MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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81| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_AZOVI | 77 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Azotobacter vinelandii GN=sucB PE=1 SV=2 |
82| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSB_ECOLI | 76 | Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1 |
83| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GCSP1_PSEPK | 75 | Glycine dehydrogenase [decarboxylating] 1 OS=Pseudomonas putida (strain KT2440) GN=gcvP1 PE=3 SV=1 |
84| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CYSNC_PSEAE | 75 | Bifunctional enzyme cysN/cysC OS=Pseudomonas aeruginosa GN=cysNC PE=3 SV=1 |
85| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL14_DECAR | 74 | 50S ribosomal protein L14 OS=Dechloromonas aromatica (strain RCB) GN=rplN PE=3 SV=1 |
86| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NDK_PSEPG | 73 | Nucleoside diphosphate kinase OS=Pseudomonas putida (strain GB-1) GN=ndk PE=3 SV=1 |
87| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPG_PSEFS | 73 | ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1 |
88| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ETFA_PSEAE | 73 | Electron transfer flavoprotein subunit alpha OS=Pseudomonas aeruginosa GN=etfA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 88.1 | ETFA_PSEAE |
73 | 31404 | 3 (2) | 2 (2) | 0.15 | |
| Electron transfer flavoprotein subunit alpha OS=Pseudomonas aeruginosa GN=etfA PE=3 SV=1 | |||||||
3 peptide matches (3 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1269 |
363.1980 | 1086.5722 | 1086.5669 | 4.84 | 1 | 22 | 5.1 | 3Score > 42 indicates identity |
U | K.SDRPELTAAK.I | |
| 1270 |
544.2939 | 1086.5732 | 1086.5669 | 5.83 | 1 | 56 | 0.0024 | 1Score > 42 indicates identity |
U | K.SDRPELTAAK.I | |
| 2932 |
952.9651 | 1903.9156 | 1903.9098 | 3.05 | 0 | 52 | 0.00019 | 1Score > 40 indicates identity Score > 27 indicates homology |
U | R.AAVDAGFVPNDMQVGQTGK.I |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ETFA_HUMAN |
52 | 35400 | 88.1 | ||
| Electron transfer flavoprotein subunit alpha, mitochondrial OS=Homo sapiens GN=ETFA PE=1 SV=1 | |||||
| 4 samesets of ETFA_HUMAN | |||||
| ETFA_MACFA |
52 | 35362 | |||
| Electron transfer flavoprotein subunit alpha, mitochondrial OS=Macaca fascicularis GN=ETFA PE=2 SV=1 | |||||
| ETFA_MOUSE |
52 | 35330 | |||
| Electron transfer flavoprotein subunit alpha, mitochondrial OS=Mus musculus GN=Etfa PE=1 SV=2 | |||||
| ETFA_PONAB |
52 | 35486 | |||
| Electron transfer flavoprotein subunit alpha, mitochondrial OS=Pongo abelii GN=ETFA PE=2 SV=1 | |||||
| ETFA_RAT |
52 | 35272 | |||
| Electron transfer flavoprotein subunit alpha, mitochondrial OS=Rattus norvegicus GN=Etfa PE=1 SV=4 | |||||
89| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARLY_PSE14 | 73 | Argininosuccinate lyase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=argH PE=3 SV=1 |
90| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CARB_PSEPK | 72 | Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2 |
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