MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2301 786.4405 1570.8664 1570.8541 7.85 0 10 0.53 +1Score > 38 indicates identity
Score > 20 indicates homology
LAGILVNDWMAVIR + Deamidated (NQ)
2659 578.6556 1732.9450 1732.9108 19.7 1 10 0.77 +1Score > 37 indicates identity
Score > 22 indicates homology
TFQSGADADLLAQLKR
2945 638.9907 1913.9503 1913.9384 6.21 1 10 0.96 +1Score > 40 indicates identity
Score > 23 indicates homology
GYAQHGHGTEALLLYQR + Deamidated (NQ)
3505 1122.6045 2243.1944 2243.2249 -13.6 1 10 0.18 +1Score > 37 indicates identity
Score > 15 indicates homology
NANCLFIHLTLLPYVKTAR
3883 632.8280 2527.2829 2527.2741 3.50 1 10 0.26 +1Score > 38 indicates identity
Score > 17 indicates homology
AVDVMNQNVPNSQQLLFPQKIE + Oxidation (M)
2899 940.4943 1878.9740 1878.9588 8.11 0 10 0.56 +1Score > 39 indicates identity
Score > 20 indicates homology
QLVANFDIHDVLGNPAR + Deamidated (NQ)
2589 569.3268 1704.9586 1704.9273 18.3 1 10 0.29 +1Score > 34 indicates identity
Score > 17 indicates homology
GVFIFLMPPSLAELR + Oxidation (M)
1595 616.8552 1231.6958 1231.6812 11.9 1 10 0.86 +1Score > 38 indicates identity
Score > 22 indicates homology
LQNIFQEVLK + Deamidated (NQ)
2619 860.4608 1718.9070 1718.8938 7.69 1 10 0.89 +1Score > 39 indicates identity
Score > 22 indicates homology
GTEVIIITTDTSPSGTK
2908 943.0076 1884.0006 1883.9993 0.72 0 10 0.63 +1Score > 38 indicates identity
Score > 21 indicates homology
LFNVVVTTAGALDHDIAK + Deamidated (NQ)
1040 325.8846 974.6320 974.6164 15.9 1 10 0.4 +1Score > 27 indicates identity
Score > 19 indicates homology
KDIVLFLK
3511 749.0327 2244.0763 2244.1096 -14.9 1 10 0.72 +1Score > 39 indicates identity
Score > 21 indicates homology
LKTDLSDPDPSQIDWVMLR + Oxidation (M)
2129 748.8736 1495.7326 1495.7051 18.4 1 10 0.98 +1Score > 41 indicates identity
Score > 23 indicates homology
EMCLSQLFLQVE
2303 524.9497 1571.8273 1571.8532 -16.5 1 10 0.8 +1Score > 40 indicates identity
Score > 22 indicates homology
VHQIGSNLHIEAVR
1416 578.3029 1154.5912 1154.6084 -14.8 0 10 0.96 +1Score > 40 indicates identity
Score > 23 indicates homology
GNIYVYASLR
1490 593.3220 1184.6294 1184.6401 -8.97 0 10 0.97 +1Score > 40 indicates identity
Score > 23 indicates homology
NTIANIQGLIE
1677 637.3359 1272.6572 1272.6714 -11.1 1 10 0.84 +1Score > 41 indicates identity
Score > 22 indicates homology
ILEALPNAQFR + 2 Deamidated (NQ)
3014 980.0195 1958.0244 1957.9938 15.6 0 10 0.73 +1Score > 39 indicates identity
Score > 21 indicates homology
FLSQPFFVAAQFTGVDGK
1659 421.5620 1261.6642 1261.6666 -1.96 1 10 0.87 +1Score > 41 indicates identity
Score > 22 indicates homology
AEIGAVDAIFTR
1190 518.3010 1034.5874 1034.6012 -13.3 1 10 0.74 +1Score > 37 indicates identity
Score > 21 indicates homology
IEIYIVGTK
2484 551.6431 1651.9075 1651.8855 13.3 1 10 0.32 +1Score > 37 indicates identity
Score > 18 indicates homology
ADVMTVAATLLKQFK + Deamidated (NQ); Oxidation (M)
2925 631.9985 1892.9737 1892.9632 5.51 1 10 0.98 +1Score > 39 indicates identity
Score > 23 indicates homology
FNNKTGVIIFTDPDGAGK
3419 547.7708 2187.0541 2187.0430 5.07 1 10 0.58 +1Score > 39 indicates identity
Score > 20 indicates homology
QNDAINGDIVAALLDDEATVK + 3 Deamidated (NQ)
3248 704.3731 2110.0975 2110.0721 12.0 1 10 0.26 +1Score > 38 indicates identity
Score > 17 indicates homology
TSLNESIIFDILQLDFIE + Deamidated (NQ)
3361 541.0252 2160.0717 2160.0997 -13.0 1 10 0.35 +1Score > 39 indicates identity
Score > 18 indicates homology
GISDLAQHYLMRANITAIR + 2 Deamidated (NQ); Oxidation (M)
886 458.2755 914.5364 914.5185 19.6 0 10 0.84 +1Score > 41 indicates identity
Score > 22 indicates homology
IAALADSVR
2174 379.4685 1513.8449 1513.8504 -3.64 1 10 0.97 +1Score > 38 indicates identity
Score > 22 indicates homology
TSLLIPNPKQFVR + 2 Deamidated (NQ)
2561 844.4805 1686.9464 1686.9417 2.82 0 10 0.21 +1Score > 35 indicates identity
Score > 16 indicates homology
VPKPDQDLVLLSAHR
1499 596.2913 1190.5680 1190.5819 -11.6 1 10 0.97 +1Score > 41 indicates identity
Score > 22 indicates homology
VQQAEDFKPK + 2 Deamidated (NQ)
2297 524.6108 1570.8106 1570.8137 -1.99 1 10 0.74 +1Score > 40 indicates identity
Score > 21 indicates homology
ISQMLDISDKLHR + Oxidation (M)
2480 825.9303 1649.8460 1649.8624 -9.94 0 10 0.66 +1Score > 40 indicates identity
Score > 21 indicates homology
ITTVQAAIDFIQSSR + Deamidated (NQ)
3797 816.7578 2447.2516 2447.2471 1.81 1 10 0.22 +1Score > 38 indicates identity
Score > 16 indicates homology
IKWGQYVTQNQILGINDVLIE + 4 Deamidated (NQ)
1936 467.5781 1399.7125 1399.7195 -4.99 0 10 0.67 +1Score > 40 indicates identity
Score > 21 indicates homology
QAVTNPNVVQSIK + 3 Deamidated (NQ)
2677 581.6476 1741.9210 1741.9443 -13.4 1 10 0.55 +1Score > 39 indicates identity
Score > 20 indicates homology
VFTKDFQAVLWLFK + Deamidated (NQ)
3040 990.5198 1979.0250 1979.0372 -6.16 1 10 0.96 +1Score > 39 indicates identity
Score > 22 indicates homology
FLKCLGPNLIFDMGLNK
3170 687.0247 2058.0523 2058.0343 8.72 1 10 0.2 +1Score > 39 indicates identity
Score > 16 indicates homology
MLPPLVTGAPLSFLESQNE + Oxidation (M)
3653 776.4148 2326.2226 2326.1804 18.1 1 10 0.81 +1Score > 37 indicates identity
Score > 22 indicates homology
LGNNLPTPQEVVALYNQANIR + 3 Deamidated (NQ)
3708 788.7238 2363.1496 2363.1063 18.3 1 10 0.67 +1Score > 39 indicates identity
Score > 21 indicates homology
NVEYVADLGCGPGNSTALLQQR + 2 Deamidated (NQ)
2187 507.2936 1518.8590 1518.8293 19.5 1 10 0.39 +1Score > 36 indicates identity
Score > 18 indicates homology
TANIALLVYEDGIK
2601 855.4160 1708.8174 1708.8156 1.11 1 10 0.43 +1Score > 41 indicates identity
Score > 19 indicates homology
VPSTTALESYNTVAQE
2171 505.2854 1512.8344 1512.8147 13.0 1 10 0.43 +1Score > 38 indicates identity
Score > 19 indicates homology
LTLSKAAAQQLDPR + 2 Deamidated (NQ)
3163 684.6901 2051.0485 2051.0827 -16.7 0 10 0.74 +1Score > 39 indicates identity
Score > 21 indicates homology
TNLINLGVVIGTLLYFGNE + 2 Deamidated (NQ)
1543 404.8766 1211.6080 1211.6033 3.82 0 10 0.79 +1Score > 40 indicates identity
Score > 22 indicates homology
IPVLNNNLDAE + Deamidated (NQ)
2456 817.9384 1633.8622 1633.8861 -14.6 1 10 0.54 +1Score > 39 indicates identity
Score > 20 indicates homology
ARYMPQKPTIIATK + Deamidated (NQ); Oxidation (M)
2740 591.3172 1770.9298 1770.9298 -0.021 1 10 0.98 +1Score > 39 indicates identity
Score > 22 indicates homology
LSNVTVGAGMIRQPLAE + Oxidation (M)
704 842.4369 841.4296 841.4294 0.31 1 10 0.79 +1Score > 38 indicates identity
Score > 21 indicates homology
RLGVNPGE + Deamidated (NQ)
3159 683.7112 2048.1118 2048.1201 -4.04 0 10 0.43 +1Score > 36 indicates identity
Score > 19 indicates homology
SLLQMQKPAQGAPLTVNPR
2846 615.3513 1843.0321 1843.0567 -13.4 1 10 0.44 +1Score > 35 indicates identity
Score > 19 indicates homology
ISDQLVILAGKNGFVLR + Deamidated (NQ)
3099 672.6998 2015.0776 2015.0509 13.2 1 10 0.71 +1Score > 37 indicates identity
Score > 21 indicates homology
NLPMASSAIIWATQLKNR + 2 Deamidated (NQ)
3237 1051.5266 2101.0386 2101.0375 0.57 1 10 0.69 +1Score > 40 indicates identity
Score > 21 indicates homology
NAGKAVHIMGLLSAGGVHSHE + Deamidated (NQ); Oxidation (M)
3732 796.0557 2385.1453 2385.1311 5.93 0 10 0.28 +1Score > 39 indicates identity
Score > 17 indicates homology
SYLSVGGVSMGIAGSIVDHNFFE
1609 413.2366 1236.6880 1236.6938 -4.74 1 10 0.66 +1Score > 37 indicates identity
Score > 21 indicates homology
HLKNGNATLLR + Deamidated (NQ)
1639 627.3409 1252.6672 1252.6485 14.9 1 10 0.89 +1Score > 39 indicates identity
Score > 22 indicates homology
TQIFCITKNK + Deamidated (NQ)
2918 630.3438 1888.0096 1888.0240 -7.67 0 10 0.32 +1Score > 38 indicates identity
Score > 18 indicates homology
SIMTLRPAPVNTGIVFR + Deamidated (NQ); Oxidation (M)
69 389.2170 388.2097 388.2070 6.94 0 10 0.21 +1Score > 44 indicates identity
Score > 16 indicates homology
AGGGK
2764 597.6417 1789.9033 1789.9008 1.40 1 10 0.29 +1Score > 40 indicates identity
Score > 17 indicates homology
MMRVSVVPFFQPHSK + Deamidated (NQ)
3360 721.0305 2160.0697 2160.0997 -13.9 1 10 0.39 +1Score > 39 indicates identity
Score > 18 indicates homology
GISDLAQHYLMRANITAIR + 2 Deamidated (NQ); Oxidation (M)
4054 900.1725 2697.4957 2697.4483 17.6 1 10 0.23 +1Score > 31 indicates identity
Score > 16 indicates homology
QVMVNINNAAAVNAKNLIGSVLLASR + 2 Deamidated (NQ); Oxidation (M)
2922 631.6866 1892.0380 1892.0367 0.66 0 10 0.72 +1Score > 36 indicates identity
Score > 21 indicates homology
RPSSLVHSIVGQVITGIE + Deamidated (NQ)
3856 835.0833 2502.2281 2502.2424 -5.74 1 10 1 +1Score > 38 indicates identity
Score > 22 indicates homology
QPMGVVVQFGGQTAINLADELSAR + 2 Deamidated (NQ)
4090 683.3943 2729.5481 2729.5004 17.5 1 10 1 +1Score > 28 indicates identity
Score > 22 indicates homology
VVELLLPALGYPGAIVVDATVGLGGHAE
1616 413.9044 1238.6914 1238.6731 14.7 1 10 0.83 +1Score > 38 indicates identity
Score > 22 indicates homology
QRVAADLPVDR
1824 671.3841 1340.7536 1340.7551 -1.09 0 10 0.43 +1Score > 38 indicates identity
Score > 19 indicates homology
LNPSLVISLSQGL + Deamidated (NQ)
2657 578.5963 1732.7671 1732.7951 -16.2 1 10 0.26 +1Score > 38 indicates identity
Score > 17 indicates homology
HNQSPRNTYQSAMAK + Deamidated (NQ)
429 714.3870 713.3797 713.3708 12.5 0 10 0.18 +1Score > 38 indicates identity
Score > 15 indicates homology
GVQAGPGK + Deamidated (NQ)
2948 958.0421 1914.0696 1914.0422 14.3 1 10 0.58 +1Score > 34 indicates identity
Score > 20 indicates homology
LSDLTLQDSQKVVVVNR + Deamidated (NQ)
3876 841.7883 2522.3431 2522.3090 13.5 1 10 0.57 +1Score > 35 indicates identity
Score > 20 indicates homology
SLTLSHNNMIALKPILQAWLEE + 2 Deamidated (NQ)
2641 576.6257 1726.8553 1726.8672 -6.92 0 10 0.87 +1Score > 40 indicates identity
Score > 22 indicates homology
LPVSMPSGNQQPSLTR + Oxidation (M)
1383 570.8441 1139.6736 1139.6550 16.4 0 10 0.52 +1Score > 36 indicates identity
Score > 19 indicates homology
QLATVEPALAK
2214 766.8921 1531.7696 1531.7552 9.44 0 10 0.8 +1Score > 41 indicates identity
Score > 21 indicates homology
IVLNMGVGDATQNAK + 2 Deamidated (NQ)
2512 834.4412 1666.8678 1666.8665 0.79 0 10 1 +1Score > 39 indicates identity
Score > 22 indicates homology
SVPVNNLVQVVGTPLE + 3 Deamidated (NQ)
2649 577.3251 1728.9535 1728.9457 4.47 1 10 0.92 +1Score > 37 indicates identity
Score > 22 indicates homology
VGQSLNIMGWVRTLR
2122 498.9253 1493.7541 1493.7296 16.4 1 10 0.9 +1Score > 41 indicates identity
Score > 22 indicates homology
VQNKLTNPDMYR + Oxidation (M)
591 787.5159 786.5086 786.4963 15.6 1 10 0.86 +1Score > 33 indicates identity
Score > 22 indicates homology
TKAGALVK
2942 638.6932 1913.0578 1913.0404 9.08 1 10 0.66 +1Score > 35 indicates identity
Score > 20 indicates homology
GDLQTMLRDLQLQILR + Deamidated (NQ)
472 367.6967 733.3788 733.3719 9.53 0 10 0.77 +1Score > 42 indicates identity
Score > 21 indicates homology
TSAATGAR
1889 689.3763 1376.7380 1376.7299 5.89 0 10 0.97 +1Score > 39 indicates identity
Score > 22 indicates homology
TNYLGLLAINQR + 2 Deamidated (NQ)
3618 578.0338 2308.1061 2308.0933 5.53 1 10 0.35 +1Score > 39 indicates identity
Score > 18 indicates homology
FIILSDILGDEDHLGDMDFK + Oxidation (M)
2758 894.4982 1786.9818 1786.9763 3.08 1 10 0.69 +1Score > 37 indicates identity
Score > 21 indicates homology
LIDTVIYMHLKAQAR + Oxidation (M)
1359 378.1978 1131.5716 1131.5785 -6.11 1 10 0.98 +1Score > 42 indicates identity
Score > 22 indicates homology
RSPNDIQFR
1872 457.2233 1368.6481 1368.6265 15.8 1 10 0.84 +1Score > 40 indicates identity
Score > 21 indicates homology
TNSGVMTAEIAMK + Deamidated (NQ); Oxidation (M)
3909 638.8049 2551.1905 2551.2377 -18.5 0 10 0.25 +1Score > 38 indicates identity
Score > 16 indicates homology
DMGWTLQTLNHSLGGDVSLHQIK + 2 Deamidated (NQ)
1852 453.5787 1357.7143 1357.7201 -4.32 1 10 0.91 +1Score > 41 indicates identity
Score > 22 indicates homology
TKANGTVQVVSPR + 2 Deamidated (NQ)
2148 752.4182 1502.8218 1502.8358 -9.27 1 10 0.86 +1Score > 38 indicates identity
Score > 22 indicates homology
HPVTPWGKPTKGAK
3150 681.3524 2041.0354 2041.0732 -18.5 0 10 0.51 +1Score > 39 indicates identity
Score > 19 indicates homology
TAAYLDVPNTVILHGIDTK + Deamidated (NQ)
3586 765.4038 2293.1896 2293.2291 -17.2 0 10 0.31 +1Score > 38 indicates identity
Score > 17 indicates homology
ATHLVDHLLAHQQLNDSLLR
2442 816.4171 1630.8196 1630.7950 15.1 1 10 0.89 +1Score > 41 indicates identity
Score > 22 indicates homology
GLDWLSNQLKNANR + 3 Deamidated (NQ)
2477 823.9220 1645.8294 1645.8498 -12.4 0 10 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
NVAAVMVTASLPPFGR + Deamidated (NQ); Oxidation (M)
1153 510.7925 1019.5704 1019.5539 16.3 1 10 0.79 +1Score > 39 indicates identity
Score > 21 indicates homology
QIELLYIE
2988 646.3664 1936.0774 1936.1074 -15.5 0 10 0.28 +1Score > 34 indicates identity
Score > 17 indicates homology
QFLASLVSFLLVVWSVK + Deamidated (NQ)
2645 864.9756 1727.9366 1727.9166 11.6 1 10 0.76 +1Score > 38 indicates identity
Score > 21 indicates homology
GTTVIADQVQGDVRIR + Deamidated (NQ)
2859 463.9964 1851.9565 1851.9214 18.9 1 10 0.78 +1Score > 39 indicates identity
Score > 21 indicates homology
DAGIKQVQPEPPTQDTK + Deamidated (NQ)
3067 664.3621 1990.0645 1990.0670 -1.25 1 10 0.4 +1Score > 38 indicates identity
Score > 18 indicates homology
MTLPINNNIFTVSRLNK + Oxidation (M)
1618 414.5579 1240.6519 1240.6676 -12.7 1 10 0.6 +1Score > 40 indicates identity
Score > 20 indicates homology
TVHFDKPNRK
1705 644.3292 1286.6438 1286.6653 -16.6 1 10 0.81 +1Score > 41 indicates identity
Score > 21 indicates homology
QALEICDALVR
3448 551.7640 2203.0269 2203.0110 7.23 1 10 0.29 +1Score > 39 indicates identity
Score > 17 indicates homology
QSTTYADFIYFTAFEGSVR + Deamidated (NQ)
4350 990.8546 2969.5420 2969.4844 19.4 1 10 0.66 +1Score > 35 indicates identity
Score > 20 indicates homology
YDSIALQQAVINLDVAFSNCFNPKLK + 2 Deamidated (NQ)
996 479.2613 956.5080 956.4974 11.1 1 10 0.25 +1Score > 40 indicates identity
Score > 16 indicates homology
QIHMRTR + Oxidation (M)
4200 704.3672 2813.4397 2813.4170 8.07 1 10 0.29 +1Score > 36 indicates identity
Score > 17 indicates homology
YFSNRPAPTPGNLLPRPNCSIEALK + 2 Deamidated (NQ)
1939 701.3837 1400.7528 1400.7259 19.2 1 10 0.35 +1Score > 40 indicates identity
Score > 18 indicates homology
SSVPNDNITRAVK + Deamidated (NQ)
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