MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 250)


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-81

Accession Score Description
1 ODO2_AZOVI 77 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Azotobacter vinelandii GN=sucB PE=1 SV=2
Score Mass Matches Sequences emPAI
81.1 ODO2_AZOVI 77 41976 4 (4) 2 (2) 0.17
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Azotobacter vinelandii GN=sucB PE=1 SV=2
1 sameset of ODO2_AZOVI
ODO2_PSEAE 77 42861 4 (4) 2 (2) 0.17
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+4 peptide matches (3 non-duplicate, 1 duplicate)

2 subsets and intersections (13 subset proteins in total)

Score Mass Subset of
ODO2_BUCAI 60 48175 81.1
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=sucB PE=3 SV=1
5 samesets of ODO2_BUCAI
ODO2_RALEH 60 43223
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=sucB PE=3 SV=1
ODO2_RICCN 60 42829
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia conorii GN=sucB PE=3 SV=1
ODO2_RICFE 60 43491
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia felis GN=sucB PE=3 SV=1
ODO2_RICPR 60 44123
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia prowazekii GN=sucB PE=3 SV=1
ODO2_RICTY 60 43853
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Rickettsia typhi GN=sucB PE=3 SV=1
ODO2_BOVIN 44 49284 81.1
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Bos taurus GN=DLST PE=1 SV=2
6 samesets of ODO2_BOVIN
ODO2_MOUSE 44 49306
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Mus musculus GN=Dlst PE=1 SV=1
ODO2_RAT 44 49236
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Rattus norvegicus GN=Dlst PE=1 SV=2
ODO2_DICDI 44 47775
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Dictyostelium discoideum GN=odhB PE=1 SV=1
ODO2_HUMAN 44 49041
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Homo sapiens GN=DLST PE=1 SV=3
ODO2_PIG 44 49288
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS=Sus scrofa GN=DLST PE=1 SV=1
ODO2_TAKRU 44 44255
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (Fragment) OS=Takifugu rubripes GN=dlst PE=3 SV=1

+82

Accession Score Description
1 DHSB_ECOLI 76 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+83

Accession Score Description
1 GCSP1_PSEPK 75 Glycine dehydrogenase [decarboxylating] 1 OS=Pseudomonas putida (strain KT2440) GN=gcvP1 PE=3 SV=1

+84

Accession Score Description
1 CYSNC_PSEAE 75 Bifunctional enzyme cysN/cysC OS=Pseudomonas aeruginosa GN=cysNC PE=3 SV=1

+85

Accession Score Description
1 RL14_DECAR 74 50S ribosomal protein L14 OS=Dechloromonas aromatica (strain RCB) GN=rplN PE=3 SV=1

+86

Accession Score Description
1 NDK_PSEPG 73 Nucleoside diphosphate kinase OS=Pseudomonas putida (strain GB-1) GN=ndk PE=3 SV=1

+87

Accession Score Description
1 ATPG_PSEFS 73 ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1

+88

Accession Score Description
1 ETFA_PSEAE 73 Electron transfer flavoprotein subunit alpha OS=Pseudomonas aeruginosa GN=etfA PE=3 SV=1

+89

Accession Score Description
1 ARLY_PSE14 73 Argininosuccinate lyase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=argH PE=3 SV=1

+90

Accession Score Description
1 CARB_PSEPK 72 Carbamoyl-phosphate synthase large chain OS=Pseudomonas putida (strain KT2440) GN=carB PE=3 SV=2
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