MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2552 421.7231 1682.8633 1682.8661 -1.67 1 13 0.72 +1Score > 40 indicates identity
Score > 25 indicates homology		 GMHSLQNALLSELLR + 2 Deamidated (NQ)
2334 790.8932 1579.7718 1579.7730 -0.72 0 13 0.71 +1Score > 41 indicates identity
Score > 24 indicates homology		 YTVQNLTVQAVDTE
2502 555.9804 1664.9194 1664.9025 10.1 1 13 0.34 +1Score > 36 indicates identity
Score > 21 indicates homology		 FGYINLQDILKIVE + Deamidated (NQ)
162 512.2565 511.2492 511.2391 19.9 0 13 1 +1Score > 40 indicates identity
Score > 26 indicates homology		 GHDAL
1319 558.3054 1114.5962 1114.5982 -1.78 0 13 0.69 +1Score > 41 indicates identity
Score > 24 indicates homology		 ALNIDVTVGGR + Deamidated (NQ)
3834 826.4409 2476.3009 2476.2640 14.9 1 13 0.24 +1Score > 36 indicates identity
Score > 20 indicates homology		 HLNTMGKTIIIITHDMHLMLE + Oxidation (M)
2863 619.3448 1855.0126 1855.0488 -19.5 1 13 0.25 +1Score > 37 indicates identity
Score > 20 indicates homology		 LLLLDEPLSALDLKMR + Oxidation (M)
2520 556.9583 1667.8531 1667.8618 -5.21 1 13 0.2 +1Score > 40 indicates identity
Score > 19 indicates homology		 ITATSVPPIINIEGNE + Deamidated (NQ)
694 419.6910 837.3674 837.3725 -5.98 0 13 0.85 +1Score > 40 indicates identity
Score > 25 indicates homology		 QMDAVMK + Oxidation (M)
1729 649.3484 1296.6822 1296.6898 -5.84 1 13 0.13 +1Score > 40 indicates identity
Score > 17 indicates homology		 NVTPNNNIRQK
2361 796.8980 1591.7814 1591.7617 12.4 1 13 0.86 +1Score > 41 indicates identity
Score > 25 indicates homology		 LAAEDPSLVFTVDSE
1547 607.8567 1213.6988 1213.7030 -3.44 0 13 0.31 +1Score > 38 indicates identity
Score > 21 indicates homology		 QSIAIGQGILSK
1970 708.4034 1414.7922 1414.7933 -0.73 1 13 0.2 +1Score > 38 indicates identity
Score > 19 indicates homology		 IVVTRDPDVVFR
1259 541.8066 1081.5986 1081.6066 -7.38 1 13 0.57 +1Score > 37 indicates identity
Score > 23 indicates homology		 APGVGPKVAMR
2064 488.2868 1461.8386 1461.8443 -3.89 1 13 0.55 +1Score > 34 indicates identity
Score > 23 indicates homology		 ATPPPSPLLSELLK
1055 495.2828 988.5510 988.5593 -8.36 1 13 0.55 +1Score > 40 indicates identity
Score > 23 indicates homology		 LYGLPKAVE
155 500.2854 499.2781 499.2754 5.38 1 13 0.88 +1Score > 39 indicates identity
Score > 25 indicates homology		 GKAQP
1937 701.3827 1400.7508 1400.7623 -8.20 1 13 0.31 +1Score > 40 indicates identity
Score > 21 indicates homology		 SVTQVNRDLLAGK + Deamidated (NQ)
3250 705.3827 2113.1263 2113.1089 8.22 1 13 0.69 +1Score > 37 indicates identity
Score > 24 indicates homology		 MSVLLGQILDALGGELVGGDR + Deamidated (NQ)
1433 387.8892 1160.6458 1160.6401 4.91 1 13 0.91 +1Score > 39 indicates identity
Score > 25 indicates homology		 SSLSESAILVR
2233 514.9570 1541.8492 1541.8375 7.58 1 13 0.39 +1Score > 38 indicates identity
Score > 22 indicates homology		 ITPIVDMLQGVVEK + Deamidated (NQ)
2481 550.9628 1649.8666 1649.8373 17.8 1 13 0.9 +1Score > 39 indicates identity
Score > 25 indicates homology		 LAELAQLQDQLDHR + Deamidated (NQ)
581 393.2498 784.4850 784.4807 5.57 0 13 0.99 +1Score > 40 indicates identity
Score > 26 indicates homology		 GVLQVIR + Deamidated (NQ)
2118 498.5777 1492.7113 1492.6901 14.2 1 13 0.38 +1Score > 41 indicates identity
Score > 22 indicates homology		 GADVVMMLRLQNE + 2 Deamidated (NQ); Oxidation (M)
2682 871.9958 1741.9770 1741.9475 16.9 0 13 0.83 +1Score > 35 indicates identity
Score > 25 indicates homology		 QLVQQGLGIFGGAIADR
1650 421.2137 1260.6193 1260.6384 -15.1 1 13 0.52 +1Score > 41 indicates identity
Score > 23 indicates homology		 IIQLNEDLMR + Deamidated (NQ); Oxidation (M)
3094 1006.5261 2011.0376 2011.0473 -4.82 1 13 0.84 +1Score > 39 indicates identity
Score > 25 indicates homology		 GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ)
142 487.2664 486.2591 486.2550 8.41 0 13 0.68 +1Score > 32 indicates identity
Score > 24 indicates homology		 SAGPR
249 619.4213 618.4140 618.4105 5.74 0 13 0.18 +1Score > 28 indicates identity
Score > 18 indicates homology		 FIVIK
575 390.1918 778.3690 778.3821 -16.8 0 13 0.58 +1Score > 40 indicates identity
Score > 23 indicates homology		 SSGSGVASK
1048 984.5113 983.5040 983.5036 0.45 0 13 1 +1Score > 40 indicates identity
Score > 26 indicates homology		 RPIVQNQE + Deamidated (NQ)
2332 790.8926 1579.7706 1579.7729 -1.46 1 13 0.81 +1Score > 41 indicates identity
Score > 25 indicates homology		 AQYDTVLANEVTVR + 2 Deamidated (NQ)
740 428.2366 854.4586 854.4538 5.68 0 13 0.77 +1Score > 38 indicates identity
Score > 25 indicates homology		 LGFDYLK
940 467.7301 933.4456 933.4291 17.7 0 13 0.65 +1Score > 42 indicates identity
Score > 24 indicates homology		 LTSADDALE
2985 484.2694 1933.0485 1933.0493 -0.43 1 13 0.61 +1Score > 36 indicates identity
Score > 24 indicates homology		 SAHRSSLGNVLQADLPIR
959 314.8370 941.4892 941.4971 -8.37 1 13 0.41 +1Score > 40 indicates identity
Score > 22 indicates homology		 YKDIFTR
2453 545.3002 1632.8788 1632.8570 13.3 1 13 0.33 +1Score > 38 indicates identity
Score > 21 indicates homology		 VKITLQDNTLTTQR + 3 Deamidated (NQ)
1263 362.5463 1084.6171 1084.5989 16.7 1 13 0.6 +1Score > 39 indicates identity
Score > 23 indicates homology		 TDAPAVTRVR
1551 608.8146 1215.6146 1215.5983 13.5 1 13 0.91 +1Score > 41 indicates identity
Score > 25 indicates homology		 ESLATLNSVPGE
2251 517.2744 1548.8014 1548.7923 5.88 1 13 0.73 +1Score > 40 indicates identity
Score > 24 indicates homology		 VASLELQGNVIYLE + 2 Deamidated (NQ)
3585 763.4354 2287.2844 2287.2827 0.71 0 13 0.36 +1Score > 32 indicates identity
Score > 21 indicates homology		 VLFLQGGASLQFSAIPLNLLGK + 2 Deamidated (NQ)
2376 534.2998 1599.8776 1599.8872 -6.00 1 13 0.58 +1Score > 38 indicates identity
Score > 23 indicates homology		 LPTLFAEQLQLAQK + Deamidated (NQ)
513 377.6810 753.3474 753.3327 19.6 1 13 0.79 +1Score > 39 indicates identity
Score > 25 indicates homology		 TETTMR + Oxidation (M)
3594 767.0899 2298.2479 2298.2505 -1.13 0 13 0.88 +1Score > 35 indicates identity
Score > 25 indicates homology		 IPQVLNGLGIAIVSTSQGIMTGK + 2 Deamidated (NQ)
2650 866.8897 1731.7648 1731.7807 -9.18 0 13 0.72 +1Score > 39 indicates identity
Score > 24 indicates homology		 NDNPLVDMQAMSPTAK + Deamidated (NQ)
637 403.2323 804.4500 804.4606 -13.1 1 13 0.73 +1Score > 41 indicates identity
Score > 24 indicates homology		 FKAGNLR
497 747.4805 746.4732 746.4803 -9.44 1 13 0.41 +1Score > 32 indicates identity
Score > 22 indicates homology		 KFLAIR
1825 671.9150 1341.8154 1341.8020 10.0 1 13 0.19 +1Score > 30 indicates identity
Score > 18 indicates homology		 KVIDYLLTHIK
3006 651.3283 1950.9631 1950.9660 -1.50 1 13 0.09 +1Score > 40 indicates identity
Score > 15 indicates homology		 SNRHIYAQVIDDVHQR + Deamidated (NQ)
2141 751.4091 1500.8036 1500.7784 16.9 1 13 0.33 +1Score > 39 indicates identity
Score > 21 indicates homology		 DLTINAIAQDKNGK + Deamidated (NQ)
2644 576.9510 1727.8312 1727.8440 -7.43 0 13 0.59 +1Score > 40 indicates identity
Score > 23 indicates homology		 DPVVPLNMALPQAAFE + Deamidated (NQ); Oxidation (M)
3310 1068.5621 2135.1096 2135.0858 11.2 1 13 0.5 +1Score > 38 indicates identity
Score > 22 indicates homology		 ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
2848 923.4510 1844.8874 1844.8839 1.91 1 13 0.33 +1Score > 40 indicates identity
Score > 21 indicates homology		 SHVTLPQIRGMFNGDR + 2 Deamidated (NQ); Oxidation (M)
2946 639.0003 1913.9791 1913.9496 15.4 1 13 0.85 +1Score > 39 indicates identity
Score > 25 indicates homology		 YDLAIHAYNKNNSHVR
265 315.2039 628.3932 628.4020 -14.0 1 13 0.9 +1Score > 42 indicates identity
Score > 25 indicates homology		 IGGVKR
2299 786.4144 1570.8142 1570.8090 3.36 0 13 0.98 +1Score > 40 indicates identity
Score > 25 indicates homology		 LNIIQQAALDDQVK + 3 Deamidated (NQ)
3257 706.0630 2115.1672 2115.1398 12.9 1 13 0.51 +1Score > 34 indicates identity
Score > 23 indicates homology		 MPNTNPLFIDALASVVLKR + Deamidated (NQ); Oxidation (M)
2465 547.2997 1638.8773 1638.8729 2.64 1 13 0.26 +1Score > 38 indicates identity
Score > 20 indicates homology		 LDGALASVVPWLNER
743 428.7310 855.4474 855.4450 2.85 0 13 0.94 +1Score > 39 indicates identity
Score > 25 indicates homology		 ISLNSGHK + Deamidated (NQ)
2284 783.9017 1565.7888 1565.8175 -18.3 1 13 0.91 +1Score > 40 indicates identity
Score > 25 indicates homology		 LWAADRAGGPGRPSR
2809 605.9954 1814.9644 1814.9890 -13.6 1 13 0.26 +1Score > 38 indicates identity
Score > 19 indicates homology		 TKVDALAIAIGTSHGAYK
2919 945.0156 1888.0166 1887.9942 11.9 1 13 0.14 +1Score > 37 indicates identity
Score > 17 indicates homology		 EVDAVFVNAILTAIQQR + 2 Deamidated (NQ)
1375 380.5532 1138.6378 1138.6168 18.4 1 13 0.84 +1Score > 37 indicates identity
Score > 25 indicates homology		 LYNMLTRTK
395 349.1722 696.3298 696.3298 -0.0014 1 13 0.88 +1Score > 37 indicates identity
Score > 25 indicates homology		 GKMSMK + Oxidation (M)
1958 705.4045 1408.7944 1408.8038 -6.63 1 13 0.82 +1Score > 36 indicates identity
Score > 25 indicates homology		 KVILDLLNNDPR
514 377.7520 753.4894 753.4861 4.46 1 13 0.59 +1Score > 32 indicates identity
Score > 23 indicates homology		 GLPKIAR
1979 711.3594 1420.7042 1420.6769 19.3 1 13 0.79 +1Score > 41 indicates identity
Score > 24 indicates homology		 NDPYRMVQQLR + 2 Deamidated (NQ)
1981 474.5768 1420.7086 1420.6809 19.5 1 13 0.63 +1Score > 41 indicates identity
Score > 23 indicates homology		 YWPSIPGMGEIR + Oxidation (M)
2679 581.6494 1741.9264 1741.9502 -13.7 1 13 0.59 +1Score > 39 indicates identity
Score > 23 indicates homology		 VPQSVLPDTVFEAIVK + Deamidated (NQ)
3278 1061.5223 2121.0300 2121.0300 0.030 1 13 0.15 +1Score > 39 indicates identity
Score > 17 indicates homology		 QVIYQEANPTGDVTIGMVGK + 2 Deamidated (NQ)
3811 822.1136 2463.3190 2463.3009 7.34 0 13 0.23 +1Score > 35 indicates identity
Score > 19 indicates homology		 IAVAHYAAQLSTLDINPQPLSLE
426 356.7233 711.4320 711.4391 -9.96 1 13 0.67 +1Score > 34 indicates identity
Score > 24 indicates homology		 ARAGLPK
2009 359.6994 1434.7685 1434.7653 2.22 0 13 0.86 +1Score > 39 indicates identity
Score > 25 indicates homology		 VYLNNGIGVMALR + Oxidation (M)
3717 595.7875 2379.1209 2379.1628 -17.6 1 13 0.65 +1Score > 38 indicates identity
Score > 23 indicates homology		 SAVLAQMPSNFIISANVSLDER + 2 Deamidated (NQ); Oxidation (M)
1315 557.3190 1112.6234 1112.6077 14.1 1 13 0.47 +1Score > 40 indicates identity
Score > 22 indicates homology		 LSPIAEQLDK
2246 774.8892 1547.7638 1547.7831 -12.5 1 13 0.57 +1Score > 41 indicates identity
Score > 23 indicates homology		 NVVEVTFNDLAAQK + Deamidated (NQ)
3729 1193.1002 2384.1858 2384.1545 13.2 1 13 0.36 +1Score > 39 indicates identity
Score > 21 indicates homology		 GWELMWLSTGLFACSQQLLK + Deamidated (NQ); Oxidation (M)
1918 464.2603 1389.7591 1389.7755 -11.8 1 13 0.71 +1Score > 39 indicates identity
Score > 24 indicates homology		 ANLLYLLQEAIK + 2 Deamidated (NQ)
1476 393.8892 1178.6458 1178.6547 -7.56 1 13 0.72 +1Score > 38 indicates identity
Score > 24 indicates homology		 GTAIYSELVVK
1077 498.2639 994.5132 994.4940 19.4 0 13 0.96 +1Score > 40 indicates identity
Score > 25 indicates homology		 SLTAVAMMR + Oxidation (M)
1451 584.8413 1167.6680 1167.6612 5.90 1 13 0.7 +1Score > 37 indicates identity
Score > 24 indicates homology		 KGQNPVVLQGK + Deamidated (NQ)
3697 787.4235 2359.2487 2359.2212 11.6 0 13 0.28 +1Score > 36 indicates identity
Score > 20 indicates homology		 SLYAAVTNFLLSLGYHLNPHK + 2 Deamidated (NQ)
2362 531.6157 1591.8253 1591.8028 14.1 1 13 0.89 +1Score > 40 indicates identity
Score > 25 indicates homology		 GVMVKNPNSVYSPGK + Oxidation (M)
2103 740.3547 1478.6948 1478.7226 -18.8 0 13 0.53 +1Score > 41 indicates identity
Score > 22 indicates homology		 AQQIVSTPGHNGNR + Deamidated (NQ)
1685 320.1833 1276.7041 1276.7000 3.21 0 13 0.28 +1Score > 38 indicates identity
Score > 20 indicates homology		 IDIHRPAQTAR
2268 779.8890 1557.7634 1557.7674 -2.56 1 13 0.92 +1Score > 40 indicates identity
Score > 25 indicates homology		 TNTIPNWIKLNNE + 2 Deamidated (NQ)
2406 806.9528 1611.8910 1611.8944 -2.09 0 13 0.28 +1Score > 37 indicates identity
Score > 20 indicates homology		 DGGISGTVIAIAGVLNR
2097 738.8922 1475.7698 1475.7905 -14.0 0 13 0.3 +1Score > 41 indicates identity
Score > 20 indicates homology		 IPQSITSTLGIMSL + Oxidation (M)
1739 651.8636 1301.7126 1301.7231 -8.02 1 13 0.29 +1Score > 40 indicates identity
Score > 20 indicates homology		 FKQLSVGPLLAE + Deamidated (NQ)
2577 567.6461 1699.9165 1699.9104 3.55 1 13 0.7 +1Score > 38 indicates identity
Score > 24 indicates homology		 VANSISDGIKNLDNLK
3637 774.0636 2319.1690 2319.1820 -5.62 1 13 0.81 +1Score > 39 indicates identity
Score > 24 indicates homology		 YYLNGLSTSLPYAAQIKMLR + 2 Deamidated (NQ); Oxidation (M)
2882 622.9665 1865.8777 1865.8782 -0.28 1 13 0.39 +1Score > 40 indicates identity
Score > 21 indicates homology		 VEQQAIFSDQDTLQLK + 4 Deamidated (NQ)
994 319.8396 956.4970 956.5040 -7.30 0 13 0.85 +1Score > 40 indicates identity
Score > 24 indicates homology		 GVNGVGIDAR
2714 880.4557 1758.8968 1758.8934 1.97 1 13 0.87 +1Score > 40 indicates identity
Score > 24 indicates homology		 QLQLQQQRMQLAQK + 3 Deamidated (NQ); Oxidation (M)
3640 774.4196 2320.2370 2320.1924 19.2 1 13 0.26 +1Score > 36 indicates identity
Score > 19 indicates homology		 NFTRITSPNYRPTQQDILR + Deamidated (NQ)
159 506.2669 505.2596 505.2496 19.8 0 13 0.44 +1Score > 44 indicates identity
Score > 22 indicates homology		 SSAGGK
2068 733.4253 1464.8360 1464.8075 19.5 1 13 0.47 +1Score > 34 indicates identity
Score > 22 indicates homology		 QGLLQPENPLLLK + 3 Deamidated (NQ)
1546 405.5645 1213.6717 1213.6489 18.8 0 13 0.9 +1Score > 40 indicates identity
Score > 25 indicates homology		 HLATMAATLAAK + Oxidation (M)
450 364.2177 726.4208 726.4276 -9.29 0 13 0.49 +1Score > 37 indicates identity
Score > 22 indicates homology		 GPVLVDK
3247 704.3706 2110.0900 2110.0484 19.7 0 13 0.62 +1Score > 38 indicates identity
Score > 23 indicates homology		 HLWLDDNALTDVPVQAFR + Deamidated (NQ)
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