MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita3 sp |
| MS data file | : | PRT1270_T-BRSC_3_20250714120051.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:29 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 5,012 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPG | 110 | Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSE14 | 110 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL3_PSEE4 | 107 | 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1 |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LEU1_PSEP1 | 103 | 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CISY_PSEAE | 103 | Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_PSEE4 | 102 | Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 56.1 | IF2_PSEE4 |
102 | 91973 | 2 (2) | 2 (2) | 0.05 | |
| Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1 | |||||||
| 4 samesets of IF2_PSEE4 | |||||||
| IF2_PSEP1 |
102 | 91620 | 2 (2) | 2 (2) | 0.05 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=infB PE=3 SV=1 | |||||||
| IF2_PSEPG |
102 | 91294 | 2 (2) | 2 (2) | 0.05 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain GB-1) GN=infB PE=3 SV=1 | |||||||
| IF2_PSEPK |
102 | 91576 | 2 (2) | 2 (2) | 0.05 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1 | |||||||
| IF2_PSEPW |
102 | 91289 | 2 (2) | 2 (2) | 0.05 | ||
| Translation initiation factor IF-2 OS=Pseudomonas putida (strain W619) GN=infB PE=3 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2017 |
720.4193 | 1438.8240 | 1438.8144 | 6.71 | 0 | 67 | 4.4e-005 | 1Score > 36 indicates identity |
U | R.GPVATILVQDGTLR.Q | |
| 4314 |
973.8635 | 2918.5687 | 2918.5138 | 18.8 | 1 | 62 | 1.9e-006 | 1Score > 33 indicates identity Score > 18 indicates homology |
U | R.VIGGGVGGITESDANLALASNAVLFGFNVR.A + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| IF2_CAMHC |
67 | 100767 | 56.1 | ||
| Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1 | |||||
| 12 samesets of IF2_CAMHC | |||||
| IF2_CAMLR |
67 | 96725 | |||
| Translation initiation factor IF-2 OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=infB PE=3 SV=1 | |||||
| IF2_MICAN |
67 | 109614 | |||
| Translation initiation factor IF-2 OS=Microcystis aeruginosa (strain NIES-843) GN=infB PE=3 SV=1 | |||||
| IF2_CYAP4 |
67 | 108559 | |||
| Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=infB PE=3 SV=1 | |||||
| IF2_CYAP7 |
67 | 121749 | |||
| Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7424) GN=infB PE=3 SV=1 | |||||
| IF2_THEEB |
67 | 104469 | |||
| Translation initiation factor IF-2 OS=Thermosynechococcus elongatus (strain BP-1) GN=infB PE=3 SV=1 | |||||
| IF2_SYNE7 |
67 | 110858 | |||
| Translation initiation factor IF-2 OS=Synechococcus elongatus (strain PCC 7942) GN=infB PE=3 SV=1 | |||||
| IF2_SYNP6 |
67 | 110787 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=infB PE=3 SV=1 | |||||
| IF2_SYNP2 |
67 | 105884 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=infB PE=3 SV=1 | |||||
| IF2_SYNJA |
67 | 111852 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain JA-3-3Ab) GN=infB PE=3 SV=1 | |||||
| IF2_SYNJB |
67 | 113485 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. GN=infB PE=3 SV=1 | |||||
| IF2_SYNPW |
67 | 119022 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain WH7803) GN=infB PE=3 SV=1 | |||||
| IF2_SYNS3 |
67 | 118793 | |||
| Translation initiation factor IF-2 OS=Synechococcus sp. (strain CC9311) GN=infB PE=3 SV=1 | |||||
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SODF_PSEPK | 99 | Superoxide dismutase [Fe] OS=Pseudomonas putida (strain KT2440) GN=sodB PE=3 SV=3 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 100 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RP5M_PSEPK | 99 | Probable sigma(54) modulation protein OS=Pseudomonas putida (strain KT2440) GN=rpoX PE=3 SV=1 |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CAPA_PSEFR | 98 | Cold shock protein capA (Fragment) OS=Pseudomonas fragi GN=capA PE=2 SV=1 |
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