MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2778 601.6097 1801.8073 1801.8329 -14.2 1 9 0.48 +1Score > 38 indicates identity
Score > 18 indicates homology
QALNGNDNLANAKQQAK + 5 Deamidated (NQ)
3060 663.6814 1988.0224 1988.0214 0.48 1 9 0.88 +1Score > 39 indicates identity
Score > 21 indicates homology
NIAQALFNQDKINDLLR + 3 Deamidated (NQ)
3101 1009.5160 2017.0174 2016.9898 13.7 1 9 0.71 +1Score > 39 indicates identity
Score > 20 indicates homology
SLNQQRLQGQLCDVSIR + 3 Deamidated (NQ)
3546 756.0570 2265.1492 2265.1511 -0.83 1 9 0.59 +1Score > 39 indicates identity
Score > 19 indicates homology
RLWVAPSCSLLHSPVDLCR
3714 594.0930 2372.3429 2372.3315 4.80 1 9 0.37 +1Score > 30 indicates identity
Score > 17 indicates homology
VPDTLSPQVPVIEAVLDALGIAR
1992 713.8412 1425.6678 1425.6744 -4.62 0 9 0.72 +1Score > 40 indicates identity
Score > 20 indicates homology
HQTALIMPICNE
3127 1018.0379 2034.0612 2034.0382 11.3 1 9 0.71 +1Score > 38 indicates identity
Score > 20 indicates homology
NGSDDAVLAYKLLVQTGNR + Deamidated (NQ)
1742 435.2610 1302.7612 1302.7442 13.0 1 9 0.29 +1Score > 34 indicates identity
Score > 16 indicates homology
LVRSTTVLCVR
3252 1057.5896 2113.1646 2113.1531 5.45 1 9 0.45 +1Score > 34 indicates identity
Score > 18 indicates homology
LVRQLQGDNIVYLNNLLE
3851 624.5496 2494.1693 2494.2009 -12.7 1 9 0.26 +1Score > 38 indicates identity
Score > 15 indicates homology
HQAISMVPSEQEPNTVVNVLQR + 3 Deamidated (NQ); Oxidation (M)
1588 410.8687 1229.5843 1229.5751 7.49 1 9 0.52 +1Score > 40 indicates identity
Score > 19 indicates homology
SDPPLCWVEK
2420 539.5975 1615.7707 1615.7916 -12.9 1 9 0.6 +1Score > 40 indicates identity
Score > 19 indicates homology
SIFYVSLEDQLMR + Oxidation (M)
2898 940.0020 1877.9894 1878.0098 -10.9 1 9 0.76 +1Score > 38 indicates identity
Score > 20 indicates homology
VTEFASLLSNAGIVTTVR + Deamidated (NQ)
218 602.3637 601.3564 601.3548 2.78 0 9 0.86 +1Score > 43 indicates identity
Score > 21 indicates homology
AGVSIR
1342 562.8083 1123.6020 1123.5986 3.09 0 9 0.96 +1Score > 39 indicates identity
Score > 21 indicates homology
SGVGAAHIIDGK
833 448.2081 894.4016 894.4117 -11.2 0 9 0.48 +1Score > 41 indicates identity
Score > 18 indicates homology
MTNADLSK + Oxidation (M)
988 478.7691 955.5236 955.5239 -0.31 0 9 0.87 +1Score > 39 indicates identity
Score > 21 indicates homology
LFSLPHSR
1465 391.5695 1171.6867 1171.6673 16.5 1 9 0.71 +1Score > 37 indicates identity
Score > 20 indicates homology
ANTGGLSKLVGR
2546 558.9855 1673.9347 1673.9134 12.7 1 9 0.28 +1Score > 36 indicates identity
Score > 16 indicates homology
VSLQNLSMVLRQLR + 2 Deamidated (NQ); Oxidation (M)
2128 748.4555 1494.8964 1494.8770 13.0 0 9 0.21 +1Score > 30 indicates identity
Score > 15 indicates homology
LAADVTGVPTLLVAR
1818 446.8758 1337.6056 1337.6133 -5.75 1 9 0.76 +1Score > 40 indicates identity
Score > 20 indicates homology
VMSENTVNSALR + 2 Deamidated (NQ); Oxidation (M)
3345 718.3825 2152.1257 2152.1310 -2.47 1 9 0.45 +1Score > 38 indicates identity
Score > 18 indicates homology
QALNSLAQMEPALVRQLPR + 2 Deamidated (NQ); Oxidation (M)
3362 541.0253 2160.0721 2160.0997 -12.8 1 9 0.55 +1Score > 39 indicates identity
Score > 19 indicates homology
GISDLAQHYLMRANITAIR + 2 Deamidated (NQ); Oxidation (M)
3593 1150.0906 2298.1666 2298.1300 15.9 1 9 0.91 +1Score > 38 indicates identity
Score > 21 indicates homology
NPENLINDAPIKPTLIGMSNK + 4 Deamidated (NQ); Oxidation (M)
3230 525.2567 2096.9977 2097.0122 -6.92 0 9 0.56 +1Score > 39 indicates identity
Score > 19 indicates homology
AMLDSLNIPYVIDTNMVR + Deamidated (NQ); 2 Oxidation (M)
2193 381.4389 1521.7265 1521.7497 -15.3 1 9 0.71 +1Score > 41 indicates identity
Score > 20 indicates homology
VVEAGYADPVSLMR + Oxidation (M)
2853 617.2756 1848.8050 1848.7869 9.75 1 9 0.2 +1Score > 37 indicates identity
Score > 14 indicates homology
KMSGHMGAVQQSTQNLE + 4 Deamidated (NQ)
3000 972.5471 1943.0796 1943.0411 19.8 1 9 0.3 +1Score > 34 indicates identity
Score > 16 indicates homology
RNVLVMSFLGDQGLAAPR
3194 691.0284 2070.0634 2070.0415 10.5 1 9 0.7 +1Score > 39 indicates identity
Score > 20 indicates homology
VTKANVVNMHDDIVNVSSK + Deamidated (NQ)
3527 751.3667 2251.0783 2251.0467 14.0 1 9 0.23 +1Score > 39 indicates identity
Score > 15 indicates homology
YAVLSDILGNEDHLGDMDFK
3649 776.0327 2325.0763 2325.0956 -8.30 1 9 0.96 +1Score > 38 indicates identity
Score > 21 indicates homology
SILTMMMGVYRDFNITDYR
1814 668.8308 1335.6470 1335.6531 -4.55 1 9 0.91 +1Score > 41 indicates identity
Score > 21 indicates homology
TDQYRQVSSPR
957 941.4896 940.4823 940.4978 -16.4 1 9 0.86 +1Score > 39 indicates identity
Score > 21 indicates homology
LALENPQR + Deamidated (NQ)
1274 1088.5781 1087.5708 1087.5734 -2.39 1 9 0.4 +1Score > 42 indicates identity
Score > 17 indicates homology
GGRSLGSTTPR
2710 879.9727 1757.9308 1757.9022 16.3 1 9 0.47 +1Score > 39 indicates identity
Score > 18 indicates homology
SQFMIRYQIINLSK + 2 Deamidated (NQ); Oxidation (M)
2318 315.9950 1574.9386 1574.9369 1.12 1 9 0.83 +1Score > 29 indicates identity
Score > 20 indicates homology
LVQQRPALPGLARR + Deamidated (NQ)
3553 756.3888 2266.1446 2266.1668 -9.79 0 9 0.41 +1Score > 39 indicates identity
Score > 17 indicates homology
LFQIGVAPSSLTLVAVTHMHE + Deamidated (NQ); Oxidation (M)
3443 1100.0872 2198.1598 2198.1214 17.5 1 9 0.48 +1Score > 37 indicates identity
Score > 18 indicates homology
MLGADQPVILQMLEITPALE + Deamidated (NQ); Oxidation (M)
1061 496.7818 991.5490 991.5338 15.3 1 9 0.66 +1Score > 39 indicates identity
Score > 19 indicates homology
TVYKKPQE
2524 557.3331 1668.9775 1668.9774 0.061 1 9 0.83 +1Score > 32 indicates identity
Score > 20 indicates homology
ISVINSLLDNIKAAAK
1792 442.5793 1324.7161 1324.7350 -14.3 1 9 0.34 +1Score > 38 indicates identity
Score > 16 indicates homology
AQPLDILNKANK + Deamidated (NQ)
2554 562.2955 1683.8647 1683.8791 -8.59 1 9 0.87 +1Score > 40 indicates identity
Score > 21 indicates homology
KALTPVQALSGGQQQR + 3 Deamidated (NQ)
2467 821.4220 1640.8294 1640.8006 17.6 1 9 0.5 +1Score > 40 indicates identity
Score > 18 indicates homology
AVDNGQLQVETVNPR + 2 Deamidated (NQ)
3700 787.7569 2360.2489 2360.2416 3.08 0 9 0.41 +1Score > 36 indicates identity
Score > 17 indicates homology
QTLLAWLDALGWPAPEPGALLE
4216 943.8690 2828.5852 2828.5337 18.2 1 9 0.26 +1Score > 29 indicates identity
Score > 15 indicates homology
DVLLLVHNLPQNLTGHIWYKGQIR + 2 Deamidated (NQ)
1860 455.2507 1362.7303 1362.7368 -4.76 1 9 0.61 +1Score > 40 indicates identity
Score > 19 indicates homology
VALRHTNAINPR + 2 Deamidated (NQ)
2801 906.0010 1809.9874 1809.9633 13.3 0 9 0.68 +1Score > 37 indicates identity
Score > 19 indicates homology
TQIPLMLCWALSIHK
3234 1051.0083 2100.0020 2099.9973 2.28 1 9 0.89 +1Score > 39 indicates identity
Score > 21 indicates homology
LPDPPQPSLLPPDPDMLEE + Deamidated (NQ)
3822 824.4334 2470.2784 2470.2349 17.6 1 9 0.69 +1Score > 37 indicates identity
Score > 19 indicates homology
ITSRYTDLQPVGMGAFGLVCSAK
1995 714.3959 1426.7772 1426.7537 16.5 1 9 0.79 +1Score > 39 indicates identity
Score > 20 indicates homology
HMANNIIAMLKR + Oxidation (M)
3061 663.6968 1988.0686 1988.0289 20.0 1 9 0.18 +1Score > 37 indicates identity
Score > 14 indicates homology
QGPMTPLPDVVIIHTPKE + Deamidated (NQ); Oxidation (M)
3743 800.0866 2397.2380 2397.2210 7.10 0 9 0.34 +1Score > 37 indicates identity
Score > 16 indicates homology
AQLMQQVQLGVDALANSTGQPVK + 2 Deamidated (NQ)
2309 787.9268 1573.8390 1573.8464 -4.66 1 9 0.85 +1Score > 39 indicates identity
Score > 20 indicates homology
YLNVSPEVLNASIR
3677 1175.5817 2349.1488 2349.1488 0.012 1 9 0.99 +1Score > 39 indicates identity
Score > 21 indicates homology
HVYIVSQNFVNNQLVISSER + 4 Deamidated (NQ)
3220 1046.5182 2091.0218 2091.0129 4.30 1 9 0.79 +1Score > 40 indicates identity
Score > 20 indicates homology
LPQPMQLPHGLSENAMLAE + Oxidation (M)
1157 511.7835 1021.5524 1021.5332 18.9 0 9 0.72 +1Score > 39 indicates identity
Score > 20 indicates homology
DTTFALLLE
3652 776.0929 2325.2569 2325.2250 13.7 1 9 0.38 +1Score > 35 indicates identity
Score > 17 indicates homology
TQTDAALLLPQMIAEALGVTPR + Deamidated (NQ); Oxidation (M)
1506 599.3030 1196.5914 1196.5826 7.41 0 9 0.98 +1Score > 40 indicates identity
Score > 21 indicates homology
DLFGLTNNFR + Deamidated (NQ)
2500 833.4651 1664.9156 1664.9097 3.59 1 9 0.8 +1Score > 37 indicates identity
Score > 20 indicates homology
KSEPNPINAIINNLK + Deamidated (NQ)
2198 763.8698 1525.7250 1525.7446 -12.8 0 8 0.89 +1Score > 40 indicates identity
Score > 21 indicates homology
SHMSPNTIIAPITE + Oxidation (M)
3452 1103.6416 2205.2686 2205.2773 -3.90 1 8 0.83 +1Score > 29 indicates identity
Score > 20 indicates homology
NVIDQALIKPAKYVPITPPK + Deamidated (NQ)
3623 770.7757 2309.3053 2309.2995 2.51 1 8 0.49 +1Score > 31 indicates identity
Score > 18 indicates homology
FGPIIQTGDGLLLLKQVQPSGK + Deamidated (NQ)
1441 583.3296 1164.6446 1164.6615 -14.5 1 8 0.85 +1Score > 38 indicates identity
Score > 20 indicates homology
ARHILVTINE
2516 834.4549 1666.8952 1666.9076 -7.41 1 8 0.17 +1Score > 38 indicates identity
Score > 13 indicates homology
LAMLQLTHNKLLQE + Oxidation (M)
165 516.3160 515.3087 515.3067 3.87 1 8 0.79 +1Score > 47 indicates identity
Score > 20 indicates homology
KGSPK
1903 347.4503 1385.7721 1385.7779 -4.21 1 8 0.59 +1Score > 38 indicates identity
Score > 19 indicates homology
LPQVRVLDYQR
1963 472.2664 1413.7774 1413.7715 4.15 0 8 0.95 +1Score > 39 indicates identity
Score > 21 indicates homology
GSDLLVNIDIDLK
3030 986.5336 1971.0526 1971.0248 14.1 1 8 0.96 +1Score > 38 indicates identity
Score > 21 indicates homology
IMAVVKANGYGHGLLNVAE + Oxidation (M)
2084 737.8723 1473.7300 1473.7061 16.2 0 8 0.51 +1Score > 41 indicates identity
Score > 18 indicates homology
VSPYDPLDLQMVP + Deamidated (NQ)
2854 925.4539 1848.8932 1848.9026 -5.08 1 8 0.78 +1Score > 40 indicates identity
Score > 20 indicates homology
GQLSSVTAVLQEMNLLE + 2 Deamidated (NQ); Oxidation (M)
1372 380.2009 1137.5809 1137.5666 12.6 1 8 0.87 +1Score > 41 indicates identity
Score > 20 indicates homology
KDHAVQPLTE + Deamidated (NQ)
3351 1078.1045 2154.1944 2154.2194 -11.6 1 8 0.54 +1Score > 33 indicates identity
Score > 18 indicates homology
RVSNLVPMTIIGAASNLIIR + Deamidated (NQ); Oxidation (M)
3001 649.7075 1946.1007 1946.0625 19.6 1 8 0.36 +1Score > 33 indicates identity
Score > 16 indicates homology
IGPVSDVLRQLFALFDR + Deamidated (NQ)
3512 749.0549 2244.1429 2244.1386 1.90 0 8 0.39 +1Score > 39 indicates identity
Score > 17 indicates homology
AQPAGPTAQPGGTAPGLAALQALGE + Deamidated (NQ)
1863 456.2160 1365.6262 1365.6466 -15.0 1 8 0.55 +1Score > 40 indicates identity
Score > 18 indicates homology
WPFDKFQGADR
2099 739.3539 1476.6932 1476.7018 -5.76 1 8 0.72 +1Score > 40 indicates identity
Score > 20 indicates homology
QMTLVLEAQQLGE + 2 Deamidated (NQ); Oxidation (M)
2602 855.4191 1708.8236 1708.8301 -3.80 1 8 0.62 +1Score > 41 indicates identity
Score > 19 indicates homology
QMSQQNLTKQTGLSK + 2 Deamidated (NQ); Oxidation (M)
3233 1050.5619 2099.1092 2099.0899 9.24 1 8 0.76 +1Score > 37 indicates identity
Score > 20 indicates homology
VLSLLNDVGSGIQYLHENK + Deamidated (NQ)
2696 876.4315 1750.8484 1750.8573 -5.06 1 8 0.91 +1Score > 41 indicates identity
Score > 20 indicates homology
ARFHDAMNILGPQHK + Deamidated (NQ); Oxidation (M)
3439 733.3659 2197.0759 2197.0434 14.8 0 8 0.86 +1Score > 39 indicates identity
Score > 20 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
2425 810.4219 1618.8292 1618.8031 16.1 1 8 0.36 +1Score > 40 indicates identity
Score > 16 indicates homology
KNSFFFPYGVNIGK + 2 Deamidated (NQ)
4378 1004.8800 3011.6182 3011.5750 14.3 1 8 1 +1Score > 33 indicates identity
Score > 21 indicates homology
LLNQGGLFVLTGASAALNRTSGMIAYGATK + Deamidated (NQ); Oxidation (M)
2568 564.9894 1691.9464 1691.9206 15.2 0 8 0.56 +1Score > 35 indicates identity
Score > 18 indicates homology
TVTTGNPVRPPVLAAAE
2034 482.6028 1444.7866 1444.8051 -12.9 1 8 0.93 +1Score > 39 indicates identity
Score > 21 indicates homology
LRVLHGHSNWVK
2545 558.9852 1673.9338 1673.9577 -14.3 1 8 0.91 +1Score > 36 indicates identity
Score > 20 indicates homology
VAIRHVAPVSLQDLR + Deamidated (NQ)
2894 625.9719 1874.8939 1874.9110 -9.12 1 8 0.63 +1Score > 40 indicates identity
Score > 19 indicates homology
QIEVTTDGAGVSGTVVDVE
1418 578.3038 1154.5930 1154.6084 -13.3 0 8 0.96 +1Score > 40 indicates identity
Score > 21 indicates homology
GNIYVYASLR
2711 586.9846 1757.9320 1757.9159 9.14 1 8 0.53 +1Score > 39 indicates identity
Score > 18 indicates homology
IARLQNVLDLTGSDNK + 2 Deamidated (NQ)
3017 654.6541 1960.9405 1960.9412 -0.36 1 8 0.57 +1Score > 40 indicates identity
Score > 18 indicates homology
VNGGLLVQDRDLGMVTAGE + 2 Deamidated (NQ); Oxidation (M)
1414 578.3000 1154.5854 1154.6084 -19.9 0 8 0.49 +1Score > 40 indicates identity
Score > 18 indicates homology
GNIYVYASLR
3706 788.1188 2361.3346 2361.3743 -16.8 1 8 0.36 +1Score > 30 indicates identity
Score > 16 indicates homology
NIRLSVAAIVTDVLPNGNLIIR + Deamidated (NQ)
1676 637.3342 1272.6538 1272.6786 -19.5 1 8 0.93 +1Score > 42 indicates identity
Score > 20 indicates homology
RAVVNDLAGTTR + Deamidated (NQ)
2757 596.3293 1785.9661 1785.9910 -14.0 1 8 0.54 +1Score > 38 indicates identity
Score > 18 indicates homology
SAGKGMPVLLLTALGTIE + Oxidation (M)
3357 1081.0403 2160.0660 2160.0305 16.5 1 8 0.53 +1Score > 39 indicates identity
Score > 18 indicates homology
MAPQIDYTMMVFKPIAEK + 3 Oxidation (M)
425 710.3892 709.3819 709.3871 -7.32 0 8 0.31 +1Score > 37 indicates identity
Score > 16 indicates homology
IGDHIR
2250 775.3806 1548.7466 1548.7267 12.9 1 8 0.86 +1Score > 41 indicates identity
Score > 20 indicates homology
GTDNSNDAIKSALNK + 2 Deamidated (NQ)
3290 1064.6145 2127.2144 2127.1728 19.6 1 8 0.24 +1Score > 30 indicates identity
Score > 15 indicates homology
LAALGNIYVNDQAPWKLLK + Deamidated (NQ)
2797 603.9520 1808.8342 1808.8033 17.1 1 8 0.61 +1Score > 40 indicates identity
Score > 19 indicates homology
QAITSAGSGCMAALDAER + Deamidated (NQ)
2539 558.6191 1672.8355 1672.8460 -6.32 1 8 0.63 +1Score > 41 indicates identity
Score > 19 indicates homology
GYNAVYFKAALSGALE
2566 846.4769 1690.9392 1690.9155 14.1 0 8 0.44 +1Score > 35 indicates identity
Score > 17 indicates homology
ILQLAGQPFDHSPIR
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