MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 91–100 (out of 250)

Page: Previous 1 …5 6 7 8 9 10 11 12 13 14 15 … 25 Next 

+91

Accession Score Description
1 GPMI_PSEPG 71 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas putida (strain GB-1) GN=gpmI PE=3 SV=1

+92

Accession Score Description
1 SYGB_PSEPG 70 Glycyl-tRNA synthetase beta subunit OS=Pseudomonas putida (strain GB-1) GN=glyS PE=3 SV=1

+93

Accession Score Description
1 ASPQ_PSEPK 69 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+94

Accession Score Description
1 RL21_PSEE4 69 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+95

Accession Score Description
1 RL29_BACAA 69 50S ribosomal protein L29 OS=Bacillus anthracis (strain A0248) GN=rpmC PE=3 SV=1

+96

Accession Score Description
1 ATPF_PSEPK 66 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

-97

Accession Score Description
1 SYI_PSEP1 62 Isoleucyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ileS PE=3 SV=1
Score Mass Matches Sequences emPAI
97.1 SYI_PSEP1 62 106663 3 (2) 3 (2) 0.04
Isoleucyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ileS PE=3 SV=1
3 samesets of SYI_PSEP1
SYI_PSEPG 62 106721 3 (2) 3 (2) 0.04
Isoleucyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=ileS PE=3 SV=1
SYI_PSEPK 62 106590 3 (2) 3 (2) 0.04
Isoleucyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=ileS PE=3 SV=1
SYI_PSEPW 62 106606 3 (2) 3 (2) 0.04
Isoleucyl-tRNA synthetase OS=Pseudomonas putida (strain W619) GN=ileS PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
178   547.3304 546.3231 546.3199 5.82 0 8 6 +6Score > 41 indicates identity
Score > 28 indicates homology		 R.VMAVK.A
2011   718.8885 1435.7624 1435.7460 11.5 0 65 0.00012 +1Score > 39 indicates identity
Score > 38 indicates homology		 U R.FLLSNLSGFDPAR.D
2043   485.6073 1453.8001 1453.7870 8.96 0 25 0.0094 +1Score > 38 indicates identity
Score > 18 indicates homology		 U R.NWGVPIPFFLHK.Q

2 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
SYI_PSEE4 65 106711 97.1
Isoleucyl-tRNA synthetase OS=Pseudomonas entomophila (strain L48) GN=ileS PE=3 SV=1
SYI_AZOSB 25 104865 97.1
Isoleucyl-tRNA synthetase OS=Azoarcus sp. (strain BH72) GN=ileS PE=3 SV=1
10 samesets of SYI_AZOSB
SYI_PSEA7 25 106022
Isoleucyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain PA7) GN=ileS PE=3 SV=1
SYI_DECAR 25 105013
Isoleucyl-tRNA synthetase OS=Dechloromonas aromatica (strain RCB) GN=ileS PE=3 SV=1
SYI_AZOVD 25 106467
Isoleucyl-tRNA synthetase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=ileS PE=3 SV=1
SYI_PSEMY 25 106129
Isoleucyl-tRNA synthetase OS=Pseudomonas mendocina (strain ymp) GN=ileS PE=3 SV=1
SYI_PSEAB 25 106187
Isoleucyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=ileS PE=3 SV=1
SYI_PSEAE 25 106254
Isoleucyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=ileS PE=3 SV=1
SYI_PSESM 25 106693
Isoleucyl-tRNA synthetase OS=Pseudomonas syringae pv. tomato GN=ileS PE=3 SV=1
SYI_PSEU2 25 106589
Isoleucyl-tRNA synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=ileS PE=3 SV=1
SYI_PSEA8 25 106241
Isoleucyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain LESB58) GN=ileS PE=3 SV=1
SYI_PSE14 25 106645
Isoleucyl-tRNA synthetase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=ileS PE=3 SV=1

+98

Accession Score Description
1 EFP_PSEPG 65 Elongation factor P OS=Pseudomonas putida (strain GB-1) GN=efp PE=3 SV=1

+99

Accession Score Description
1 DADA_PSEPG 64 D-amino acid dehydrogenase small subunit OS=Pseudomonas putida (strain GB-1) GN=dadA PE=3 SV=1

+100

Accession Score Description
1 RL2_PSEE4 64 50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1
Page: Previous 1 …5 6 7 8 9 10 11 12 13 14 15 … 25 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/