MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 250)


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+71

Accession Score Description
1 CH10_PSEP1 87 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1

+72

Accession Score Description
1 Y4497_PSEP1 86 Putative reductase Pput_4497 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=Pput_4497 PE=3 SV=1

+73

Accession Score Description
1 MATK_TRIAO 84 Maturase K OS=Trifolium albopurpureum GN=matK PE=3 SV=1

+74

Accession Score Description
1 FTSA_PSEAE 82 Cell division protein ftsA OS=Pseudomonas aeruginosa GN=ftsA PE=3 SV=2

+75

Accession Score Description
1 RL5_PSEE4 82 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

-76

Accession Score Description
1 ASPA_PSEAE 80 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1
Score Mass Matches Sequences emPAI
76.1 ASPA_PSEAE 80 51266 4 (3) 3 (2) 0.14
Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
581   393.2498 784.4850 784.4807 5.57 1 13 1.1 +2Score > 40 indicates identity
Score > 26 indicates homology
R.GVLELVR.E
962   472.3088 942.6030 942.5936 10.0 0 50 0.00038 +1Score > 33 indicates identity
Score > 28 indicates homology
U K.LVVALAMVK.Q
3456   736.7296 2207.1670 2207.1474 8.87 1 33 0.00093 +1Score > 37 indicates identity
Score > 15 indicates homology
U K.DLLGTLEVPADAYYGIQTLR.A
3458   1105.0872 2208.1598 2208.1314 12.9 1 46 0.0013 +1Score > 37 indicates identity
Score > 29 indicates homology
U K.DLLGTLEVPADAYYGIQTLR.A + Deamidated (NQ)

+77

Accession Score Description
1 TAL_PSEPG 80 Transaldolase OS=Pseudomonas putida (strain GB-1) GN=tal PE=3 SV=1

+78

Accession Score Description
1 THRC_PSEAE 79 Threonine synthase OS=Pseudomonas aeruginosa GN=thrC PE=3 SV=3

+79

Accession Score Description
1 ACP_PSEPK 78 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+80

Accession Score Description
1 PUR1_PSEAE 78 Amidophosphoribosyltransferase OS=Pseudomonas aeruginosa GN=purF PE=3 SV=3
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