MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 3956)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3378 1086.1490 2170.2834 2170.2911 -3.54 0 13 0.22 +1Score > 26 indicates identity
Score > 19 indicates homology
LIIFAMNGVLVAIAGLILSAR + Oxidation (M)
1892 346.1690 1380.6469 1380.6443 1.90 1 12 0.34 +1Score > 41 indicates identity
Score > 20 indicates homology
SSVGMVEVNTVDK + Deamidated (NQ); Oxidation (M)
2052 729.9156 1457.8166 1457.7878 19.8 0 12 0.48 +1Score > 38 indicates identity
Score > 22 indicates homology
LTIAQSHFSLVNK + Deamidated (NQ)
2939 478.5010 1909.9749 1909.9858 -5.69 1 12 0.74 +1Score > 39 indicates identity
Score > 24 indicates homology
VTVPNNVRVQNVDLTNE
1700 642.8716 1283.7286 1283.7085 15.7 1 12 0.93 +1Score > 38 indicates identity
Score > 25 indicates homology
LLNQKIDPTNK + Deamidated (NQ)
3062 663.7126 1988.1160 1988.0843 15.9 1 12 0.097 +1Score > 33 indicates identity
Score > 15 indicates homology
IRNITLSQQGHGLIYFK + Deamidated (NQ)
3953 863.4337 2587.2793 2587.2839 -1.80 1 12 0.13 +1Score > 38 indicates identity
Score > 16 indicates homology
YQAQIDQYLGLVRTNMNTIVTK + 3 Deamidated (NQ); Oxidation (M)
217 301.6803 601.3460 601.3548 -14.5 0 12 0.96 +1Score > 45 indicates identity
Score > 25 indicates homology
ISAGVR
995 479.2559 956.4972 956.4927 4.76 0 12 0.63 +1Score > 40 indicates identity
Score > 23 indicates homology
TITAHAQSK + Deamidated (NQ)
1649 630.3449 1258.6752 1258.6517 18.7 0 12 0.49 +1Score > 41 indicates identity
Score > 22 indicates homology
VAGGTVQIDAATR + Deamidated (NQ)
3943 861.4436 2581.3090 2581.3309 -8.50 1 12 0.15 +1Score > 38 indicates identity
Score > 17 indicates homology
IPSAVLNDVVMDAVAINPTPTDKGK + Deamidated (NQ); Oxidation (M)
3574 760.4044 2278.1914 2278.1661 11.1 1 12 0.23 +1Score > 38 indicates identity
Score > 19 indicates homology
MSAIQGIEGVISLLQATAMSAR + 2 Oxidation (M)
1150 510.3023 1018.5900 1018.5811 8.75 0 12 0.79 +1Score > 38 indicates identity
Score > 24 indicates homology
GYITVTPLR
1803 665.3483 1328.6820 1328.6936 -8.69 1 12 0.6 +1Score > 41 indicates identity
Score > 23 indicates homology
QVVVNLLGSRGGE + 2 Deamidated (NQ)
977 474.7647 947.5148 947.4977 18.1 1 12 0.84 +1Score > 41 indicates identity
Score > 24 indicates homology
AAYPGWKR
813 443.7608 885.5070 885.4919 17.1 1 12 0.7 +1Score > 41 indicates identity
Score > 23 indicates homology
LAKNNPTK + Deamidated (NQ)
2832 609.9806 1826.9200 1826.9526 -17.9 0 12 0.84 +1Score > 40 indicates identity
Score > 24 indicates homology
IPTLSQVQALSADWAAR + Deamidated (NQ)
832 447.7761 893.5376 893.5334 4.72 0 12 0.8 +1Score > 33 indicates identity
Score > 24 indicates homology
IVLAPEPR
1450 390.2180 1167.6322 1167.6472 -12.9 1 12 0.84 +1Score > 39 indicates identity
Score > 24 indicates homology
SITRHGITAGR
2441 544.3010 1629.8812 1629.8946 -8.24 0 12 0.87 +1Score > 39 indicates identity
Score > 24 indicates homology
MVLNGANLVTALVMGK
612 398.1989 794.3832 794.3922 -11.3 1 12 0.24 +1Score > 41 indicates identity
Score > 19 indicates homology
YEIQSR
932 930.5062 929.4989 929.4818 18.4 1 12 0.38 +1Score > 42 indicates identity
Score > 21 indicates homology
KDLAINQE
1009 481.2561 960.4976 960.5062 -8.95 1 12 0.92 +1Score > 42 indicates identity
Score > 25 indicates homology
LIGEMVGAR + Oxidation (M)
1022 322.8515 965.5327 965.5195 13.6 0 12 0.77 +1Score > 38 indicates identity
Score > 24 indicates homology
IHTHGVFR
705 421.7234 841.4322 841.4406 -9.92 0 12 0.8 +1Score > 39 indicates identity
Score > 24 indicates homology
NTPNQIR
2196 508.2501 1521.7285 1521.7246 2.55 0 12 0.56 +1Score > 41 indicates identity
Score > 22 indicates homology
ILGFGDSVCDNLGR
2829 913.4863 1824.9580 1824.9767 -10.2 0 12 0.62 +1Score > 39 indicates identity
Score > 23 indicates homology
SGILLSNPVNAIPLMNR + Deamidated (NQ); Oxidation (M)
2074 735.3625 1468.7104 1468.7270 -11.3 1 12 0.43 +1Score > 40 indicates identity
Score > 21 indicates homology
TTKGAPVATNHQSR + 2 Deamidated (NQ)
278 318.1807 634.3468 634.3360 17.1 1 12 1 +1Score > 41 indicates identity
Score > 25 indicates homology
MEIVK + Oxidation (M)
2674 581.6456 1741.9150 1741.9033 6.72 0 12 0.75 +1Score > 39 indicates identity
Score > 24 indicates homology
QNQPQLVIITGMSGAGK + Deamidated (NQ)
3720 1190.6422 2379.2698 2379.2885 -7.86 0 12 0.43 +1Score > 36 indicates identity
Score > 21 indicates homology
FGIGADGVLFLAKPLHTSLHMR
2282 783.4281 1564.8416 1564.8460 -2.80 0 12 0.24 +1Score > 38 indicates identity
Score > 19 indicates homology
TNAQAALLASKPPGPK + 2 Deamidated (NQ)
2410 538.9547 1613.8423 1613.8202 13.7 1 12 0.72 +1Score > 40 indicates identity
Score > 23 indicates homology
WAVQVLQVAEQWR + 2 Deamidated (NQ)
2956 641.6863 1922.0371 1922.0338 1.73 1 12 0.54 +1Score > 37 indicates identity
Score > 22 indicates homology
MTRCALLLLMVLMLGR + 2 Oxidation (M)
3260 1059.0383 2116.0620 2116.0973 -16.7 0 12 0.99 +1Score > 39 indicates identity
Score > 25 indicates homology
TVTVLNTILLQNMGAALTPE + 2 Deamidated (NQ); Oxidation (M)
1815 668.8309 1335.6472 1335.6340 9.91 1 12 0.82 +1Score > 41 indicates identity
Score > 24 indicates homology
VVQMTNDDIKR + 2 Deamidated (NQ); Oxidation (M)
3440 550.2770 2197.0789 2197.0434 16.2 0 12 0.69 +1Score > 39 indicates identity
Score > 23 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
1865 683.8206 1365.6266 1365.6234 2.37 1 12 0.69 +1Score > 40 indicates identity
Score > 23 indicates homology
AIMNFINGNNEK + 2 Deamidated (NQ)
421 354.6888 707.3630 707.3636 -0.80 0 12 0.35 +1Score > 43 indicates identity
Score > 20 indicates homology
MSVTVR + Oxidation (M)
367 342.2271 682.4396 682.4378 2.77 0 12 0.79 +1Score > 34 indicates identity
Score > 24 indicates homology
VPAVIGK
1453 585.8279 1169.6412 1169.6404 0.73 1 12 0.7 +1Score > 40 indicates identity
Score > 23 indicates homology
QIVNRINALE + Deamidated (NQ)
2488 551.9777 1652.9113 1652.8985 7.75 1 12 0.44 +1Score > 37 indicates identity
Score > 21 indicates homology
IPQINDDILTIAEAK
3866 837.4678 2509.3816 2509.3462 14.1 1 12 0.41 +1Score > 33 indicates identity
Score > 21 indicates homology
VAEMLAPVLTGNDLILVQGAGNIGK + Deamidated (NQ); Oxidation (M)
4087 910.4649 2728.3729 2728.3386 12.5 1 12 0.95 +1Score > 37 indicates identity
Score > 25 indicates homology
RVLAYSTMSQLGYMMLALGIGSYR + 3 Oxidation (M)
1900 693.8630 1385.7114 1385.6973 10.2 0 12 0.82 +1Score > 41 indicates identity
Score > 24 indicates homology
MAPALSGANLTSPR + Deamidated (NQ)
2543 837.4736 1672.9326 1672.9070 15.4 1 12 0.84 +1Score > 36 indicates identity
Score > 24 indicates homology
EKPTILLMVGVQGSGK + Deamidated (NQ); Oxidation (M)
3010 653.0266 1956.0580 1956.0316 13.5 1 12 0.52 +1Score > 37 indicates identity
Score > 22 indicates homology
RYIPQAIAQGVAAVVAQAE + 2 Deamidated (NQ)
2392 806.4384 1610.8622 1610.8926 -18.9 1 12 0.6 +1Score > 39 indicates identity
Score > 23 indicates homology
MAPQLVGAVLIGRDR + Oxidation (M)
1519 601.8319 1201.6492 1201.6554 -5.12 1 12 0.82 +1Score > 41 indicates identity
Score > 24 indicates homology
QGDGLLTKLQK + 2 Deamidated (NQ)
3382 724.7293 2171.1661 2171.1837 -8.14 1 12 0.69 +1Score > 36 indicates identity
Score > 23 indicates homology
LGYTADASALAPGLILQRVIE + Deamidated (NQ)
2651 866.8898 1731.7650 1731.7807 -9.06 0 12 0.67 +1Score > 39 indicates identity
Score > 23 indicates homology
NDNPLVDMQAMSPTAK + Deamidated (NQ)
1316 557.3235 1112.6324 1112.6302 2.03 1 12 0.41 +1Score > 38 indicates identity
Score > 21 indicates homology
SIVPISRGQR + Deamidated (NQ)
1797 664.3441 1326.6736 1326.6714 1.66 0 12 0.73 +1Score > 40 indicates identity
Score > 23 indicates homology
DVHAVVMVGGSTR
3910 638.8063 2551.1961 2551.2377 -16.3 0 12 0.12 +1Score > 38 indicates identity
Score > 16 indicates homology
DMGWTLQTLNHSLGGDVSLHQIK + 2 Deamidated (NQ)
3069 665.0223 1992.0451 1992.0172 14.0 1 12 0.29 +1Score > 38 indicates identity
Score > 19 indicates homology
RMLQAVDGYPMNIGLLGK + Deamidated (NQ); Oxidation (M)
4293 966.5203 2896.5391 2896.4930 15.9 1 12 0.11 +1Score > 34 indicates identity
Score > 15 indicates homology
AFTVPSVELSSAKPSQTSHLPTSLAADR
4129 925.5062 2773.4968 2773.4659 11.1 1 12 0.18 +1Score > 33 indicates identity
Score > 17 indicates homology
RGNVMGIALGGLAMGVLVGPPFGSVLYE
1082 499.2918 996.5690 996.5716 -2.57 1 12 0.77 +1Score > 38 indicates identity
Score > 23 indicates homology
LPDQKQLR
1876 685.3387 1368.6628 1368.6786 -11.5 1 12 0.59 +1Score > 40 indicates identity
Score > 22 indicates homology
IVAVDAESSHWR
3034 658.3710 1972.0912 1972.1132 -11.2 1 12 0.12 +1Score > 35 indicates identity
Score > 15 indicates homology
IILVILDAISNIFQAAEK + 2 Deamidated (NQ)
171 530.3309 529.3236 529.3224 2.37 0 12 0.58 +1Score > 47 indicates identity
Score > 22 indicates homology
AAAIGK
1045 490.7657 979.5168 979.5161 0.78 1 12 0.93 +1Score > 41 indicates identity
Score > 24 indicates homology
IVGEMFIR + Oxidation (M)
1304 554.2531 1106.4916 1106.4914 0.26 1 12 0.77 +1Score > 40 indicates identity
Score > 23 indicates homology
VLDQMNENK + Deamidated (NQ); Oxidation (M)
2565 846.4707 1690.9268 1690.9328 -3.49 0 12 0.84 +1Score > 36 indicates identity
Score > 24 indicates homology
MDIINIPTYLTLLR + Oxidation (M)
2087 738.4008 1474.7870 1474.7780 6.17 0 12 0.71 +1Score > 40 indicates identity
Score > 23 indicates homology
ANNAILQFLDISR + Deamidated (NQ)
1195 521.7799 1041.5452 1041.5455 -0.20 0 12 0.88 +1Score > 39 indicates identity
Score > 24 indicates homology
VNPNVQLTR + 2 Deamidated (NQ)
1336 374.5501 1120.6285 1120.6241 3.93 0 12 0.98 +1Score > 38 indicates identity
Score > 24 indicates homology
SGVTITAGLFR
1955 705.3646 1408.7146 1408.7054 6.57 1 12 0.81 +1Score > 41 indicates identity
Score > 24 indicates homology
QKMSVLNMVVSR + 2 Deamidated (NQ); Oxidation (M)
2360 796.8975 1591.7804 1591.7617 11.8 1 12 0.96 +1Score > 41 indicates identity
Score > 24 indicates homology
LAAEDPSLVFTVDSE
1448 584.8218 1167.6290 1167.6499 -17.9 1 12 1 +1Score > 39 indicates identity
Score > 24 indicates homology
VAGLLNKPVEE
1845 677.8801 1353.7456 1353.7616 -11.8 1 12 0.95 +1Score > 38 indicates identity
Score > 24 indicates homology
VLENLLQVSPSR
3685 786.4272 2356.2598 2356.2195 17.1 1 12 0.19 +1Score > 36 indicates identity
Score > 17 indicates homology
LPIIALLATQAQGTTCIKDAQE + 2 Deamidated (NQ)
2232 514.6288 1540.8646 1540.8460 12.0 1 12 0.91 +1Score > 37 indicates identity
Score > 24 indicates homology
AAEAILIALGQLNSR + 2 Deamidated (NQ)
2473 549.2975 1644.8707 1644.8736 -1.80 1 12 0.61 +1Score > 39 indicates identity
Score > 22 indicates homology
RPLSWVVGEQGVYR
742 428.7309 855.4472 855.4385 10.2 0 12 0.93 +1Score > 39 indicates identity
Score > 24 indicates homology
VHQMLGR + Oxidation (M)
3246 1056.0518 2110.0890 2110.0946 -2.64 1 12 0.32 +1Score > 38 indicates identity
Score > 20 indicates homology
AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
2544 558.6520 1672.9342 1672.9261 4.85 1 12 0.59 +1Score > 36 indicates identity
Score > 22 indicates homology
VKTSVGVWGALLNSSR
2240 515.5795 1543.7167 1543.7114 3.42 1 12 0.73 +1Score > 39 indicates identity
Score > 23 indicates homology
NGLVNGENSIPNGTR + 3 Deamidated (NQ)
2373 533.6324 1597.8754 1597.8675 4.90 0 12 0.31 +1Score > 38 indicates identity
Score > 19 indicates homology
TVADLGSGNGSPGIILK
1113 337.5099 1009.5079 1009.5192 -11.3 1 12 0.7 +1Score > 40 indicates identity
Score > 23 indicates homology
NVSSNFSKK
4001 882.8120 2645.4142 2645.3961 6.82 0 12 0.24 +1Score > 35 indicates identity
Score > 18 indicates homology
GKPMGIALGGLAMGVLVGPPFGSVLYE + Oxidation (M)
4131 925.5106 2773.5100 2773.4659 15.9 1 12 0.13 +1Score > 33 indicates identity
Score > 16 indicates homology
RGNVMGIALGGLAMGVLVGPPFGSVLYE
1990 712.3940 1422.7734 1422.7871 -9.59 1 12 0.66 +1Score > 38 indicates identity
Score > 23 indicates homology
FVSFKPSTLEIR
2620 860.4751 1718.9356 1718.9277 4.65 1 12 0.38 +1Score > 37 indicates identity
Score > 20 indicates homology
ISVFKGAPNSMINLVK + 2 Deamidated (NQ)
1188 518.2980 1034.5814 1034.5760 5.23 1 12 0.98 +1Score > 38 indicates identity
Score > 24 indicates homology
LALEVFTSR
1447 390.2168 1167.6286 1167.6472 -16.0 1 12 0.83 +1Score > 39 indicates identity
Score > 24 indicates homology
SITRHGITAGR
2323 527.2555 1578.7447 1578.7638 -12.1 1 12 0.72 +1Score > 41 indicates identity
Score > 23 indicates homology
RFSNLSNSAGNAVLE + Deamidated (NQ)
1810 445.2660 1332.7762 1332.7918 -11.7 0 12 0.48 +1Score > 34 indicates identity
Score > 21 indicates homology
AIFINIFGGILR
2030 722.8690 1443.7234 1443.7205 2.03 1 12 0.39 +1Score > 41 indicates identity
Score > 20 indicates homology
APTTSDVSKPKANE
2628 861.4428 1720.8710 1720.8420 16.9 1 12 0.66 +1Score > 40 indicates identity
Score > 22 indicates homology
ELVLNNVYSNVWNR + 2 Deamidated (NQ)
3324 1071.5735 2141.1324 2141.1255 3.23 1 12 0.85 +1Score > 37 indicates identity
Score > 24 indicates homology
TLNLLLSYLNYSVDNIRK + 3 Deamidated (NQ)
3281 708.0740 2121.2002 2121.1649 16.6 0 12 0.11 +1Score > 32 indicates identity
Score > 15 indicates homology
IDFIQVIIYIGFPNLLIE + 2 Deamidated (NQ)
223 301.6983 601.3820 601.3799 3.55 0 12 0.9 +1Score > 41 indicates identity
Score > 24 indicates homology
LVSVGK
1703 643.8627 1285.7108 1285.7241 -10.3 1 12 0.7 +1Score > 40 indicates identity
Score > 23 indicates homology
LADNQTKLAALK + Deamidated (NQ)
2881 933.9279 1865.8412 1865.8499 -4.64 0 12 0.76 +1Score > 39 indicates identity
Score > 23 indicates homology
LDLMDQGTNAMDILSGR + Deamidated (NQ); Oxidation (M)
2911 629.6773 1886.0101 1885.9938 8.65 1 12 0.89 +1Score > 38 indicates identity
Score > 24 indicates homology
ASAKTVNVILPYYGYAR + Deamidated (NQ)
2298 524.6119 1570.8139 1570.8427 -18.4 1 12 0.97 +1Score > 40 indicates identity
Score > 24 indicates homology
LVGTTNQGDQRILR + Deamidated (NQ)
365 341.6986 681.3826 681.3810 2.47 0 12 0.97 +1Score > 37 indicates identity
Score > 24 indicates homology
IDVAHK
1603 618.3169 1234.6192 1234.6306 -9.18 0 12 0.59 +1Score > 41 indicates identity
Score > 22 indicates homology
SQPPTHAQGIAK + Deamidated (NQ)
2855 926.4800 1850.9454 1850.9237 11.8 1 12 0.82 +1Score > 39 indicates identity
Score > 23 indicates homology
EMNPTHIIISPGPGFPK + Deamidated (NQ); Oxidation (M)
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