MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 250)


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+71

Accession Score Description
1 CH10_PSEP1 87 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1

+72

Accession Score Description
1 Y4497_PSEP1 86 Putative reductase Pput_4497 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=Pput_4497 PE=3 SV=1

+73

Accession Score Description
1 MATK_TRIAO 84 Maturase K OS=Trifolium albopurpureum GN=matK PE=3 SV=1

+74

Accession Score Description
1 FTSA_PSEAE 82 Cell division protein ftsA OS=Pseudomonas aeruginosa GN=ftsA PE=3 SV=2

-75

Accession Score Description
1 RL5_PSEE4 82 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
Score Mass Matches Sequences emPAI
75.1 RL5_PSEE4 82 20385 5 (2) 4 (2) 0.24
50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1
4 samesets of RL5_PSEE4
RL5_PSEP1 82 20316 5 (2) 4 (2) 0.24
50S ribosomal protein L5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplE PE=3 SV=1
RL5_PSEPG 82 20316 5 (2) 4 (2) 0.24
50S ribosomal protein L5 OS=Pseudomonas putida (strain GB-1) GN=rplE PE=3 SV=1
RL5_PSEPK 82 20316 5 (2) 4 (2) 0.24
50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1
RL5_PSEPW 82 20399 5 (2) 4 (2) 0.24
50S ribosomal protein L5 OS=Pseudomonas putida (strain W619) GN=rplE PE=3 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
339   671.3760 670.3687 670.3650 5.61 1 31 0.21 +5Score > 36 indicates identity R.NEIAPK.L
806   441.7953 881.5760 881.5698 7.08 0 45 0.0018 +1Score > 30 indicates identity U R.LLAISLPR.V
1434 +1 581.3308 1160.6470 1160.6401 5.98 0 8 0.91 +1Score > 39 indicates identity
Score > 20 indicates homology
R.GLDITLTTTAR.S
1765   439.5963 1315.7671 1315.7612 4.44 0 63 4.9e-006 +1Score > 34 indicates identity
Score > 23 indicates homology
U K.ITGQKPVVTFAR.K

+76

Accession Score Description
1 ASPA_PSEAE 80 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

+77

Accession Score Description
1 TAL_PSEPG 80 Transaldolase OS=Pseudomonas putida (strain GB-1) GN=tal PE=3 SV=1

+78

Accession Score Description
1 THRC_PSEAE 79 Threonine synthase OS=Pseudomonas aeruginosa GN=thrC PE=3 SV=3

+79

Accession Score Description
1 ACP_PSEPK 78 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+80

Accession Score Description
1 PUR1_PSEAE 78 Amidophosphoribosyltransferase OS=Pseudomonas aeruginosa GN=purF PE=3 SV=3
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