQTVIK + Deamidated (NQ)
3417 1094.5314 2187.0482 2187.0782 -13.7 1 4 0.88 +1Score > 39 indicates identity
Score > 16 indicates homology
LNTLHNLHYYQELMAGLR + 2 Deamidated (NQ)
3573 760.3981 2278.1725 2278.2072 -15.2 0 4 0.68 +1Score > 38 indicates identity
Score > 15 indicates homology
SSAALFVSFMINLFVTAVFAK + Oxidation (M)
2592 853.9311 1705.8476 1705.8432 2.59 1 4 0.57 +1Score > 41 indicates identity
Score > 15 indicates homology
WGNMILKWLSDMGR
2968 966.0400 1930.0654 1930.0775 -6.24 1 4 0.4 +1Score > 35 indicates identity
Score > 13 indicates homology
LLSNPLLQTYLPNSTKK + Deamidated (NQ)
3617 770.3742 2308.1008 2308.1007 0.028 1 4 0.56 +1Score > 39 indicates identity
Score > 14 indicates homology
GITAVLFVLAMSEYDQMLFE + 2 Oxidation (M)
1912 463.9352 1388.7838 1388.7888 -3.62 1 4 0.73 +1Score > 37 indicates identity
Score > 16 indicates homology
AVRAATYGGALALR
2737 885.9937 1769.9728 1769.9563 9.33 0 4 0.54 +1Score > 37 indicates identity
Score > 14 indicates homology
VLSDLVPNQLYNLPGK + Deamidated (NQ)
3794 490.2377 2446.1521 2446.1685 -6.72 1 4 0.69 +1Score > 38 indicates identity
Score > 15 indicates homology
SSELYIQGTHNIANALACLALGE + 2 Deamidated (NQ)
3835 827.4289 2479.2649 2479.2819 -6.88 1 4 0.39 +1Score > 38 indicates identity
Score > 13 indicates homology
QPTHPLPAPGGGDLLRAVQVSDPR + 2 Deamidated (NQ)
4250 953.8101 2858.4085 2858.4630 -19.1 1 4 1 +1Score > 37 indicates identity
Score > 17 indicates homology
NIVAGLQALGVNALGLTGADMNLMRSDK + Deamidated (NQ); Oxidation (M)
4415 1021.5273 3061.5601 3061.5204 13.0 1 4 1 +1Score > 36 indicates identity
Score > 17 indicates homology
GSVTAGNASGINDGAAAVLIASDEAVAQYQLK + Deamidated (NQ)
2475 823.4478 1644.8810 1644.8518 17.8 1 4 0.68 +1Score > 39 indicates identity
Score > 15 indicates homology
IGQWMRTAAALQAGR + Oxidation (M)
2958 962.4541 1922.8936 1922.8904 1.67 1 4 0.71 +1Score > 40 indicates identity
Score > 15 indicates homology
YRQADSACAVDALDAAAR
3880 842.1391 2523.3955 2523.3544 16.3 1 4 0.61 +1Score > 32 indicates identity
Score > 15 indicates homology
AALADVVLLQADVTADGADDRALLK
2576 567.6438 1699.9096 1699.9331 -13.9 1 4 0.87 +1Score > 39 indicates identity
Score > 16 indicates homology
FLGKTVILTTGTFMR + Oxidation (M)
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Not what you expected? Try the select summary.