MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 250)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 25 Next 

+81

Accession Score Description
1 ODO2_AZOVI 77 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Azotobacter vinelandii GN=sucB PE=1 SV=2

+82

Accession Score Description
1 DHSB_ECOLI 76 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+83

Accession Score Description
1 GCSP1_PSEPK 75 Glycine dehydrogenase [decarboxylating] 1 OS=Pseudomonas putida (strain KT2440) GN=gcvP1 PE=3 SV=1

+84

Accession Score Description
1 CYSNC_PSEAE 75 Bifunctional enzyme cysN/cysC OS=Pseudomonas aeruginosa GN=cysNC PE=3 SV=1

+85

Accession Score Description
1 RL14_DECAR 74 50S ribosomal protein L14 OS=Dechloromonas aromatica (strain RCB) GN=rplN PE=3 SV=1

+86

Accession Score Description
1 NDK_PSEPG 73 Nucleoside diphosphate kinase OS=Pseudomonas putida (strain GB-1) GN=ndk PE=3 SV=1

+87

Accession Score Description
1 ATPG_PSEFS 73 ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1

+88

Accession Score Description
1 ETFA_PSEAE 73 Electron transfer flavoprotein subunit alpha OS=Pseudomonas aeruginosa GN=etfA PE=3 SV=1

+89

Accession Score Description
1 ARLY_PSE14 73 Argininosuccinate lyase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=argH PE=3 SV=1

-90

Accession Score Description
1 CARB_PSEPK 72 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2
Score Mass Matches Sequences emPAI
90.1 CARB_PSEPK 72 118633 5 (3) 5 (3) 0.06
Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
247   309.1787 616.3428 616.3432 -0.52 1 22 0.52 +1Score > 47 indicates identity
Score > 32 indicates homology		 E.LDELK.R
366   341.6990 681.3834 681.3810 3.64 1 20 0.61 +1Score > 37 indicates identity
Score > 30 indicates homology		 R.HGVLEK.F
1760   657.3738 1312.7330 1312.7238 7.04 1 42 0.0014 +1Score > 39 indicates identity
Score > 26 indicates homology		 U K.VDLASPEAASILK.R
2485   827.4263 1652.8380 1652.8298 5.02 0 57 3.4e-005 +1Score > 40 indicates identity
Score > 25 indicates homology		 U R.TPASFEPSIDYVVTK.L
3004   650.3463 1948.0171 1947.9902 13.8 1 17 0.039 +1Score > 39 indicates identity
Score > 16 indicates homology		 U K.LAIGYTLDELQNDITGGR.T

1 subset or intersection (13 subset proteins in total)

Score Mass Subset of
CARB_ECO57 57 118580 90.1
Carbamoyl-phosphate synthase large chain OS=Escherichia coli O157:H7 GN=carB PE=3 SV=2
12 samesets of CARB_ECO57
CARB_HALER 57 118169
Carbamoyl-phosphate synthase large chain OS=Halomonas eurihalina GN=carB PE=3 SV=1
CARB_ECOLI 57 118566
Carbamoyl-phosphate synthase large chain OS=Escherichia coli (strain K12) GN=carB PE=1 SV=2
CARB_SALTI 57 118865
Carbamoyl-phosphate synthase large chain OS=Salmonella typhi GN=carB PE=3 SV=3
CARB_SALTY 57 118863
Carbamoyl-phosphate synthase large chain OS=Salmonella typhimurium GN=carB PE=3 SV=4
CARB_SHIFL 57 118580
Carbamoyl-phosphate synthase large chain OS=Shigella flexneri GN=carB PE=3 SV=2
CARB_ECOL6 57 118615
Carbamoyl-phosphate synthase large chain OS=Escherichia coli O6 GN=carB PE=3 SV=3
CARB_NEIGO 57 118422
Carbamoyl-phosphate synthase large chain OS=Neisseria gonorrhoeae GN=carB PE=3 SV=2
CARB_NEIMA 57 118429
Carbamoyl-phosphate synthase large chain OS=Neisseria meningitidis serogroup A GN=carB PE=3 SV=1
CARB_NEIMB 57 118385
Carbamoyl-phosphate synthase large chain OS=Neisseria meningitidis serogroup B GN=carB PE=3 SV=1
CARB_PSESM 57 118714
Carbamoyl-phosphate synthase large chain OS=Pseudomonas syringae pv. tomato GN=carB PE=3 SV=1
CARB_XYLFT 57 118251
Carbamoyl-phosphate synthase large chain OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=carB PE=3 SV=1
CARB_YERPE 57 119025
Carbamoyl-phosphate synthase large chain OS=Yersinia pestis GN=carB PE=3 SV=3
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