MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 250)


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+61

Accession Score Description
1 ODP1_PSEAE 97 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

-62

Accession Score Description
1 RL10_PSEPG 97 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
Score Mass Matches Sequences emPAI
62.1 RL10_PSEPG 97 17676 3 (2) 3 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1
3 samesets of RL10_PSEPG
RL10_PSEPK 97 17676 3 (2) 3 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain KT2440) GN=rplJ PE=3 SV=1
RL10_PSEPW 97 17648 3 (2) 3 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas putida (strain W619) GN=rplJ PE=3 SV=1
RL10_PSEE4 97 17602 2 (2) 2 (2) 0.28
50S ribosomal protein L10 OS=Pseudomonas entomophila (strain L48) GN=rplJ PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1242   536.3142 1070.6138 1070.6084 5.09 0 56 0.0014 +1Score > 40 indicates identity U K.VALSAVVADAR.G
1365   567.8145 1133.6144 1133.6114 2.66 0 24 0.14 +1Score > 40 indicates identity
Score > 28 indicates homology
R.LMSVIQGATSK.L
2597   570.2999 1707.8779 1707.8692 5.05 1 74 6.9e-007 +1Score > 40 indicates identity
Score > 24 indicates homology
U K.GPTLIAFSNEHPGAAAR.L

1 subset or intersection (7 subset proteins in total)

Score Mass Subset of
RL10_AZOVD 74 17534 62.1
50S ribosomal protein L10 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplJ PE=3 SV=1
6 samesets of RL10_AZOVD
RL10_PSEA7 74 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain PA7) GN=rplJ PE=3 SV=1
RL10_PSEA8 74 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplJ PE=3 SV=1
RL10_PSEAB 74 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplJ PE=3 SV=1
RL10_PSEAE 74 17623
50S ribosomal protein L10 OS=Pseudomonas aeruginosa GN=rplJ PE=3 SV=1
RL10_PSEMY 74 17574
50S ribosomal protein L10 OS=Pseudomonas mendocina (strain ymp) GN=rplJ PE=3 SV=1
RL10_PSEU5 74 17514
50S ribosomal protein L10 OS=Pseudomonas stutzeri (strain A1501) GN=rplJ PE=3 SV=1

+63

Accession Score Description
1 GLPK_PSEPG 97 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

+64

Accession Score Description
1 KATG_PSEPK 97 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

+65

Accession Score Description
1 SYD_PSEPG 96 Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1

+66

Accession Score Description
Family member distances as a dendrogram 1 G3P1_BACCE 95 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Bacillus cereus GN=gap1 PE=1 SV=3
2 G3P2_ANASP 43 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena sp. (strain PCC 7120) GN=gap2 PE=1 SV=1

+67

Accession Score Description
1 ASSY_PSEPG 94 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+68

Accession Score Description
1 RL7_PSEP1 91 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+69

Accession Score Description
1 SYT_PSEPG 90 Threonyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=thrS PE=3 SV=1

+70

Accession Score Description
1 OADC_PSEPG 89 Oxaloacetate decarboxylase OS=Pseudomonas putida (strain GB-1) GN=PputGB1_4424 PE=3 SV=1
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