MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3490 746.3831 2236.1275 2236.1535 -11.6 1 8 0.93 +1Score > 39 indicates identity
Score > 20 indicates homology
IHQDGIHILVNMNGYTKGAR
173 531.2930 530.2857 530.2813 8.44 1 8 0.78 +1Score > 46 indicates identity
Score > 20 indicates homology
ARGDI
667 412.2328 822.4510 822.4599 -10.8 1 8 0.48 +1Score > 39 indicates identity
Score > 17 indicates homology
YGQKSIK
2739 886.4586 1770.9026 1770.9325 -16.8 0 8 0.89 +1Score > 40 indicates identity
Score > 20 indicates homology
ILDLQAPIMSLQSVLE + 2 Deamidated (NQ)
2963 964.0328 1926.0510 1926.0397 5.90 1 8 0.71 +1Score > 36 indicates identity
Score > 19 indicates homology
VIQPIQSIFRVSHCIK + 2 Deamidated (NQ)
1788 662.3713 1322.7280 1322.7194 6.53 0 8 0.66 +1Score > 38 indicates identity
Score > 19 indicates homology
TPVILIDPQGNR + Deamidated (NQ)
2181 759.9182 1517.8218 1517.8487 -17.7 1 8 0.86 +1Score > 39 indicates identity
Score > 20 indicates homology
ILNKQSGMLGISLK + Deamidated (NQ); Oxidation (M)
3198 691.4093 2071.2061 2071.1711 16.9 1 8 0.43 +1Score > 31 indicates identity
Score > 17 indicates homology
LTVIKNLVDSAQAMVLALR + Deamidated (NQ); Oxidation (M)
1720 647.3420 1292.6694 1292.6646 3.77 0 8 0.9 +1Score > 41 indicates identity
Score > 20 indicates homology
TALAMGLSQSLGK + Deamidated (NQ); Oxidation (M)
3326 715.3663 2143.0771 2143.0949 -8.33 1 8 0.66 +1Score > 39 indicates identity
Score > 19 indicates homology
TFPPTAAQLQQLKQLHYR + 4 Deamidated (NQ)
2498 833.4631 1664.9116 1664.8821 17.8 1 8 0.7 +1Score > 37 indicates identity
Score > 19 indicates homology
GKAPIGRPSPMSPWGK
2513 834.4456 1666.8766 1666.8460 18.4 1 8 0.78 +1Score > 39 indicates identity
Score > 20 indicates homology
HNARALVNAGAAIMIE + Deamidated (NQ); Oxidation (M)
2917 473.0092 1888.0077 1888.0306 -12.1 1 8 0.65 +1Score > 38 indicates identity
Score > 19 indicates homology
VQFVQNITDIDDKIIK
3363 541.0254 2160.0725 2160.0919 -8.98 1 8 0.82 +1Score > 39 indicates identity
Score > 20 indicates homology
ELDVQDVNVAITMCVLGLR + Oxidation (M)
467 732.3935 731.3862 731.3926 -8.69 1 8 0.94 +1Score > 43 indicates identity
Score > 20 indicates homology
KGGLSNR + Deamidated (NQ)
3841 622.8292 2487.2877 2487.2719 6.33 1 8 0.31 +1Score > 37 indicates identity
Score > 16 indicates homology
TALPAWVEFMSLALQDVPVQQK + Deamidated (NQ); Oxidation (M)
2463 546.9953 1637.9641 1637.9716 -4.60 0 8 0.65 +1Score > 31 indicates identity
Score > 19 indicates homology
LLQLNGSLVVVGIVGR + 2 Deamidated (NQ)
2805 906.4395 1810.8644 1810.8771 -6.98 1 8 0.36 +1Score > 40 indicates identity
Score > 16 indicates homology
QGLEAMGAGLASVTYQAK + Deamidated (NQ); Oxidation (M)
1505 598.8383 1195.6620 1195.6601 1.65 1 8 0.84 +1Score > 38 indicates identity
Score > 20 indicates homology
KGIPYAYVASK
3900 851.1255 2550.3547 2550.3905 -14.0 1 8 0.2 +1Score > 35 indicates identity
Score > 14 indicates homology
NASVPQILIIVTDGKSQGDVALPSK + Deamidated (NQ)
3698 1181.1156 2360.2166 2360.1722 18.8 1 8 0.53 +1Score > 38 indicates identity
Score > 18 indicates homology
FIMQINDDIPQRASLPQPFK + 3 Deamidated (NQ)
2390 805.9738 1609.9330 1609.9291 2.48 1 8 0.4 +1Score > 34 indicates identity
Score > 17 indicates homology
SLVPLEDLQGVLSLK
3695 787.4094 2359.2064 2359.1689 15.9 1 8 0.29 +1Score > 38 indicates identity
Score > 15 indicates homology
TLGQQVLVENMGGAGGSLGAANVAK + 2 Deamidated (NQ); Oxidation (M)
3384 1087.0425 2172.0704 2172.0409 13.6 1 8 0.83 +1Score > 39 indicates identity
Score > 20 indicates homology
NTMYAQVFNLQGKTPVSQK + 3 Deamidated (NQ); Oxidation (M)
648 809.4374 808.4301 808.4304 -0.31 0 8 0.28 +1Score > 40 indicates identity
Score > 15 indicates homology
QLAAGGHR
2209 765.4330 1528.8514 1528.8759 -16.0 1 8 0.39 +1Score > 37 indicates identity
Score > 16 indicates homology
IVQTLCTVRGAALK
228 304.1628 606.3110 606.3159 -8.06 0 8 0.57 +1Score > 43 indicates identity
Score > 18 indicates homology
MVTTR
1417 578.3037 1154.5928 1154.6084 -13.5 0 8 0.49 +1Score > 40 indicates identity
Score > 17 indicates homology
GNIYVYASLR
2978 644.7041 1931.0905 1931.0887 0.93 1 8 1 +1Score > 32 indicates identity
Score > 20 indicates homology
MINIVNNKVNIIHRPR + Deamidated (NQ)
1950 351.9377 1403.7217 1403.7078 9.87 1 8 0.97 +1Score > 41 indicates identity
Score > 20 indicates homology
ARDAAQMVADLVK + Deamidated (NQ); Oxidation (M)
475 368.2251 734.4356 734.4439 -11.2 1 8 0.85 +1Score > 36 indicates identity
Score > 20 indicates homology
LTFKAR
2430 813.8971 1625.7796 1625.7896 -6.15 1 8 0.78 +1Score > 40 indicates identity
Score > 19 indicates homology
TVGPNSQEALLPQNR + 3 Deamidated (NQ)
2699 584.9802 1751.9188 1751.9319 -7.47 0 8 0.56 +1Score > 39 indicates identity
Score > 18 indicates homology
LQYPQDAVLALTQHR
2712 586.9850 1757.9332 1757.9273 3.34 1 8 0.72 +1Score > 39 indicates identity
Score > 19 indicates homology
YKQISNLLVLYMQK + 2 Deamidated (NQ); Oxidation (M)
2807 907.4951 1812.9756 1812.9842 -4.70 1 8 0.89 +1Score > 38 indicates identity
Score > 20 indicates homology
IVVCITEGIPVLDMVR
3037 660.3278 1977.9616 1977.9288 16.6 1 8 0.48 +1Score > 40 indicates identity
Score > 17 indicates homology
MSAIFSFQSLARCSQTK + Deamidated (NQ); Oxidation (M)
3322 714.0527 2139.1363 2139.1423 -2.82 1 8 0.66 +1Score > 37 indicates identity
Score > 19 indicates homology
IVIATDSVIQPARALVTDQE + Deamidated (NQ)
4402 1015.4973 3043.4701 3043.4525 5.77 1 8 1 +1Score > 37 indicates identity
Score > 20 indicates homology
LVLMGSAGVSFPITEGLDAVWGYNPSFAE + Deamidated (NQ); Oxidation (M)
518 379.2155 756.4164 756.4130 4.57 0 8 0.56 +1Score > 39 indicates identity
Score > 18 indicates homology
ANTVVPR + Deamidated (NQ)
947 313.1796 936.5170 936.5141 3.08 1 8 0.76 +1Score > 38 indicates identity
Score > 19 indicates homology
AHSAPKAQK
2253 775.9039 1549.7932 1549.8174 -15.6 1 8 0.77 +1Score > 41 indicates identity
Score > 19 indicates homology
VVLSFAAEMLVQAR + Deamidated (NQ); Oxidation (M)
3660 584.7867 2335.1177 2335.1339 -6.94 1 8 0.36 +1Score > 39 indicates identity
Score > 16 indicates homology
NASKGPHSPQVPSQVQSPMTSR + Oxidation (M)
3881 842.4584 2524.3534 2524.3286 9.83 1 8 0.29 +1Score > 35 indicates identity
Score > 15 indicates homology
DTVVVDLLGLNKGVAWVNGNNLGR + 2 Deamidated (NQ)
1302 552.8279 1103.6412 1103.6260 13.8 1 8 0.36 +1Score > 37 indicates identity
Score > 16 indicates homology
KVVLLGMSLE + Oxidation (M)
2949 959.0070 1915.9994 1916.0268 -14.3 1 8 0.85 +1Score > 39 indicates identity
Score > 20 indicates homology
IDAVRAAFNAVGQLSWAK
3002 649.7169 1946.1289 1946.1029 13.3 0 8 0.79 +1Score > 30 indicates identity
Score > 19 indicates homology
LLGNPWGFFLSSLIIIR + Deamidated (NQ)
3611 769.7083 2306.1031 2306.1464 -18.8 1 8 0.58 +1Score > 39 indicates identity
Score > 18 indicates homology
FQSDLKIINDCLDGLIQNAK + 2 Deamidated (NQ)
3891 844.7614 2531.2624 2531.2220 15.9 0 8 0.36 +1Score > 38 indicates identity
Score > 16 indicates homology
FDEPWTLLQNQNGLLTWLVDE + Deamidated (NQ)
2981 967.5001 1932.9856 1933.0165 -16.0 1 8 0.73 +1Score > 39 indicates identity
Score > 19 indicates homology
YNITILMITHEMSVIR
4184 701.8635 2803.4249 2803.4062 6.67 1 8 0.31 +1Score > 37 indicates identity
Score > 15 indicates homology
GNDVIANDLENLAASFQAAVVDVLMAK + Oxidation (M)
4203 939.1823 2814.5251 2814.5396 -5.17 1 8 0.24 +1Score > 32 indicates identity
Score > 14 indicates homology
MRTLGAMAIMLVVMGTVIFLSFILR + 2 Oxidation (M)
3746 801.1005 2400.2797 2400.2511 11.9 1 8 0.66 +1Score > 36 indicates identity
Score > 19 indicates homology
AEIAGHTVWLLKPATFMNLSGK + Deamidated (NQ); Oxidation (M)
2979 644.7046 1931.0920 1931.0887 1.71 1 8 1 +1Score > 32 indicates identity
Score > 20 indicates homology
MINIVNNKVNIIHRPR + Deamidated (NQ)
3203 692.7155 2075.1247 2075.1296 -2.39 1 8 0.38 +1Score > 36 indicates identity
Score > 16 indicates homology
SVVINTLCQAQTKLGLTTK + Deamidated (NQ)
4333 737.3911 2945.5353 2945.4917 14.8 1 8 0.41 +1Score > 35 indicates identity
Score > 17 indicates homology
DVRNCSTSPPYLPVTAVNTTAQLTALR + Deamidated (NQ)
1891 690.3713 1378.7280 1378.7014 19.4 0 8 0.77 +1Score > 39 indicates identity
Score > 19 indicates homology
NISLMTLDSLQK + Deamidated (NQ); Oxidation (M)
2579 851.4611 1700.9076 1700.8985 5.40 1 8 0.86 +1Score > 39 indicates identity
Score > 20 indicates homology
STLQPENSVPIKPYK + Deamidated (NQ)
3083 668.3340 2001.9802 2001.9948 -7.32 1 8 0.33 +1Score > 40 indicates identity
Score > 16 indicates homology
NPHYRYPITQNLFPLE + Deamidated (NQ)
3144 680.3531 2038.0375 2038.0273 5.01 1 8 0.34 +1Score > 39 indicates identity
Score > 16 indicates homology
RHGSVVGIPYWDTVVPQE
1548 405.8962 1214.6668 1214.6619 4.03 1 8 0.8 +1Score > 40 indicates identity
Score > 19 indicates homology
DGTNLLQGLKR + Deamidated (NQ)
3231 700.0079 2097.0019 2097.0387 -17.6 0 8 0.61 +1Score > 39 indicates identity
Score > 18 indicates homology
NFDFRPGMIIINLDLMR + Deamidated (NQ); 2 Oxidation (M)
4451 1038.8264 3113.4574 3113.5014 -14.1 1 8 0.42 +1Score > 36 indicates identity
Score > 17 indicates homology
KNLPNNAGDLGLGAGAATPGSSQDFTVNVGDR + Deamidated (NQ)
1925 465.8990 1394.6752 1394.6929 -12.7 0 8 0.46 +1Score > 41 indicates identity
Score > 17 indicates homology
NIVPILADANQPE + 2 Deamidated (NQ)
358 338.2079 674.4012 674.3897 17.1 1 8 0.88 +1Score > 39 indicates identity
Score > 20 indicates homology
AAMKVR
2444 816.4521 1630.8896 1630.8719 10.9 0 8 0.97 +1Score > 38 indicates identity
Score > 20 indicates homology
LTLHGLQQYFVALE
3036 495.2803 1977.0921 1977.0968 -2.40 0 8 0.33 +1Score > 35 indicates identity
Score > 15 indicates homology
EPLLLLLSPAAATVGEPMR
3038 660.3581 1978.0525 1978.0234 14.7 0 8 0.68 +1Score > 37 indicates identity
Score > 19 indicates homology
IGYFGQPLTDAPGALALMK + Oxidation (M)
3126 679.0244 2034.0514 2034.0390 6.06 1 8 0.68 +1Score > 39 indicates identity
Score > 19 indicates homology
MFDRILLDAPCSATGVIR
2469 548.2958 1641.8656 1641.8475 11.0 1 8 0.6 +1Score > 40 indicates identity
Score > 18 indicates homology
NARNGHLFVTDGIVK + 2 Deamidated (NQ)
2937 637.3323 1908.9751 1908.9707 2.29 1 8 0.24 +1Score > 39 indicates identity
Score > 14 indicates homology
FYNQHLINHVGRGTPR + Deamidated (NQ)
2787 601.9862 1802.9368 1802.9567 -11.0 0 8 0.75 +1Score > 39 indicates identity
Score > 19 indicates homology
LGGQWPPTGVKPLNPLE + Deamidated (NQ)
2792 603.3420 1807.0042 1807.0131 -4.96 1 8 0.98 +1Score > 35 indicates identity
Score > 20 indicates homology
LFLGVVFNKLDISVDK + Deamidated (NQ)
2419 808.4551 1614.8956 1614.8941 0.98 1 8 0.65 +1Score > 37 indicates identity
Score > 18 indicates homology
VLQTLGPTRSSSQLK + Deamidated (NQ)
3738 798.0889 2391.2449 2391.2124 13.6 1 8 0.39 +1Score > 37 indicates identity
Score > 16 indicates homology
TVAFQWPAGFYSDGIHIGLRR + Deamidated (NQ)
3597 767.4147 2299.2223 2299.2093 5.64 0 8 0.71 +1Score > 37 indicates identity
Score > 19 indicates homology
AIIALLTLMQDNGITADAVAGAR + 2 Deamidated (NQ)
4601 1134.6208 3400.8406 3400.8655 -7.33 0 8 1 +1Score > 31 indicates identity
Score > 20 indicates homology
MIEPLLCGIVLGLVPITLLGLFVSAWNQYR + Oxidation (M)
2735 590.9619 1769.8639 1769.8910 -15.3 1 8 0.68 +1Score > 40 indicates identity
Score > 19 indicates homology
MVTLLNFDPSFKLTE + Oxidation (M)
3223 524.5314 2094.0965 2094.0997 -1.54 1 8 0.49 +1Score > 38 indicates identity
Score > 17 indicates homology
EGVIAVYDLGGGTFDISILR
3636 773.7633 2318.2681 2318.3097 -17.9 1 8 1 +1Score > 34 indicates identity
Score > 20 indicates homology
SPNPQLLTIPEAATILLASLQK + Deamidated (NQ)
2413 538.9841 1613.9305 1613.9141 10.1 0 8 0.64 +1Score > 34 indicates identity
Score > 18 indicates homology
LIGQQGLVDGVFLVR + Deamidated (NQ)
2892 469.2702 1873.0517 1873.0343 9.31 1 8 0.91 +1Score > 34 indicates identity
Score > 20 indicates homology
MAKLQQLVQAIQSGTIK + Deamidated (NQ); Oxidation (M)
4677 908.4236 3629.6653 3629.6952 -8.25 1 8 1 +1Score > 33 indicates identity
Score > 20 indicates homology
FSHMTAIAPNATSSIIMGNTSPSCEPFRANIYK + Deamidated (NQ); Oxidation (M)
2026 722.3765 1442.7384 1442.7187 13.7 1 8 0.51 +1Score > 41 indicates identity
Score > 17 indicates homology
LQQLKDMSSHQK + Deamidated (NQ)
2076 735.3745 1468.7344 1468.7522 -12.1 0 8 0.88 +1Score > 40 indicates identity
Score > 20 indicates homology
DVVNIGIGGSDLGPR + Deamidated (NQ)
1169 343.1927 1026.5563 1026.5386 17.2 0 8 0.66 +1Score > 39 indicates identity
Score > 18 indicates homology
LGFTDLFSK
3018 983.4687 1964.9228 1964.9327 -5.01 1 8 0.96 +1Score > 39 indicates identity
Score > 20 indicates homology
KSQVFSTADDNQPAVSIR + 3 Deamidated (NQ)
2495 554.2904 1659.8494 1659.8614 -7.23 1 8 0.98 +1Score > 40 indicates identity
Score > 20 indicates homology
GGQLVRAAGTAAQLMAK + 2 Deamidated (NQ); Oxidation (M)
2761 895.4332 1788.8518 1788.8433 4.78 0 8 0.69 +1Score > 40 indicates identity
Score > 18 indicates homology
TYTMFFIANYFLDK + Oxidation (M)
2147 752.4165 1502.8184 1502.8093 6.09 1 8 0.87 +1Score > 38 indicates identity
Score > 20 indicates homology
ILGQVFIRDVQSE
1640 418.8836 1253.6290 1253.6040 19.9 0 8 0.95 +1Score > 40 indicates identity
Score > 20 indicates homology
VYSVAFSPDNR
2116 497.9286 1490.7640 1490.7828 -12.6 1 8 0.98 +1Score > 41 indicates identity
Score > 20 indicates homology
QNTLATLTKLASTE + Deamidated (NQ)
3421 730.3746 2188.1020 2188.1198 -8.14 1 8 0.45 +1Score > 39 indicates identity
Score > 17 indicates homology
INAAHGFSLIQVDNTKVTMK + 2 Deamidated (NQ)
3562 1138.0193 2274.0240 2274.0521 -12.3 1 8 0.83 +1Score > 37 indicates identity
Score > 19 indicates homology
QMSQQMHQAAADLQFELAAR + Deamidated (NQ)
876 455.7910 909.5674 909.5508 18.3 0 8 0.97 +1Score > 32 indicates identity
Score > 20 indicates homology
AALQRPVR
3107 674.0095 2019.0067 2019.0421 -17.5 0 8 0.42 +1Score > 40 indicates identity
Score > 16 indicates homology
TVQITIMSLIAMTVYFR + Deamidated (NQ); 2 Oxidation (M)
2671 871.9614 1741.9082 1741.8934 8.55 1 8 0.88 +1Score > 39 indicates identity
Score > 20 indicates homology
SIPNIMDGWKPGQRK + Oxidation (M)
3918 852.4501 2554.3285 2554.3504 -8.57 1 8 0.26 +1Score > 36 indicates identity
Score > 14 indicates homology
SGRAIASHQSVLIFDVLSTSDVPR
1879 685.8866 1369.7586 1369.7752 -12.1 0 8 0.94 +1Score > 39 indicates identity
Score > 20 indicates homology
LVGGVVQMIPVSR + Oxidation (M)
2203 509.9659 1526.8759 1526.8854 -6.26 1 8 0.66 +1Score > 35 indicates identity
Score > 18 indicates homology
VGLNPVKINTVVMK + Oxidation (M)
2705 586.3842 1756.1308 1756.0974 19.0 1 8 0.45 +1Score > 17 indicates identity IPIVIVANKADLLHLK
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