MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1177 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 5 | EFTU_CARRP | 73 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1045 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
| 3 | EFTU_BACAA | 271 | Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1 | |
| 4 | EFTU_ANATD | 83 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 525 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 5 | ATPG_RHOP5 | 40 | description | |
| 3 | ATPB_LEGPA | 167 | ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1 | |
| 2 | CH60_PSEPK | 473 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 6 | CH602_PROMS | 39 | 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1 | |
| 4 | CH602_SINMW | 59 | 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPG | 522 | ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 112 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 463 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 391 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPG | 350 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1 | |
| 2 | RPOC_PSEA7 | 244 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1 | |
| 3 | FTSZ_PSEPK | 95 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 349 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 327 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 321 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 9.1 | ACON2_PSEAE |
321 | 94196 | 22 (13) | 12 (8) | 0.24 | |
| Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 | |||||||
22 peptide matches (15 non-duplicate, 7 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 258 |
626.3894 | 625.3821 | 625.3799 | 3.54 | 0 | 24 | 0.13 | 1Score > 32 indicates identity Score > 28 indicates homology |
U | K.IPVVQA.- | |
| 540 |
382.7202 | 763.4258 | 763.4228 | 3.95 | 0 | 10 | 0.28 | 1Score > 41 indicates identity Score > 17 indicates homology |
U | K.GFTLAQK.M | |
| 541 |
764.4333 | 763.4260 | 763.4228 | 4.18 | 0 | 42 | 0.049 | 1Score > 41 indicates identity |
U | K.GFTLAQK.M | |
| 549 |
2 |
384.2626 | 766.5106 | 766.5065 | 5.41 | 0 | 40 | 0.0027 | 1Score > 27 indicates identity |
U | R.IPLIVGR.G |
| 837 |
449.2816 | 896.5486 | 896.5443 | 4.80 | 0 | 25 | 0.17 | 1Score > 34 indicates identity Score > 29 indicates homology |
U | K.LQPGITLR.D | |
| 915 |
1 |
463.2811 | 924.5476 | 924.5433 | 4.73 | 0 | 25 | 0.6 | 2Score > 36 indicates identity |
U | R.LWLAPPTK.M |
| 1623 |
1 |
622.8323 | 1243.6500 | 1243.6449 | 4.18 | 1 | 55 | 6.5e-005 | 1Score > 41 indicates identity Score > 26 indicates homology |
U | R.VPPGVDEAAYVK.A |
| 1641 |
628.2876 | 1254.5606 | 1254.5550 | 4.47 | 0 | 49 | 0.00016 | 1Score > 40 indicates identity Score > 23 indicates homology |
U | K.DIDSMAADVYR.Y | |
| 1785 |
661.8517 | 1321.6888 | 1321.6838 | 3.83 | 0 | 85 | 2.2e-006 | 1Score > 40 indicates identity |
U | R.VQTGSTVVSTSTR.N | |
| 1909 |
1 |
694.8531 | 1387.6916 | 1387.6772 | 10.4 | 1 | 64 | 0.0002 | 1Score > 41 indicates identity Score > 40 indicates homology |
U | R.YLSFDQIAEFR.E |
| 2510 |
834.4295 | 1666.8444 | 1666.8315 | 7.75 | 0 | 72 | 2.7e-006 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | K.GFPVAYVGDVVGTGSSR.K | |
| 2511 |
556.6239 | 1666.8499 | 1666.8315 | 11.0 | 0 | 52 | 1.9e-005 | 1Score > 40 indicates identity Score > 17 indicates homology |
U | K.GFPVAYVGDVVGTGSSR.K | |
| 2982 |
645.3542 | 1933.0408 | 1933.0282 | 6.49 | 0 | 25 | 0.068 | 1Score > 37 indicates identity Score > 26 indicates homology |
U | R.GGVSLRPGDGIIHSWLNR.M | |
| 2983 |
2 |
484.2675 | 1933.0409 | 1933.0282 | 6.55 | 0 | 19 | 0.18 | 1Score > 37 indicates identity Score > 24 indicates homology |
U | R.GGVSLRPGDGIIHSWLNR.M |
| 4570 |
832.9310 | 3327.6949 | 3327.6445 | 15.1 | 1 | 17 | 0.028 | 1Score > 35 indicates identity Score > 14 indicates homology |
U | K.VPGETNTDDLSPAPDAWSRPDIPLHALAMLK.M + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ACON2_SYNY3 |
89 | 94290 | 9.1 | ||
| Aconitate hydratase 2 OS=Synechocystis sp. (strain PCC 6803) GN=acnB PE=3 SV=1 | |||||
| ACON2_ECOLI |
84 | 94009 | 9.1 | ||
| Aconitate hydratase 2 OS=Escherichia coli (strain K12) GN=acnB PE=1 SV=3 | |||||
| RIBA_BAUCH |
55 | 22461 | 9.1 | ||
| GTP cyclohydrolase-2 OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=ribA PE=3 SV=1 | |||||
| BXL7_ARATH |
34 | 84751 | 9.1 | ||
| Probable beta-D-xylosidase 7 OS=Arabidopsis thaliana GN=BXL7 PE=2 SV=2 | |||||
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 313 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
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