MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 250)


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+81

Accession Score Description
1 ODO2_AZOVI 77 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Azotobacter vinelandii GN=sucB PE=1 SV=2

+82

Accession Score Description
1 DHSB_ECOLI 76 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+83

Accession Score Description
1 GCSP1_PSEPK 75 Glycine dehydrogenase [decarboxylating] 1 OS=Pseudomonas putida (strain KT2440) GN=gcvP1 PE=3 SV=1

+84

Accession Score Description
1 CYSNC_PSEAE 75 Bifunctional enzyme cysN/cysC OS=Pseudomonas aeruginosa GN=cysNC PE=3 SV=1

+85

Accession Score Description
1 RL14_DECAR 74 50S ribosomal protein L14 OS=Dechloromonas aromatica (strain RCB) GN=rplN PE=3 SV=1

+86

Accession Score Description
1 NDK_PSEPG 73 Nucleoside diphosphate kinase OS=Pseudomonas putida (strain GB-1) GN=ndk PE=3 SV=1

-87

Accession Score Description
1 ATPG_PSEFS 73 ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1
Score Mass Matches Sequences emPAI
87.1 ATPG_PSEFS 73 31488 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1
13 samesets of ATPG_PSEFS
ATPG_PSEPF 73 31518 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf0-1) GN=atpG PE=3 SV=1
ATPG_PSEPG 73 31653 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1
ATPG_PSEPK 73 31558 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
ATPG_PSEPW 73 31545 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas putida (strain W619) GN=atpG PE=3 SV=1
ATPG_PSEP1 73 31558 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpG PE=3 SV=1
ATPG_PSEU2 73 31458 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpG PE=3 SV=1
ATPG_PSEE4 73 31602 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas entomophila (strain L48) GN=atpG PE=3 SV=1
ATPG_PSEF5 73 31532 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpG PE=3 SV=1
ATPG_PSE14 73 31458 2 (1) 2 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpG PE=3 SV=1
ATPG_PSEAB 73 31704 1 (1) 1 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpG PE=3 SV=1
ATPG_PSEAE 73 31704 1 (1) 1 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas aeruginosa GN=atpG PE=3 SV=1
ATPG_PSEA7 73 31704 1 (1) 1 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain PA7) GN=atpG PE=3 SV=1
ATPG_PSEA8 73 31704 1 (1) 1 (1) 0.07
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain LESB58) GN=atpG PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
491   373.7269 745.4392 745.4334 7.85 0 22 1.3 +2Score > 39 indicates identity
Score > 36 indicates homology
R.LSVVSNK.F
2343   528.2791 1581.8155 1581.7899 16.2 1 73 5e-007 +1Score > 40 indicates identity
Score > 23 indicates homology
U R.QVIGHLANANPEYR.H + Deamidated (NQ)

+88

Accession Score Description
1 ETFA_PSEAE 73 Electron transfer flavoprotein subunit alpha OS=Pseudomonas aeruginosa GN=etfA PE=3 SV=1

+89

Accession Score Description
1 ARLY_PSE14 73 Argininosuccinate lyase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=argH PE=3 SV=1

+90

Accession Score Description
1 CARB_PSEPK 72 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2
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