MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2145 752.3910 1502.7674 1502.7477 13.1 1 10 0.92 +1Score > 41 indicates identity
Score > 22 indicates homology
NSIHSIYTELNGR
2992 648.0267 1941.0583 1941.0836 -13.0 0 10 0.99 +1Score > 36 indicates identity
Score > 22 indicates homology
AIILVSLPFNASWLNQR
3441 733.3934 2197.1584 2197.1338 11.2 1 10 0.35 +1Score > 37 indicates identity
Score > 18 indicates homology
SLTSAQLQRLTNLHGAASLSE + Deamidated (NQ)
3535 1128.6194 2255.2242 2255.2426 -8.14 1 10 0.74 +1Score > 35 indicates identity
Score > 21 indicates homology
KPLSEISFGHVLLNLFNTAR
1885 458.2670 1371.7792 1371.7609 13.3 1 10 0.82 +1Score > 37 indicates identity
Score > 21 indicates homology
QAVELSALLAQTK + Deamidated (NQ)
2429 812.4273 1622.8400 1622.8416 -0.97 0 10 0.72 +1Score > 40 indicates identity
Score > 21 indicates homology
HGVQLNAPAAFQLVR + 3 Deamidated (NQ)
1402 384.1886 1149.5440 1149.5410 2.62 1 10 0.94 +1Score > 42 indicates identity
Score > 22 indicates homology
KSGMIPLQME + Deamidated (NQ); Oxidation (M)
2137 750.4086 1498.8026 1498.7814 14.2 1 10 0.32 +1Score > 39 indicates identity
Score > 17 indicates homology
DGKTPIQLVQMPR + Deamidated (NQ); Oxidation (M)
2286 392.4604 1565.8125 1565.8236 -7.07 0 10 0.56 +1Score > 40 indicates identity
Score > 20 indicates homology
IVVGMSGASGAIYGIR + Oxidation (M)
3025 657.3699 1969.0879 1969.0745 6.78 0 10 0.64 +1Score > 35 indicates identity
Score > 20 indicates homology
TVNIPSYQVRPGDVVAVR
4269 961.1341 2880.3805 2880.3851 -1.61 1 10 0.38 +1Score > 37 indicates identity
Score > 18 indicates homology
TAINAPNNYQVAVQIFDGKTSLCEPK + 3 Deamidated (NQ)
866 453.7519 905.4892 905.4858 3.80 1 10 0.9 +1Score > 41 indicates identity
Score > 22 indicates homology
AADEFLLK
1110 505.2170 1008.4194 1008.4070 12.4 1 10 0.88 +1Score > 39 indicates identity
Score > 22 indicates homology
NVEAQNGMK + 3 Deamidated (NQ); Oxidation (M)
763 863.4486 862.4413 862.4470 -6.59 0 10 0.55 +1Score > 42 indicates identity
Score > 19 indicates homology
TGVLITME
1559 609.8651 1217.7156 1217.7019 11.3 0 10 0.95 +1Score > 34 indicates identity
Score > 22 indicates homology
FSLANLAIQIK + Deamidated (NQ)
2596 427.7368 1706.9181 1706.8879 17.7 1 10 0.67 +1Score > 38 indicates identity
Score > 20 indicates homology
GLNPYKLVINQTAFE + Deamidated (NQ)
280 318.6908 635.3670 635.3676 -0.89 1 10 0.76 +1Score > 39 indicates identity
Score > 21 indicates homology
MTKIK + Oxidation (M)
3666 1170.6028 2339.1910 2339.1757 6.57 1 10 0.9 +1Score > 38 indicates identity
Score > 22 indicates homology
LYTNVRPEASYNQSLSILDR + Deamidated (NQ)
2828 608.9922 1823.9548 1823.9417 7.14 1 10 1 +1Score > 38 indicates identity
Score > 22 indicates homology
TAQKLAFQAAVSDAFQK + Deamidated (NQ)
3134 679.3928 2035.1566 2035.1605 -1.93 1 10 0.53 +1Score > 31 indicates identity
Score > 19 indicates homology
VQLVLPLLVAEAAAAPAFLE + Deamidated (NQ)
2687 872.4658 1742.9170 1742.9090 4.59 1 10 0.71 +1Score > 39 indicates identity
Score > 21 indicates homology
ELQTVIYHPNLSTVK + 2 Deamidated (NQ)
1404 384.2132 1149.6178 1149.6142 3.07 1 10 0.97 +1Score > 40 indicates identity
Score > 22 indicates homology
KGTVITFGDGR
1044 979.5121 978.5048 978.5242 -19.8 0 10 0.55 +1Score > 41 indicates identity
Score > 19 indicates homology
SILMLGAMK + Oxidation (M)
1371 379.8907 1136.6503 1136.6441 5.41 1 10 0.6 +1Score > 36 indicates identity
Score > 20 indicates homology
KFSDSIITVK
1974 710.3811 1418.7476 1418.7228 17.5 0 10 0.86 +1Score > 40 indicates identity
Score > 21 indicates homology
TTGTPVSMVVWNK
2000 715.8701 1429.7256 1429.7412 -10.9 1 10 0.8 +1Score > 41 indicates identity
Score > 21 indicates homology
ANSINSKLNILNE + Deamidated (NQ)
2727 884.4381 1766.8616 1766.8435 10.3 1 10 0.47 +1Score > 40 indicates identity
Score > 19 indicates homology
LQHSGSTNKQQQSPPK + 3 Deamidated (NQ)
1076 995.5157 994.5084 994.5196 -11.2 0 9 0.65 +1Score > 41 indicates identity
Score > 20 indicates homology
IGHTISDPR
3570 760.0062 2276.9968 2277.0132 -7.19 1 9 0.38 +1Score > 36 indicates identity
Score > 18 indicates homology
LTTQAQNVVAEAQAQATSAGNAE + 5 Deamidated (NQ)
1687 639.3710 1276.7274 1276.7101 13.6 0 9 0.35 +1Score > 36 indicates identity
Score > 17 indicates homology
MLQVLIYIVGE
4205 939.5164 2815.5274 2815.5153 4.29 1 9 1 +1Score > 33 indicates identity
Score > 22 indicates homology
IINGGLTLMNVKNIIIHNINIHDVK + 4 Deamidated (NQ); Oxidation (M)
4281 964.8893 2891.6461 2891.5949 17.7 1 9 1 +1Score > 27 indicates identity
Score > 22 indicates homology
QLFLYKVTIPALASQNPAIHPFTPPK + Deamidated (NQ)
517 757.3882 756.3809 756.3766 5.73 1 9 0.71 +1Score > 38 indicates identity
Score > 20 indicates homology
NGIPAER + Deamidated (NQ)
948 469.7344 937.4542 937.4393 16.0 0 9 0.94 +1Score > 41 indicates identity
Score > 22 indicates homology
YSQLQGIE + Deamidated (NQ)
2585 426.9737 1703.8657 1703.8777 -7.05 1 9 0.34 +1Score > 41 indicates identity
Score > 17 indicates homology
QGAIIMHPAPVNRGVE + Oxidation (M)
3311 712.7105 2135.1097 2135.0687 19.2 0 9 0.82 +1Score > 38 indicates identity
Score > 21 indicates homology
AQWFTIQHIQPSPLQPNK + 3 Deamidated (NQ)
3827 825.7653 2474.2741 2474.2522 8.83 1 9 0.96 +1Score > 38 indicates identity
Score > 22 indicates homology
MPITQQMHAILHQGKSPSDAIR + Oxidation (M)
1816 446.2581 1335.7525 1335.7663 -10.3 1 9 1 +1Score > 36 indicates identity
Score > 22 indicates homology
LLLFLDAYRGR
3071 665.6497 1993.9273 1993.9231 2.12 1 9 0.43 +1Score > 39 indicates identity
Score > 18 indicates homology
TVISSSMFFPLYETLNE + Deamidated (NQ); Oxidation (M)
1954 705.3418 1408.6690 1408.6656 2.41 1 9 0.96 +1Score > 41 indicates identity
Score > 22 indicates homology
EMVQAIVDYQGR + Deamidated (NQ)
3844 831.4284 2491.2634 2491.3006 -14.9 1 9 0.45 +1Score > 38 indicates identity
Score > 18 indicates homology
TPQTLVGGPVAMQRWLRPPDVR + 2 Deamidated (NQ); Oxidation (M)
3027 986.0229 1970.0312 1970.0255 2.93 1 9 0.89 +1Score > 38 indicates identity
Score > 21 indicates homology
TSQQIILNGQAVRMPDAK + Deamidated (NQ)
3349 718.7170 2153.1292 2153.1633 -15.8 0 9 0.32 +1Score > 38 indicates identity
Score > 17 indicates homology
RPQIQTIAPGAQIAHFLYK + 2 Deamidated (NQ)
2509 833.9930 1665.9714 1665.9413 18.1 1 9 0.19 +1Score > 32 indicates identity
Score > 15 indicates homology
QQLINLDQVNKKPK + Deamidated (NQ)
1527 402.8579 1205.5519 1205.5598 -6.56 1 9 0.55 +1Score > 40 indicates identity
Score > 19 indicates homology
DLIEAMDDLR + Oxidation (M)
1796 442.9140 1325.7202 1325.7092 8.29 1 9 0.55 +1Score > 39 indicates identity
Score > 19 indicates homology
TVGTASAFFAKAR
1693 427.5928 1279.7566 1279.7500 5.17 1 9 0.93 +1Score > 34 indicates identity
Score > 22 indicates homology
SVHDILINLKK + Deamidated (NQ)
4569 832.9202 3327.6517 3327.6626 -3.29 1 9 0.56 +1Score > 35 indicates identity
Score > 19 indicates homology
QCVYIRFDMLVMAIGGMIALINPTNNIIK + 4 Deamidated (NQ); Oxidation (M)
3253 705.6987 2114.0743 2114.0756 -0.62 1 9 0.48 +1Score > 39 indicates identity
Score > 19 indicates homology
LTNSIIWNSPNVSINRQR + 3 Deamidated (NQ)
3762 806.4193 2416.2361 2416.2611 -10.4 1 9 0.52 +1Score > 38 indicates identity
Score > 19 indicates homology
GYDAPRPGLDFRLLITGGSQGAR
2243 515.9443 1544.8111 1544.7868 15.7 1 9 0.83 +1Score > 40 indicates identity
Score > 21 indicates homology
RMTLSGQLEPTLAE
341 336.1983 670.3820 670.3762 8.72 0 9 0.51 +1Score > 35 indicates identity
Score > 19 indicates homology
AGLPSAR
3314 712.7120 2135.1142 2135.0833 14.4 1 9 0.38 +1Score > 38 indicates identity
Score > 18 indicates homology
LVHHDIIDALENMSRPFK + Deamidated (NQ)
3255 1058.5381 2115.0616 2115.0592 1.17 0 9 0.57 +1Score > 39 indicates identity
Score > 19 indicates homology
TAIIDPTDMLSMPLPSGNVK + Oxidation (M)
2374 534.2763 1599.8071 1599.8216 -9.11 1 9 0.72 +1Score > 40 indicates identity
Score > 20 indicates homology
ATVSQTISTEIASHR
4497 1061.5258 3181.5556 3181.5682 -3.97 1 9 1 +1Score > 36 indicates identity
Score > 22 indicates homology
LAMHGTHNMLNSLAAIGVATQMGIADDKIR + Deamidated (NQ); 2 Oxidation (M)
1557 406.5696 1216.6870 1216.6928 -4.79 1 9 0.5 +1Score > 38 indicates identity
Score > 19 indicates homology
KHPDNVVPALK
3179 1030.5348 2059.0550 2059.0545 0.26 1 9 0.82 +1Score > 39 indicates identity
Score > 21 indicates homology
IVSSQINSGNALSADVQKAR + 2 Deamidated (NQ)
3613 769.7367 2306.1883 2306.1729 6.66 1 9 0.37 +1Score > 38 indicates identity
Score > 17 indicates homology
FTKMHGLGNDFVVLDLISQR + Deamidated (NQ); Oxidation (M)
2643 864.4917 1726.9688 1726.9352 19.5 1 9 0.65 +1Score > 36 indicates identity
Score > 20 indicates homology
AIIDAILAIQDKISNE + Deamidated (NQ)
2069 489.6165 1465.8277 1465.8253 1.65 1 9 0.51 +1Score > 36 indicates identity
Score > 19 indicates homology
QLHQRVSVILGSK + 2 Deamidated (NQ)
4612 859.4423 3433.7401 3433.7360 1.19 1 9 0.19 +1Score > 35 indicates identity
Score > 15 indicates homology
AVGTGTSLALSSLLSLLLFAGMQMYSRQLASTE + 2 Deamidated (NQ); Oxidation (M)
604 395.7280 789.4414 789.4385 3.76 0 9 0.6 +1Score > 40 indicates identity
Score > 19 indicates homology
SPLFAGAK
3304 1068.0969 2134.1792 2134.1422 17.4 1 9 0.9 +1Score > 34 indicates identity
Score > 21 indicates homology
KQILIEPVSAQWIQSAHGK + 2 Deamidated (NQ)
1373 569.8068 1137.5990 1137.6070 -7.00 0 9 0.62 +1Score > 40 indicates identity
Score > 20 indicates homology
ADFFLLDGIK
2346 793.3593 1584.7040 1584.7016 1.55 0 9 0.71 +1Score > 39 indicates identity
Score > 20 indicates homology
VGSNSQDGDATPSPPR + Deamidated (NQ)
2769 600.3356 1797.9850 1797.9837 0.73 0 9 0.16 +1Score > 36 indicates identity
Score > 14 indicates homology
VTVSTSGVVPALDILGDR
3080 501.2628 2001.0221 2000.9976 12.2 1 9 0.72 +1Score > 39 indicates identity
Score > 20 indicates homology
QLMELDTSPSPSAQVVGLK + 2 Deamidated (NQ)
2366 798.4111 1594.8076 1594.7951 7.89 0 9 0.6 +1Score > 40 indicates identity
Score > 19 indicates homology
QQLANLTHLNDAQK + 2 Deamidated (NQ)
2800 905.9919 1809.9692 1809.9724 -1.72 1 9 0.75 +1Score > 38 indicates identity
Score > 20 indicates homology
DPAIRNVIVLQTVLQE + 3 Deamidated (NQ)
3491 746.3914 2236.1524 2236.1232 13.1 0 9 0.79 +1Score > 38 indicates identity
Score > 21 indicates homology
AIIATVLQMLMGGGGSFSAGGPGK + Deamidated (NQ); Oxidation (M)
3829 826.0746 2475.2020 2475.2113 -3.76 0 9 0.51 +1Score > 38 indicates identity
Score > 19 indicates homology
SFVYGTGCGLGWMLAIISMAGLR + Oxidation (M)
3070 665.3533 1993.0381 1993.0561 -9.03 1 9 0.53 +1Score > 38 indicates identity
Score > 19 indicates homology
IIPKYDYVVLNGPAGVFE
3967 522.6483 2608.2051 2608.2380 -12.6 0 9 0.28 +1Score > 37 indicates identity
Score > 16 indicates homology
SNRPPMGWPSLTNSNHTPPIIFE + Deamidated (NQ); Oxidation (M)
2742 887.4678 1772.9210 1772.8866 19.4 0 9 0.61 +1Score > 40 indicates identity
Score > 19 indicates homology
LNTILNTMSTIYSTGK + Deamidated (NQ); Oxidation (M)
2914 944.4391 1886.8636 1886.8720 -4.43 1 9 0.95 +1Score > 39 indicates identity
Score > 21 indicates homology
DDICVFISEHNPAQIK + 2 Deamidated (NQ)
2423 809.3979 1616.7812 1616.8046 -14.5 1 9 0.45 +1Score > 40 indicates identity
Score > 18 indicates homology
IYVKHTGQDLDTVE
2063 366.4663 1461.8361 1461.8113 17.0 1 9 0.91 +1Score > 35 indicates identity
Score > 21 indicates homology
MTVLTQEAIITVK + Oxidation (M)
1347 564.3451 1126.6756 1126.6710 4.13 0 9 0.69 +1Score > 36 indicates identity
Score > 20 indicates homology
ITLQGINIVR + Deamidated (NQ)
1487 592.3506 1182.6866 1182.6873 -0.57 1 9 0.58 +1Score > 37 indicates identity
Score > 19 indicates homology
HLLVPRGTYK
2751 594.6810 1781.0212 1781.0159 2.94 1 9 0.15 +1Score > 32 indicates identity
Score > 13 indicates homology
RLGLGLQGSVQVLPSTR + Deamidated (NQ)
2971 644.3698 1930.0876 1930.0847 1.47 1 9 0.4 +1Score > 33 indicates identity
Score > 18 indicates homology
IASSGSVLVSGRLLSDITR
4291 724.8915 2895.5369 2895.5454 -2.94 1 9 0.47 +1Score > 34 indicates identity
Score > 18 indicates homology
TPHIQNLAVGGVANPINLDGLGVLNLER + 2 Deamidated (NQ)
1282 365.5259 1093.5559 1093.5404 14.2 0 9 0.93 +1Score > 41 indicates identity
Score > 21 indicates homology
NSYLIDNVR + Deamidated (NQ)
2915 944.5095 1887.0044 1887.0102 -3.03 1 9 0.96 +1Score > 38 indicates identity
Score > 21 indicates homology
FGRLVQLQTLILQDNE + Deamidated (NQ)
2156 755.4089 1508.8032 1508.8021 0.76 1 9 0.54 +1Score > 39 indicates identity
Score > 19 indicates homology
APLHETLLDMVVR + Oxidation (M)
360 678.3282 677.3209 677.3166 6.31 0 9 0.7 +1Score > 41 indicates identity
Score > 20 indicates homology
LMGDAR + Oxidation (M)
1132 508.2937 1014.5728 1014.5709 1.88 1 9 0.75 +1Score > 41 indicates identity
Score > 20 indicates homology
VLEQLIGSR + Deamidated (NQ)
3493 746.4081 2236.2025 2236.2038 -0.60 1 9 0.2 +1Score > 36 indicates identity
Score > 14 indicates homology
KVNSAVFPGLQGGPLMHVIAGK + Deamidated (NQ); Oxidation (M)
4008 885.7870 2654.3392 2654.2931 17.3 1 9 0.26 +1Score > 37 indicates identity
Score > 16 indicates homology
VQADYMGMLATVINSLALQDALER + Deamidated (NQ); 2 Oxidation (M)
3387 544.2697 2173.0497 2173.0474 1.07 1 9 0.88 +1Score > 39 indicates identity
Score > 21 indicates homology
YRVTLDNGVVVGAYASGQMR + 2 Deamidated (NQ); Oxidation (M)
2933 953.0218 1904.0290 1904.0255 1.88 1 9 0.7 +1Score > 37 indicates identity
Score > 20 indicates homology
DIAKVAPANTAVQVIAPPE + Deamidated (NQ)
2536 836.4700 1670.9254 1670.9025 13.7 1 9 0.51 +1Score > 37 indicates identity
Score > 19 indicates homology
NIIPLSDMTVVRADK
3557 758.0974 2271.2704 2271.2699 0.21 1 9 0.16 +1Score > 32 indicates identity
Score > 14 indicates homology
GINIVTKHNKPTANVPNGGIVK + Deamidated (NQ)
1890 690.3369 1378.6592 1378.6737 -10.5 0 9 0.78 +1Score > 41 indicates identity
Score > 20 indicates homology
NISGVVMHMTFK + Oxidation (M)
2921 946.4803 1890.9460 1890.9218 12.8 1 9 0.9 +1Score > 40 indicates identity
Score > 21 indicates homology
DDVAASMALRIAGSSGQAR + Oxidation (M)
3691 787.0750 2358.2032 2358.1676 15.1 1 9 0.18 +1Score > 38 indicates identity
Score > 14 indicates homology
AGAATPRTAPGHSTPAVTGANQPAR + 2 Deamidated (NQ)
3301 1068.0288 2134.0430 2134.0039 18.3 1 9 0.44 +1Score > 40 indicates identity
Score > 18 indicates homology
QVDSSSVLHNASTRFSDGAR + Deamidated (NQ)
3483 744.3752 2230.1038 2230.1450 -18.5 1 9 0.41 +1Score > 39 indicates identity
Score > 18 indicates homology
AIIDTALSGRDCLVVMPTGGGK
4005 663.0624 2648.2205 2648.2429 -8.44 0 9 0.79 +1Score > 37 indicates identity
Score > 20 indicates homology
VGAVDMTQHQSVGGPYNVQGFPTLK + 3 Deamidated (NQ); Oxidation (M)
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