MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1177 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 5 | EFTU_CARRP | 73 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1045 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
| 3 | EFTU_BACAA | 271 | Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1 | |
| 4 | EFTU_ANATD | 83 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 525 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 5 | ATPG_RHOP5 | 40 | description | |
| 3 | ATPB_LEGPA | 167 | ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1 | |
| 2 | CH60_PSEPK | 473 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 6 | CH602_PROMS | 39 | 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1 | |
| 4 | CH602_SINMW | 59 | 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPG | 522 | ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 112 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 463 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 391 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPG | 350 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1 | |
| 2 | RPOC_PSEA7 | 244 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1 | |
| 3 | FTSZ_PSEPK | 95 | Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3 | |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 349 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 7.1 | DLDH2_PSEPU |
349 | 50093 | 17 (11) | 12 (8) | 0.56 | |
| Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 | |||||||
17 peptide matches (16 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 143 |
487.3268 | 486.3195 | 486.3166 | 6.07 | 0 | 25 | 1.5 | 2Score > 46 indicates identity Score > 40 indicates homology |
K.AGIVK.N | ||
| 157 |
501.3413 | 500.3340 | 500.3322 | 3.62 | 0 | 34 | 0.074 | 1Score > 42 indicates identity Score > 35 indicates homology |
K.LLAGK.K | ||
| 253 |
312.1746 | 622.3346 | 622.3326 | 3.25 | 0 | 19 | 0.61 | 1Score > 41 indicates identity Score > 29 indicates homology |
K.ITFDK.L | ||
| 398 |
700.4379 | 699.4306 | 699.4279 | 3.89 | 0 | 50 | 0.0079 | 1Score > 41 indicates identity |
U | K.AAQLGLK.T | |
| 399 |
350.7226 | 699.4306 | 699.4279 | 3.93 | 0 | 28 | 0.33 | 2Score > 41 indicates identity Score > 36 indicates homology |
U | K.AAQLGLK.T | |
| 452 |
364.2466 | 726.4786 | 726.4752 | 4.74 | 0 | 35 | 0.042 | 1Score > 34 indicates identity Score > 34 indicates homology |
U | K.LIVAVGR.R | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_3_20250714120051.rawScore > 42 indicates identity Score > 36 indicates homology |
|||||||||||
| 897 |
460.2500 | 918.4854 | 918.4811 | 4.77 | 0 | 36 | 0.06 | 1Score > 42 indicates identity Score > 36 indicates homology |
U | K.ALLDSSWK.Y | |
| -18.2 | 0 | 21 | 1.6 | 2 | IAIDSVSSK | ||||||
| -11.1 | 0 | 12 | 15 | 3 | AAMAASILR + Oxidation (M) | ||||||
| 1.12 | 0 | 10 | 22 | 4 | ALINNMVK + Deamidated (NQ); Oxidation (M) | ||||||
| 1.12 | 0 | 10 | 22 | 4 | ALINNVMK + Deamidated (NQ); Oxidation (M) | ||||||
| 1.10 | 0 | 10 | 22 | 4 | ALLDQMTK | ||||||
| -7.45 | 0 | 10 | 22 | 4 | ALLNQGFR + Deamidated (NQ) | ||||||
| 9.16 | 0 | 10 | 24 | 8 | VSITNASAR + Deamidated (NQ) | ||||||
| 4.77 | 0 | 9 | 25 | 9 | LALAGQFAE | ||||||
| -11.1 | 0 | 9 | 26 | 10 | ALLSAMAAR + Oxidation (M) | ||||||
| 1332 |
560.8253 | 1119.6360 | 1119.6288 | 6.46 | 0 | 33 | 0.0061 | 1Score > 38 indicates identity Score > 23 indicates homology |
U | K.NLTGGVATLFK.A | |
| 1334 |
561.3202 | 1120.6258 | 1120.6128 | 11.6 | 0 | 9 | 3.7 | 3Score > 38 indicates identity Score > 27 indicates homology |
U | K.NLTGGVATLFK.A + Deamidated (NQ) | |
| 1445 |
584.8097 | 1167.6048 | 1167.5996 | 4.46 | 0 | 59 | 3.7e-005 | 1Score > 40 indicates identity Score > 27 indicates homology |
U | K.ANGVTSIQGHGK.L | |
| 1446 |
390.2102 | 1167.6088 | 1167.5996 | 7.82 | 0 | 35 | 0.0051 | 1Score > 40 indicates identity Score > 25 indicates homology |
U | K.ANGVTSIQGHGK.L | |
| 1478 |
591.2869 | 1180.5592 | 1180.5547 | 3.89 | 0 | 63 | 2.3e-005 | 1Score > 41 indicates identity Score > 29 indicates homology |
U | R.AMAANDTGGFVK.V | |
| 2884 |
1 |
934.0504 | 1866.0862 | 1866.0727 | 7.25 | 1 | 92 | 3.3e-008 | 1Score > 30 indicates identity |
U | R.LGVIGAGVIGLELGSVWAR.L |
| 2886 |
623.0419 | 1866.1039 | 1866.0727 | 16.7 | 1 | 76 | 8.3e-008 | 1Score > 28 indicates identity Score > 18 indicates homology |
U | R.LGVIGAGVIGLELGSVWAR.L | |
| 3218 |
1046.0964 | 2090.1782 | 2090.1412 | 17.7 | 1 | 48 | 0.0014 | 1Score > 32 indicates identity |
U | M.TQKFDVVVIGAGPGGYVAAIK.A + Deamidated (NQ) | |
| 4000 |
882.7962 | 2645.3668 | 2645.3199 | 17.7 | 1 | 50 | 4.1e-005 | 1Score > 37 indicates identity Score > 19 indicates homology |
U | K.AQMNYDLIPSVIYTHPEIAWVGK.T + Deamidated (NQ) | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| DLDH2_PSEAE |
248 | 50362 | 7.1 | ||
| Dihydrolipoamide dehydrogenase OS=Pseudomonas aeruginosa GN=lpdG PE=3 SV=1 | |||||
| 1 sameset of DLDH2_PSEAE | |||||
| DLDH_PSEFL |
248 | 50348 | |||
| Dihydrolipoyl dehydrogenase OS=Pseudomonas fluorescens GN=lpd PE=1 SV=3 | |||||
| DLDH_AZOVI |
207 | 49707 | 7.1 | ||
| Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1 | |||||
| DLDH2_ARATH |
50 | 54237 | 7.1 | ||
| Dihydrolipoyl dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=LPD2 PE=1 SV=1 | |||||
| 3 samesets of DLDH2_ARATH | |||||
| DLDH_SOLTU |
50 | 3910 | |||
| Dihydrolipoyl dehydrogenase (Fragment) OS=Solanum tuberosum PE=1 SV=1 | |||||
| DLDH_TRYBB |
50 | 50815 | |||
| Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei brucei PE=3 SV=1 | |||||
| DLDH2_BACSU |
50 | 50632 | |||
| Dihydrolipoyl dehydrogenase OS=Bacillus subtilis GN=bfmBC PE=3 SV=1 | |||||
| DFX_METTH |
35 | 14264 | 7.1 | ||
| Desulfoferrodoxin homolog OS=Methanobacterium thermoautotrophicum GN=MTH_757 PE=3 SV=1 | |||||
| 6 samesets of DFX_METTH | |||||
| DLDH_THESC |
35 | 48589 | |||
| Dihydrolipoyl dehydrogenase OS=Thermus scotoductus GN=lpd PE=1 SV=2 | |||||
| GLMU_DECAR |
35 | 48067 | |||
| Bifunctional protein glmU OS=Dechloromonas aromatica (strain RCB) GN=glmU PE=3 SV=1 | |||||
| RPPH_ACICJ |
35 | 18342 | |||
| RNA pyrophosphohydrolase OS=Acidiphilium cryptum (strain JF-5) GN=rppH PE=3 SV=1 | |||||
| YNF1_RHOCA |
35 | 27985 | |||
| Uncharacterized 27.7 kDa protein in nifB 3'region OS=Rhodobacter capsulatus PE=4 SV=1 | |||||
| DLDH3_HALMA |
35 | 50375 | |||
| Dihydrolipoyl dehydrogenase 3 OS=Haloarcula marismortui GN=lpdA3 PE=3 SV=1 | |||||
| HSE1_SCHPO |
35 | 42699 | |||
| Class E vacuolar protein-sorting machinery protein hse1 OS=Schizosaccharomyces pombe GN=hse1 PE=2 SV=1 | |||||
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 327 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 321 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 313 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
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