MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 250)


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+61

Accession Score Description
1 ODP1_PSEAE 97 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+62

Accession Score Description
1 RL10_PSEPG 97 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+63

Accession Score Description
1 GLPK_PSEPG 97 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

+64

Accession Score Description
1 KATG_PSEPK 97 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

+65

Accession Score Description
1 SYD_PSEPG 96 Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1

-66

Accession Score Description
Family member distances as a dendrogram 1 G3P1_BACCE 95 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Bacillus cereus GN=gap1 PE=1 SV=3
2 G3P2_ANASP 43 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena sp. (strain PCC 7120) GN=gap2 PE=1 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
66.1 G3P1_BACCE 95 35974 5 (3) 5 (3) 0.20
Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Bacillus cereus GN=gap1 PE=1 SV=3
66.2 G3P2_ANASP 43 37115 2 (2) 2 (2) 0.13
Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena sp. (strain PCC 7120) GN=gap2 PE=1 SV=1
1 sameset of G3P2_ANASP
G3P2_ANAVT 43 37081 2 (2) 2 (2) 0.13
Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=gap2 PE=3 SV=2

+6 peptide matches (6 non-duplicate, 0 duplicate)

2 subsets and intersections (15 subset proteins in total)

Score Mass Subset of
G3P1_ANASP 37 36957 66.1
Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Anabaena sp. (strain PCC 7120) GN=gap1 PE=1 SV=3
4 samesets of G3P1_ANASP
G3P_BACST 37 36167
Glyceraldehyde-3-phosphate dehydrogenase OS=Bacillus stearothermophilus GN=gap PE=1 SV=5
G3P1_ANAVT 37 36871
Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=gap1 PE=3 SV=4
G3P_CORGL 37 36194
Glyceraldehyde-3-phosphate dehydrogenase OS=Corynebacterium glutamicum GN=gap PE=1 SV=2
G3P_ZYMMO 37 36251
Glyceraldehyde-3-phosphate dehydrogenase OS=Zymomonas mobilis GN=gap PE=3 SV=1
G3P2_BACSU 28 37681 66.2
Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Bacillus subtilis GN=gapB PE=1 SV=1
9 samesets of G3P2_BACSU
G3P2_SYNY3 28 36775
Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Synechocystis sp. (strain PCC 6803) GN=gap2 PE=1 SV=3
G3PA_ARATH 28 42748
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Arabidopsis thaliana GN=GAPA PE=1 SV=3
G3PA_CHOCR 28 44830
Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic OS=Chondrus crispus GN=GAPA PE=2 SV=1
G3PA_GRAVE 28 44708
Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic OS=Gracilaria verrucosa GN=GAPA PE=2 SV=1
G3PA_MAIZE 28 43182
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Zea mays GN=GAPA PE=2 SV=1
G3PA_PEA 28 43597
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Pisum sativum GN=GAPA PE=2 SV=2
G3PA_SINAL 28 25315
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (Fragment) OS=Sinapis alba GN=GAPA PE=2 SV=1
G3PA_SPIOL 28 43338
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Spinacia oleracea GN=GAPA PE=1 SV=2
G3PA_TOBAC 28 42122
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (Fragment) OS=Nicotiana tabacum GN=GAPA PE=2 SV=1

+67

Accession Score Description
1 ASSY_PSEPG 94 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+68

Accession Score Description
1 RL7_PSEP1 91 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+69

Accession Score Description
1 SYT_PSEPG 90 Threonyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=thrS PE=3 SV=1

+70

Accession Score Description
1 OADC_PSEPG 89 Oxaloacetate decarboxylase OS=Pseudomonas putida (strain GB-1) GN=PputGB1_4424 PE=3 SV=1
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