MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 250)


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+61

Accession Score Description
1 ODP1_PSEAE 97 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+62

Accession Score Description
1 RL10_PSEPG 97 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+63

Accession Score Description
1 GLPK_PSEPG 97 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

+64

Accession Score Description
1 KATG_PSEPK 97 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

-65

Accession Score Description
1 SYD_PSEPG 96 Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1
Score Mass Matches Sequences emPAI
65.1 SYD_PSEPG 96 66894 6 (3) 6 (3) 0.11
Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1
4 samesets of SYD_PSEPG
SYD_PSEPK 96 66925 6 (3) 6 (3) 0.11
Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=aspS PE=3 SV=1
SYD_PSEP1 96 66925 6 (3) 6 (3) 0.11
Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=aspS PE=3 SV=1
SYD_PSEE4 96 66880 6 (3) 6 (3) 0.11
Aspartyl-tRNA synthetase OS=Pseudomonas entomophila (strain L48) GN=aspS PE=3 SV=1
SYD_PSEPW 96 66849 5 (3) 5 (3) 0.11
Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain W619) GN=aspS PE=3 SV=1

-6 peptide matches (6 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
328   332.1961 662.3776 662.3752 3.77 0 31 0.086 +1Score > 39 indicates identity
Score > 33 indicates homology
R.FIDLR.R
1165   512.3030 1022.5914 1022.5801 11.1 0 48 0.0027 +1Score > 35 indicates identity U K.FGFLLDALK.F
1340   562.3087 1122.6028 1122.5921 9.59 1 30 0.0026 +1Score > 39 indicates identity
Score > 16 indicates homology
U R.SEYVVQITGK.V
2126   748.4009 1494.7872 1494.7790 5.51 1 14 0.19 +1Score > 39 indicates identity
Score > 19 indicates homology
U K.NIPEANLNNILDR.V
2155   755.3870 1508.7594 1508.7511 5.50 0 69 2.8e-006 +1Score > 41 indicates identity
Score > 26 indicates homology
U R.VGAVDGDIVFFGADK.F
2159   504.5984 1510.7734 1510.7681 3.48 0 22 0.085 +1Score > 40 indicates identity
Score > 24 indicates homology
K.FGAPPHGGLAFGLDR.L

2 subsets and intersections (123 subset proteins in total)

Score Mass Subset of
SYD_AZOVD 87 66329 65.1
Aspartyl-tRNA synthetase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=aspS PE=3 SV=1
10 samesets of SYD_AZOVD
SYD_PSESM 87 66595
Aspartyl-tRNA synthetase OS=Pseudomonas syringae pv. tomato GN=aspS PE=3 SV=1
SYD_PSEU2 87 66583
Aspartyl-tRNA synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=aspS PE=3 SV=1
SYD_PSE14 87 66526
Aspartyl-tRNA synthetase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=aspS PE=3 SV=1
SYD_PSEAB 87 66451
Aspartyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=aspS PE=3 SV=1
SYD_PSEAE 87 66451
Aspartyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=aspS PE=3 SV=2
SYD_PSEFS 87 66562
Aspartyl-tRNA synthetase OS=Pseudomonas fluorescens (strain SBW25) GN=aspS PE=3 SV=1
SYD_PSEMY 87 66405
Aspartyl-tRNA synthetase OS=Pseudomonas mendocina (strain ymp) GN=aspS PE=3 SV=1
SYD_PSEPF 87 66553
Aspartyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=aspS PE=3 SV=1
SYD_PSEA8 87 66451
Aspartyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain LESB58) GN=aspS PE=3 SV=1
SYD_PSEF5 87 66639
Aspartyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=aspS PE=3 SV=1
SYD_ACTP2 48 66995 65.1
Aspartyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=aspS PE=3 SV=1
+111 samesets of SYD_ACTP2

+66

Accession Score Description
Family member distances as a dendrogram 1 G3P1_BACCE 95 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Bacillus cereus GN=gap1 PE=1 SV=3
2 G3P2_ANASP 43 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena sp. (strain PCC 7120) GN=gap2 PE=1 SV=1

+67

Accession Score Description
1 ASSY_PSEPG 94 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+68

Accession Score Description
1 RL7_PSEP1 91 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+69

Accession Score Description
1 SYT_PSEPG 90 Threonyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=thrS PE=3 SV=1

+70

Accession Score Description
1 OADC_PSEPG 89 Oxaloacetate decarboxylase OS=Pseudomonas putida (strain GB-1) GN=PputGB1_4424 PE=3 SV=1
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