MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1333 561.3029 1120.5912 1120.5877 3.20 0 30 0.33 +1Score > 41 indicates identity
Score > 37 indicates homology		 NYTIGDVIAR
1832 449.2385 1344.6937 1344.6959 -1.64 1 30 0.076 +1Score > 41 indicates identity
Score > 31 indicates homology		 AIDLLGSKTAPME
172 530.3325 529.3252 529.3224 5.39 0 30 0.92 +1Score > 47 indicates identity
Score > 42 indicates homology		 AAAIGK
639 403.7256 805.4366 805.4334 4.06 1 29 0.09 +1Score > 41 indicates identity
Score > 32 indicates homology		 AAVAFKAE
1096 502.2588 1002.5030 1002.4982 4.87 0 29 0.35 +1Score > 42 indicates identity
Score > 37 indicates homology		 IANISGDGQK + Deamidated (NQ)
1980 474.5760 1420.7062 1420.6809 17.8 1 29 0.17 +1Score > 41 indicates identity
Score > 34 indicates homology		 YWPSIPGMGEIR + Oxidation (M)
1194 521.2926 1040.5706 1040.5727 -1.94 1 29 0.32 +1Score > 39 indicates identity
Score > 37 indicates homology		 GGSIRNAIPR + Deamidated (NQ)
2184 506.9543 1517.8411 1517.8202 13.8 1 29 0.12 +1Score > 37 indicates identity
Score > 33 indicates homology		 VKFNVINTAVQGAR + 2 Deamidated (NQ)
713 423.2284 844.4422 844.4290 15.7 1 29 0.46 +1Score > 43 indicates identity
Score > 38 indicates homology		 AQAELDAK
1406 384.5412 1150.6018 1150.5870 12.9 0 29 0.14 +1Score > 41 indicates identity
Score > 33 indicates homology		 ASNLQDLVYK + Deamidated (NQ)
4247 953.1583 2856.4531 2856.4123 14.3 1 29 0.31 +1Score > 37 indicates identity VKDTNGVYVVPAFTGLGAPYWDPYAR + Deamidated (NQ)
481 740.4439 739.4366 739.4341 3.46 0 29 0.19 +1Score > 35 indicates identity
Score > 34 indicates homology		 AVAVAGPR
1938 467.9244 1400.7514 1400.7762 -17.8 1 29 0.083 +1Score > 40 indicates identity
Score > 31 indicates homology		 TIDETLDLLLAGK
1948 702.8460 1403.6774 1403.6908 -9.48 0 29 0.38 +1Score > 41 indicates identity
Score > 37 indicates homology		 IHPYGMFVLADAA
798 440.2527 878.4908 878.4861 5.37 0 29 0.47 +1Score > 38 indicates identity
Score > 38 indicates homology		 YILNSIR + Deamidated (NQ)
399 350.7226 699.4306 699.4279 3.93 0 29 0.26 +1Score > 41 indicates identity
Score > 36 indicates homology		 AAGAIGLK
1554 608.8287 1215.6428 1215.6282 12.1 1 29 0.3 +1Score > 41 indicates identity
Score > 36 indicates homology		 VAMSIEGPTVGR
764 432.2357 862.4568 862.4548 2.32 0 29 0.13 +1Score > 42 indicates identity
Score > 33 indicates homology		 AFAAGADIK
326 331.6908 661.3670 661.3693 -3.46 1 29 0.31 +1Score > 41 indicates identity
Score > 36 indicates homology		 ISMRR
354 675.3438 674.3365 674.3347 2.64 0 29 0.73 +1Score > 44 indicates identity
Score > 40 indicates homology		 DINTGR
799 440.2530 878.4914 878.4861 6.05 0 29 0.41 +1Score > 38 indicates identity
Score > 37 indicates homology		 YILNSIR + Deamidated (NQ)
633 401.7258 801.4370 801.4344 3.24 0 29 0.35 +1Score > 43 indicates identity
Score > 37 indicates homology		 AGADSLLR
589 787.5143 786.5070 786.5004 8.48 0 29 0.073 +1Score > 33 indicates identity
Score > 30 indicates homology		 FAPIVLK
186 560.3176 559.3103 559.3078 4.48 0 29 0.36 +1Score > 42 indicates identity
Score > 37 indicates homology		 GVATGR
1083 499.3065 996.5984 996.5855 13.0 1 29 0.23 +1Score > 35 indicates identity EVIAIDLPK
411 351.7063 701.3980 701.4072 -13.0 0 29 0.91 +1Score > 43 indicates identity
Score > 41 indicates homology		 VGAGTAVK
2916 944.9969 1887.9792 1887.9537 13.5 1 29 0.25 +1Score > 39 indicates identity
Score > 35 indicates homology		 NSITDKNINNNITNIAK + 2 Deamidated (NQ)
1177 516.2621 1030.5096 1030.4931 16.1 0 28 0.25 +1Score > 42 indicates identity
Score > 35 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
1231 354.8590 1061.5552 1061.5506 4.35 1 28 0.099 +1Score > 41 indicates identity
Score > 31 indicates homology		 TPVEAQYVR
701 421.2496 840.4846 840.4817 3.47 1 28 0.16 +1Score > 37 indicates identity
Score > 33 indicates homology		 KITSGHAK
1586 615.3627 1228.7108 1228.7027 6.66 1 28 0.19 +1Score > 37 indicates identity
Score > 34 indicates homology		 QLSEGLLALSAK
2222 768.4116 1534.8086 1534.7813 17.8 1 28 0.063 +1Score > 40 indicates identity
Score > 29 indicates homology		 AQPIYIRTPSTCK + Deamidated (NQ)
722 423.7420 845.4694 845.4759 -7.67 0 28 0.8 +1Score > 42 indicates identity
Score > 40 indicates homology		 LPFGVSAR
1741 435.2605 1302.7597 1302.7395 15.5 0 28 0.077 +1Score > 34 indicates identity
Score > 30 indicates homology		 IVLVVNSGSSSIK + Deamidated (NQ)
874 455.7561 909.4976 909.4807 18.6 0 28 0.15 +1Score > 38 indicates identity
Score > 32 indicates homology		 IPLPNINE + Deamidated (NQ)
615 398.2233 794.4320 794.4286 4.30 0 28 0.26 +1Score > 39 indicates identity
Score > 35 indicates homology		 ASVSLYR
624 801.4606 800.4533 800.4504 3.64 1 28 0.61 +1Score > 43 indicates identity
Score > 38 indicates homology		 QIAANKR + Deamidated (NQ)
967 945.5026 944.4953 944.4927 2.80 0 28 0.89 +1Score > 43 indicates identity
Score > 40 indicates homology		 SQAAVAQAAK + Deamidated (NQ)
361 340.6586 679.3026 679.2926 14.9 0 28 0.36 +1Score > 38 indicates identity
Score > 36 indicates homology		 GDADFR
944 468.7866 935.5586 935.5480 11.3 0 28 0.099 +1Score > 34 indicates identity
Score > 31 indicates homology		 LGIPYVFK
510 377.1943 752.3740 752.3738 0.30 0 28 0.59 +1Score > 40 indicates identity
Score > 38 indicates homology		 MSTALSK + Oxidation (M)
391 347.6937 693.3728 693.3810 -11.7 0 28 0.23 +1Score > 40 indicates identity
Score > 34 indicates homology		 IFSATR
1578 614.8383 1227.6620 1227.6710 -7.30 1 28 0.6 +1Score > 40 indicates identity
Score > 38 indicates homology		 NILNEAQIIAK + 2 Deamidated (NQ)
724 848.3928 847.3855 847.3937 -9.60 1 28 0.51 +1Score > 41 indicates identity
Score > 37 indicates homology		 DDTWRR
1737 651.3547 1300.6948 1300.6874 5.70 0 28 0.74 +1Score > 41 indicates identity
Score > 39 indicates homology		 VLSDAIVDGANVK + Deamidated (NQ)
1118 337.8669 1010.5789 1010.5873 -8.29 0 28 0.42 +1Score > 36 indicates identity AAVDAALRPK
723 424.7000 847.3854 847.3937 -9.69 1 28 0.14 +1Score > 41 indicates identity
Score > 32 indicates homology		 DDTWRR
1471 587.7812 1173.5478 1173.5513 -2.95 1 28 0.25 +1Score > 40 indicates identity
Score > 34 indicates homology		 AGNELGAGQDLK + 2 Deamidated (NQ)
2306 787.4418 1572.8690 1572.8987 -18.9 1 28 0.18 +1Score > 37 indicates identity
Score > 33 indicates homology		 GKIFINSAIDNLLR
645 404.7155 807.4164 807.4126 4.71 0 28 0.16 +1Score > 42 indicates identity
Score > 32 indicates homology		 YAAVDAAK
2207 510.6184 1528.8334 1528.8249 5.52 0 27 0.24 +1Score > 39 indicates identity
Score > 34 indicates homology		 ADPVSIFGGVIALNR + Deamidated (NQ)
402 351.2206 700.4266 700.4232 4.94 0 27 0.43 +1Score > 41 indicates identity
Score > 36 indicates homology		 VVGGTIR
800 440.2533 878.4920 878.4861 6.74 0 27 0.47 +1Score > 38 indicates identity
Score > 37 indicates homology		 YILNSIR + Deamidated (NQ)
1584 615.3511 1228.6876 1228.6888 -0.91 1 27 0.49 +1Score > 40 indicates identity
Score > 37 indicates homology		 LSDGRVLLNSR
1870 685.3307 1368.6468 1368.6456 0.91 0 27 0.27 +1Score > 40 indicates identity
Score > 34 indicates homology		 HADQVLVMDQGR + Deamidated (NQ)
271 632.3756 631.3683 631.3653 4.77 0 27 0.36 +1Score > 41 indicates identity
Score > 35 indicates homology		 ATALTR
1182 345.5309 1033.5709 1033.5655 5.16 0 27 0.31 +1Score > 39 indicates identity
Score > 35 indicates homology		 SSVNSTVLVK + Deamidated (NQ)
464 366.2074 730.4002 730.4086 -11.4 1 27 0.45 +1Score > 45 indicates identity
Score > 36 indicates homology		 NNSIKR
1245 536.7878 1071.5610 1071.5560 4.73 1 27 0.42 +1Score > 42 indicates identity
Score > 36 indicates homology		 NIAQALNEAK + Deamidated (NQ)
1239 535.8445 1069.6744 1069.6648 9.04 0 27 0.17 +1Score > 32 indicates identity LIILPQAFR
1208 350.8747 1049.6023 1049.5869 14.6 1 27 0.33 +1Score > 36 indicates identity
Score > 35 indicates homology		 SPISYTVKR
1684 638.8565 1275.6984 1275.6935 3.85 0 27 0.93 +1Score > 39 indicates identity
Score > 39 indicates homology		 SVLFAAVGTAGQR
495 747.4800 746.4727 746.4691 4.92 0 27 0.2 +1Score > 33 indicates identity FQIVLK
499 374.2440 746.4734 746.4691 5.89 0 27 0.094 +1Score > 32 indicates identity
Score > 29 indicates homology		 AGFVILK
632 801.4992 800.4919 800.4868 6.38 1 27 0.55 +1Score > 37 indicates identity KLAVSQR
582 393.7284 785.4422 785.4395 3.46 1 27 0.76 +1Score > 43 indicates identity
Score > 38 indicates homology		 IGGAEIAR
1313 555.2850 1108.5554 1108.5513 3.74 1 27 0.9 +1Score > 41 indicates identity
Score > 39 indicates homology		 FGGTSVGSIER
224 602.3901 601.3828 601.3799 4.84 0 27 0.34 +1Score > 41 indicates identity
Score > 35 indicates homology		 SVLVGK
631 401.2522 800.4898 800.4868 3.79 1 27 0.64 +1Score > 40 indicates identity
Score > 38 indicates homology		 QSGLLRK
1647 629.8676 1257.7206 1257.7081 9.95 0 27 0.12 +1Score > 37 indicates identity
Score > 30 indicates homology		 LTPFLGVLQDR
863 452.7690 903.5234 903.5178 6.28 1 27 0.12 +1Score > 38 indicates identity
Score > 30 indicates homology		 LEFIINR
401 351.2148 700.4150 700.4119 4.48 1 27 0.81 +1Score > 43 indicates identity
Score > 39 indicates homology		 AIGAIEK
2111 743.4316 1484.8486 1484.8311 11.8 1 27 0.16 +1Score > 36 indicates identity
Score > 32 indicates homology		 IQQVLNTASKLNR + Deamidated (NQ)
1947 702.8453 1403.6760 1403.6892 -9.39 1 27 0.22 +1Score > 41 indicates identity
Score > 33 indicates homology		 VTQSVSEAAALGDR + Deamidated (NQ)
525 380.2228 758.4310 758.4286 3.16 0 27 0.39 +1Score > 44 indicates identity
Score > 35 indicates homology		 AVGAVSAGK
1168 513.7842 1025.5538 1025.5505 3.23 1 27 0.55 +1Score > 38 indicates identity
Score > 37 indicates homology		 HAAELSLTGK
526 380.2309 758.4472 758.4473 -0.024 0 27 0.72 +1Score > 41 indicates identity
Score > 38 indicates homology		 VMKPLR + Oxidation (M)
1579 614.8385 1227.6624 1227.6710 -6.97 1 27 0.75 +1Score > 40 indicates identity
Score > 38 indicates homology		 NILNEAQIIAK + 2 Deamidated (NQ)
537 763.3405 762.3332 762.3297 4.69 1 27 0.82 +1Score > 40 indicates identity
Score > 38 indicates homology		 SADEWR
580 392.7702 783.5258 783.5218 5.16 0 27 0.18 +1Score > 32 indicates identity IIAQIVK
1144 339.5306 1015.5700 1015.5774 -7.35 1 27 0.71 +1Score > 40 indicates identity
Score > 38 indicates homology		 LSTRDVVAR
817 444.2582 886.5018 886.5171 -17.2 1 27 0.42 +1Score > 42 indicates identity
Score > 36 indicates homology		 QMARLLR
1794 663.8263 1325.6380 1325.6398 -1.30 0 27 0.24 +1Score > 41 indicates identity
Score > 33 indicates homology		 QPMSLTHQQQK + Deamidated (NQ)
823 445.7393 889.4640 889.4756 -13.0 1 27 0.54 +1Score > 43 indicates identity
Score > 36 indicates homology		 LSDKLVSE
1042 489.2765 976.5384 976.5229 15.9 1 26 0.51 +1Score > 41 indicates identity
Score > 36 indicates homology		 AFQILAKGE + Deamidated (NQ)
1001 479.7535 957.4924 957.5032 -11.2 0 26 0.29 +1Score > 41 indicates identity
Score > 34 indicates homology		 VIQWQGAR + Deamidated (NQ)
656 408.2363 814.4580 814.4548 3.93 1 26 0.77 +1Score > 43 indicates identity
Score > 38 indicates homology		 EIIIDGR
803 440.7549 879.4952 879.4814 15.7 0 26 0.32 +1Score > 37 indicates identity
Score > 34 indicates homology		 SLFVASTR
824 446.2457 890.4768 890.4610 17.8 0 26 0.43 +1Score > 41 indicates identity
Score > 35 indicates homology		 ALAGFDAAR
1023 483.7742 965.5338 965.5294 4.60 0 26 0.77 +1Score > 38 indicates identity LQQHQLAK + Deamidated (NQ)
267 315.7022 629.3898 629.3861 6.01 0 26 0.25 +1Score > 41 indicates identity
Score > 33 indicates homology		 VGLSVR
2330 790.8916 1579.7686 1579.7730 -2.74 0 26 0.063 +1Score > 41 indicates identity
Score > 27 indicates homology		 YTVQNLTVQAVDTE
234 306.6964 611.3782 611.3755 4.51 0 26 0.42 +1Score > 35 indicates identity AVAVPR
1098 502.2819 1002.5492 1002.5346 14.6 0 26 0.48 +1Score > 41 indicates identity
Score > 35 indicates homology		 VTNVAGINSK + Deamidated (NQ)
468 366.7051 731.3956 731.3926 4.16 0 26 0.65 +1Score > 44 indicates identity
Score > 37 indicates homology		 TGTGQIR
225 603.3383 602.3310 602.3275 5.80 1 26 0.96 +1Score > 46 indicates identity
Score > 38 indicates homology		 EIDVK
545 383.6916 765.3686 765.3657 3.84 1 26 0.2 +1Score > 42 indicates identity
Score > 32 indicates homology		 VESFQR + Deamidated (NQ)
933 465.7692 929.5238 929.5083 16.8 1 26 0.16 +1Score > 42 indicates identity
Score > 31 indicates homology		 LGAAWQKR + Deamidated (NQ)
1074 497.7726 993.5306 993.5356 -4.94 0 26 0.26 +1Score > 40 indicates identity
Score > 33 indicates homology		 NHIDNLLR
345 673.3979 672.3906 672.3806 14.9 0 26 0.93 +1Score > 43 indicates identity
Score > 38 indicates homology		 AGGDILK
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