MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 250)


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-61

Accession Score Description
1 ODP1_PSEAE 97 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2
Score Mass Matches Sequences emPAI
61.1 ODP1_PSEAE 97 99843 9 (4) 8 (3) 0.09
Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
309   326.1775 650.3404 650.3387 2.62 1 19 0.75 +1Score > 44 indicates identity
Score > 30 indicates homology
K.YLAER.R
312   328.6912 655.3678 655.3653 3.85 0 15 1.3 +9Score > 37 indicates identity
Score > 29 indicates homology
R.LDGPVR.G
379   345.2034 688.3922 688.3908 2.09 0 19 1.4 +2Score > 41 indicates identity
Score > 33 indicates homology
U R.GFIPAGK.Q
489   372.7069 743.3992 743.3966 3.53 0 17 1.2 +2Score > 41 indicates identity
Score > 30 indicates homology
U R.QWVPSK.E
523   380.2048 758.3950 758.3923 3.68 1 30 0.3 +1Score > 44 indicates identity
Score > 37 indicates homology
U R.DGLAVER.W
936   466.2657 930.5168 930.5134 3.66 1 52 0.0051 +1Score > 42 indicates identity U K.KVDVDSLR.A
1368   379.8731 1136.5975 1136.5938 3.22 1 42 0.0027 +1Score > 40 indicates identity
Score > 29 indicates homology
U R.NTIPVTHEAR.M
1369   569.3066 1136.5986 1136.5938 4.26 1 45 0.0036 +1Score > 40 indicates identity
Score > 33 indicates homology
U R.NTIPVTHEAR.M
1592   616.3093 1230.6040 1230.5993 3.87 0 61 8.8e-005 +1Score > 41 indicates identity
Score > 33 indicates homology
U R.IGDLAWAAGDSR.A

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
SPRT_VIBSL 52 19585 61.1
Protein sprT OS=Vibrio splendidus (strain LGP32) GN=sprT PE=3 SV=1

+62

Accession Score Description
1 RL10_PSEPG 97 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+63

Accession Score Description
1 GLPK_PSEPG 97 Glycerol kinase OS=Pseudomonas putida (strain GB-1) GN=glpK PE=3 SV=1

+64

Accession Score Description
1 KATG_PSEPK 97 Catalase-peroxidase OS=Pseudomonas putida (strain KT2440) GN=katG PE=3 SV=1

+65

Accession Score Description
1 SYD_PSEPG 96 Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1

+66

Accession Score Description
Family member distances as a dendrogram 1 G3P1_BACCE 95 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Bacillus cereus GN=gap1 PE=1 SV=3
2 G3P2_ANASP 43 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Anabaena sp. (strain PCC 7120) GN=gap2 PE=1 SV=1

+67

Accession Score Description
1 ASSY_PSEPG 94 Argininosuccinate synthase OS=Pseudomonas putida (strain GB-1) GN=argG PE=3 SV=1

+68

Accession Score Description
1 RL7_PSEP1 91 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+69

Accession Score Description
1 SYT_PSEPG 90 Threonyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=thrS PE=3 SV=1

+70

Accession Score Description
1 OADC_PSEPG 89 Oxaloacetate decarboxylase OS=Pseudomonas putida (strain GB-1) GN=PputGB1_4424 PE=3 SV=1
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