MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 250)


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+91

Accession Score Description
1 GPMI_PSEPG 71 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas putida (strain GB-1) GN=gpmI PE=3 SV=1

+92

Accession Score Description
1 SYGB_PSEPG 70 Glycyl-tRNA synthetase beta subunit OS=Pseudomonas putida (strain GB-1) GN=glyS PE=3 SV=1

+93

Accession Score Description
1 ASPQ_PSEPK 69 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+94

Accession Score Description
1 RL21_PSEE4 69 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

-95

Accession Score Description
1 RL29_BACAA 69 50S ribosomal protein L29 OS=Bacillus anthracis (strain A0248) GN=rpmC PE=3 SV=1
Score Mass Matches Sequences emPAI
95.1 RL29_BACAA 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus anthracis (strain A0248) GN=rpmC PE=3 SV=1
10 samesets of RL29_BACAA
RL29_BACAC 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) GN=rpmC PE=3 SV=1
RL29_BACAN 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus anthracis GN=rpmC PE=3 SV=1
RL29_BACC0 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus cereus (strain AH820) GN=rpmC PE=3 SV=1
RL29_BACC1 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus cereus (strain ATCC 10987) GN=rpmC PE=3 SV=2
RL29_BACC2 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus cereus (strain G9842) GN=rpmC PE=3 SV=1
RL29_BACC3 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus cereus (strain 03BB102) GN=rpmC PE=3 SV=1
RL29_BACC4 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus cereus (strain B4264) GN=rpmC PE=3 SV=1
RL29_BACC7 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus cereus (strain AH187) GN=rpmC PE=3 SV=1
RL29_BACCN 69 7763 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus cereus subsp. cytotoxis (strain NVH 391-98) GN=rpmC PE=3 SV=1
RL29_BACWK 69 7767 1 (1) 1 (1) 0.32
50S ribosomal protein L29 OS=Bacillus weihenstephanensis (strain KBAB4) GN=rpmC PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2086   737.8900 1473.7654 1473.7576 5.34 1 69 8.2e-005 +1Score > 41 indicates identity U R.FQLATGQLENPTR.I

+96

Accession Score Description
1 ATPF_PSEPK 66 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+97

Accession Score Description
1 SYI_PSEP1 62 Isoleucyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ileS PE=3 SV=1

+98

Accession Score Description
1 EFP_PSEPG 65 Elongation factor P OS=Pseudomonas putida (strain GB-1) GN=efp PE=3 SV=1

+99

Accession Score Description
1 DADA_PSEPG 64 D-amino acid dehydrogenase small subunit OS=Pseudomonas putida (strain GB-1) GN=dadA PE=3 SV=1

+100

Accession Score Description
1 RL2_PSEE4 64 50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1
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