MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 3956)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
212 601.3690 600.3617 600.3595 3.68 0 44 0.057 +1Score > 44 indicates identity AGAGVVK
753 430.2552 858.4958 858.4811 17.2 1 41 0.067 +1Score > 42 indicates identity DGAQKIVK + Deamidated (NQ)
465 366.2076 730.4006 730.3973 4.53 0 40 0.15 +1Score > 45 indicates identity VALQSGR + Deamidated (NQ)
749 429.7578 857.5010 857.4858 17.8 1 40 0.093 +1Score > 42 indicates identity QQLVIEK + Deamidated (NQ)
1093 501.2873 1000.5600 1000.5553 4.76 1 40 0.077 +1Score > 41 indicates identity QATLEQALK
756 430.7231 859.4316 859.4287 3.43 1 39 0.14 +1Score > 43 indicates identity
Score > 43 indicates homology		 ILQEGDGK + Deamidated (NQ)
559 773.4734 772.4661 772.4807 -18.8 0 39 0.068 +1Score > 40 indicates identity SSIVLVR
318 658.4163 657.4090 657.4061 4.43 0 39 0.09 +1Score > 41 indicates identity DIVAIK
644 808.4236 807.4163 807.4126 4.56 0 39 0.1 +1Score > 42 indicates identity YDQLAAK
461 365.2352 728.4558 728.4545 1.92 0 39 0.1 +1Score > 42 indicates identity IAVSAIR
299 322.7094 643.4042 643.4017 3.96 0 39 0.11 +1Score > 42 indicates identity AVVSIR
1400 575.3012 1148.5878 1148.5747 11.5 1 39 0.1 +1Score > 42 indicates identity KQIQQMVQK + 3 Deamidated (NQ); Oxidation (M)
534 761.4082 760.4009 760.3967 5.62 1 38 0.18 +1Score > 44 indicates identity ETAQAIK + Deamidated (NQ)
810 443.2460 884.4774 884.4716 6.64 0 38 0.071 +1Score > 39 indicates identity GPQAANVTK
519 757.4594 756.4521 756.4494 3.64 1 38 0.12 +1Score > 41 indicates identity GAVLEIR
674 414.7522 827.4898 827.4865 4.07 0 38 0.081 +1Score > 41 indicates identity
Score > 40 indicates homology		 VQIAQLR + Deamidated (NQ)
531 760.4612 759.4539 759.4490 6.44 0 38 0.056 +1Score > 38 indicates identity QITLASK
560 387.2407 772.4668 772.4629 5.08 0 37 0.11 +1Score > 40 indicates identity CIVILR
562 387.2413 772.4680 772.4629 6.64 0 37 0.11 +1Score > 40 indicates identity CIVILR
3482 744.3647 2230.0723 2230.0430 13.1 1 37 0.094 +1Score > 39 indicates identity ADIDYNTYEAHTTYGVIGVK + Deamidated (NQ)
1268 543.8224 1085.6302 1085.6233 6.37 0 37 0.072 +1Score > 38 indicates identity IQGIGAGFVPK
659 408.7472 815.4798 815.4752 5.66 1 37 0.17 +1Score > 42 indicates identity NEILSLK
415 352.2050 702.3954 702.3912 6.06 0 37 0.18 +1Score > 43 indicates identity
Score > 42 indicates homology		 VTANLGK + Deamidated (NQ)
2206 764.9081 1527.8016 1527.7933 5.48 1 36 0.13 +1Score > 40 indicates identity SEALIQQWVPSLR + 2 Deamidated (NQ)
1090 500.7944 999.5742 999.5713 2.99 0 36 0.092 +1Score > 40 indicates identity
Score > 39 indicates homology		 SAADIIALAR
1269 363.1980 1086.5722 1086.5669 4.83 0 36 0.21 +1Score > 42 indicates identity ITGEPSLGASR
893 459.2468 916.4790 916.4654 14.9 0 36 0.059 +1Score > 43 indicates identity
Score > 37 indicates homology		 DAIVNAWK + Deamidated (NQ)
1019 482.7631 963.5116 963.5025 9.46 1 36 0.069 +1Score > 41 indicates identity
Score > 37 indicates homology		 ALFETVQR + Deamidated (NQ)
1539 606.3561 1210.6976 1210.7186 -17.3 1 36 0.066 +1Score > 36 indicates identity KWVGRPLIDK
315 329.7113 657.4080 657.4061 2.94 0 36 0.16 +1Score > 41 indicates identity
Score > 40 indicates homology		 IDVALK
304 324.1790 646.3434 646.3398 5.62 0 36 0.22 +1Score > 41 indicates identity SAISNR
1385 571.8074 1141.6002 1141.6091 -7.75 1 35 0.17 +1Score > 40 indicates identity LNELQQLGAR + Deamidated (NQ)
527 380.2356 758.4566 758.4650 -11.1 0 35 0.079 +1Score > 40 indicates identity
Score > 36 indicates homology		 LGLTVTR
793 438.7524 875.4902 875.4865 4.30 1 35 0.14 +1Score > 40 indicates identity
Score > 39 indicates homology		 LFAEQIR
744 429.2475 856.4804 856.4767 4.43 0 35 0.2 +1Score > 40 indicates identity LVAQGVNR + Deamidated (NQ)
363 682.3463 681.3390 681.3333 8.36 0 35 0.13 +1Score > 39 indicates identity
Score > 38 indicates homology		 NAYSVK + Deamidated (NQ)
485 372.2254 742.4362 742.4337 3.41 1 34 0.33 +1Score > 42 indicates identity INEVIR
1071 497.2989 992.5832 992.5767 6.61 0 34 0.071 +1Score > 35 indicates identity LHLNQLVR + Deamidated (NQ)
654 407.7534 813.4922 813.5072 -18.4 0 34 0.18 +1Score > 41 indicates identity
Score > 39 indicates homology		 NIILVSR
1002 479.7710 957.5274 957.5243 3.25 0 34 0.3 +1Score > 41 indicates identity IGAVGQAVSR + Deamidated (NQ)
1224 529.8045 1057.5944 1057.5880 6.13 1 34 0.2 +1Score > 41 indicates identity
Score > 40 indicates homology		 NNKANSLGLK
1065 497.2974 992.5802 992.5767 3.58 0 34 0.057 +1Score > 35 indicates identity
Score > 34 indicates homology		 LHLNQLVR + Deamidated (NQ)
1697 642.8278 1283.6410 1283.6357 4.17 1 34 0.24 +1Score > 40 indicates identity NIPADLENLQR + 2 Deamidated (NQ)
1459 586.2866 1170.5586 1170.5418 14.4 0 34 0.071 +1Score > 40 indicates identity
Score > 35 indicates homology		 DGGPAIYGHQR + Deamidated (NQ)
1696 642.8277 1283.6408 1283.6357 4.01 1 34 0.25 +1Score > 40 indicates identity NIPADLENLQR + 2 Deamidated (NQ)
1249 537.2981 1072.5816 1072.5764 4.86 1 34 0.33 -1Score > 41 indicates identity DDVLLLETR
814 444.2472 886.4798 886.4760 4.38 1 34 0.39 +1Score > 42 indicates identity LQEQLQK + Deamidated (NQ)
850 450.2861 898.5576 898.5488 9.90 0 34 0.11 +1Score > 37 indicates identity AVLDQLLK
1218 528.7960 1055.5774 1055.5723 4.85 1 33 0.25 +1Score > 40 indicates identity LQAQAQRLE
492 374.2126 746.4106 746.4109 -0.30 1 33 0.35 +1Score > 41 indicates identity SPKMLR + Oxidation (M)
1219 529.2875 1056.5604 1056.5815 -19.9 1 33 0.2 +1Score > 41 indicates identity
Score > 39 indicates homology		 LALQNEQLK + Deamidated (NQ)
359 676.4434 675.4361 675.4319 6.19 0 33 0.057 +1Score > 33 indicates identity GFLVLK
1553 608.8267 1215.6388 1215.6282 8.79 1 33 0.26 +1Score > 41 indicates identity
Score > 39 indicates homology		 VAMSIEGPTVGR
483 371.7462 741.4778 741.4861 -11.1 1 33 0.15 +1Score > 37 indicates identity LAKIAAR
1025 486.2829 970.5512 970.5447 6.74 1 33 0.07 +1Score > 41 indicates identity
Score > 34 indicates homology		 LLAQELQR + Deamidated (NQ)
678 415.7429 829.4712 829.4810 -11.8 1 33 0.44 +1Score > 42 indicates identity KIIDWR
433 715.4016 714.3943 714.3912 4.37 0 32 0.48 +1Score > 42 indicates identity
Score > 42 indicates homology		 DPVSVAK
1205 524.2667 1046.5188 1046.5396 -19.9 1 32 0.062 +1Score > 42 indicates identity
Score > 33 indicates homology		 NPDAKLFNK + Deamidated (NQ)
349 337.2173 672.4200 672.4282 -12.2 1 32 0.33 +1Score > 40 indicates identity SGLIRK
1338 561.8336 1121.6526 1121.6444 7.32 1 32 0.091 +1Score > 35 indicates identity
Score > 34 indicates homology		 IVPIIPDEAR
708 421.7600 841.5054 841.5022 3.92 0 32 0.061 +1Score > 40 indicates identity
Score > 33 indicates homology		 LGADVVLR
1672 635.3810 1268.7474 1268.7275 15.8 0 32 0.13 +1Score > 36 indicates identity LLVVLGNPAAMR + Oxidation (M)
177 544.3479 543.3406 543.3380 4.75 0 32 0.85 +1Score > 44 indicates identity IGAGVK
643 808.4230 807.4157 807.4126 3.83 1 32 0.54 +1Score > 42 indicates identity ENYLAAK
496 747.4801 746.4728 746.4691 5.05 0 32 0.065 +1Score > 32 indicates identity FQIVLK
1035 487.2716 972.5286 972.5240 4.76 0 32 0.13 +1Score > 42 indicates identity
Score > 36 indicates homology		 GGLVTQVNGK + Deamidated (NQ)
1041 488.7529 975.4912 975.5059 -15.0 1 32 0.15 +1Score > 42 indicates identity
Score > 36 indicates homology		 KASVAMLGAE
844 300.5076 898.5010 898.4985 2.80 1 32 0.2 +1Score > 39 indicates identity
Score > 37 indicates homology		 VPGRSIDR
431 357.7302 713.4458 713.4436 3.21 0 31 0.41 +1Score > 40 indicates identity INGVALK
942 467.7746 933.5346 933.5284 6.73 0 31 0.12 +1Score > 38 indicates identity
Score > 35 indicates homology		 FIDGLTIR
1173 515.3078 1028.6010 1028.5866 14.1 1 31 0.19 +1Score > 38 indicates identity
Score > 37 indicates homology		 LLIEADLSR
320 330.1972 658.3798 658.3762 5.51 0 31 0.17 +1Score > 42 indicates identity
Score > 36 indicates homology		 IQVSGR
339 671.3760 670.3687 670.3650 5.59 0 31 0.19 +1Score > 36 indicates identity DGALAPK
1062 993.5367 992.5294 992.5291 0.34 0 31 0.35 +1Score > 41 indicates identity
Score > 39 indicates homology		 SYLDIGVAR
729 425.7255 849.4364 849.4344 2.35 0 31 0.098 +1Score > 42 indicates identity
Score > 33 indicates homology		 YNDAVIR
357 675.4066 674.3993 674.3963 4.50 0 31 0.4 +1Score > 39 indicates identity QVTISK
830 447.7393 893.4640 893.4606 3.81 1 31 0.11 +1Score > 41 indicates identity
Score > 34 indicates homology		 SLLNEYR
308 326.1651 650.3156 650.3136 3.13 0 31 0.18 +1Score > 43 indicates identity
Score > 36 indicates homology		 NAFSGR
262 629.3658 628.3585 628.3544 6.54 0 31 0.13 +1Score > 37 indicates identity
Score > 34 indicates homology		 AVNPTK
829 894.4711 893.4638 893.4607 3.54 1 30 0.71 +1Score > 41 indicates identity GISEGLYR
512 753.4281 752.4208 752.4181 3.65 0 30 0.15 +1Score > 38 indicates identity
Score > 35 indicates homology		 ALDVHAK
796 879.4969 878.4896 878.4895 0.12 1 30 0.37 +1Score > 39 indicates identity AKMSIVSK + Oxidation (M)
1654 631.3541 1260.6936 1260.6747 15.0 1 30 0.36 +1Score > 39 indicates identity
Score > 38 indicates homology		 MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
251 311.1719 620.3292 620.3282 1.69 0 30 0.25 +1Score > 42 indicates identity
Score > 37 indicates homology		 FADIR
353 337.6968 673.3790 673.3871 -12.0 1 30 0.079 +1Score > 42 indicates identity
Score > 32 indicates homology		 GGKSAVR
490 373.2145 744.4144 744.4130 1.95 1 30 0.35 +1Score > 44 indicates identity
Score > 38 indicates homology		 ITEGGLR
732 851.4177 850.4104 850.4072 3.76 1 30 0.22 +1Score > 42 indicates identity
Score > 36 indicates homology		 QIEQFGK + 2 Deamidated (NQ)
1653 631.3531 1260.6916 1260.6965 -3.87 0 30 0.47 +1Score > 39 indicates identity QFILSQVALLE + Deamidated (NQ)
1069 497.2982 992.5818 992.5767 5.20 0 30 0.073 +1Score > 35 indicates identity
Score > 31 indicates homology		 LHLNQLVR + Deamidated (NQ)
677 415.7427 829.4708 829.4658 6.12 0 30 0.22 +1Score > 43 indicates identity
Score > 36 indicates homology		 VALVTDGR
321 330.6893 659.3640 659.3602 5.81 1 30 0.1 +1Score > 42 indicates identity
Score > 33 indicates homology		 AETAIR
595 394.7179 787.4212 787.4076 17.4 0 30 0.57 +1Score > 43 indicates identity
Score > 40 indicates homology		 LDLNDAK
1512 400.5573 1198.6501 1198.6557 -4.71 1 30 0.17 +1Score > 40 indicates identity
Score > 35 indicates homology		 QLQEILDIAR + Deamidated (NQ)
1111 505.2639 1008.5132 1008.5287 -15.3 1 30 0.077 +1Score > 41 indicates identity
Score > 31 indicates homology		 HKAPGAAAMR
1072 993.5941 992.5868 992.5767 10.2 0 30 0.12 +1Score > 33 indicates identity HLIDNILR
307 651.3218 650.3145 650.3136 1.38 0 30 0.91 +1Score > 43 indicates identity
Score > 42 indicates homology		 QGFSGR
1178 516.2645 1030.5144 1030.5182 -3.66 1 30 0.47 +1Score > 42 indicates identity
Score > 39 indicates homology		 LTNKSIQPE + 2 Deamidated (NQ)
324 662.3505 661.3432 661.3395 5.64 0 30 0.79 +1Score > 41 indicates identity NTATVR + Deamidated (NQ)
556 386.7212 771.4278 771.4239 5.13 0 30 0.27 +1Score > 42 indicates identity
Score > 36 indicates homology		 IGGADLAR
1216 527.8388 1053.6630 1053.6546 8.02 1 30 0.12 +1Score > 33 indicates identity VAIAALEVIR
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