MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPG | 110 | Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSE14 | 110 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEE4 | 107 | 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LEU1_PSEP1 | 103 | 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CISY_PSEAE | 103 | Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEE4 | 102 | Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SODF_PSEPK | 99 | Superoxide dismutase [Fe] OS=Pseudomonas putida (strain KT2440) GN=sodB PE=3 SV=3 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 100 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 58.1 | PORF_PSEAE |
100 | 37844 | 12 (4) | 4 (2) | 0.19 | |
| Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |||||||
12 peptide matches (6 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 335 |
2 |
336.1565 | 670.2984 | 670.2962 | 3.28 | 0 | 13 | 0.43 | 1Score > 37 indicates identity Score > 22 indicates homology |
K.FDFDK.S | |
| 338 |
671.3061 | 670.2988 | 670.2962 | 3.84 | 0 | 27 | 0.32 | 1Score > 37 indicates identity Score > 34 indicates homology |
K.FDFDK.S | ||
| 411 |
1 |
351.7063 | 701.3980 | 701.3959 | 2.99 | 0 | 27 | 1.2 | 2Score > 43 indicates identity Score > 41 indicates homology |
U | R.VQLDVK.F + Deamidated (NQ) |
| 696 |
2 |
419.7119 | 837.4092 | 837.4055 | 4.53 | 0 | 29 | 0.19 | 1Score > 40 indicates identity Score > 34 indicates homology |
U | K.NLADFMK.Q |
| 698 |
838.4172 | 837.4099 | 837.4055 | 5.33 | 0 | 43 | 0.026 | 1Score > 40 indicates identity Score > 39 indicates homology |
U | K.NLADFMK.Q | |
| 1842 |
1 |
452.2373 | 1353.6901 | 1353.6816 | 6.23 | 1 | 64 | 1.8e-005 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | R.VQLDVKFDFDK.S + Deamidated (NQ) |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RP5M_PSEPK | 99 | Probable sigma(54) modulation protein OS=Pseudomonas putida (strain KT2440) GN=rpoX PE=3 SV=1 |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CAPA_PSEFR | 98 | Cold shock protein capA (Fragment) OS=Pseudomonas fragi GN=capA PE=2 SV=1 |
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