MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 250)

Page: Previous 1 …4 5 6 7 8 9 10 11 12 13 14 … 25 Next 

+81

Accession Score Description
1 ODO2_AZOVI 77 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Azotobacter vinelandii GN=sucB PE=1 SV=2

+82

Accession Score Description
1 DHSB_ECOLI 76 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+83

Accession Score Description
1 GCSP1_PSEPK 75 Glycine dehydrogenase [decarboxylating] 1 OS=Pseudomonas putida (strain KT2440) GN=gcvP1 PE=3 SV=1

+84

Accession Score Description
1 CYSNC_PSEAE 75 Bifunctional enzyme cysN/cysC OS=Pseudomonas aeruginosa GN=cysNC PE=3 SV=1

+85

Accession Score Description
1 RL14_DECAR 74 50S ribosomal protein L14 OS=Dechloromonas aromatica (strain RCB) GN=rplN PE=3 SV=1

+86

Accession Score Description
1 NDK_PSEPG 73 Nucleoside diphosphate kinase OS=Pseudomonas putida (strain GB-1) GN=ndk PE=3 SV=1

+87

Accession Score Description
1 ATPG_PSEFS 73 ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1

+88

Accession Score Description
1 ETFA_PSEAE 73 Electron transfer flavoprotein subunit alpha OS=Pseudomonas aeruginosa GN=etfA PE=3 SV=1

-89

Accession Score Description
1 ARLY_PSE14 73 Argininosuccinate lyase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=argH PE=3 SV=1
Score Mass Matches Sequences emPAI
89.1 ARLY_PSE14 73 52053 4 (1) 4 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=argH PE=3 SV=1
19 samesets of ARLY_PSE14
ARLY_PSEU2 73 52072 3 (1) 3 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=argH PE=3 SV=1
ARLY_PSEFS 73 51773 3 (1) 3 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas fluorescens (strain SBW25) GN=argH PE=3 SV=1
ARLY_PSEPG 73 51862 3 (1) 3 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas putida (strain GB-1) GN=argH PE=3 SV=1
ARLY_PSEPK 73 51879 3 (1) 3 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas putida (strain KT2440) GN=argH PE=3 SV=2
ARLY2_PSEPF 73 51877 3 (1) 3 (1) 0.04
Argininosuccinate lyase 2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=argH2 PE=3 SV=1
ARLY_AZOVD 73 51925 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=argH PE=3 SV=1
ARLY_PSEAB 73 51893 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=argH PE=3 SV=1
ARLY_PSEAE 73 51893 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas aeruginosa GN=argH PE=3 SV=2
ARLY_PSEMY 73 51633 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas mendocina (strain ymp) GN=argH PE=3 SV=1
ARLY_PSEPW 73 51835 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas putida (strain W619) GN=argH PE=3 SV=1
ARLY_PSESM 73 52055 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas syringae pv. tomato GN=argH PE=3 SV=1
ARLY_PSEE4 73 51918 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas entomophila (strain L48) GN=argH PE=3 SV=1
ARLY_PSEF5 73 51858 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=argH PE=3 SV=1
ARLY_PSEU5 73 51681 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas stutzeri (strain A1501) GN=argH PE=3 SV=1
ARLY_PSEA7 73 51871 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas aeruginosa (strain PA7) GN=argH PE=3 SV=1
ARLY_PSEA8 73 51918 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Pseudomonas aeruginosa (strain LESB58) GN=argH PE=3 SV=1
ARLY_HAHCH 73 52463 2 (1) 2 (1) 0.04
Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) GN=argH PE=3 SV=1
ARLY_CHRSD 73 52020 1 (1) 1 (1) 0.04
Argininosuccinate lyase OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=argH PE=3 SV=1
ARLY_SACD2 73 52383 1 (1) 1 (1) 0.04
Argininosuccinate lyase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=argH PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
163   515.3350 514.3277 514.3227 9.72 0 8 6.5 +10Score > 44 indicates identity
Score > 29 indicates homology		 R.AAVVR.G
1545   404.8954 1211.6644 1211.6622 1.78 1 12 0.94 +1Score > 39 indicates identity
Score > 24 indicates homology		 U R.LQQGLLGQAER.E
1607   619.3192 1236.6238 1236.6139 8.05 0 27 0.07 +1Score > 41 indicates identity
Score > 28 indicates homology		 U R.FSEPVDAFVAR.F
2205   764.8953 1527.7760 1527.7569 12.5 0 73 3.2e-005 +1Score > 40 indicates identity U R.GFSTATDLADYLVR.R

+90

Accession Score Description
1 CARB_PSEPK 72 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2
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