MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2944 638.9886 1913.9440 1913.9418 1.15 1 7 0.92 +1Score > 40 indicates identity
Score > 19 indicates homology
NWLMQGVAAQGDRVQLE
2960 642.3100 1923.9082 1923.9115 -1.75 1 7 0.6 +1Score > 40 indicates identity
Score > 17 indicates homology
QADNPFSWKNFVDTVR + Deamidated (NQ)
3497 747.0459 2238.1159 2238.1579 -18.8 1 7 0.73 +1Score > 39 indicates identity
Score > 18 indicates homology
LCDWVPALLSGTQAPADIRR
364 341.6984 681.3822 681.3922 -14.6 0 7 0.97 +1Score > 37 indicates identity
Score > 19 indicates homology
IGHVTR
3645 775.4182 2323.2328 2323.2073 11.0 1 7 0.33 +1Score > 36 indicates identity
Score > 14 indicates homology
NHLTVTWKVSAGIYQHVDVR + Deamidated (NQ)
3675 783.4286 2347.2640 2347.2933 -12.5 1 7 0.71 +1Score > 35 indicates identity
Score > 18 indicates homology
AQEPVKGPVSTKPGSCPIILIR + Deamidated (NQ)
2595 853.9886 1705.9626 1705.9363 15.5 1 7 0.3 +1Score > 35 indicates identity
Score > 14 indicates homology
SPIVEPGLEALLQQGR
1682 638.8163 1275.6180 1275.6017 12.8 1 7 0.92 +1Score > 41 indicates identity
Score > 19 indicates homology
DIVVAVTGENME
1853 679.8972 1357.7798 1357.7605 14.2 1 7 0.88 +1Score > 36 indicates identity
Score > 19 indicates homology
IADKFAGLPADIK
2107 741.9152 1481.8158 1481.7950 14.0 1 7 0.67 +1Score > 38 indicates identity
Score > 18 indicates homology
AHTRVDLVNSATAK
1926 698.8441 1395.6736 1395.6994 -18.4 0 7 0.65 +1Score > 40 indicates identity
Score > 17 indicates homology
VNLDHTNIDINK + Deamidated (NQ)
2153 753.9357 1505.8568 1505.8752 -12.2 0 7 0.56 +1Score > 35 indicates identity
Score > 17 indicates homology
LLDLLHLQLICR
3269 1060.5493 2119.0840 2119.0586 12.0 1 7 1 +1Score > 39 indicates identity
Score > 19 indicates homology
QFLILNDFPSVNEAIDQR + Deamidated (NQ)
3765 806.7453 2417.2141 2417.1751 16.1 1 7 0.7 +1Score > 38 indicates identity
Score > 18 indicates homology
DFLNAGGDIVISGLPQWETDLR + 2 Deamidated (NQ)
3854 833.4629 2497.3669 2497.3216 18.1 1 7 0.63 +1Score > 33 indicates identity
Score > 17 indicates homology
AEIAGPGFINLTLKPQVWSDALR + 2 Deamidated (NQ)
3125 678.3391 2031.9955 2031.9983 -1.37 1 7 0.64 +1Score > 39 indicates identity
Score > 17 indicates homology
YAAAVVHAVQCVCDRSVK
3567 759.3920 2275.1542 2275.1743 -8.85 1 7 0.42 +1Score > 39 indicates identity
Score > 15 indicates homology
QALVTTQGRTPAMVQPAVPHR + 2 Deamidated (NQ); Oxidation (M)
3690 590.5579 2358.2025 2358.1831 8.24 0 7 0.78 +1Score > 38 indicates identity
Score > 18 indicates homology
QPWGVAAVITPWNFPSAMITR + Deamidated (NQ); Oxidation (M)
2830 609.3366 1824.9880 1824.9621 14.2 0 7 0.65 +1Score > 37 indicates identity
Score > 17 indicates homology
ASVLFNIGALYTQIGTR + 2 Deamidated (NQ)
3402 728.0392 2181.0958 2181.1277 -14.6 1 7 0.49 +1Score > 39 indicates identity
Score > 16 indicates homology
LTNLGLVTVSLASGLGQHRDE + 2 Deamidated (NQ)
3824 825.0978 2472.2716 2472.2536 7.25 1 7 0.49 +1Score > 37 indicates identity
Score > 16 indicates homology
VSFTTTDLSQPLASSYNPLFKR + Deamidated (NQ)
4059 901.7997 2702.3773 2702.3360 15.3 1 7 0.63 +1Score > 37 indicates identity
Score > 17 indicates homology
SIVADSWLAAMQDVLKVAVQSDIPE + 2 Deamidated (NQ); Oxidation (M)
4190 937.1592 2808.4558 2808.4003 19.7 0 7 0.39 +1Score > 36 indicates identity
Score > 15 indicates homology
LMIDLPADSAFLQANLHPSNLDAAIR + 3 Deamidated (NQ)
2003 477.9105 1430.7097 1430.7306 -14.7 0 7 0.73 +1Score > 41 indicates identity
Score > 18 indicates homology
SKPLFTFSHPNR + Deamidated (NQ)
2676 581.6465 1741.9177 1741.9403 -13.0 0 7 0.86 +1Score > 39 indicates identity
Score > 19 indicates homology
YAIIFNLLAHKPNVE + Deamidated (NQ)
2188 507.6128 1519.8166 1519.8035 8.61 1 7 0.88 +1Score > 39 indicates identity
Score > 19 indicates homology
GVLIKDFFDGNAPK
3853 832.7767 2495.3083 2495.3092 -0.37 1 7 0.56 +1Score > 37 indicates identity
Score > 17 indicates homology
AVNQAPSSQIDSALAGDGLSRLVAR
871 908.4646 907.4573 907.4624 -5.60 1 7 0.28 +1Score > 42 indicates identity
Score > 14 indicates homology
GVSYRGNR
2784 901.9973 1801.9800 1801.9747 2.98 1 7 0.38 +1Score > 37 indicates identity
Score > 15 indicates homology
MSLLEAIVLGLVQGLTE + Deamidated (NQ); Oxidation (M)
3726 795.4015 2383.1827 2383.1631 8.23 1 7 0.3 +1Score > 39 indicates identity
Score > 14 indicates homology
GNATIEQMLPTLPPFWHSATR + Deamidated (NQ); Oxidation (M)
4767 1012.9808 4047.8941 4047.8632 7.64 1 7 0.74 +1Score > 32 indicates identity
Score > 18 indicates homology
LQHQWDAISGMPTNLYGQNDNFHPENSHVLPALMR + 2 Deamidated (NQ); Oxidation (M)
2549 559.2932 1674.8578 1674.8399 10.7 1 7 0.89 +1Score > 40 indicates identity
Score > 19 indicates homology
RAYIDYSMSVIVSR + Oxidation (M)
2636 862.4744 1722.9342 1722.9563 -12.8 1 7 0.74 +1Score > 37 indicates identity
Score > 18 indicates homology
MIRLGVNIDHVATLR + Oxidation (M)
2703 878.4250 1754.8354 1754.8120 13.4 1 7 0.37 +1Score > 40 indicates identity
Score > 15 indicates homology
HIMLTQAEQCSFFK + Oxidation (M)
3984 877.4516 2629.3330 2629.3230 3.79 1 7 0.44 +1Score > 37 indicates identity
Score > 16 indicates homology
NITLSMLISNNILNSITSIKMFE + 2 Deamidated (NQ); 2 Oxidation (M)
2277 781.4357 1560.8568 1560.8399 10.8 0 7 0.54 +1Score > 38 indicates identity
Score > 16 indicates homology
QFSLLLADTVLVNE
3434 732.3900 2194.1482 2194.1270 9.66 1 7 1 +1Score > 38 indicates identity
Score > 19 indicates homology
DLFLYSNALVQDAESQILR
3678 784.0605 2349.1597 2349.1826 -9.75 1 7 0.92 +1Score > 39 indicates identity
Score > 19 indicates homology
TGPAGAPGPPGAPGAPGPVGPAGKSGDR
3893 845.4891 2533.4455 2533.4681 -8.93 1 7 0.41 +1Score > 29 indicates identity
Score > 15 indicates homology
WPPFLLGCTILVILLVMKHVGK
1996 714.4247 1426.8348 1426.8143 14.4 1 7 0.49 +1Score > 34 indicates identity
Score > 16 indicates homology
IQQLAVSGLKVNR + 2 Deamidated (NQ)
2629 574.6351 1720.8835 1720.8519 18.3 1 7 0.6 +1Score > 40 indicates identity
Score > 17 indicates homology
QAENITTVGQAIAYLE + Deamidated (NQ)
3283 709.3784 2125.1134 2125.0837 13.9 1 7 0.32 +1Score > 38 indicates identity
Score > 14 indicates homology
GPVQGNLDPMRLVAGGQALSK + 2 Deamidated (NQ); Oxidation (M)
3128 679.0300 2034.0682 2034.0707 -1.25 1 7 0.87 +1Score > 38 indicates identity
Score > 18 indicates homology
TLGLLQLMAQAGLFITAEE + Oxidation (M)
4002 882.8145 2645.4217 2645.3961 9.66 0 7 1 +1Score > 34 indicates identity
Score > 19 indicates homology
GKPMGIALGGLAMGVLVGPPFGSVLYE + Oxidation (M)
2077 735.4148 1468.8150 1468.7960 13.0 1 7 0.82 +1Score > 38 indicates identity
Score > 18 indicates homology
SKCVVLTTGTYLK
2254 517.6060 1549.7962 1549.7963 -0.089 0 7 0.78 +1Score > 40 indicates identity
Score > 18 indicates homology
FGGSMTLHIVPFTK + Oxidation (M)
2997 972.0363 1942.0580 1942.0789 -10.7 0 7 0.34 +1Score > 36 indicates identity
Score > 14 indicates homology
VGTPQLLALVQHWVPGAR + Deamidated (NQ)
3957 648.8072 2591.1997 2591.2214 -8.37 1 7 0.37 +1Score > 37 indicates identity
Score > 15 indicates homology
VWTAVANHVESGIVAAFTMGDLSAE + Deamidated (NQ); Oxidation (M)
4179 934.7952 2801.3638 2801.3429 7.45 1 7 0.54 +1Score > 38 indicates identity
Score > 16 indicates homology
DQHQIILTADLTSGAQVNDLYKMPE + 2 Deamidated (NQ)
2861 927.9550 1853.8954 1853.8942 0.70 0 7 0.83 +1Score > 40 indicates identity
Score > 18 indicates homology
QAGSLAAAYSGMGAVSGNVK + Oxidation (M)
3115 677.0240 2028.0502 2028.0198 15.0 0 7 0.8 +1Score > 39 indicates identity
Score > 18 indicates homology
LLTGNGTDQTHLLVVMTAE + Oxidation (M)
1309 554.2993 1106.5840 1106.5945 -9.40 1 7 0.81 +1Score > 40 indicates identity
Score > 18 indicates homology
QPPRPNNRK + Deamidated (NQ)
2393 806.4387 1610.8628 1610.8416 13.2 0 7 0.88 +1Score > 38 indicates identity
Score > 19 indicates homology
SVHNTFLTNILSHK + Deamidated (NQ)
3572 760.0582 2277.1528 2277.1324 8.95 1 7 0.5 +1Score > 39 indicates identity
Score > 16 indicates homology
NPQSVNIHCLKAHSPLASFR + 2 Deamidated (NQ)
1084 333.2074 996.6004 996.6008 -0.41 1 7 0.99 +1Score > 35 indicates identity
Score > 19 indicates homology
KSLVFYLK
2656 867.0336 1732.0526 1732.0359 9.67 0 7 0.68 +1Score > 25 indicates identity
Score > 17 indicates homology
NLIILINHLTTRPSK
3280 1061.5745 2121.1344 2121.0929 19.6 0 7 0.52 +1Score > 36 indicates identity
Score > 16 indicates homology
RPATVFVASFIGSPGMNLLE + Oxidation (M)
4187 936.8467 2807.5183 2807.4720 16.5 1 7 1 +1Score > 33 indicates identity
Score > 19 indicates homology
FYNLMIENVFLLILQTIWQHQK + Deamidated (NQ); Oxidation (M)
2910 943.4999 1884.9852 1884.9839 0.69 1 7 0.82 +1Score > 39 indicates identity
Score > 18 indicates homology
NQVNQDLMIAGIRSGLR + Deamidated (NQ)
3048 662.0031 1982.9875 1982.9850 1.25 0 7 0.93 +1Score > 40 indicates identity
Score > 19 indicates homology
VNFAVQAFAALNSNNFVR + 2 Deamidated (NQ)
4816 865.4489 4322.2081 4322.1796 6.60 0 7 0.51 +1Score > 31 indicates identity
Score > 16 indicates homology
SVNSAIVASMGFLIIWICYFGVNLLGIGLHSYGSFTLTSN + Deamidated (NQ)
3298 1067.5746 2133.1346 2133.1028 15.0 1 6 0.66 +1Score > 37 indicates identity
Score > 17 indicates homology
MGLVDQLVEPLGPGLKPPEE + Oxidation (M)
226 303.1786 604.3426 604.3544 -19.5 1 6 0.81 +1Score > 37 indicates identity
Score > 18 indicates homology
TTAKGK
551 384.7070 767.3994 767.3926 8.93 1 6 0.73 +1Score > 37 indicates identity
Score > 18 indicates homology
HLQGGEK
2717 588.2868 1761.8386 1761.8463 -4.39 1 6 0.59 +1Score > 40 indicates identity
Score > 17 indicates homology
SASATMAFIMMKNSLK + 2 Oxidation (M)
4142 927.4664 2779.3774 2779.3586 6.76 1 6 0.4 +1Score > 38 indicates identity
Score > 15 indicates homology
AIALGAQNVDVNVQGAFTGDISTEMLK + 2 Deamidated (NQ); Oxidation (M)
4272 962.8085 2885.4037 2885.3793 8.45 1 6 0.56 +1Score > 37 indicates identity
Score > 16 indicates homology
TVFNVLGPLTNPANANYQLMGVYDEK + 2 Deamidated (NQ); Oxidation (M)
4354 744.3827 2973.5017 2973.5059 -1.41 1 6 0.59 +1Score > 36 indicates identity
Score > 17 indicates homology
HGLYDDIWQAFAVLLPVKTVGVMGDGR + Deamidated (NQ); Oxidation (M)
2368 532.9445 1595.8117 1595.8096 1.30 0 6 0.67 +1Score > 40 indicates identity
Score > 17 indicates homology
VAIYVNGNYQWLR + Deamidated (NQ)
3416 1094.5157 2187.0168 2187.0253 -3.86 1 6 0.42 +1Score > 39 indicates identity
Score > 15 indicates homology
AVDPDDIEMLQDLVIAATNE + Oxidation (M)
3023 656.9880 1967.9422 1967.9809 -19.7 1 6 0.89 +1Score > 40 indicates identity
Score > 18 indicates homology
YTTEVIVNVGGTMQMLGR
4495 1061.5240 3181.5502 3181.5635 -4.19 1 6 0.87 +1Score > 36 indicates identity
Score > 18 indicates homology
EGAQQMGLQLMDSPTPIQPIVIGDSAQALR + 2 Deamidated (NQ); Oxidation (M)
2673 581.6444 1741.9114 1741.9403 -16.6 0 6 0.72 +1Score > 39 indicates identity
Score > 18 indicates homology
YAIIFNLLAHKPNVE + Deamidated (NQ)
3084 1002.5472 2003.0798 2003.0575 11.2 1 6 0.91 +1Score > 36 indicates identity
Score > 19 indicates homology
AGVVSDVGGIEQVNYILIR + 2 Deamidated (NQ)
3478 743.0607 2226.1603 2226.1930 -14.7 1 6 0.6 +1Score > 38 indicates identity
Score > 17 indicates homology
ISGVMGVVESDAQLQIILGPGK + Oxidation (M)
3929 856.4429 2566.3069 2566.2598 18.3 0 6 0.69 +1Score > 37 indicates identity
Score > 17 indicates homology
ATGPAFSIVCPGIRPGGPVADDQQR + Deamidated (NQ)
2212 510.9532 1529.8378 1529.8202 11.5 1 6 0.98 +1Score > 39 indicates identity
Score > 19 indicates homology
GEFDAIILAAAGINR
2837 919.0109 1836.0072 1836.0257 -10.1 0 6 0.5 +1Score > 36 indicates identity
Score > 16 indicates homology
ALALTWLQGALAQAPQGK
1755 437.2636 1308.7690 1308.7666 1.80 1 6 0.51 +1Score > 33 indicates identity
Score > 16 indicates homology
DIPKPLRFPAR
3270 707.3859 2119.1359 2119.0959 18.9 0 6 0.86 +1Score > 37 indicates identity
Score > 18 indicates homology
KPGVVLGMGGYVTVPGGWMAK + Oxidation (M)
1725 324.9384 1295.7245 1295.6986 20.0 1 6 0.93 +1Score > 37 indicates identity
Score > 19 indicates homology
SRYQDILVFR
2575 567.6356 1699.8850 1699.8716 7.88 1 6 0.63 +1Score > 40 indicates identity
Score > 17 indicates homology
QGISFMGIHVKVDPR + Deamidated (NQ); Oxidation (M)
2957 641.6871 1922.0395 1922.0585 -9.91 0 6 0.37 +1Score > 36 indicates identity
Score > 15 indicates homology
LAQQAGGGLIAQAVDINGVK
3670 1173.1859 2344.3572 2344.3188 16.4 1 6 0.3 +1Score > 27 indicates identity
Score > 14 indicates homology
VLRQATLQVTIIPILCGSSFK + Deamidated (NQ)
3538 754.0754 2259.2044 2259.2144 -4.45 1 6 0.83 +1Score > 36 indicates identity
Score > 18 indicates homology
QADGSAVVKLGNAMILATVVSAK + Deamidated (NQ); Oxidation (M)
3655 777.4473 2329.3201 2329.2906 12.6 1 6 0.32 +1Score > 30 indicates identity
Score > 14 indicates homology
SSLGSIATKFNFLIHNLAQLR
3980 875.8227 2624.4463 2624.4650 -7.12 1 6 0.26 +1Score > 32 indicates identity
Score > 13 indicates homology
SIGQLKLQVPAGAATPSPPIGPALGQR + Deamidated (NQ)
140 485.2742 484.2669 484.2645 4.92 0 6 0.58 +1Score > 32 indicates identity
Score > 16 indicates homology
SPPGK
2658 867.4785 1732.9424 1732.9586 -9.30 1 6 0.94 +1Score > 37 indicates identity
Score > 19 indicates homology
KLPLYLSNVWQMIK + Deamidated (NQ)
3564 759.3483 2275.0231 2275.0024 9.09 1 6 0.69 +1Score > 37 indicates identity
Score > 17 indicates homology
LILSNQYYLMSQMDPENAE + Deamidated (NQ); Oxidation (M)
4575 1114.6078 3340.8016 3340.7554 13.8 1 6 0.41 +1Score > 31 indicates identity
Score > 15 indicates homology
ILSAIDYLHSLEPPIIHGNINAKNILLDNE + 2 Deamidated (NQ)
3560 758.4121 2272.2145 2272.1997 6.48 1 6 0.67 +1Score > 36 indicates identity
Score > 17 indicates homology
KSNFSLAILNVGAPAAGMNAAVR + Deamidated (NQ)
3081 668.0265 2001.0577 2001.0565 0.60 1 6 0.5 +1Score > 39 indicates identity
Score > 16 indicates homology
DTLIVSAVTPDLNMTRQK
2312 394.4914 1573.9365 1573.9079 18.2 1 6 0.47 +1Score > 30 indicates identity
Score > 15 indicates homology
SLAAILLEVFSVANK
276 635.3539 634.3466 634.3438 4.39 0 6 0.8 +1Score > 41 indicates identity
Score > 18 indicates homology
IYSPR
2869 929.9764 1857.9382 1857.9724 -18.4 0 6 0.94 +1Score > 40 indicates identity
Score > 19 indicates homology
TQVILQLQPVQQGIYE + 2 Deamidated (NQ)
3046 991.4880 1980.9614 1980.9826 -10.7 0 6 0.99 +1Score > 40 indicates identity
Score > 19 indicates homology
GQQGIGISAAVLYAQMTAGK + 2 Deamidated (NQ); Oxidation (M)
3182 1030.5374 2059.0602 2059.0884 -13.7 1 6 0.65 +1Score > 39 indicates identity
Score > 17 indicates homology
ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
2426 810.9174 1619.8202 1619.8454 -15.5 1 6 0.84 +1Score > 40 indicates identity
Score > 18 indicates homology
QHPDMSVTKVVVHK + Oxidation (M)
2771 900.9728 1799.9310 1799.9128 10.1 1 6 0.93 +1Score > 40 indicates identity
Score > 18 indicates homology
QVDGFTASITGKMFAVK + Deamidated (NQ)
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