MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2296 524.6095 1570.8067 1570.8137 -4.48 1 11 0.62 +1Score > 40 indicates identity
Score > 21 indicates homology
ISQMLDISDKLHR + Oxidation (M)
2856 617.9893 1850.9461 1850.9673 -11.4 0 11 0.57 +1Score > 39 indicates identity
Score > 21 indicates homology
NVLQVHLNGLMQINDK + Oxidation (M)
1714 645.8577 1289.7008 1289.6762 19.1 0 11 0.9 +1Score > 40 indicates identity
Score > 23 indicates homology
LMQSNATVTAVR
393 349.1718 696.3290 696.3303 -1.81 1 11 0.79 +1Score > 38 indicates identity
Score > 22 indicates homology
NAAEHR
3359 721.0305 2160.0697 2160.0997 -13.9 1 11 0.24 +1Score > 39 indicates identity
Score > 17 indicates homology
GISDLAQHYLMRANITAIR + 2 Deamidated (NQ); Oxidation (M)
3392 726.3904 2176.1494 2176.1416 3.59 1 11 0.89 +1Score > 37 indicates identity
Score > 23 indicates homology
GFQTAEYLLAQGLIDAIVPR + 2 Deamidated (NQ)
1311 369.9029 1106.6869 1106.6924 -4.98 1 11 0.33 +1Score > 30 indicates identity
Score > 19 indicates homology
GKIIISIAHR
126 460.2566 459.2493 459.2441 11.3 0 11 0.27 +1Score > 46 indicates identity
Score > 18 indicates homology
AGANK
4017 887.4606 2659.3600 2659.3129 17.7 1 11 0.17 +1Score > 37 indicates identity
Score > 16 indicates homology
LNQHFFDTLTLNVGGAQAAIIESAE + Deamidated (NQ)
3325 536.5229 2142.0625 2142.0382 11.4 0 11 0.5 +1Score > 39 indicates identity
Score > 20 indicates homology
AIGAGATASVQQNWPDIWQK + 2 Deamidated (NQ)
1308 554.2988 1106.5830 1106.5972 -12.7 0 11 0.79 +1Score > 40 indicates identity
Score > 22 indicates homology
NGLVYITQAK + Deamidated (NQ)
1412 385.5666 1153.6780 1153.6819 -3.40 0 11 0.62 +1Score > 36 indicates identity
Score > 21 indicates homology
SVAIAVTLQPR
4057 901.2078 2700.6016 2700.6306 -10.7 0 11 0.89 +1Score > 23 indicates identity VLSTPPVFLLGLLLALPLGAQGLSGVR
1778 441.2408 1320.7006 1320.6786 16.6 0 11 0.62 +1Score > 40 indicates identity
Score > 21 indicates homology
GPNGDHITLQLR + Deamidated (NQ)
1572 408.5605 1222.6597 1222.6557 3.24 1 11 0.85 +1Score > 39 indicates identity
Score > 23 indicates homology
VIQHLQNELK + 2 Deamidated (NQ)
2868 929.4729 1856.9312 1856.9554 -13.0 0 11 0.56 +1Score > 40 indicates identity
Score > 21 indicates homology
VSQAVSIPIIGMGGIQSAE + Deamidated (NQ)
1390 571.8612 1141.7078 1141.7070 0.70 0 11 0.76 +1Score > 31 indicates identity
Score > 22 indicates homology
ISIQVLQTLK
1569 611.8115 1221.6084 1221.6214 -10.6 1 11 0.81 +1Score > 41 indicates identity
Score > 22 indicates homology
QGKKPHGDAQR + Deamidated (NQ)
3315 712.7349 2135.1829 2135.1813 0.76 0 11 0.89 +1Score > 34 indicates identity
Score > 23 indicates homology
VLTIVAMLNVQNIFYRPK + Deamidated (NQ); Oxidation (M)
1883 457.9184 1370.7334 1370.7340 -0.46 0 11 0.7 +1Score > 40 indicates identity
Score > 22 indicates homology
VAALNGMPGVLSAR + Oxidation (M)
3622 1155.6581 2309.3016 2309.2638 16.4 1 11 0.4 +1Score > 31 indicates identity
Score > 19 indicates homology
MIPVQGVQNQTIAVLGLGRSGR + Oxidation (M)
3528 1127.0607 2252.1068 2252.1188 -5.29 0 11 0.23 +1Score > 39 indicates identity
Score > 17 indicates homology
IFVQGIILQICSFDQWGVE + Deamidated (NQ)
939 467.7264 933.4382 933.4225 16.8 1 11 0.7 +1Score > 42 indicates identity
Score > 22 indicates homology
MANPLRSE + Deamidated (NQ); Oxidation (M)
2874 930.9705 1859.9264 1859.9014 13.5 1 11 0.87 +1Score > 40 indicates identity
Score > 23 indicates homology
APGQDVTLFRSPDSVDR + Deamidated (NQ)
1293 367.2097 1098.6073 1098.5934 12.6 1 11 0.68 +1Score > 40 indicates identity
Score > 22 indicates homology
NGKHQIVFR + Deamidated (NQ)
2880 933.4937 1864.9728 1864.9829 -5.39 1 11 0.64 +1Score > 39 indicates identity
Score > 21 indicates homology
GMKVPDVLLSGNHAAIAR + Deamidated (NQ); Oxidation (M)
958 314.5250 940.5532 940.5705 -18.5 1 11 0.91 +1Score > 38 indicates identity
Score > 23 indicates homology
QVSPAAIKK
1864 683.8204 1365.6262 1365.6234 2.08 1 11 0.94 +1Score > 40 indicates identity
Score > 23 indicates homology
AIMNFINGNNEK + 2 Deamidated (NQ)
3648 775.7334 2324.1784 2324.2046 -11.3 1 11 0.28 +1Score > 39 indicates identity
Score > 18 indicates homology
SVVRVPTSSMSLLDGTYNLLR + Deamidated (NQ); Oxidation (M)
1602 618.3071 1234.5996 1234.5976 1.68 0 11 0.82 +1Score > 42 indicates identity
Score > 22 indicates homology
AGAVTVATNMAGR + Deamidated (NQ); Oxidation (M)
3295 1067.5358 2133.0570 2133.0451 5.62 1 11 0.89 +1Score > 40 indicates identity
Score > 23 indicates homology
TGEGSAVFNGAQNGVSQLIQR + Deamidated (NQ)
3313 712.7119 2135.1139 2135.0833 14.3 1 11 0.14 +1Score > 38 indicates identity
Score > 15 indicates homology
LVHHDIIDALENMSRPFK + Deamidated (NQ)
405 702.3802 701.3729 701.3708 3.02 0 11 0.37 +1Score > 45 indicates identity
Score > 19 indicates homology
TPDTIR
2321 526.2891 1575.8455 1575.8620 -10.5 1 11 0.4 +1Score > 39 indicates identity
Score > 19 indicates homology
SIVFGKDAQALAISR + Deamidated (NQ)
2384 804.8953 1607.7760 1607.8042 -17.5 1 11 0.89 +1Score > 41 indicates identity
Score > 23 indicates homology
NKLANISQDQFLSK + 3 Deamidated (NQ)
3066 996.0188 1990.0230 1990.0081 7.52 1 11 0.93 +1Score > 39 indicates identity
Score > 23 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
2494 830.9236 1659.8326 1659.8000 19.6 0 11 0.47 +1Score > 41 indicates identity
Score > 20 indicates homology
SKPVQMMYQIGSFK + Deamidated (NQ); Oxidation (M)
3332 1074.0675 2146.1204 2146.1494 -13.5 1 11 0.24 +1Score > 38 indicates identity
Score > 17 indicates homology
HNVSNATAAVAVAQRLGIKPE + Deamidated (NQ)
505 375.2254 748.4362 748.4344 2.47 1 11 0.74 +1Score > 39 indicates identity
Score > 22 indicates homology
TVFRAR
2838 919.4421 1836.8696 1836.8927 -12.6 1 11 0.49 +1Score > 40 indicates identity
Score > 20 indicates homology
NMNTSKFLALTPQLDE + Oxidation (M)
2849 615.9737 1844.8993 1844.8734 14.0 0 11 0.88 +1Score > 40 indicates identity
Score > 23 indicates homology
HFFDSQTNFIFVNTK + Deamidated (NQ)
2994 971.5378 1941.0610 1941.0935 -16.7 0 11 0.61 +1Score > 36 indicates identity
Score > 21 indicates homology
LLIVDRPIDQIAPVIHE + Deamidated (NQ)
1724 647.9106 1293.8066 1293.7842 17.3 0 11 1 +1Score > 27 indicates identity
Score > 23 indicates homology
MIQVLGPKPALK
2333 527.5975 1579.7707 1579.7664 2.67 1 11 0.64 +1Score > 41 indicates identity
Score > 21 indicates homology
TWSMVSVLRQGATE + Oxidation (M)
3477 743.0593 2226.1561 2226.1321 10.8 1 11 0.91 +1Score > 38 indicates identity
Score > 23 indicates homology
AIFPGTFDPLTNGHINLIER + 2 Deamidated (NQ)
3420 730.3700 2188.0882 2188.1173 -13.3 1 11 0.16 +1Score > 39 indicates identity
Score > 15 indicates homology
ICNGRYFINTAMILFLNK + Deamidated (NQ)
1246 1072.5715 1071.5642 1071.5448 18.2 1 11 0.35 +1Score > 42 indicates identity
Score > 19 indicates homology
VEQIAAIAQE + Deamidated (NQ)
2136 749.9395 1497.8644 1497.8879 -15.6 1 11 0.81 +1Score > 34 indicates identity
Score > 22 indicates homology
LSIRAGDLVQIVSK
1854 454.2552 1359.7438 1359.7366 5.25 1 11 0.51 +1Score > 39 indicates identity
Score > 20 indicates homology
AAGRIAMLMPGLK + 2 Oxidation (M)
2594 569.6595 1705.9567 1705.9363 12.0 1 11 0.9 +1Score > 35 indicates identity
Score > 23 indicates homology
SPIVEPGLEALLQQGR
2417 808.4220 1614.8294 1614.8154 8.67 1 11 0.71 +1Score > 40 indicates identity
Score > 22 indicates homology
RGGGGAGALLWQVFPE + Deamidated (NQ)
2633 861.9764 1721.9382 1721.9451 -4.00 0 11 0.88 +1Score > 37 indicates identity
Score > 23 indicates homology
DAVTTIISDLLSVTFK
1951 703.3419 1404.6692 1404.6966 -19.4 1 11 0.86 +1Score > 41 indicates identity
Score > 22 indicates homology
GMVGRNLIACAAR + Deamidated (NQ); Oxidation (M)
2014 479.6116 1435.8130 1435.8035 6.62 0 11 0.62 +1Score > 35 indicates identity
Score > 21 indicates homology
LSGVEPLGQPGLLR + Deamidated (NQ)
2180 759.3973 1516.7800 1516.7773 1.80 0 11 0.44 +1Score > 41 indicates identity
Score > 20 indicates homology
YIAQALPQDVLGTE
2542 837.4715 1672.9284 1672.9070 12.8 1 11 0.58 +1Score > 36 indicates identity
Score > 21 indicates homology
EKPTILLMVGVQGSGK + Deamidated (NQ); Oxidation (M)
3921 852.7883 2555.3431 2555.3703 -10.6 1 11 0.11 +1Score > 36 indicates identity
Score > 13 indicates homology
DLAVGITKAMGAAVCGVDLIIPDLK + Oxidation (M)
3803 819.0835 2454.2287 2454.1863 17.3 0 11 0.24 +1Score > 38 indicates identity
Score > 17 indicates homology
TPIHHYSGNNLDLGTACGKPFR
2870 620.3234 1857.9484 1857.9506 -1.19 0 11 0.52 +1Score > 40 indicates identity
Score > 20 indicates homology
LLDLAEPGLSSLMQAQR + Deamidated (NQ); Oxidation (M)
1787 441.9158 1322.7256 1322.7419 -12.3 1 11 0.99 +1Score > 38 indicates identity
Score > 23 indicates homology
ILTNISNRVHR + Deamidated (NQ)
3506 748.7404 2243.1994 2243.2175 -8.08 0 11 0.95 +1Score > 36 indicates identity
Score > 23 indicates homology
AADVAAGGQGAPLVPVFHQALVR
660 817.4210 816.4137 816.4090 5.83 0 11 0.49 +1Score > 43 indicates identity
Score > 20 indicates homology
GNVGSINR + Deamidated (NQ)
2571 566.2950 1695.8632 1695.8576 3.30 0 11 0.18 +1Score > 40 indicates identity
Score > 16 indicates homology
LTFLCVTLNCIDVK + Deamidated (NQ)
1513 600.3367 1198.6588 1198.6418 14.2 1 11 0.8 +1Score > 40 indicates identity
Score > 22 indicates homology
QLLGSGSREPR
3141 680.3470 2038.0192 2038.0485 -14.4 1 11 0.26 +1Score > 40 indicates identity
Score > 17 indicates homology
VNLFTLLSLWMELFPAE + Oxidation (M)
3600 767.7409 2300.2009 2300.1722 12.5 1 11 0.15 +1Score > 37 indicates identity
Score > 15 indicates homology
AIIMPNLQPPVTTVEQAAAYR + 2 Deamidated (NQ); Oxidation (M)
3181 687.3600 2059.0582 2059.0700 -5.73 1 11 0.97 +1Score > 39 indicates identity
Score > 23 indicates homology
AMAIFELLDYIVNEPPPK
1752 437.2230 1308.6472 1308.6271 15.3 0 11 0.99 +1Score > 41 indicates identity
Score > 23 indicates homology
MNLTLNFVDPK + 2 Deamidated (NQ); Oxidation (M)
780 436.2529 870.4912 870.4923 -1.20 1 10 0.82 +1Score > 40 indicates identity
Score > 22 indicates homology
NQLEVLR
3665 780.7187 2339.1343 2339.1604 -11.2 1 10 0.53 +1Score > 39 indicates identity
Score > 20 indicates homology
QNLLQAAGNVGQASGELLQQIGE + 2 Deamidated (NQ)
2249 774.9380 1547.8614 1547.8811 -12.7 0 10 0.91 +1Score > 37 indicates identity
Score > 23 indicates homology
LTAFTAGLLLITVSE
3011 653.3485 1957.0237 1956.9945 14.9 0 10 0.31 +1Score > 38 indicates identity
Score > 18 indicates homology
LGWSQPLGNLQAPLGYDK + Deamidated (NQ)
1121 506.7423 1011.4700 1011.4695 0.53 1 10 0.67 +1Score > 40 indicates identity
Score > 21 indicates homology
MAVDIEYR + Oxidation (M)
1561 609.8657 1217.7168 1217.7132 3.00 1 10 0.64 +1Score > 34 indicates identity
Score > 21 indicates homology
FVGSLNKVNLK
1651 631.3170 1260.6194 1260.6132 4.94 1 10 0.57 +1Score > 41 indicates identity
Score > 21 indicates homology
AQVMGGARPKTE + Deamidated (NQ); Oxidation (M)
892 459.2351 916.4556 916.4502 5.98 1 10 0.99 +1Score > 42 indicates identity
Score > 23 indicates homology
AATLQVEGQ + Deamidated (NQ)
2793 603.6453 1807.9141 1807.9178 -2.09 1 10 0.47 +1Score > 40 indicates identity
Score > 20 indicates homology
VTLYLDWLEMATTPR
3398 727.0785 2178.2137 2178.2008 5.91 1 10 0.15 +1Score > 33 indicates identity
Score > 15 indicates homology
LIQQLQQQALSEPAVVQKR + 2 Deamidated (NQ)
875 455.7576 909.5006 909.4920 9.55 0 10 0.63 +1Score > 38 indicates identity
Score > 21 indicates homology
THLLNALE
1306 369.8681 1106.5825 1106.5641 16.6 1 10 0.45 +1Score > 41 indicates identity
Score > 19 indicates homology
KMNSAVILSE + Oxidation (M)
1126 338.8503 1013.5291 1013.5406 -11.4 1 10 0.67 +1Score > 40 indicates identity
Score > 21 indicates homology
SKGHPAVYR
1377 380.5536 1138.6390 1138.6460 -6.20 1 10 0.57 +1Score > 37 indicates identity
Score > 20 indicates homology
KFVFLMIIE
1733 650.3890 1298.7634 1298.7809 -13.4 1 10 0.81 +1Score > 35 indicates identity
Score > 22 indicates homology
ILTAQALELSLK
1780 331.1999 1320.7705 1320.7765 -4.55 0 10 0.85 +1Score > 33 indicates identity
Score > 22 indicates homology
AIDNLPLSLIPR
1861 456.2155 1365.6247 1365.6347 -7.34 1 10 0.84 +1Score > 40 indicates identity
Score > 22 indicates homology
LLGMDPEHGRPE + Oxidation (M)
2150 376.9364 1503.7165 1503.7317 -10.1 1 10 0.77 +1Score > 41 indicates identity
Score > 22 indicates homology
DAVYNGPQDLAGKR + Deamidated (NQ)
3224 699.0405 2094.0997 2094.0892 5.02 1 10 0.39 +1Score > 38 indicates identity
Score > 19 indicates homology
RAIMLNQPNPQDGVDVLAK + Oxidation (M)
272 316.6930 631.3714 631.3693 3.34 0 10 0.56 +1Score > 41 indicates identity
Score > 20 indicates homology
APGFLK
1398 383.8665 1148.5777 1148.5767 0.83 0 10 0.82 +1Score > 42 indicates identity
Score > 22 indicates homology
DAPFFHFLR
160 506.2679 505.2606 505.2570 7.13 0 10 1 +1Score > 43 indicates identity
Score > 23 indicates homology
MGGLL + Oxidation (M)
1196 521.8009 1041.5872 1041.5818 5.18 0 10 0.84 +1Score > 40 indicates identity
Score > 22 indicates homology
LNPTLDTLR
2666 870.9808 1739.9470 1739.9669 -11.4 1 10 0.99 +1Score > 37 indicates identity
Score > 23 indicates homology
DKIILAIDGLDLNQAK + Deamidated (NQ)
3152 682.0107 2043.0103 2042.9949 7.53 1 10 0.67 +1Score > 39 indicates identity
Score > 21 indicates homology
LFSLLSDFYLNPTNNER + Deamidated (NQ)
1573 613.3505 1224.6864 1224.6866 -0.15 1 10 0.68 +1Score > 37 indicates identity
Score > 21 indicates homology
ISIKNISFFR + Deamidated (NQ)
1989 712.3606 1422.7066 1422.6991 5.34 1 10 0.52 +1Score > 41 indicates identity
Score > 20 indicates homology
VELAAQSYDTIGR + Deamidated (NQ)
3064 996.0168 1990.0190 1990.0081 5.51 1 10 0.56 +1Score > 40 indicates identity
Score > 20 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
887 458.2812 914.5478 914.5549 -7.71 1 10 0.68 +1Score > 40 indicates identity
Score > 21 indicates homology
IGDRLLTK
3056 994.5299 1987.0452 1987.0700 -12.4 1 10 0.77 +1Score > 38 indicates identity
Score > 22 indicates homology
INAAWGMTVLLLDVLTEK + Deamidated (NQ)
673 414.7237 827.4328 827.4177 18.3 0 10 0.97 +1Score > 38 indicates identity
Score > 23 indicates homology
NAYGYLK
1468 391.8612 1172.5618 1172.5536 6.99 0 10 0.89 +1Score > 40 indicates identity
Score > 22 indicates homology
GLYDNGYMIK
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