MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 250)


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-71

Accession Score Description
1 CH10_PSEP1 87 10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1
Score Mass Matches Sequences emPAI
71.1 CH10_PSEP1 87 10217 6 (1) 5 (1) 0.24
10 kDa chaperonin OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=groS PE=3 SV=1
13 samesets of CH10_PSEP1
CH10_PSEPK 87 10217 6 (1) 5 (1) 0.24
10 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groS PE=3 SV=1
CH10_PSEST 87 10261 6 (1) 5 (1) 0.24
10 kDa chaperonin OS=Pseudomonas stutzeri GN=groS PE=3 SV=1
CH10_PSEU5 87 10259 6 (1) 5 (1) 0.24
10 kDa chaperonin OS=Pseudomonas stutzeri (strain A1501) GN=groS PE=3 SV=1
CH10_PSEE4 87 10245 4 (1) 4 (1) 0.24
10 kDa chaperonin OS=Pseudomonas entomophila (strain L48) GN=groS PE=3 SV=1
CH10_PSEPG 87 10260 4 (1) 4 (1) 0.24
10 kDa chaperonin OS=Pseudomonas putida (strain GB-1) GN=groS PE=3 SV=1
CH10_PSEPW 87 10247 4 (1) 4 (1) 0.24
10 kDa chaperonin OS=Pseudomonas putida (strain W619) GN=groS PE=3 SV=1
CH10_PSE14 87 10261 5 (1) 4 (1) 0.24
10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1
CH10_PSESM 87 10261 5 (1) 4 (1) 0.24
10 kDa chaperonin OS=Pseudomonas syringae pv. tomato GN=groS PE=3 SV=1
CH10_PSEU2 87 10261 5 (1) 4 (1) 0.24
10 kDa chaperonin OS=Pseudomonas syringae pv. syringae (strain B728a) GN=groS PE=3 SV=1
CH10_PSEF5 87 10245 5 (1) 4 (1) 0.24
10 kDa chaperonin OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=groS PE=3 SV=1
CH10_PSEPU 87 10274 3 (1) 3 (1) 0.23
10 kDa chaperonin OS=Pseudomonas putida GN=groS PE=3 SV=1
CH10_PSEPF 87 10214 3 (1) 3 (1) 0.24
10 kDa chaperonin OS=Pseudomonas fluorescens (strain Pf0-1) GN=groS PE=3 SV=1
CH10_PSEMY 87 10229 2 (1) 2 (1) 0.24
10 kDa chaperonin OS=Pseudomonas mendocina (strain ymp) GN=groS PE=3 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
854 +1 451.2422 900.4698 900.4665 3.73 1 38 0.11 +1Score > 42 indicates identity
Score > 41 indicates homology
R.VLDNGEVR.A
869   302.8483 905.5231 905.5195 3.93 0 29 0.12 +1Score > 38 indicates identity
Score > 32 indicates homology
K.LRPLHDR.V
964   472.7638 943.5130 943.5087 4.59 1 39 0.11 +1Score > 42 indicates identity R.GEVVAVGTGR.V
1844   677.8566 1353.6986 1353.6929 4.26 0 87 1.2e-007 +1Score > 40 indicates identity
Score > 30 indicates homology
U K.VVFGPYSGSNTVK.V
2462   546.9734 1637.8984 1637.8737 15.1 1 16 0.38 +1Score > 37 indicates identity
Score > 25 indicates homology
K.TAGGIVLPGSAAEKPNR.G + Deamidated (NQ)

+72

Accession Score Description
1 Y4497_PSEP1 86 Putative reductase Pput_4497 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=Pput_4497 PE=3 SV=1

+73

Accession Score Description
1 MATK_TRIAO 84 Maturase K OS=Trifolium albopurpureum GN=matK PE=3 SV=1

+74

Accession Score Description
1 FTSA_PSEAE 82 Cell division protein ftsA OS=Pseudomonas aeruginosa GN=ftsA PE=3 SV=2

+75

Accession Score Description
1 RL5_PSEE4 82 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+76

Accession Score Description
1 ASPA_PSEAE 80 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

+77

Accession Score Description
1 TAL_PSEPG 80 Transaldolase OS=Pseudomonas putida (strain GB-1) GN=tal PE=3 SV=1

+78

Accession Score Description
1 THRC_PSEAE 79 Threonine synthase OS=Pseudomonas aeruginosa GN=thrC PE=3 SV=3

+79

Accession Score Description
1 ACP_PSEPK 78 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+80

Accession Score Description
1 PUR1_PSEAE 78 Amidophosphoribosyltransferase OS=Pseudomonas aeruginosa GN=purF PE=3 SV=3
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