MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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91| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GPMI_PSEPG | 71 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pseudomonas putida (strain GB-1) GN=gpmI PE=3 SV=1 |
92| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYGB_PSEPG | 70 | Glycyl-tRNA synthetase beta subunit OS=Pseudomonas putida (strain GB-1) GN=glyS PE=3 SV=1 |
93| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ASPQ_PSEPK | 69 | Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 93.1 | ASPQ_PSEPK |
69 | 38584 | 2 (2) | 2 (2) | 0.12 | |
| Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1167 |
512.8148 | 1023.6150 | 1023.6077 | 7.21 | 1 | 35 | 0.0084 | 1Score > 34 indicates identity Score > 27 indicates homology |
U | R.VVPALQELR.K | |
| 2257 |
775.9525 | 1549.8904 | 1549.8828 | 4.96 | 1 | 65 | 4.8e-005 | 1Score > 34 indicates identity |
U | K.LIAGVPELADIANVR.G |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ASPQ_PSES7 |
65 | 36178 | 93.1 | ||
| Glutaminase-asparaginase OS=Pseudomonas sp. (strain 7A) GN=ansB PE=1 SV=2 | |||||
| ASPQ_PSEFA |
35 | 38711 | 93.1 | ||
| Glutaminase-asparaginase OS=Pseudomonas fluorescens biotype A GN=ansB PE=3 SV=1 | |||||
94| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEE4 | 69 | 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1 |
95| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL29_BACAA | 69 | 50S ribosomal protein L29 OS=Bacillus anthracis (strain A0248) GN=rpmC PE=3 SV=1 |
96| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPK | 66 | ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1 |
97| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYI_PSEP1 | 62 | Isoleucyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ileS PE=3 SV=1 |
98| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFP_PSEPG | 65 | Elongation factor P OS=Pseudomonas putida (strain GB-1) GN=efp PE=3 SV=1 |
99| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DADA_PSEPG | 64 | D-amino acid dehydrogenase small subunit OS=Pseudomonas putida (strain GB-1) GN=dadA PE=3 SV=1 |
100| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEE4 | 64 | 50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1 |
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