MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 250)


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+61

Accession Score Description
1 ODP1_PSEAE 97 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+62

Accession Score Description
1 RL10_PSEPG 97 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+63

Accession Score Description
1 GLPK_PSEPG 97 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

-64

Accession Score Description
1 KATG_PSEPK 97 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1
Score Mass Matches Sequences emPAI
64.1 KATG_PSEPK 97 82068 5 (4) 4 (3) 0.12
Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1
1 sameset of KATG_PSEPK
KATG_PSEP1 97 82012 5 (4) 4 (3) 0.12
Catalase-peroxidase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=katG PE=3 SV=1

-5 peptide matches (5 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2528   557.3354 1668.9844 1668.9662 10.9 1 34 0.026 +1Score > 31 indicates identity U K.VSLADLIVLAGTAAVEK.A
2529   835.4995 1668.9844 1668.9662 11.0 1 35 0.023 +1Score > 31 indicates identity U K.VSLADLIVLAGTAAVEK.A
2580   851.9341 1701.8536 1701.8362 10.2 0 53 9.5e-005 +1Score > 40 indicates identity
Score > 25 indicates homology
U R.FAPLNSWPDNVSLDK.A
3641   775.0889 2322.2449 2322.2220 9.87 1 11 0.57 +1Score > 35 indicates identity
Score > 22 indicates homology
U R.ILASGLSVGELVSTAWASASTFR.G
4409   1018.4926 3052.4560 3052.4066 16.2 0 56 1.5e-005 +1Score > 37 indicates identity
Score > 21 indicates homology
U K.DLTALMTDSQDWWPADFGHYGPLFIR.M + Deamidated (NQ)

3 subsets and intersections (87 subset proteins in total)

Score Mass Subset of
KATG_PSEE4 86 81089 64.1
Catalase-peroxidase OS=Pseudomonas entomophila (strain L48) GN=katG PE=3 SV=1
1 sameset of KATG_PSEE4
KATG_PSEPG 86 81707
Catalase-peroxidase OS=Pseudomonas putida (strain GB-1) GN=katG PE=3 SV=1
KATG1_BURCA 53 79222 64.1
Catalase-peroxidase 1 OS=Burkholderia cenocepacia (strain AU 1054) GN=katG1 PE=3 SV=1
+83 samesets of KATG1_BURCA
KATG_PYRTR 38 84619 64.1
Catalase-peroxidase OS=Pyrenophora tritici-repentis (strain Pt-1C-BFP) GN=katG PE=3 SV=1

+65

Accession Score Description
1 SYD_PSEPG 96 Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1

+66

Accession Score Description
Family member distances as a dendrogram 1 G3P1_BACCE 95 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Bacillus cereus GN=gap1 PE=1 SV=3
2 G3P2_ANASP 43 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena sp. (strain PCC 7120) GN=gap2 PE=1 SV=1

+67

Accession Score Description
1 ASSY_PSEPG 94 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+68

Accession Score Description
1 RL7_PSEP1 91 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+69

Accession Score Description
1 SYT_PSEPG 90 Threonyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=thrS PE=3 SV=1

+70

Accession Score Description
1 OADC_PSEPG 89 Oxaloacetate decarboxylase OS=Pseudomonas putida (strain GB-1) GN=PputGB1_4424 PE=3 SV=1
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