MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita3 sp
MS data file : PRT1270_T-BRSC_3_20250714120051.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:29 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 5,012

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 250)

Page: 1 2 3 4 5 6 … 25 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1177 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
5 EFTU_CARRP 73 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1045 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 EFTU_BACAA 271 Elongation factor Tu OS=Bacillus anthracis (strain A0248) GN=tuf PE=3 SV=1
4 EFTU_ANATD 83 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 525 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
5 ATPG_RHOP5 40 description
3 ATPB_LEGPA 167 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
2 CH60_PSEPK 473 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
6 CH602_PROMS 39 60 kDa chaperonin 2 OS=Prochlorococcus marinus (strain AS9601) GN=groL2 PE=3 SV=1
4 CH602_SINMW 59 60 kDa chaperonin 2 OS=Sinorhizobium medicae (strain WSM419) GN=groL2 PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPG 522 ATP synthase subunit alpha OS=Pseudomonas putida (strain GB-1) GN=atpA PE=3 SV=1
2 ATPA_RICAH 112 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1

+4

Accession Score Description
1 ARCA_PSEPK 463 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+5

Accession Score Description
1 OTCC_PSEPK 391 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-6

Accession Score Description
Family member distances as a dendrogram 1 RPOC_PSEPG 350 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
2 RPOC_PSEA7 244 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1
3 FTSZ_PSEPK 95 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
Cut threshold

Score Mass Matches Sequences emPAI
RPOC_PSEPG 350 155435 22 (12) 21 (11) 0.19
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1
4 samesets of RPOC_PSEPG
RPOC_PSEPK 350 155386 22 (12) 21 (11) 0.19
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1
RPOC_PSEPW 350 155341 22 (12) 21 (11) 0.19
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain W619) GN=rpoC PE=3 SV=1
RPOC_PSEP1 350 155358 22 (12) 21 (11) 0.19
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1
RPOC_PSEE4 350 155376 22 (12) 21 (11) 0.19
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
RPOC_PSEA7 244 154957 15 (7) 15 (7) 0.11
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain PA7) GN=rpoC PE=3 SV=1
3 samesets of RPOC_PSEA7
RPOC_PSEU5 244 155574 16 (7) 16 (7) 0.11
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas stutzeri (strain A1501) GN=rpoC PE=3 SV=1
RPOC_PSEAB 244 154971 15 (7) 15 (7) 0.11
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoC PE=3 SV=1
RPOC_PSEAE 244 154971 15 (7) 15 (7) 0.11
DNA-directed RNA polymerase subunit beta' OS=Pseudomonas aeruginosa GN=rpoC PE=3 SV=1
FTSZ_PSEPK 95 41918 6 (4) 6 (4) 0.24
Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3

-29 peptide matches (29 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
251   311.1719 620.3292 620.3282 1.69 0 15 8.7 +8Score > 42 indicates identity
Score > 37 indicates homology		 X X E.FDAIR.I
417   352.2228 702.4310 702.4276 4.93 0 10 6.2 +7Score > 38 indicates identity
Score > 31 indicates homology		 U X R.GLATTIK.A
487   372.2563 742.4980 742.4953 3.74 0 25 0.082 +1Score > 32 indicates identity
Score > 27 indicates homology		 U X X R.VLLGITK.A
521   379.2648 756.5150 756.5109 5.47 0 28 0.03 +1Score > 26 indicates identity U X K.LLTILGK.D
758   430.7503 859.4860 859.4803 6.63 0 27 0.11 +1Score > 41 indicates identity
Score > 29 indicates homology		 U X X K.VIDLWSK.A
783   436.2875 870.5604 870.5538 7.60 0 39 0.014 +1Score > 33 indicates identity U X R.LLGVSALAK.Y
891   458.7790 915.5434 915.5389 4.96 1 62 0.00027 +1Score > 39 indicates identity U X X R.KGLADTALK.T
899   460.7472 919.4798 919.4763 3.84 1 26 0.59 +1Score > 42 indicates identity
Score > 36 indicates homology		 U X X R.VADLFEAR.R
1000   479.2991 956.5836 956.5906 -7.26 1 33 0.19 +1Score > 39 indicates identity U X X M.KDLLNLLK.N + Deamidated (NQ)
1050   493.7693 985.5240 985.5193 4.86 0 56 0.00064 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X K.ADDVLAGAVR.G
1053   494.7894 987.5642 987.5601 4.24 1 51 0.0037 +1Score > 39 indicates identity U X X R.VLTEAAVTGK.R
1091   501.2755 1000.5364 1000.5302 6.29 0 75 5.6e-006 +1Score > 42 indicates identity
Score > 35 indicates homology		 U X R.ADGNLVAVSR.S
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_3_20250714120051.raw

Score > 42 indicates identity

Score > 34 indicates homology
1103   503.2665 1004.5184 1004.5138 4.59 0 62 9.4e-005 -1Score > 42 indicates identity
Score > 34 indicates homology		 U X R.TSAADSVQVK.N
4.63 1 18 2.3 2 TSQKNNALK   + 2 Deamidated (NQ)
4.63 1 17 3.1 3 TSQKNNALK   + 2 Deamidated (NQ)
4.63 1 16 3.6 4 TSQKNNALK   + 2 Deamidated (NQ)
4.60 0 16 3.7 5 TSAAAVQGTAK   + Deamidated (NQ)
11.1 0 15 4.8 6 LGSVGMAQAR   + Oxidation (M)
-13.9 0 15 5 7 STAMLNQIK  
14.4 0 13 6.6 8 TSGSIWQAR  
4.63 0 13 7.7 9 NANLSASSLK   + Deamidated (NQ)
-10.6 0 13 7.7 10 IQNQFNLK   + Deamidated (NQ)
1141   339.5154 1015.5244 1015.5199 4.38 0 19 1.3 +2Score > 42 indicates identity
Score > 33 indicates homology		 X X R.TFHIGGAASR.T
1157   511.7835 1021.5524 1021.5444 7.89 0 7 1.1 +2Score > 39 indicates identity
Score > 20 indicates homology		 U X K.DASLLSAFAK.A
1197   521.8202 1041.6258 1041.6183 7.29 0 31 0.067 +1Score > 38 indicates identity
Score > 32 indicates homology		 U X X R.SVITVGPTLR.L
1288   549.2921 1096.5696 1096.5625 6.50 1 62 0.0003 +1Score > 39 indicates identity U X K.GLGAGANPEVGR.Q
1353   565.8461 1129.6776 1129.6707 6.16 0 44 0.00075 +1Score > 34 indicates identity
Score > 25 indicates homology		 U X R.TILQLGTGVTK.G
1761   657.8250 1313.6354 1313.6252 7.83 0 69 1.2e-005 +1Score > 41 indicates identity
Score > 32 indicates homology		 U X X R.FATSDLNDLYR.R
1817   446.6015 1336.7827 1336.7755 5.40 1 44 0.0018 +1Score > 34 indicates identity
Score > 29 indicates homology		 U X X R.YKLPYGAVISVK.E
1961   706.3677 1410.7208 1410.7143 4.63 1 86 1.3e-006 +1Score > 40 indicates identity U X X K.YIVNEIQDVYR.L
2109   495.6019 1483.7839 1483.7783 3.75 1 75 8.7e-006 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X X R.LIPAGTGLAYHSER.K
2172   757.4415 1512.8684 1512.8552 8.78 1 6 0.7 +1Score > 35 indicates identity
Score > 17 indicates homology		 X X R.LGIQAFEPVLIEGK.A
2591   569.6185 1705.8337 1705.8271 3.84 1 69 1.4e-005 +1Score > 41 indicates identity
Score > 32 indicates homology		 U X R.GEVISDGPSNPHDILR.L + Deamidated (NQ)
2591   569.6185 1705.8337 1705.8271 3.83 1 67 2e-005 +2Score > 41 indicates identity
Score > 32 indicates homology		 U X R.GEVISDGPSDPHDILR.L
2748   593.3205 1776.9397 1776.9159 13.4 1 42 0.0017 +1Score > 39 indicates identity
Score > 27 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
2749   889.4778 1776.9410 1776.9159 14.2 1 45 0.00049 +1Score > 39 indicates identity
Score > 25 indicates homology		 U X R.EGLSVLQYFISTHGAR.K
3599   767.4296 2299.2670 2299.2286 16.7 0 23 0.75 +1Score > 34 indicates identity U X R.ALLFQVVPAGLPYDVVNQPMK.K + Deamidated (NQ)
4423   1027.5001 3079.4785 3079.5363 -18.8 1 0 1.1 +4Score > 37 indicates identity
Score > 13 indicates homology		 U X R.NQAHAGAAAAAKLNPQDDLDYLDIPAFLR.R + Deamidated (NQ)

+18 subsets and intersections (638 subset proteins in total)

+7

Accession Score Description
1 DLDH2_PSEPU 349 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+8

Accession Score Description
1 ADHP_ECOLI 327 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+9

Accession Score Description
1 ACON2_PSEAE 321 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+10

Accession Score Description
1 SUCC_PSEPK 313 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
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